Sarah M Strycharz

Sarah M Strycharz
United States Naval Research Laboratory | NRL · Center for Biomolecular Science and Engineering

PhD

About

93
Publications
8,163
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,577
Citations
Citations since 2016
56 Research Items
1799 Citations
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
2016201720182019202020212022050100150200250
Introduction
I work in the area of microbial electrochemistry or electromicrobiology. I have studied anodic reactions with the model electricigen Geobacter and helped develop a model for electron transport in Geobacter biofilms. Currently, my work focuses on marine biocathode biofilms consisting of microbial consortia that are self-assembling and self-sustaining for electron consumption.
Additional affiliations
June 2011 - present
United States Naval Research Laboratory
Position
  • Research Biologist
October 2006 - January 2009
University of Massachusetts Amherst
Position
  • PostDoc Position
January 2002 - September 2006
University of South Carolina
Position
  • PhD Student

Publications

Publications (93)
Article
Full-text available
Chemolithotrophic bacteria capable of extracellular electron uptake to drive energy metabolism and CO 2 fixation are known as electroautotrophs. The recently described order Tenderiales contains the uncultivated electroautotroph “ Ca. Tenderia electrophaga.” The “ Ca. Tenderia electrophaga” genome contains genes proposed to make up a previously und...
Preprint
Engineered electroactive bacteria have potential applications ranging from sensing to biosynthesis. In order to advance the use of engineered electroactive bacteria, it is important to demonstrate functional expression of electron transfer modules in chassis adapted to operationally relevant conditions, such as non-freshwater environments. Here, we...
Preprint
Full-text available
Electromicrobiology can be used to understand extracellular electron uptake in previously undescribed chemolithotrophs. Enrichment and characterization of the uncultivated electroautotroph “ Candidatus Tenderia electrophaga” using electromicrobiology led to the designation of the order Tenderiales . Representative Tenderiales metagenome assembled g...
Chapter
Living conductive biofilms are biomaterials comprised of bacterial cells electrically connected to each other and to electrodes across multiple length scales. The properties of living conductive biofilms are due to the ability of some bacteria to form a network of charge carrying proteins across the cell membrane and through the biofilm matrix in a...
Article
Full-text available
Bin/Amphiphysin/RVS (BAR) domain proteins belong to a superfamily of coiled-coil proteins influencing membrane curvature in eukaryotes and are associated with vesicle biogenesis, vesicle-mediated protein trafficking, and intracellular signaling. Here we report a bacterial protein with BAR domain-like activity, BdpA, from Shewanella oneidensis MR-1,...
Article
Electroactive bacteria produce or consume electrical current by moving electrons to and from extracellular acceptors and donors. This specialized process, known as extracellular electron transfer, relies on pathways composed of redox active proteins and biomolecules and has enabled technologies ranging from harvesting energy on the sea floor, to ch...
Article
Electroactive bacteria are living catalysts, mediating energy-generating reactions at anodes or energy storage reactions at cathodes via extracellular electron transfer (EET). The Cathode-ANode (CANode) biofilm community was recently shown to facilitate both reactions, however, the identity of the primary constituents and underlying molecular mecha...
Article
Biofilms growing aerobically on conductive substrates are often correlated with a positive, sustained shift in their redox potential. This phenomenon has a beneficial impact on microbial fuel cells by increasing their overall power output but can be detrimental when occurring on stainless steel by enhancing corrosion. The biological mechanism behin...
Article
Full-text available
A strain of Geobacter sulfurreducens, an organism capable of respiring solid extracellular substrates, lacking four out of five outer membrane cytochrome complexes (strain extABCD⁺) grows faster and produces greater current density compared to wild type grown under identical conditions. To understand cellular and biofilm modifications altered in ex...
Article
Full-text available
Marinobacter spp. are opportunitrophs with a broad metabolic range including interactions with metals and electrodes. Marinobacter atlanticus strain CP1 was previously isolated from a cathode biofilm microbial community enriched from a sediment microbial fuel cell. Like other Marinobacter spp., M. atlanticus generates small amounts of electrical cu...
Article
Full-text available
Abstract The Tri-Service Microbiome Consortium (TSMC) was founded to enhance collaboration, coordination, and communication of microbiome research among U.S. Department of Defense (DoD) organizations and to facilitate resource, material and information sharing among consortium members. The 2019 annual symposium was held 22–24 October 2019 at Wright...
Article
Nature has developed myriad ways for organisms to interact with their environment using light and electronic signals. Optical and electronic properties can be observed macroscopically by measuring light emission or electrical current, but are conferred at the molecular level by the arrangement of small biological molecules, specifically proteins. H...
Article
Electrogenic microbes utilize anodes as metabolic electron acceptors producing electrical current. Recently, Beuth et al. reported that, when grown on copper anodes in the presence of sulfate, they can induce the precipitation of copper sulfide, which becomes incorporated into the growing biofilms, increasing their conductivity and net electrical o...
Article
Bacterial extracellular electron transfer (EET) is envisioned for use in applied biotechnologies, necessitating electrochemical characterization of natural and engineered electroactive biofilms under conditions similar to the target application, including small-scale biosensing or biosynthesis platforms, which is often distinct from standard 100 mL...
Article
Microbes that form biofilms on electrodes and generate electrical current responses could be integrated into devices to perform sensing, conduct signals, or act as living microprocessors. A challenge in working with these species is the ability to visualize biofilm formation and protein expression in real-time while also measuring current, which is...
Preprint
Full-text available
Bin/Amphiphysin/RVS (BAR) domain proteins belong to a ubiquitous superfamily of coiled-coil proteins that influence membrane curvature in eukaryotes and are associated with vesicle biogenesis, vesicle-mediated protein trafficking, and intracellular signaling. BAR domain proteins have not been identified in bacteria, despite certain organisms displa...
Article
Bin/Amphiphysin/RVS (BAR) domain proteins belong to a ubiquitous superfamily of coiled-coil proteins that influence membrane curvature in eukaryotes and are associated with vesicle biogenesis, vesicle-mediated protein trafficking, and intracellular signaling. BAR domain proteins have not been identified in bacteria, despite certain organisms displa...
Article
Bacterial microcompartment (BMC) shells are modular, selectively-permeable, nanoscale protein shells that self-assemble from hexagonal and pentagonal building blocks in vivo or in vitro. Natural and engineered BMC shells co-localize and concentrate catalysts and metabolites in their lumen, increasing reaction kinetics. Here, we describe the design...
Article
Full-text available
Organism engineering requires the selection of an appropriate chassis, editing its genome, combining traits from different source species, and controlling genes with synthetic circuits. When a strain is needed for a new target objective, for example to produce a chemical-of-need, the best strains, genes, techniques, software, and expertise may be d...
Article
Full-text available
Here, we present the complete genome sequence of Leisingera aquamixtae R2C4, isolated from the electroautotrophic microbial consortium biocathode MCL ( Marinobacter - Chromatiaceae - Labrenzia ). As an isolate of a current-producing system, the genome sequence of L. aquamixtae will yield insights regarding electrode-associated microorganisms and co...
Article
Full-text available
The marine heterotroph, Marinobacter atlanticus strain CP1, was recently isolated from the electroautotrophic Biocathode MCL community, named for the three most abundant members: Marinobacter, an uncharacterized member of the Chromatiaceae, and Labrenzia. Biocathode MCL catalyzes the production of cathodic current coupled to carbon fixation through...
Article
Natural living conductive biofilms transport electrons between electrodes and cells, as well as among cells fixed within the film, catalyzing an array of reactions from acetate oxidation to CO2 reduction. Synthetic biology offers tools to modify or improve electron transport through biofilms, creating a new class of engineered living conductive mat...
Article
Full-text available
Here, we report on the development of a genetic system for Marinobacter sp. strain CP1, previously isolated from the Biocathode MCL community and shown to oxidize iron and grow as a cathodic biofilm. Sequence analysis of the small and large subunits of the 16S rRNA gene of CP1, as well as comparison of select conserved proteins, indicate that it is...
Article
Here we demonstrate the method of electrochemical gating used to characterize electrical conductivity of electrode-grown microbial biofilms under physiologically relevant conditions.¹ These measurements are performed on living biofilms in aqueous medium using source and drain electrodes patterned on a glass surface in a specialized configuration re...
Article
The ability of certain microorganisms to live in a multi-cell thick, electrode-grown biofilm by utilizing the electrode as a metabolic electron acceptor or donor requires electron transfer across cell membranes, through the biofilm, and across the biofilm/electrode interface. Even for the most studied system, anode-grown Geobacter sulfurreducens, t...
Article
Full-text available
Here we report enrichment from a marine-derived inoculum of a non-photosynthetic electroactive biofilm that is capable of both consuming electricity (electrotrophy) and producing electricity (electrogenesis) from a single electrode. By alternating the electrode potential between –0.4 VSHE and 0.0 VSHE every 10 min, alternating anodic and cathodic c...
Article
Full-text available
Biocathode microbial communities are proposed to catalyse a range of useful reactions. Unlike bioanodes, model biocathode organisms have not yet been successfully cultivated in isolation highlighting the need for culture-independent approaches to characterization. Biocathode MCL (Marinobacter, Chromatiaceae, Labrenzia) is a microbial community prop...
Data
Fig. S2 (Krona plot, download file before viewing in browser). Interactive Krona plots depicting relative abundance of 20 resolved dominant Ray‐MetaBAT case 1b bin genomes to each other classified by AMPHORA2 (i.e. Sphingomonadaceae should be Kordiimonadaceae as noted in the main text).
Data
Data S1. Supplemental Materials and Methods. Table S1. Biocathode MCL strain IDs and associated metrics. Table S2. Biocathode MCL metagenomic assembly metrics for both individual and pooled bioelectrochemical system (BES) sample reads. Table S3. Metagenomic binning and quality checks. Table S4. Relative abundance for all 7 replicate bioelectroc...
Data
Fig. S4a‐b (Krona plot, download file before viewing in browser). Interactive Krona plots depicting relative abundance predicted by 16S rRNA gene amplicon sequencing of each hypervariable region for eight replicate bioelectrochemical systems (BES) using OTUs generated by CD‐HIT with RDP classifier (a) or mothur (b).
Data
Fig. S3a‐c (Krona plot, download file before viewing in browser). Interactive Krona plots depicting relative abundance of predicted taxa at the genus, family, and order levels resolved using Metaphyler (a), Kraken (b), and blastn (c).
Article
Full-text available
Bacteria that directly use electrodes as metabolic electron donors (biocathodes) have been proposed for applications ranging from microbial electrosynthesis to advanced bioelectronics for cellular communication with machines. However, just as we understand very little about oxidation of analogous natural insoluble electron donors, such as iron oxid...
Article
Bacterial electroactive biofilms attract researchers’ attention due to their ability for generation of electric current when electrons travel for very long distance, up to several tens microns in the course of cell metabolic activity. It was demonstrated that this electron transfer (ET) ability correlates with the accumulation in the BF huge amount...
Article
Microbial electrosynthesis (ME) seeks to use electroautotrophy (the reduction of CO2 by microbial electrode catalysts) to generate useful multi-carbon compounds. It combines the utility of electrosynthesis with the durability of microorganisms and potential to engineer microbial metabolic processes. Central to achieving efficient ME is understandin...
Preprint
Full-text available
Biocathodes provide a stable electron source to drive reduction reactions in electrotrophic microbial electrochemical systems. Electroautotrophic biocathode communities may be more robust than monocultures in environmentally relevant settings, but some members are not easily cultivated outside of the electrode environment. We previously used metage...
Article
Full-text available
Importance: The residual heating of living organisms in the microwave region of the electromagnetic spectrum has complicated the identification of radiation-only effects using microorganisms for 50 years. A majority of the previous experiments used either mature cells or short exposure times with low-energy high frequency radiation. Using global d...
Article
Full-text available
Here, we present the complete genome sequence of Labrenzia sp. strain CP4, isolated from an electricity-consuming marine biocathode biofilm. Labrenzia sp. strain CP4 consists of a circular 5.2 Mbp chromosome and an 88 Kbp plasmid.
Article
When grown on the surface of anode electrode, Geobacter sulfurreducens forms a multi-cell thick biofilm in which all cells appear to couple oxidation of acetate with electron transport to the anode which serves as the terminal metabolic electron acceptor. Just how electrons are transported through such a biofilm from cells to the underlying anode s...
Article
Biocathode MCL ( Marinobacter, Chromatiaceae, Labrenzia ) is a cathode associated microbial consortium enriched from seawater.(1-4) It is extremely durable, having been maintained in the laboratory for at least 6 years with minimal care, and acquires energy by coupling electron uptake from graphite or gold electrodes with reduction of O 2 , directi...
Article
Full-text available
Biocathode communities are of interest for a variety of applications, including electrosynthesis, bioremediation, and biosensors, yet much remains to be understood about the biological processes that occur to enable these communities to grow. One major difficulty in understanding these communities is that the critical autotrophic organisms are diff...
Article
Microorganisms that respire electrodes may be exploited for biotechnology applications if key pathways for extracellular electron transfer (EET) can be identified and manipulated through bioengineering. To determine whether expression of proposed Biocathode-MCL EET proteins are changed by modulating electrode potential without disrupting the relati...
Article
Full-text available
Microbial biofilms grown utilizing electrodes as metabolic electron acceptors or donors are a new class of biomaterials with distinct electronic properties. Here we report that electron transport through living electrode-grown Geobacter sulfurreducens biofilms is a thermally activated process with incoherent redox conductivity. The temperature depe...
Article
Full-text available
Marinobacter sp. CP1 was isolated from a self-regenerating and self-sustaining biocathode biofilm that can fix CO2 and generate electric current. We present the complete genome sequence of this strain, which consists of a circular 4.8-Mbp chromosome, to understand the mechanism of extracellular electron transfer in a microbial consortium.
Chapter
In this chapter, a proposed mechanism for long-distance electron transport that occurs over distances that can exceed 20 μm within electrochemically active biofilms comprised of Geobacter sulfurreducens wild-type strain DL-1 is described. According to this mechanism, referred to as redox conduction, long-distance electron transport results from seq...
Article
Microorganisms that respire electrodes may be exploited for biotechnology applications if key pathways for extracellular electron transfer (EET) can be identified and manipulated through bioengineering. To determine whether expression of proposed Biocathode-MCL EET proteins are changed by modulating electrode potential without disrupting the relati...
Article
Biocathode extracellular electron transfer (EET) may be exploited for biotechnology applications, including microbially mediated O2 reduction in microbial fuel cells and microbial electrosynthesis. However, biocathode mechanistic studies needed to improve or engineer functionality have been limited to a few select species that form sparse, homogene...
Article
Geobacter sulfurreducens biofilms were grown to early exponential phase (i.e. the point at which the catalytic current first begins to increase) on interdigitated microelectrode arrays (IDAs), resulting in the formation of sparse cell clusters surrounded by extracellular polymeric substance (EPS). Continuous domains of EPS (defined here to include...
Article
Full-text available
The pairing of a nitrate reducing microbial biocathode with an organic matter oxidizing microbial bioanode represents a potential high value wastewater treatment methodology. While such bioanodes are relatively optimized, such biocathodes suffer from relatively low current densities and low operating potentials. Here we present enrichment and chara...
Article
Full-text available
Atomic force microscopy and confocal resonance Raman microscopy (CRRM) of single-cells were used to study the transition of anode-grown Geobacter sulfurreducens biofilms from lag phase (initial period of low current) to exponential phase (subsequent period of rapidly increasing current). Results reveal that lag phase biofilms consist of lone cells...
Article
When grown on the surface of an anode electrode, Geobacter sulfurreducens forms a multi-cell thick biofilm in which all cells appear to couple the oxidation of acetate with electron transport to the anode, which serves as the terminal metabolic electron acceptor. Just how electrons are transported through such a biofilm from cells to the underlying...
Article
Full-text available
Microbial solar cells (MSCs) are microbial fuel cells (MFCs) that generate their own oxidant and/or fuel through photosynthetic reactions. Here, we present electrochemical analyses and biofilm 16S ribosomal-RNA gene profiling of biocathodes of sediment/seawater-based MSCs inoculated from the biocathode of a previously described sediment/seawater-ba...