Sasa Kenig

Sasa Kenig
University of Primorska | UP · Faculty of Health Sciences (FVZ)

University of Ljubljana

About

38
Publications
6,253
Reads
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861
Citations
Citations since 2017
24 Research Items
645 Citations
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Introduction
Sasa Kenig currently works at the Faculty of Health Sciences (FVZ), University of Primorska. Sasa does research in metabolism and investigates responses of the human body to different diets or bioactive compounds on cellular/molecular level. Their current project is 'Ketogenic diet in obese'.
Additional affiliations
January 2012 - October 2016
Sincrotrone Trieste S.C.p.A.
Position
  • PostDoc Position
October 2010 - December 2011
ICGEB
Position
  • PostDoc Position

Publications

Publications (38)
Article
Full-text available
Topoisomerase I (TOP1) inhibitors are an important class of anticancer drugs. The cytotoxicity of TOP1 inhibitors can be modulated by replication fork reversal through a process that requires poly(ADP-ribose) polymerase (PARP) activity. Whether regressed forks can efficiently restart and what factors are required to restart fork progression after f...
Article
We report on an optimized protocol for the digestion of cellular RNA, which minimally affects the cell membrane integrity, maintaining substantially unaltered the vibrational contributions of the other cellular macromolecules. The design of this protocol allowed us to collect the first FTIR spectra of intact hydrated B16 mouse melanoma cells depriv...
Article
The ketogenic diet (KD) is being increasingly promoted as a strategy to fight obesity. Although the KD is effective for weight loss and weight control, comprehensive determination of its relationship with biochemical, physiological and psychological changes is still largely unexplored. We hypothesized that a 12-week KD (12KD) would significantly af...
Article
Objective A 12-week ketogenic diet was shown to have many beneficial effects in healthy obese adults, but it is not clear if the supply of micronutrients is adequate. Methods In 35 adult individuals with BMI above 30, the intakes of minerals and their serum levels were analyzed at baseline and at weeks 4 and 12 of the ketogenic diet intervention....
Article
Increased intestinal permeability has been shown to be involved in several diseases associated with low-grade chronic inflammation, including obesity and metabolic syndrome. In the last decade, growing evidence shows the beneficial effects of probiotic-containing food supplementation on these conditions. In this crossover intervention study on 28 a...
Conference Paper
Full-text available
Hericium erinaceus is an edible and medicinal mushroom with a vast spectrum of pharmacological activities. Traditionally, it has been used against diseases of the digestive tract and for the regeneration of nerves in neurodegenerative diseases. While the prevalence of neurodegenerative diseases and their associated cognitive deficits is highly asso...
Poster
Full-text available
In an 8-week double-blind study with two parallel groups in which eligible healthy participants were randomly assigned to H. erinaceus supplement group (N=18; 67% F, 33% M), and placebo group (N=14; 67% F, 33% M). A food supplement produced from H. erinaceus fruiting bodies by MycoMedica d.o.o., Slovenia was used in this trial with a 3,44 mg daily...
Article
Full-text available
Helichrysum italicum (Roth) G. Don (HI) and Helichrysum arenarium (L.) Moench (HA) are rich in polyphenols and their infusions have beneficial effects for patients with metabolic syndrome. To investigate whether these effects are mediated by the gut microbiota, we analysed the effects of daily consumption of HI or HA infusion on the composition of...
Preprint
Background X-ray Fluorescence (XRF) microscopy has become a widespread method to observe the location of elemental components within cell. Although it is becoming a widely used technique in many laboratories and synchrotron facilities, sample preparation for this microscopy remains one of the main concerns. Indeed, the information available to rese...
Article
Full-text available
Health impairments characteristic for metabolic syndrome such as increased body mass, a dysregulated lipid or glucose profile and elevated blood pressure can be reversed by appropriate lifestyle modifications. Supplementing the normal diet with herbal infusions is a promising strategy. We conducted a randomised double-blind comparative study in whi...
Article
Full-text available
Ethnopharmacological relevance Helichrysum italicum (HI) is a Mediterranean plant with well-reported use in traditional medicine for a wide range of applications, including digestive and liver disorders, intestinal parasitic infections, wound healing, stomach ache and asthma. However, little is known about the global mechanism behind its pleiotropi...
Article
Full-text available
The aim of this study was to assess changes in mental health, gut microbiota composition, and stress marker serum cortisol due to COVID-19 lockdown in asymptomatic individuals. Healthy adults participated in anthropometric measurements, blood and stool sample collection pre-lockdown and post-lockdown (n = 38, 63.2% females), lifestyle and psycholog...
Article
Full-text available
This study was focused on the creation of high-protein bars formulated using whey protein isolate (24%) and soy protein isolate (6%) as the sources of proteins; oat flakes and inulin, both abundant in dietary fibres, and creatine monohydrate and other minor ingredients (vitamin and mineral mixture, potassium sorbate) to achieve the requirements for...
Article
Full-text available
Helichrysum italicum is a Mediterranean plant with various pharmacological activities. Despite extensive reports on the bioactivity of the plant, its clinically studied applications have not yet been reviewed. The aim of our study was to gather information on the internal use of H. italicum and its bioactive constituents to determine its efficacy a...
Article
Full-text available
Helichrysum italicum is an aromatic plant with promising pharmacological activities. Bioactive compounds found in plants represent an important alternative treatment for weight loss and an infusion of H. italicum contains compounds which could have such effect. Our aim was, therefore, to investigate its acute effects on resting energy expenditure (...
Article
Full-text available
Due to limited data about the impact of lockdown on health status, the present study aimed to investigate the impact of COVID-19-related lockdown on changes in dietary habits, physical activity and serum markers in healthy adults. A total of 38 asymptomatic adults aged from 23 to 59 with a normal BMI (22.5 kg/m2) participated in baseline and post-l...
Article
Full-text available
Helichrysum arenarium (L.) Moench (abbrev. as HA) has a long tradition in European eth-nomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, Helichrysum italicum (Roth) G. Don (abbrev. as HI) is more common. Since infusions from both plants are traditionally used, we aimed to compare thei...
Technical Report
Full-text available
The new doctoral study Prevention for Health is a modern and innovative study programme in which we aim to develop holistic preventive approaches to the most important public health problems in today's society in Slovenia and around the world. The study combines the fields of kinesiology, nursing, dietetics and physiotherapy in accordance with scie...
Article
Full-text available
Objectives: Obesity and overweight are chronic disorders of multifactorial origin that are characterized by high oxidative status and by chronic activation of macrophages in peripheral tissues. Effective therapeutic approaches to lower inflammation and oxidative stress are currently of general interest. Royal jelly (RJ) is a functional food with a...
Article
Chili and its main active ingredient capsaicin have been shown to induce several favorable biological activities. In the present study we have tested the effect of three chili sauces mimicking popular commercially available chili sauces with an increasing amount of capsaicinoids on a broad range of serum biochemical markers in thirty healthy subjec...
Article
Full-text available
RECQ1 helicase has multiple roles in DNA replication, including restoration of the replication fork and DNA repair, and plays an important role in tumour progression. Its expression is highly elevated in glioblastoma as compared to healthy brain tissue. We studied the effects of small hairpin RNA (shRNA)-induced silencing of RECQ1 helicase on the i...
Article
Povzetek Olaparib, zaviralec poli (ADP-riboza) polimeraze (PARP) je prvo protitumorsko zdravilo, ki delujejo po principu sintezne smrtnosti, odobreno za uporabo v kliniki, in sicer za zdravljenje raka jajčnikov za bolnice z BRCA-mutacijo. Olaparib povzroči smrt celic z mutacijo genov BRCA1 ali BRCA2, ki sta pomembna pri popravljanju poškodb DNA s h...
Article
Full-text available
Abstract: Human genome is among individuals 99.9 % identical, there are, however, important differences in the remaining part. Several companies offer analysis of these small differences - genetic polymorphisms, for different purposes. For athletes, genetic testing is available to identify individuals with undiagnosed cardiac disorders or stronger...
Article
Full-text available
Background: Glioblastoma stem cells (GSC) have been extensively recognized as a plausible cause of glioblastoma resistance to therapy and recurrence resulting in high glioblastoma mortality. Abnormalities in the DNA repair pathways might be responsible for the inability of the currently used chemotherapeutics to eliminate the (GSC) subpopulation....
Article
Full-text available
Radiation damage of biological samples remains a limiting factor in high resolution X-ray microscopy (XRM). Several studies have attempted to evaluate the extent and the effects of radiation damage, proposing strategies to minimise or prevent it. The present work aims to assess the impact of soft X-rays on formalin fixed cells on a systematic manne...
Article
Maintaining the correct folding of cellular proteins is essential for preserving cellular homeostasis. Protein dishomeostasis, aberrant protein folding and protein aggregation are indeed involved in several diseases including cancer, aging-associated and neurodegenerative disorders. Accumulation of protein aggregates can also be induced from a vari...
Article
Cell growth and replication occurs in an orderly manner through a set of tightly coordinated physiological events, classified as G0, G1, S, G2 and M in conformity to their characteristics. In a previous work, by combining the results of flow cytometry (FC) using Propidium Iodide (PI) staining, PI-FC, and Fourier Transform Infrared Microspectroscopy...
Article
Full-text available
Here we present a new bonding protocol for SU-8 negative tone photoresist that exploits the chemical modifications induced in the resin by exposure to 254 nm (UVC) light. Fourier Transform Infrared microspectroscopy ([small mu ]-FTIR) was used to carry out a thorough study on the chemical processes and modifications occurring within the epoxy resin...
Article
The knowledge of cell cycle phase distribution is of paramount importance for understanding cellular behaviour under normal and stressed growth conditions. This task is usually assessed using Flow Cytometry (FC) or immunohistochemistry. Here we report on the use of FTIR microspectroscopy in Microfluidic Devices (MD-IRMS) as an alternative technique...
Article
Despite all the progress in cancer treatment, glioblastoma, the most malignant tumor of the central nervous system, remains a terminal disease and new therapeutic approaches are urgently needed. A combination of chemotherapy with modifications that lower the apoptotic threshold of cancer cells could be effective. Cathepsin L inhibition was suggeste...
Article
Transcripts of the lysosomal cysteine proteinase cathepsin L are spliced into five variants (L-A, L-A1, LA-2, LA-3 and L-B), which have similar stabilities but different translation efficiencies, thus potentially yielding diverse amounts of the protein. The aim of this study was to investigate whether the abnormally elevated expression of cathepsin...

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Projects (2)
Project
Our goal was to determine safety and health effects of 12-week ketogenic diet in obese. The aim of the study was a comprehensive determination of quality of body mass loss, metabolic states changes, physical and psychological performance changes, appetite, and inflammatory and hormones profile changes during and post a 12-week low energy ketogenic diet intervention.