Sara Cuadros-Orellana

Sara Cuadros-Orellana
Universidad Católica del Maule | UCM · Forestry Sciences

PhD
Full Professor and Researcher

About

55
Publications
12,012
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
822
Citations
Introduction
Dr. Sara Cuadros Orellana is Full Professor at Universidad Católica del Maule, in Chile working in microbial ecology and evolutionary genomics, with a special interest in extreme environments, such as salt flats, acid drainages and hot springs.
Additional affiliations
September 2010 - January 2017
Fundação Oswaldo Cruz
Position
  • Researcher
Description
  • Vale-Fiocruz Genomics Center Project: Genomics for Biotechnology Solutions. This project involved the deep sequencing and functional analysis of microbial communities in a copper mine environment.
Education
February 1999 - November 2004
University of Campinas
Field of study
  • Food Science / Microbiology
February 1997 - December 1998
University of São Paulo
Field of study
  • Genetics
March 1993 - December 1996
University of São Paulo
Field of study
  • Biological Sciences

Publications

Publications (55)
Article
Full-text available
Food contamination with microorganisms is responsible for food spoilage, deterioration and changing organoleptic properties of foods. Besides, the growth of pathogenic microorganisms can provoke serious health problems if food is consumed. Innovative packaging, such as active packaging, is increasing rapidly in the food industry, especially in appl...
Article
Full-text available
p>The Acknowledgements section in the original version of this Article was incomplete. “This study has received funding from the European Union’s Horizon 2020 research and innovation program under Grant agreement no. 643476 (COMPARE), the World Health Organization, and The Novo Nordisk Foundation (NNF16OC0021856: Global Surveillance of Antimicrobia...
Article
Full-text available
Rock phosphate (RP) is a natural source of phosphorus for agriculture, with the advantage of lower cost and less impact on the environment when compared to synthetic fertilizers. However, the release of phosphorus (P) from RP occurs slowly, which may limit its short-term availability to crops. Hence, the use of P-solubilizing microorganisms to impr...
Article
Full-text available
The rapid development of megacities, and their growing connectedness across the world is becoming a distinct driver for emerging disease outbreaks. Early detection of unusual disease emergence and spread should therefore include such cities as part of risk-based surveillance. A catch-all metagenomic sequencing approach of urban sewage could potenti...
Article
Background Thermophilic bacilli in both aerobic or facultative anaerobic forms have been isolated for over a hundred years from different mesophilic or thermophilic environments as they are potential source of bioactive secondary metabolites. But the taxonomic resolution in the Bacillus genus at species or at strain levelis very challenging for the...
Article
Full-text available
The cost of cellulase enzymes is a main contributor to the operational cost of a biorefinery producing ethanol from lignocellulosic material. Therefore, onsite production of enzymes using low-value substrates might be an option to make a bio-based facility more economical, while improving environmental sustainability. Food manufacturing wastes (FMW...
Chapter
Soil salinity is a key environmental factor restraining the productivity of soil and crop plants. In different parts of the world, agricultural productivity is decreasing mostly because of drought and salinity increase. The situation may become worse for global warming in the future. A wide range of adaptation and mitigation strategies have been ad...
Article
Full-text available
Antimicrobial resistance (AMR) is a serious threat to global public health, but obtaining representative data on AMR for healthy human populations is difficult. Here, we use metagenomic analysis of untreated sewage to characterize the bacterial resistome from 79 sites in 60 countries. We find systematic differences in abundance and diversity of AMR...
Article
Full-text available
In this work, a new electrochemical biosensor for DNA detection of bacterial meningitis is proposed. The system is based on specific DNA fragments from the Neisseria meningitidis genome as a probe incorporated on graphite electrodes modified with poly(4-aminophenol). Detection of a complementary oligonucleotide sequence, a specific 710-base pair am...
Article
The Streptococcus pneumoniae detection plays an important role in the diagnosis and monitoring of pneumococcal diseases. A genosensor based on graphite electrodes modified with polymer was developed for such detection. First, the poly(4-aminophenol) film was electrochemically deposited on a graphite electrode. Afterward, an S. pneumoniae-specific o...
Article
Full-text available
Acid mine drainage (AMD) is characterized by an acid and metal-rich runoff that originates from mining systems. Despite having been studied for many decades, much remains unknown about the microbial community dynamics in AMD sites, especially during their early development, when the acidity is moderate. Here, we describe draft genome assemblies fro...
Article
Full-text available
Phosphate fertilization is a common practice in agriculture worldwide, and several commercial products are widely used. Triple superphosphate (TSP) is an excellent soluble phosphorus (P) source. However, its high cost of production makes the long-term use of crude rock phosphate (RP) a more attractive alternative in developing countries, albeit its...
Data
Rarefaction curves of (A) bacteria and (C) fungi using QIIME software were calculated according observed OTU number in the treatment means. Control, without added P; RP, rock phosphate addition; TSP, addition of triple superphosphate. Furthermore, 0.99 of Good Coverage values from 10,000 reads for (B) bacteria and 20,000 for (D) fungi was observed...
Data
Community of mycorrhizal fungi detected in the soil fertilized with RP, TSP, and without the addition of P (control). Non-metric multidimensional scaling (NMDS) was based on the matrix of Jaccard and it was performed variance analysis of the distance matrix using the multivariate permutation test (Adonis).
Article
This paper describes the development of an immunosensor for the c-reactive protein (CRP), based on both direct and indirect electrochemical detections. The anti-CRP probe was grafted to the platform sensitized with poly(3-aminothiophenol) and the interaction of the CRP target with the immunosensor was carried either directly or indirectly, using 4-...
Article
Full-text available
A targeted amplicon-based metagenomics approach (metabarcoding) provides detailed access to the diversity of the mycobiome in any substrate in distinct environments on Earth. Fungi are the main decomposers of lignocellulosic woody debris in terrestrial forested ecosystems, contributing significantly to the global carbon cycle. The main objectives o...
Conference Paper
Full-text available
Sulfide mineral dissolution during acid mine drainage (AMD) formation creates an environment containing a notable variety of adaptive genes. We explored the distribution and diversity of metabolic pathways in an AMD site in the Brazilian Amazon, to better understand the mechanisms by which bacteria tolerate the acid/toxic conditions in this tropica...
Poster
Full-text available
We investigated the population history of Hydrotalea sp. based on their CRISPR repertoire. Six SAGs of Chitinophagaceae were partially reconstructed. Phylogenetic and ANI analyses suggest that all SAGs belong to Hydrotalea sp. They form two clusters representing different strains and cells inside the same cluster are clones over their genome. SAGS...
Article
Full-text available
Here, we present the draft genome sequence of the type strain of “ Acidibacillus ferrooxidans ,” a mesophilic, heterotrophic, and acidophilic bacterium that was isolated from mine spoilage subjected to accelerated weathering in humidity cell tests carried out by the former U.S. Bureau of Mines in Salt Lake City, UT.
Article
Full-text available
We report the draft genome sequence of Hydrotalea flava CCUG 51397 T , the type strain of the genus Hydrotalea (family Chitinophagaceae ), isolated from water samples in southern Sweden.
Article
Full-text available
Author Summary The potential interaction between ZIKA Virus (ZIKV) infection and infant brain defects still has no explanatory mechanism. The mechanism of action for several other viruses have been described, and are often related to the control or production of sncRNA (small noncoding RNA) molecules, which regulate the expression of several human...
Data
Tab-delimited text file containing the miRNA identifiers from human, with their respective Target region in the ZIKV Genomes, Total Score, Total Energy, Maximum Score per alignment, Maximum Energy per alignment, Strand, Length: miRNA, Length: target and Alignment positions. (TXT)
Data
Phylogenetic analysis of only the predicted miRNAs encoded by each ZIKV strain used in this study. The size of the full circles on the branches means percentage of bootstrap (100 replicates). (PDF)
Data
Tab-delimited text file containing the miRNA identifiers from ZIKV with their respective Target Ensembl Transcript, Total Score, Total Energy, Maximum Score per alignment, Maximum Energy per alignment, Strand, Length: miRNA, Length: target and Alignment positions. (TXT)
Data
FASTA file containing the mature sequences of the nine ZIKV encoded miRNAs. (FASTA)
Data
FASTA file containing the precursor sequences of the nine ZIKV encoded miRNAs. (FASTA)
Article
Full-text available
Here, we report the draft genome sequence of “ Acidibacillus ferrooxidans ” strain ITV01, a ferrous iron- and sulfide-mineral-oxidizing, obligate heterotrophic, and acidophilic bacterium affiliated with the phylum Firmicutes . Strain ITV01 was isolated from neutral drainage from a low-grade chalcopyrite from a mine in northern Brazil.
Article
Full-text available
In this work, three polymeric films derived from hydroxyphenylacetic acid isomers were electropolymerized onto the surfaces of graphite electrodes through cyclic voltammetry. Analysis of electrochemical behaviors and electrical properties of the three polymeric films were investigated and their probably structures were explored by molecular modelin...
Article
Full-text available
The aim of this study was to analyze the physicochemical properties and antifungal activities of the red propolis samples from Sergipe, Brazil, and also evaluate their variability throughout the year. The characterization of the hydroalcoholic extract (HPE) of the red propolis samples was performed monthly from October 2009 to September 2010. The c...
Article
Full-text available
Abstract Melipona scutellaris is a Brazilian stingless bee species and a highly important native pollinator besides its use in rational rearing for honey production. In this study, we present the whole mitochondrial DNA sequence of M. scutellaris from a haploid male. The mitogenome has a size of 14,862 bp and harbors 13 protein-coding genes (PCGs),...
Article
Full-text available
Bees are manufacturers of relevant economical products and have a pollinator role fundamental to ecosystems. Traditionally, studies focused on the genus Melipona have been mostly based on behavioral, and social organization and ecological aspects. Only recently the evolutionary history of this genus has been assessed using molecular markers, includ...
Article
Full-text available
Denaturing high-performance liquid chromatography (DHPLC) has been described as a suitable method to study DNA polymorphisms. Here, cassava (Manihot esculenta Crantz) fermentation liquor was examined using DHPLC analysis to characterize the bacterial diversity during the fermentation process. GC-clamped amplicons corresponding to a variable region...
Article
Full-text available
Fungi are ubiquitous inhabitants of soil and aquatic environments, and they establish and maintain either parasitic or symbiotic relationships with animals and plants. They are major players in nutrient cycling, especially in organic matter decomposition, and they are major sources of biologically active substances. However, their full metabolic po...
Article
Full-text available
Fungi are ubiquitous inhabitants of soil and aquatic environments, and they establish and maintain either parasitic or symbiotic relationships with animals and plants. They are major players in nutrient cycling, especially in organic matter decomposition, and they are major sources of biologically active substances. However, their full metabolic po...
Conference Paper
It is well established that aromatic compounds can be readily degraded in aerobic environments within soils, sediments and waters with salinities up to and including that of seawater. However, little is known about their metabolism in hypersaline environments. There is a growing interest in the development and optimization of bioremediation process...
Article
Full-text available
The chloroform extract of Cladonia substellata Vainio was assayed against larvae of Aedes ae- gypti, the mosquito vector of Dengue fever and Artemia salina. The extract was tested at concentrations ranging from 1 to 15 ppm in an aqueous medium for 24 h. LC50 and LC90 were evaluated. Since the chlo- roform extract proved to be lethal for third to fo...
Article
Full-text available
The variability in genome content among closely related strains of prokaryotes has been one of the most remarkable discoveries of genomics. One way to approach the description of this so-called pan-genome is to compare one reference strain genome with metagenomic sequences from the environment. We have applied this approach to one extreme aquatic h...
Article
Full-text available
It is widely known that microorganisms have the ability to metabolize xenobiotics in the environment, and their study has received much attention due to the environmental persistence and toxicity of these compounds. Several works published in the field have concluded that a wide variety of microorganisms in pure and mixed culture under both aerobic...
Article
Full-text available
Benzoic acid and its hydroxylated derivatives are naturally occurring compounds that are also released into the environment in large amounts through industrial wastewater, automobile exhaust and tobacco smoke. Even though these compounds are readily degraded under mild environmental conditions, little is known about their metabolism in extreme envi...
Article
Full-text available
Undecanoic acid (UDA) is a fatty acid that has been used in the treatment of dermatophytoses in humans. Formulations containing UDA have been used in the treatment of superficial mycoses, such as athlete’s foot (tinea pedis) and keratomycoses (Chretien et al., 1980). It was reported that high concentrations of UDA inhibit the ubiquitous dermatophyt...
Article
Full-text available
In this report, undecanoic acid is shown to affect enzymatic activity in the fungus Aspergillus nidulans. In a UDA-resistant mutant strain, the extracellular activity of lipases on p-nitrophenol decreased approximately 40% compared to the wild-type strain. The lipase activity on Tween 80 was completely inhibited by UDA. Alterations were observed in...
Article
Full-text available
It is well established that aromatic compounds can be readily degraded in aerobic environments within soils, sediments and waters with salinities up to and including that of seawater. However, little is known about their metabolism in hypersaline environments. There is a growing interest in the development and optimization of bioremediation process...
Conference Paper
Full-text available
Metagenomics aims at studying the genetic material recovered directly from environmental samples, enabling the identification of taxonomic groups and gene sequences of unculturable or difficult to culture microorganisms. New DNA sequencing methodologies mostly enabled deep metagenomics research. The level of untapped biological diversity has been d...

Network

Cited By

Projects

Projects (6)
Project
The Chilean Single Cell Sequencing (SCS) platform is funded by the Chilean National Research and Development Agency (ANID), and consists of a BD FACSMelody™ Cell Sorter microfluidic unit that performs fluorescence-assisted cell sorting, and an Illumina MiSeq System massively parallel sequencing unit that enables reading of genetic information derived from sorted cells. The cell sorting platform is composed of 4 lasers and allows classifying individual cells or cell populations derived from complex samples based on 9 different colors. The sequencing platform generates up to 15 billion DNA bases in a single run. Single cell sequencing is applicable to all areas of knowledge that seek to understand the functioning of individual cells and/or intra-population genetic variations. Contact information: scuadros@ucm.cl
Project
This project seeks to incorporate antifungal natural extracts into fruit export packaging. The goal is to reduce the occurrence of fruit decay, which are caused by fungi such as Botrytis cinerea. The project is a partnership between the university, small producers, a packaging manufacturer and several fruit exporting companies in Chile.