Sardul Singh Sandhu

Rani Durgavati Vishwavidyalaya · Design Innovation Center, Department of Biological Sciences

Nanobiotechnology is the innovative field of science which involves biological synthesis, characterization and application of numerous nanoparticles. The present study was planned to explore potential of endophytic fungi for eco-friendly synthesis of silver nanoparticles with antibacterial activity. In this study 8 endophytic fungal isolates were o...

In present study Entomopathogenic fungi Beauveria bassiana was used as biological agent of synthesizing silver nanoparticles that have tested for its antibacterial activity against different pathogenic bacterial strains .It showed maximum activity against Escherichia coli (6mm) followed by Bacillus subtillis (TCBS) (5mm),Salmonella typhimurium (XLD...

The globe is currently confronting a global fight against the deadliest cancer sickness. Chemotherapy, hormonal therapy, surgery, and radiation therapy are among cancer treatment options. Still, these treatments can induce patient side effects, including recurrence, multidrug resistance, fever, and weakness. As a result, the scientific community is...

The globe is currently confronting a global fight against the deadliest cancer sickness. Chemotherapy, hormonal therapy, surgery, and radiation therapy are among cancer treatment options. Still, these treatments can induce patient side effects, including recurrence, multidrug resistance, fever, and weakness. As a result, the scientific community is...

Cancer represents the second most deadly disease and one of the most important public health concerns worldwide. Surgery, chemotherapy, radiation therapy, and immune therapy are the major types of treatment strategies that have been implemented in cancer treatment. Unfortunately, these treatment options suffer from major limitations, such as drug-r...

The world is currently facing a global challenge against neoplastic diseases. Chemotherapy, hormonal therapy, surgery, and radiation therapy are some approaches used to treat cancer. However, these treatments are frequently causing side effects in patients, such as multidrug resistance, fever, weakness, and allergy, among others side effects. As a...

Nowadays, microorganisms are capable of resolving many crises. Microbial consortia are the mixture of specific microorganisms that help us solve any particular problem. It includes bioremediation and degradation of any toxic compounds like polyethylene, hydrocarbon, chemical dyes, as well as crafting a microenvironment and boosting plant growth, se...

For thousands of year dairy products have been the major parts of human diet because fermented milk products like curd, cheese, and butter could be better preserved than the fresh raw milk from which they were made (Kok & Hutkins, 2018). Most fermented dairy products naturally contain organic acids, ethanol, or other microbial compounds that inhibi...

The investigation was carried out to study the response of wheat and associated weed species against Elevated carbon dioxide. The weed species selected for the study were Phalaris minor and wild oat. The experiment was conducted under Ambient and Elevated CO2 conditions. The physiological parameters including Relative stress injury, Chlorophyll ‘a’...

Purpose of Review Nowadays, the need of novel biological active compounds is growing as there is remarkable increase in antibiotic resistance developed in microbes. In the present time, the need of new active novel biological molecules is growing as there is remarkable increase in antibiotic resistance. Therefore, the main purpose of this review ar...

Scavenging free radical potency of cordycepin is the major bioactive segment extricated from Cordyceps species. In some new years, Cordyceps has gotten growing thought inferable from its distinctive restorative/pharmacological tests. This assessment reviews continuous explores on the counter oxidant impacts and the associated analyses of Cordyceps...

Scavenging free radical potency of cordycepin is the major bioactive segment extricated from Cordyceps species. In some new years, Cordyceps has gotten growing thought inferable from its distinctive restorative/pharmacological tests. This assessment reviews continuous explores on the counter oxidant impacts and the associated analyses of Cordyceps...

Purpose of Review Endophytes such as bacteria, fungi and actinomycetes are play significant role in the production of bioactive metabolites and plant defence mechanisms. These endophytes develop asymptomatically in the inner tissues and cells of the host plant without causing any symptoms. But studies are ongoing on endophytic fungi, since many of...

Increasing industrialisation, continuous population growth and high demand of petroleum products have led to effects economic growth and development of a country. The dependency on fossil fuels has resulted in serious ecological issues and causes adverse impact on the human health have been seen over recent decades. Therefore, a number of different...

In our daily life, the demand for liquid petroleum products is increasing day by day. Crude oil-derived hydrocarbons, the largest group of environmental pollutants found worldwide, pollute our environments severely. Oil or hydrocarbons cause drastic impacts on living organisms. The many reports about their toxicity emphasize the ultimate need to re...

Soil remediation is a term that involves a numerous processes designed to get rid of contaminants like hydrocarbons (petroleum and fuel residues), heavy metals, pesticides, cyanides, volatiles, or semi-volatiles from soil. Remediation is required to control the pollution in soil, water, and air that can consequently benefit commercial cultivation o...

Globalization is increasingly changing the lifestyle of the people and their aspirations. Colors are desirable and play a large part in human life. The synthetic colors are commonly used in the various food processing, painting, plastics, pharmaceutical, textile, and cosmetics industries, and are more attractive, reliable, and cost-effective compar...

Purpose of Review In the present review, two strain improvement strategies viz. transformation system development and inter-specific protoplast fusion for this mushroom are hypothesized. Recent Findings The entomopathogenic medicinal mushroom Cordyceps species has been bestowing health biologically and pharmacologically for years. It harbors a var...

The miraculous medicinal effect of secondary metabolite produced from Cordyceps Sp., known for traditional use to cure deadly diseases. Ophiocordyceps sinensis and their best alternate Cordyceps militaris is widely using for the medicinal purposes in pharmaceutical industries. In the present research both the entomopathogenic fungal metabolites wit...

Despite advancements in healthcare facilities for diagnosis and treatment, cancer remains the leading cause of death worldwide. As prevention is always better than cure, efficient strategies are needed in order to deal with the menace of cancer. The use of phytochemicals as adjuvant chemotherapeutic agents in heterogeneous human carcinomas like bre...

Natural drugs play extensive role and are the basis of traditional systems for cure and treatment of diseases. Entomopathogenic fungi Cordyceps Spp. are one of the unique and valuable sources of bioactive compounds which help in treatment of various diseases like nervous disorders, cardiovascular diseases, tumors, ageing, hypo-sexuality, etc. A sig...

Objective: The main purpose of the present study was to isolate the endophytic fungi from Tridax procumbens L. and evaluate their antibacterial activity at different concentration of compatible solvents extracted metabolites against the test pathogenic bacterial strain, as well as the molecular characterization of potent endophytic fungal isolate t...

The growing commercialization all over the world has led to a boost in the widespread use of chemical pesticides for crop protection in agricultural fields. It has not only contributed to an increase in food production, but its toxic and non-biodegradable character has also resulted in adverse effects on environment and nontarget organisms. Moreove...

The current research focuses on the isolation of bioactive compounds from the natural sources which have immense potential for pharmaceutical value. Pharmaceutical biology perceives plants as a unique resource of potentially precious remedial bioactive metabolites. But due to slow growth and harvest of endangerd plants species pose a threat and inb...

Biodegradation is a cost effective and eco-friendly treatment for oil-contaminated materials by the use of microorganisms. The principal hydrocarbon degrading microorganisms are bacteria and fungi. Microorganisms can degrade oil and petroleum as an energy source and this may provide an enormous scope to remediate soils contaminated with petroleum a...

Adequate expression of Bt (Bacillus thuringiensis) toxins and purity of seeds of Bt-transgenic cottons are important for controlling bollworms, and thereby increasing the cotton productivity. Therefore, we examined the variability in expression of Bt toxin proteins in the seeds and in leaves of different cotton (Gossypium hirsutum (L.) hybrids (JKC...

Chitinases occur in a wide range of organisms including bacteria, fungi, plants, insects, and animals. Chitinase has been widely used as one of the mycobiocontrol agent as it degrades chitin which is a chain homopolymer of N- acetylglucosamine (GlcNAc) connected by β-1,4 glucosidic linkages. Beauveria bassiana is an insect pathogenic fungus success...

Metal oxide nanoparticles (MO-NPs) are the multidisciplinary nano-scaled molecules which are being used in the diagnosis and treatment of the challenging diseases including cancer. Evidences suggest that antimicrobial formulations in the form of MO-NPs can be possibly used as effective antimicrobial agents. In addition, MO-NPs are known to target v...

Hormones are a class of molecules, which mediate their effects by regulating a variety of signalling pathways. Melatonin (N-acetyl-5-methoxytryptamine), a pineal gland hormone, is one among the categories of compounds having various therapeutic and pharmacological effects. Melatonin has many intracellular as well as extracellular targets including...

In recent years, growing interest has been focused on the field of chemoprevention using natural therapies. The reason to turn toward "natural" remedies is associated with diverse beneficial pharmacological properties of natural compounds. Isothiocyanates (ITCs), the major pharmacological active constituents of cruciferous vegetables, are derived f...

Most of the pathogenic bacterial strains have developed resistance against the commonly used antibacterial drugs. This problem is increasing at an alarming rate and has urged upon the need to search for novel antibacterial compounds from natural sources. Entomopathogenic fungi have proved to be very promising candidates as they are known to produce...

Ailanthus excelsa Roxb. is one of the most important plant which has a specific place in Ayurveda and phytotherapy. The tribal people generally used this plant for anthelminthic, anti-fertility, rejuvenation and anti-malarial against Plasmodium falciparum. In the present study total four endophytic fungi were isolated from the leaves of medicinal p...

Despite numerous efforts by researchers the increasing fungal infections are still a major threat to the society. To combat this dilemma several antifungal drugs have come up through extensive research. Azoles are one of the most promising antifungal agents of them. They are heterocyclic five membered organic compounds which show activity against h...

DIAGNOSIS OF CANCER USING ARTIFICIAL NEURAL NETWORK AND CLOUD COMPUTING APPROACH Ishwinder Kaur Sandhu, Meera Nair, Ravindra Prasad Aharwal and Sardul Singh Sandhu 1 Department of Computer Science and Engineering, Oriental College of Technology, Patel Nagar, Raisen Raod, Bhopal, M.P., India. 2Centre for Scientific Research & Development, People’s U...

With the recent emergence of novel technologies, the field of biomarker discovery has been the subject of intense research and activity. Major advances in cancer control will be greatly aided by early detection for diagnosing and treating cancer in its pre-invasive state. Cancer being a diverse disease involves alterations in three classes of genes...

Endophytes are those organisms that colonize the living internal tissues of their hosts without causing any visible symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved as a rich source of bioactive compounds. Calotropis procera (Linn.) R.Br. a widely used medicinal plant in India, were explo...

Endophytic fungi reside inter or intracellularly in plant parts for at least a portion of their lives without causing apparent symptoms of infection. Endophytic fungi are ubiquitous fungi. All plants in the natural environment can shelter endophytic fungi, including algae, mosses, ferns, conifers and angiosperms. Endophytic fungi are an important s...

Cordycepin, a nucleoside derivative, is an active component of parasitic fungus, Cordyceps militaris which has been known for many pharmacological activities. The present study was carried out to develop an extraction strategy for cordycepin and to evaluate its antimicrobial potential. Solvent- solvent extraction method was used to extract cordycep...

Objective: Cordyceps militaris is an entomopathogen and known to exhibit significant therapeutic potential. In the present study, we aimed to extract various fractions (aqueous; hexane; chloroform & butanol) including active ingredient cordycepin from fermented broth of Cordyceps militaris followed by their evaluation as anti-angiogenic agents. Met...

Cordycepin, an active ingredient in the insect fungus Cordyceps militaris, is in a category of compounds that exhibit significant therapeutic activity. The aim of the present study was to investigate the effect of cordycepin on cell proliferation, apoptosis, and cell cycle in A549 human lung cancer cells. MTT assay was used to evaluate the cytotoxi...

Cordycepin, an active ingredient of the insect fungus Cordyceps militaris, is a category of compounds that exhibit significant therapeutic potential. The aim of this work was to optimize maximum cordycepin production conditions in fermented broth of C. militaris 3936 The suitable physical and nutritional conditions for cordycepin production were in...

Endophytes are those organisms that colonize the living internal tissues of their hosts without causing any visible symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved as a rich source of bioactive compounds. Calotropis procera (Linn.) R.Br. a widely used medicinal plant in India, were exploited...

Objective: The aim of the present investigation is to examine the collected wild mushroom for their in-vitro antibacterial activity and cultivation of potential mushroom strains.

Flavonoids are substances which are widely distributed in the plant kingdom. They occur in considerable quantities in common food products, spices and beverages. Interest of researchers has been greatly enhanced towards therapeutic potential of flavonoids in last some years. This review summarizes important patents pertaining to recent biotechnolog...

All organisms are protected by their immune system but occasionally this system overreacts leading to allergic and inflammatory reactions in the body. These reactions are the starting points of various diseases like asthma, eczema and urticaria etc. They involve activation of T cells and release of histamines and toxic chemicals from mast cells and...

An entomopathogenic fungus, Cordyceps sp. has been known to have numerous pharmacological and therapeutic implications, especially, in terms of human health making it a suitable candidate for ethno-pharmacological use. Main constituent of the extract derived from this fungus comprises a novel bio-metabolite called as Cordycepin (3′deoxyadenosine) w...

Stem cells are at the forefront of biomedical innovation, promising new ways to repair and replace damaged cells and tissues. Human stem cell research has contributed to the understanding of human biology, but their exist evidence from animal studies that stem cells can be made to differentiate into cells of choice in their transplanted environment...

Trypsin inhibitors (TI) have crucial functions in plant defense against pests, and recent studies reveal their diversity in stress response. Disease and herbivore infestations are harmful to plant growth, but the response of TI in Cicer arietinum (L.) in Central India has been less studied. In the present study, we partially characterized the TI fr...

The growing demand for reducing chemical inputs in agriculture and increased resistance to insecticides have provided great impetus to the development of alternative forms of insect-pest control. Myco-biocontrol offers an attractive alternative to the use of chemical pesticides. Myco-biocontrol agents are naturally occurring organisms which are per...

A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen for the transformation experiments....

Wounds are the result of injuries to the skin that disrupt the other soft tissue. Healing of a wound is a complex and protracted process of tissue repair and remodeling in response to injury. Various plant products have been used in treatment of wounds over the years. Wound healing herbal extracts promote blood clotting, fight infection, and accele...

Tuberculosis is a major global health challenge and is far from being controlled. Development of resistance to currently available drugs due to the successful adaptation of the pathogen has been a major contributing factor for its control failure. Presently, there is an immense interest in identification of pathways, unique to the intracellular env...

The present investigation deals with designing of savlon loaded blend hydrogels (termed cryogels) of polyvinyl alcohol (PVA) and alginate by repeated freeze–thaw method and their characterization by SEM and FTIR techniques. The FTIR spectra clearly reveal that savlon loaded alginate and PVA blends are bonded together through hydrogen bonding. The S...

A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genetic relatedness among isolates of the genus Phoma. Randomly Amplified Polymorphic DNA (RAPD) revealed the presence of interspecific genetic variation among the pigment producing isolates of Phoma and has shown distinct phylogenetic cluste...

Traditional taxonomy of hyphomycetes has been based on conidial morphology and development. In order to confirm species level for the detection and identification of the entomopathogenic fungus, we analysed the species-specific fingerprints to investigate molecular characteristics within isolates of six species and to resolve morphologically atypic...

Consumption of liquid oral medicine by frequently ill children, significantly increases the risk of tooth demineralization. In this study a survey was conducted wherein the pediatricians, pharmacist and medical representatives of Bhopal city were enquired about the most commonly prescribed liquid oral medicines for children. The four categories of...

We report here that, during periodical surveys of insects inhabiting diverse habitats for the collection of entomopathogenic fungi; a large number of isolates were recovered belonging to seven species, from various regions of Madhya Pradesh and Chhattisgarh forest areas and agricultural fields. The most common entomopathogenic fungi such as Beauver...

Bioproducts of Mushroom have multi beneficial effects for human welfare. Medicinal mushrooms are widely used as traditional medicinal ingredients for the treatment of various diseases and related health problems. Most of the medicinal extracts from mushroom are different forms of polysaccharides which strengthens the immune system with little or no...

With 1 figure and 1 table Curcuma longa L. is a sterile, triploid, vegetatively-propagated crop cultivated mainly in Southeast Asia. When dried rhizomes are ground, the resulting yellow powder is used by the food industry as a natural food dye. Moreover, many pharmacological compounds have broadened the commercial application of the crop. However,...

Stem Cell has become a booming field for research and therapeutic applications with vast areas yet to be discovered. The pluripotent nature of the stem cells has been studied and used in various scientific fields for curing the disease and regeneration of a body part. Periodontitis, dental caries, craniofacial bone and teeth regeneration are the di...

The present study focuses on the assessment of the antibacterial potential of two species of genus Agaricus from India. Three strains of A. bisporus and two strains of A. bitorquis were tested against six species of pathogenic and nonpathogenic bacteria. Antibacterial activity was detected in the culture filtrate of these two mushrooms but not in a...

Interspecies fusants are formed between Agaricus bisporus and Agaricus bitorquis by protoplast fusion technique. Protoplasts were isolated and regenerated by using Novozyme 234 lytic enzyme. Twenty slow growing isolates were separated from the protoplast regenerated colonies, which were assumed as homokaryons (putative homokaryons). These twenty is...