Sarath Josh Moolakkariyil

• M.Sc., Ph.D.
• Research Associate at Mahatma Gandhi University

Towards a process development for the bioremediation of phthalic acid esters (PAE) blended in various plastics, we explored the type of PAE in commercially available PVC blood storage bags (BB), its solvent- and temperature-dependent migrations and coefficient of diffusion (D) behaviors at static condition by solvent extraction method. The PAE [abo...

This exhaustive in silico study looks into the molecular interactions of phthalates and their metabolites with human peroxisome proliferator-activated receptor (hPPAR) and retinoid X receptor (hRXR) α, β and γ subtypes - the nuclear receptor proteins function as transcription factors by regulating the expression of downstream genes. Apart from the...

Being lipophilic xenobiotic chemicals, phthalates from the surrounding environments can easily be absorbed into the biological system, thereby causing various health problems including cancer and endocrine disruption in test animals and also in humans. In the present in silico study employing Glide, Schro¨dinger Suite 2012, we analysed in detail th...

Phthalic acid esters or phthalates are ubiquitous environmental pollutants known for their adverse health effects in test animals and, of late, in humans. Thus, in this molecular docking study - using Glide (Schrödinger) - the molecular interactions of 31 ligands, including 12 diphthalates, their monophthalates and phthalic acid with selected human...

The leachability of di(2-ethylhexyl)phthalate (DEHP), a phthalate plasticizer known for their adverse health effects in test animals and in humans, from commercial polyvinyl chloride (PVC) blood bag into aqueous organic solvents such as acetonitrile:water and methanol:water were studied. The effect of temperature and solvent composition were also s...

his study describes a novel dark-green spore producing strain of Trichoderma harzianum exhibiting higher activities of cellulase, hemicellulase and ligninase on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was supplemented with synthetic [carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, lact...

Phthalates are known to cause endocrine disruption in humans and animals. Being lipophilic xenobiotic chemicals, phthalates from the surrounding environments can easily be absorbed into the biological system, thereby causing various health dysfunctions. This molecular docking study evaluates a variety of molecular interactions of 12 commonly used d...

The present study describes the culture characteristics, isolation and partial purification of a yellow pigment produced by a novel bacterium, Micrococcus luteus strain BAA2. The growth conditions of M. luteus strain BAA2 were optimized. It showed the optimum growth (OD: 0.55 at λ660) at 28ºC (pH 7, 24 h), while the maximum pigment (OD: 0.50 at...

Abstract Production of an esterase by Achromobacter denitrificans strain SP1 – a di(2-ethylhexyl)phthalate (DEHP) degrading novel bacterium – in a modified basal salt medium supplemented with DEHP as an inducer-cum-additional carbon source was studied. The Plackett–Burman and Box–Behnken designs were applied to statistically optimize the production...

The endocrine disrupting phthalates known for their adverse human health effects are illustrious environmental pollutants. The present in silico molecular docking studies explored the molecular interactions of 31 ligands including 12 diphthalates, 12 monophthalates, phthalic acid and bisphenol A (BPA) with human steroid receptors, and compared thei...

Phthalates or phthalic acid esters are a group of xenobiotic and hazardous compounds blended in plastics to enhance their plasticity and versatility. Enormous quantities of phthalates are produced globally for the production of plastic goods, whose disposal and leaching out into the surroundings cause serious concerns to the environment, biota and...

Solid-state fermentation (SSF) holds tremendous potentials for the production of industrially significant enzymes. The present study describes the production of lipase by a novel rumen bacterium, Pseudomonas sp. strain BUP6 on agro-industrial residues. Pseudomonas sp. strain BUP6 showed higher lipase production when grown in Basal salt medium (BSM)...

This study describes how Achromobacter denitrificans strain SP1, a novel isolate from heavily plastics-contaminated sewage sludge efficiently consumed the hazardous plasticizer, di(2-ethylhexyl)phthalate (DEHP) as carbon source supplemented in a simple basal salt medium (BSM). Response surface methodology was employed for the statistical optimizati...

This first report describes the purification and identification of an orange-red pigment produced by Achromobacter denitrificans strain SP1 (isolated from sewage sludge heavily contaminated with plastics) during its growth in a simple basal salt medium supplemented with the hazardous di(2-ethylhexyl)phthalate (DEHP) blended in PVC blood bag (in sit...

This study describes how the microbes (bacteria and fungi) efficiently bioremediate di(2-ethylhexyl)phthalate (DEHP), the harmful plasticizer blended in polyvinylchloride (PVC) plastics. DEHP is only physically bound to the polymer network of PVC, so it can easily migrate into the surrounding environments, which in humans shown to cause many health...

Abstract In the modern world, variety of pollutants especially plastic wastes requires specific processing techniques for its remediation and recycling. Among these plastic wastes polyvinyl chloride wastes needs special attention since the incineration cause dioxin production and land filling results in hazardous phthalates. Phthalates (phthalic a...

After giving a glimpse into the global market of bioplastics and briefly describing the various forms of bioplastics, this chapter presents a detailed discussion on polyhydroxybutyrate (PHB), the predominant polyhydroxyalkanoate produced by bacteria as stored food. This review encompasses interdependent sections on the organization of the PHB granu...

In this study, we explored the efficacy of raw potato flour (PF) as supplement to the conventional LB medium (LB control, designated as M1) for enhancing the concomitant production of endospores and δ-endotoxin from Bacillus thuringiensis subsp. kurstaki by solid-state fermentation (SSF). Of different concentrations and combinations of media tested...

This study describes a novel dark-green spore pro-ducing strain of Trichoderma harzianum exhibiting higher activities of cellulase, hemicellulase and ligni-nase on specific plate assays. To assess the cellulase production in detail, basal salt medium (BSM) was supplemented with synthetic [carboxymethyl cellulose (CMC), glucose, sucrose, dextrose, l...

Proteases or peptidases constitute the largest group of enzymes in bio-industry with a long array of uses. They play an invincible role in industrial biotechnology, especially in detergent, food and pharmaceutical arena. This focused review encompasses an overview on alkaline proteases, mainly of microbial sources in a handy module. Following an in...

In this study we describe a novel dark-green strain of Trichoderma viride exhibiting complete ensemble of cellulase, hemicellulase and ligninase activities on specific plate assays. To assess the cellulase produc-tion in detail, basal salt medium (BSM) was fortified with synthetic (carboxymethyl cellulose (CMC), glu-cose, sucrose, dextrose, lactose...

This unique study describes how Aspergillus japonicus, Penicillium brocae and Purpureocillium lilacinum, three novel isolates of our laboratory from heavily plastics-contaminated soil completely utilized the plasticizer di(2-ethylhexyl)phthalate (DEHP) bound to PVC blood storage bags (BB) in simple basal salt medium (BSM) by static submerged growth...

Phthalates are esters of phthalic acid (PAE) and are mainly used as plasticizers, i.e., substances added to plastics to increase their flexibility, transparency, durability and longevity. There exists many phthalates of varying chain lengths. PAE with longer alkyl chains i.e., di-(2-ethyl hexyl) phthalate (DEHP) and di-octyl phthalate (DOP) are poo...

Towards a process development for the bioremediation of phthalic acid esters (PAE) blended in various plastics, we explored the type of PAE in commercially available PVC blood storage bags (BB), its solvent-and temperature-dependent migrations and coefficient of diffusion (D) behaviors at static condition by solvent extraction method. The PAE [abou...