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Publications
Publications (5)
Endangered Southern Resident killer whales (Orcinus orca) are fish-eaters that preferentially prey on adult Chinook salmon (Oncorhynchus tshawytscha). Despite being salmon specialists, individuals from all three killer whale pods (J, K, L) have been observed harassing and killing porpoises (family Phocoenidae) without consuming them. Retrospectivel...
Blood crossmatching is necessary to determine transfusion compatibility between individuals, especially for species for which blood groups have not yet been defined, such as the killer whale (Orcinus orca). This study evaluated methodology for crossmatching in killer whales from a managed care population using individuals of known line-ages. Twenty...
In the United States, Canada, and Europe harbor seal (Phoca vitulina) pups are commonly rehabilitated after stranding and then released. Size at release is likely important to post-release survival; however, data have not been compiled to track the body condition of rehabilitated harbor seals at release across the U.S. To better understand spatiote...
Cryptococcus gattii is a fungal pathogen that primarily affects the respiratory and nervous systems of humans and other animals. C. gattii emerged in temperate North America in 1999 as a multispecies outbreak of cryptococcosis in British Columbia (Canada) and Washington State and Oregon (USA), affecting humans, domestic animals, and wildlife. Here...
In savannas across the planet, encroaching woody plants are altering ecosystem functions and reshaping communities. Seed predation by rodents may serve to slow the encroachment of woody plants in grasslands and savannas. Our goals for this study were to determine if rodents in an African savanna selectively removed seeds of an encroaching plant and...
Questions
Question (1)
I am validating an ELISA measuring A1c (also called glycated hemoglobin; target for humans) for use in another species. It is a direct sandwich assay. The standard and my sample (whole blood) were both serially diluted twofold.
I am wondering if anybody can help diagnose what I am seeing in my parallelism graph. As you can see in my graph (attached), the curves are near inverse of each other (linearity R2 = -0.91). Since it is a direct assay, I would of course expect this correlation to be positive.
I have gone over my plate map and how I set up my dilution rack a hundred times in my head, and I am quite certain that I did load my plate in the correct sequence as my plate map - ie, having both standard and sample increase in concentration down their respective column. Is there any other possibility (besides me incorrectly loading my plate according to my map) that could cause this "perfectly incorrect" parallelism correlation?
I would love to be proved wrong and find out that it was indeed my error in loading - but I'm pretty sure I loaded it correctly...so I just wanted to check if there was any biological answer to this (matrix effect?)
I plan to run another plate tomorrow to check- but just wanted to ask.
Thank you!