Sarah Nietzer

Sarah Nietzer
University of Wuerzburg | JMU · Chair of Tissue Engineering and Regenerative Medicine

Dr. rer. nat.

About

46
Publications
7,482
Reads
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397
Citations
Citations since 2017
6 Research Items
334 Citations
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2017201820192020202120222023010203040506070
2017201820192020202120222023010203040506070
2017201820192020202120222023010203040506070

Publications

Publications (46)
Article
Epithelial-to-mesenchymal transition (EMT) is discussed to be centrally involved in invasion, stemness, and drug resistance. Experimental models to evaluate this process in its biological complexity are limited. To shed light on EMT impact and test drug response more reliably, we use a lung tumor test system based on a decellularized intestinal mat...
Article
Precision medicine has changed thinking in cancer therapy, highlighting a better understanding of the individual clinical interventions. But what role do the drivers and pathways identified from pan-cancer genome analysis play in the tumor? In this letter, we will highlight the importance of in silico modeling in precision medicine. In the current...
Article
Curcumin (CUR)is a natural extract from the plant Curcuma longa and part of turmeric, a spice and herbal remedy in traditional medicine. Thousands of papers claim a plethora of health benefits by CUR, but a growing number of reports and contributions caution that many experimental data may be artifacts or outright deny any suitability of CUR due to...
Article
Full-text available
Patient‐tailored therapy based on tumor drivers is promising for lung cancer treatment. For this we combined in vitro tissue models with in silico analyses. Using individual cell lines with specific mutations we demonstrate a generic and rapid stratification pipeline for targeted tumor therapy. We improve in vitro models of tissue‐conditions by a b...
Article
Full-text available
MicroRNAs are well-known strong RNA regulators modulating whole functional units in complex signaling networks. Regarding clinical application, they have potential as biomarkers for prognosis, diagnosis, and therapy. In this review, we focus on two microRNAs centrally involved in lung cancer progression. MicroRNA-21 promotes and microRNA-34 inhibit...
Article
Full-text available
Development of predictable in vitro tumor models is a challenging task due to the enormous complexity of tumors in vivo. The closer the resemblance of these models to human tumor characteristics, the more suitable they are for drug-development and –testing. In the present study, we generated a complex 3D lung tumor test system based on acellular ra...
Data
Evaluation of extracellular matrix (ECM) components in decellularized lung scaffolds. (A) Acellular lungs generated by SDS- and CHAPS-protocols tend to contain a lower amount of collagen per mg dry tissue than the scaffolds generated with Triton-SDC or H2O-SDC. While these exhibited significantly higher collagen content than native rat lungs (p = 0...
Data
Evaluation of decellularization protocols. (A) The percentage of airspace in the decellularized matrices was compared to native lungs to quantify the structural preservation for different decellularization protocols. While scaffolds generated using Triton-SDC and H2O-SDC exhibited similar values to native tissue, the CHAPS- and SDS scaffolds showed...
Data
Histologic assessment of extracellular matrix components in the decellularized lung matrices. All scaffolds showed similar and global retention of the basement membrane components collagen IV and fibronectin. In contrast, a reduced intensity for collagen I and elastin was observed in scaffolds generated with the SDS- and CHAPS– protocols. This was...
Data
Schematic illustration of the different decellularization protocols used in this study. Each protocol utilizes a different perfusion pressure, volume or duration, respectively. Noteworthy, the SDS- and CHAPS-protocols use only the vascular system as the route of application. Protocols employing Triton-SDC and H2O-SDC apply decellularization solutio...
Data
Separated and sectioned rat lung for analysis. Decellularized and native lung tissue was processed as depicted here to allow different analyses with one scaffold. Except for ultrastructural studies, each analysis was performed with tissue pieces of each part of the lung. The specific use of the single tissue pieces is listed in S1 Table. (TIF)
Data
Antibodies used for immunohistochemical staining and immunofluorescence. (PDF)
Data
Analyses performed with different tissue pieces of the lung. (PDF)
Article
Aim: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. Materials & methods: We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling mainten...
Article
Full-text available
Tumor models based on cancer cell lines cultured two-dimensionally (2D) on plastic lack histological complexity and functionality compared to the native microenvironment. Xenogenic mouse tumor models display higher complexity but often do not predict human drug responses accurately due to species-specific differences. We present here a three-dimens...
Article
Cancer is one of the leading causes of death worldwide. Current therapeutic strategies are predominantly developed in 2D culture systems, which inadequately reflect physiological conditions in vivo. Biological 3D matrices provide cells an environment in which cells can self-organize, allowing the study of tissue organization and cell differentiatio...
Article
Full-text available
In the present study, we combined an in vitro 3D lung tumor model with an in silico model to optimize predictions of drug response based on a specific mutational background. The model is generated on a decellularized porcine scaffold that reproduces tissue-specific characteristics regarding extracellular matrix composition and architecture includin...
Poster
Full-text available
The potential of preclinical tumor models to predict the outcome of substances in the clinic is limited. More advanced models that include features of living tissues and parts of the microenvironment are urgently needed. At the chair of “Tissue Engineering and Regenerative Medicine” (TERM) and the Translational Center “Regenerative therapies in onc...
Article
Zielsetzung: Das Verstandnis der Signalwege von Lungentumorgeweben ist die Voraussetzung fur die Entwicklung neuer und individualisierter Behandlungsstrategien. Methode: Tumorzellen zweier Zelllinien mit unterschiedlichem EGF-Rezeptor-Mutationsstatuts (A549 & HCC827) wurden auf einer auf der dezellularisierten, biologischen Tragerstruktur SISmuc au...
Data
Supplementary Figure S2TGFβ stimulation. Dynamic model simulation of different nodes of the signaling network (Figure 4A) using SQUAD. TGFβ‐receptor stimulation (initial value of TGFβ: 1.0), results in EMT signaling (0.9, nearly maximal), the activity of several other network nodes is shown in the cell‐type specific context and activation state of...
Data
Supplementary Figure S3Gefitinib effects on HCC827 in the 3D tumor tissue model. In 3D conditions the EGFR activation is reduced and apoptosis is induced in HCC827 by gefitinib. Immunohistochemical stainings of 3D tumor tissue models show weak signals for the EGFR in A549 (A, B) with no obvious phosphorylation (E, F). In HCC827 the EGFR could be cl...
Data
Supplementary Figure S1In silico modeling of gefitinib response in A549 cells bearing a Kras mutation. Dynamic model simulation of different nodes (assuming constantly hyperactive Kras at 0.4) of the signaling network (Figure 4A) using SQUAD. (A) An activating signal of the EGFR (value: 0.3; EGF approximately 0.4, not strong, wild type) evokes a mo...
Article
Full-text available
For the development of new treatment strategies against cancer, understanding signaling networks and their changes upon drug response is a promising approach to identify new drug targets and biomarker profiles. Pre-requisites are tumor models with multiple read-out options that accurately reflect the clinical situation. Tissue engineering technolog...
Conference Paper
Full-text available
Robust and reliable dynamic bioreactors for long term maintenance of various tissues at milliliter-scale on the basis of a biological, vascularized matrix (BioVaSc®) have been developed at the Fraunhofer IGB in Stuttgart, Germany. As an intestinal in vitro equivalent, seeding of the matrix with CaCo-2 cells yielded in the self-assembly of a microen...
Article
Full-text available
Cancer is one of the leading causes of death worldwide. Current therapeutic strategies are predominantly developed in 2D culture systems, which inadequately reflect physiological conditions in vivo. Biological 3D matrices provide cells an environment in which cells can self-organize, allowing the study of tissue organization and cell differentiatio...
Article
Cancer is one of the leading causes of death world-wide. For drug screening and development, appropriate tumor models are needed that reflect the situation in the patient robustly and accurately. Next to the identification of new compounds, cancer research contributes to the understanding of complex tumor biology. This enables targeted and individu...
Article
Low expression of the human serotonin transporter (5-HTT) gene presumably interacts with stressful life events enhancing susceptibility for affective disorders. 5-Htt knockout (KO) mice display an anxious phenotype, and behavioural differences compared to wild-type (WT) mice are exacerbated after repeated loser experience in a resident-intruder str...
Article
Serotonin (5-HT) is an important modulator of many physiological, behavioural and developmental processes and it plays an important role in stress coping reactions. Anxiety disorders and depression are stress-related disorders and they are associated with a malfunction of the 5-HT system, in which the 5-HT transporter (5-HTT) plays an important rol...
Article
Serotonin (5-HT) is an important modulator of many physiological, behavioural and developmental processes and it plays an important role in stress coping reactions. Anxiety disorders and depression are stress-related disorders and they are associated with malfunction of the serotonin system, in which the serotonin transporter (5-HTT) plays an impor...
Article
Depression and anxiety disorders are stress related disorders and they are associated with disturbances in the serotonergic system, in which the serotonin transporter (5-HTT) plays an important role. As 5-HTT-knockout mice represent an artificially hyperserotonergic environment, exhibit an anxious phenotype and exaggerated responses to stress they...
Article
Depression and anxiety disorders are stress related disorders and they are associated with disturbances in the serotonergic system, in which the serotonin transporter (5-HTT) plays an important role. As 5-HTT-knockout mice represent an artificially hyperserotonergic environment, exhibit an anxious phenotype and exaggerated responses to stress they...

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Projects

Project (1)
Project
Applying tissue engineering technologies to generate a human lung cancer model for applied research.