Sarin Tadtong

Srinakharinwirot University · Faculty of Pharmacy

Clerodendrum chinense is a plant containing numerous secondary active metabolites. This study aimed to evaluate the antioxidant and anti-cancer activities of C. chinense flower ethanolic extract and identify the bioactive compounds. High-performance liquid chromatography was used to identify and quantify the bioactive compounds of the extract. The...

An actinomycete strain, AA8T, which produced a long straight chain of spores (verticillati type), was isolated from the rhizosphere soil of Mangifera indica in Bangkok, Thailand. A polyphasic taxonomic study was carried out to establish the taxonomic position of the strain. Strain AA8T formed a tight taxonomic position in the 16S rRNA gene tree wit...

Tirandamycin (TAM B) is a tetramic acid antibiotic discovered to be active on a screen designed to find compounds with neuroprotective activity. The producing strain, SBST2-5T, is an actinobacterium that was isolated from wastewater treatment bio–sludge compost collected from Suphanburi province, Thailand. Taxonomic characterization based on a poly...

Mulberry (Morus alba L.) fruits have been suggested to be a good nutritional source of alkaloids, phenolic compounds, flavonoids, and anthocyanins, which can provide health benefits. However, limited studies have addressed how changes in health promotion compounds and mulberry fruit quality are affected by harvest season. Therefore, phytochemical p...

The first line therapy of patients with Parkinson’s disease, a neurodegenerative disorder caused by the degeneration of dopaminergic neurons, is levodopa (L-dopa) given orally. Recently, the presence of natural L-dopa in the seed of Mucuna pruriens, a tropical legume in the Fabaceae family, was reported and it showed superior efficiency compared wi...

L-dopa, a dopaminergic agonist, is the gold standard for the treatment of Parkinson’s disease. However, due to the long-term toxicity and adverse effects of using L-dopa as the first-line therapy for Parkinson’s disease, a search for alternative medications is an important current challenge. Traditional Ayurvedic medicine has suggested the use of M...

An endophytic Streptomyces -like micro-organism, designated strain PRB2-1 T was isolated from root tissue of Epipremnum aureum (Linden and André) G.S. Bunting. The typical morphological and chemotaxonomic characteristics, i.e. the ability to produce straight spore chains directly on aerial mycelium and the presence of ll -diaminopimelic acid in cel...

Wound healing impairment due to a postponed, incomplete, or uncoordinated healing process has been a challenging clinical problem. Much research has focused on wound care, particularly on discovery of new therapeutic approaches for acute and chronic wounds. This study aims to evaluate the effect of the combination of quercetin and curcuminoids at t...

An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110T, was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110T was a member of the genus Streptomyces and showed the closest similarities to Streptomyces...

Ocimum gratissimum L. leaf oil exhibited many pharmacological properties. This study aimed to formulate and evaluate the physical and chemical stability of O. gratissimum leaf oil nanoemulsion. O. gratissimum leaf oil was extracted by hydrodistillation. The major component of the essential oil eugenol, was analyzed by UV-Vis spectrophotometry. Nano...

An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp. The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium. The typical chemotaxonomic properties of members of the genus S...

An endophytic actinobacterium, designated strain CA1R205 T , was isolated from the surface-sterilized root of Coffea arabica L. collected from Ratchaburi province, Thailand. The taxonomic position of this strain was evaluated using a polyphasic approach. The strain produced light yellowish brown to dark brownish black substrate mycelium and greyish...

An aerobic, non-motile, Gram-stain positive actinomycete, designated strain CA3R110 T , was isolated from the surface-sterilised root of Coffea arabica L. collected from Lampang Province, Thailand. 16S rRNA gene sequence analysis indicated that strain CA3R110 T is a member of the genus Streptomyces and showed the closest similarities to Streptomyce...

Alzheimer’s disease (AD) is a common neurodegenerative disorder. The number of patients with AD is projected to reach 152 million by 2050. Donepezil, rivastigmine, galantamine, and memantine are the only four drugs currently approved by the United States Food and Drug Administration for AD treatment. However, these drugs can only alleviate AD sympt...

A number of complementary medicines have been investigated for their neuroprotection and treatment of neurodegenerative diseases. This study aimed to analyze the chemical compounds and the neuroprotective activity Piper sarmentosum Roxb. fruit essential oil. The essential oil from the fruits of P. sarmentosum (Piperaceae) was extracted by hydrodist...

Moringa oleifera seed oil has been recognized for its benefits in relation to the skin. The objective of this study was to evaluate the chemical composition and antioxidant activity of moringa seed oil, to formulate a moringa seed oil cream, and to determine the efficacy of moringa seed oil cream in vivo. The chemical components of moringa seed oil...

Alzheimer’s disease (AD) is a common chronic neurodegenerative disorders. Melatonin (MLT) has been reported to be neuroprotective agent, and its modified structures exhibit potent antioxidant and anti-inflammation activities. Therefore, the activity of MLT and its derivatives against AD was investigated. Herein, the targeted enzymes, such as β-secr...

Citrus hystrix DC (kaffir lime) leaf oil exhibited antifungal activities against Aspergillus niger and Candida albicans. This study aimed to evaluate the antifungal activity of kaffir lime leaf oil and microemulsions containing kaffir lime oil against Trichophyton mentagrophytes var. interdigitale. The chemical components of kaffir lime leaf oil we...

The aim of this study was to determine the prevalence of quality herbal products sold in Thailand. The quality of herbal products focuses on three specific issues: i) the legality of the labelling on herbal products, ii) microbial contamination and iii) corticosteroid adulteration in herbal products. This cross-sectional study randomly selected 431...

Mucuna pruriens is a tropical leguminous plant containing L-dopa which is the main active ingredient for Parkinson’s disease therapy. The objective of this study was to investigate the physical, chemical, and microbiological stability of M. pruriens seed extract effervescent powders and suspension. The effervescent powders and suspension were prepa...

An actinomycete strain CSR-4 was isolated from the rhizosphere soil of Zingiber montanum. Taxonomic characterization revealed strain CSR-4 was a member of the genus Microbispora. Whole-genome sequence analysis exhibited the highest average nucleotide identity (ANI) value (95.34%) and digital DNA-DNA hybridization (DDH) value (74.7%) between strain...

Free radicals are one cause of wrinkles. Adding antioxidants to the skin is one option to decrease wrinkles or slow the aging process. Up to now, research in aromatherapy product development for antioxidants has rarely been published. Therefore, the objectives of this study were: (i) to analyze the chemical composition of blended essential oil form...

A novel actinomycete, strain RTBAU4-9 T , which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such as meso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amo...

Eight previously undescribed naturally-occurring compounds, including abyssomycins Y - Z, methyl aeruginoate, desferri-ferrithocin-4-hydroxyphenethylester, streptomethiocins A - B, furaquinocin I, and streptolactone, along with eleven known compounds were isolated from the endophytic Streptomyces sp. TBRC7642. The chemical structures were determine...

Nine new chromone analogs (1–9) were isolated from the soil actinomycete Microbispora sp. TBRC6027. The chemical structures were determined based upon NMR spectroscopic methods. These compounds were evaluated in vitro by using P19-derived neurons for neuroprotective activity against oxidative stress induced by serum deprivation and showed % viabili...

Nine new chromone analogs (1-9) were isolated from the soil actinomycete Microbispora sp. TBRC6027. The chemical structures were determined based upon NMR spectroscopic methods. These compounds were evaluated in vitro by using P19-derived neurons for neuroprotective activity against oxidative stress induced by serum deprivation and showed % viabili...

The essential oil from rhizomes of Curcuma alismatifolia Gagnep. was extracted by hydrodistillation. Chemical analysis by gas chromatography-mass spectrometry identified 12 compounds, of which the most prominent were xanthorrhizol (82.2%), ar-curcumene (6.5%), α-cedrene (1.8%), and β-bisabolol (1.1%). The essential oil at 1 ng/mL showed neuroprotec...

An endophytic actinobacterium, designated strain KE2-3T, was isolated from surface-sterilised rhizome of Kaempferia elegans. The polyphasic approach was used for evaluating the taxonomic position of this strain. The taxonomic affiliation of this strain at genus level could be confirmed by its chemotaxonomic characteristic, i.e. the presence of ll-d...

A novel actinomycete, strain RTBAU4-9T, which produced longitudinally paired spores on aerial mycelia, was isolated from a soil sample of a hot spring area. This strain exhibited chemotaxonomic and genotypic properties typical of the genus Microbispora, such asmeso-diaminopimelic acid in the cell-wall peptidoglycan, glucose, ribose and trace amount...

A novel actinomycete, strain PLAI 1-1T, which formed spiny single spore directly on substrate mycelium was isolated from root tissue of Zingiber montanum. The isolate contained meso-diaminopimelic acid and 3-hydroxydiaminopimelic acid in the cell-wall peptidoglycan. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell sugars of...

Upland rice bran is an abundant and valuable antioxidant by-product of rice milling. This study compares the content of γ-oryzanol, γ-tocopherol, and phytochemicals in rice bran oil from 4 varieties of upland rice; Dok-kham (DK), Dok-kha (D), Khem-ngen (KN), and Nang-dam (ND); with that of a conventional variety, Khao Dowk Mali 105 (MA). The extrac...

Blended essential oil comprising rose, bergamot and patchouli (RBP) oils with the volume ratio of 6:3:1, respectively have been tested for various biological activities. The blended oil showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis with minimum inhibitory concentration (MIC) of 0.003125 %v/v and 0.125...

Context: Pluchea indica (L.) Less (Asteraceae) is an herb used as a traditional medicine for wound healing. The chemical compounds found in Pluchea indica leaves are phenolic acids, flavonoids, anthocyanins and carotenoids. Objective: This study investigates the effect of Pluchea indica leaf ethanol extract and its nanoparticles (NPs) on cytotoxici...

A series of prenylated resveratrol derivatives were designed, semisynthesized and biologically evaluated for inhibition of β-secretase (BACE1) and amyloid-β (Aβ) aggregation as well as free radical scavenging and neuroprotective and neuritogenic activities, as potential novel multifunctional agents against Alzheimer’s disease (AD). The results show...

The leaves of Aquilaria crassna (Thymelaeaceae) are recently become an attractive research due to their potential in antioxidative, antibacterial, antipyretic, analgesic, and laxative activities. However, the neuritogenic and neuroprotective effects of A. crassna leaves have never been reported. In this study, three phenolic glycosides were isolate...

Objective: The aim of this study was to develop a stability indicating method to determine bioactive nucleosides including uridine, guanosine, adenosine, and cordycepin in crude drugs, extracts, and products from Cordyceps sinensis and Cordyceps militaris by reverse phase high performance liquid chromatography. Methods: The C8 column (250 mm × 4.6...

Alzheimer’s disease (AD) is a common neurodegenerative disorder, one of the hallmarks of which is the deposition of aggregated β-amyloid peptides (Aβ40,42) as plaques in the brain. Oligomers of these peptides have been reported to be toxic and to inhibit neurite outgrowth, as evidenced by neurite dystrophy and significant loss of synaptic connectiv...

Purpose: To modify the structure of oxyresveratrol and evaluate the obtained derivatives for effects on neuronal cells. Methods: Electron-withdrawing groups were selectively introduced to the aromatic ring of the core stilbene structure. Oxyresveratrol and derivatives were then evaluated for their ability to enhance the survival of P19 derived neur...

The induction of lymphangiogenesis is an important process to promote cancer growth and cancer metastasis via the lymphatic system. Identifying the compounds that can prevent lymphangiogenesis for cancer therapy is urgently required. Chrysin, 5,7-dihydroxyflavone, a natural flavone extracted from Thai propolis, was used to investigate the effect on...

The leaves of Garcinia gracilis Pierre, Clusiaceae, have been used as flavouring materials in food, with no previous reports of their biological activities and chemical constituents. In this study, the methanolic extract of G. gracilis afforded three compounds namely apigenin-8-C-α-l-rhamnopyranosyl-(1→2)-β-d-glucopyranoside (1), 5-hydroxymethyl-2-...

The synergistic antimicrobial activities of blended essential oil preparations composed of eucalyptus, rosemary, patchouli, pine and cajuput oils were evaluated against various pathogenic microorganisms. They exhibited antimicrobial activity in the agar disc diffusion assay against the Gram-positive bacteria, Staphylococcus aureus and S. epidenidis...

Historically, rice has been served as a food and as an herbal remedy. The claimed therapeutic properties of rice-derived products have attracted a considerable amount of attention, but these therapeutic properties are currently underestimated. Rice panicles of Phitsanulok 2 (PL), Suphan Buri 1 (SP) and San Pa Tong 1 (ST) varieties were compared bas...

The essential oil of C. oblongifolius Roxb. stem bark was obtained by hydrodistillation. Chemical analysis by GC-MS identified 29 compounds. Terpinen-4-ol (17.8%) was a major component, together with α-guaiene (7.9%), E-caryophyllene (7.0%), myrcene (6.7%), (+)-cyclosativene (5.1%), sabinene (4.8%), aciphyllene (4.7%), pogostol (4.6%), -terpinene...

This study focused on characterization of the chemical components of an aromatherapy recipe. The formulation consisted of four blended essential oils; rosemary oil, eucalyptus oil, pine oil and lime oil (volume ratio 6 : 2 : 1 : 1). The single and combination essential oils were identified by gas chromatography-mass spectrometry (GC-MS). The analys...

Essential oils have the strong potency to inhibit acetylcholinesterase (AChE) in their lipophilicity and small molecular size constituents. Cholinesterase inhibitory activity was determined against AChE using Ellman's colorimetric method of 29 essential oils from aromatic plants. Results revealed 5 of essential oils from Eucalyptus, Cajuput, Sweet...

From the fresh leaf sheathes of lemongrass (Cymbopogon citratus) and rhizomes of galanga (Alpinia galanga) light yellow and colorless oils, respectively, were obtained by hydrodistillation and microwave assisted extraction (MAE) in yields of 0.24% and 0.03%, and 0.11% and trace (w/w), respectively. By GC/MS analysis, five major constituents were id...

Background and Objectives: Due to the volatility of alcohol, the concentration remaining of 70% alcohol after opened is suspected. To ensure that the solution is appropriate for use, it is necessary to test the stability of the prepared products after opened and an expiry date can be judged. Materials and Methods: This chemical stability study was...

Blended essential oil formula for anti-oxidation and anti-wrinkle contains lavender (Lavandula angustifolia Mill.), clary sage (Salvia sclarea L.), and ylang-ylang (Cananga odorata (Lam.) Hook. F. & Th.) oils. The blended essential oil formula is prepared in a volume ratio. The formula is composed of lavender:clary sage oils:ylang ylang (2:4:4). Th...

Neuritogenic and neuroprotective activities of litchi (Litchi chinensis Sonn., Sapindaceae) and salacca (Salacca edulis Reinw., Arecaceae) pericarp, and sapodilla (Achras sapota L., Sapotaceae) and tamarind Srichompu cultivar (Tamarindus indica L., Caesalpiniaceae) seed coat extracts were evaluated on cultured cholinergic P19-derived neurons. All t...

Coumarins have been identified from natural sources, especially green plants such as Clausena harmandiana. Antioxidant activity of the plant was assayed using 1,1-diphenyl-2-pricrylhydrazyl (DPPH) and Thiobarbituric Acid Reactive Substances (TBARS) methods while its neuritogenic effects was assessed on cultured P19 neurons. Nordentatin, isolated fr...

Quercetin has been isolated for the first time from ethyl acetate extract of Caesalpinia mimosoides Lamk. C. mimosoides Lamk. (Fabaceae) or Cha rueat (Thai name) is an indigenous plant found in mixed deciduous forest in northern and north-eastern parts of Thailand. Thai rural people consume its young shoots and leaves as a fresh vegetable, as well...

Antimicrobial activities of two blended essential oil preparations comprising lavender oil, petigrain oil, clary sage oil, ylang ylang oil and jasmine oil were evaluated against various pathogenic microorganisms. Both preparations showed antimicrobial activity in the agar disc diffusion assay against the Gram-positive bacteria, Staphylococcus aureu...

The purpose of this study is to determine the chemical compositions and the antimicrobial activities of the essential oil from rhizome of Etlingera punicea (Roxb.) R.M. Smith. The rhizome of plant is used as spice and ingredient in noodle and curry in Thailand. Essential oil from rhizome of the plant was extracted by hydrodistillation and analyzed...

The objective of this study is to determine the tyrosinase inhibition and antibacterial activities against the pathogenic bacteria in the oral cavity of the mangosteen pericarp extract. The results showed that the mangosteen pericarp extract inhibited the tyrosinase enzyme at IC50 = 67 ng/ml. Furthermore, the mangosteen pericarp extract also exhibi...

Geldanamycin (1), an antifungal and anticancer ansamycin, was reported as a neurotrophic and neuroprotective substance against antineoplastic drugs, paclitaxel, vincristine, and cisplatin, on cultured dorsal root ganglion neurons from chick embryos. In this study, 1 in a large quantity, together with a known 17-O-demethylgeldanamycin (2), and a new...