Sankar Raju Narayanasamy

• Doctor of Philosophy
• Lawrence Berkeley National Laboratory

Compared with batch and vapor diffusion methods, counter diffusion can generate larger and higher-quality protein crystals yielding improved diffraction data and higher-resolution structures. Typically, counter-diffusion experiments are conducted in elongated chambers, such as glass capillaries, and the crystals are either directly measured in the...

Serial crystallography at large facilities, such as x-ray free-electron lasers and synchrotrons, evolved as a powerful method for the high-resolution structural investigation of proteins that are critical for human health, thus advancing drug discovery and novel therapies. However, a critical barrier to successful serial crystallography experiments...

Cellulose, a sustainable source of fermentable glucose and nanomaterials, exists as highly-ordered, solvent-inaccessible fibrils held by intra- and intermolecular hydrogen bonds. Cellulose hydrolysis rates and extents are limited by the...

The development of x-ray free electron laser (XFEL) light sources and serial crystallography methodologies has led to a revolution in protein crystallography, enabling the determination of previously unobtainable protein structures and near-atomic resolution of otherwise poorly diffracting protein crystals. However, to utilize XFEL sources efficien...

Over the past two decades, serial X-ray crystallography has enabled the structure determination of a wide range of proteins. With the advent of X-ray free-electron lasers (XFELs), ever-smaller crystals have yielded high-resolution diffraction and structure determination. A crucial need to continue advancement is the efficient delivery of fragile an...

Well-defined aerosols pave the way for versatile basic and applied research. Here, we demonstrate a unique whipping instability that generates from a high-aspect-ratio microfluidic device resulting in a unique steady-state gas-focused whipping jet (WJ) without any need for electrification. This WJ device emanates a multi-monodisperse whipping spray...

High-density microfluidics is becoming an important experimental platform for studying complex biological systems such as synthetic gene regulatory networks, molecular biocomputating of engineered cells, distributing rapid point-of-care diagnosis, and monitoring pathological environment. Imaging transient bio-chemical reactions happening in these s...

Synchrotron infrared hyperspectral microscopy is a label-free and non-invasive technique well suited for imaging of chemical events in situ. It can track the spatial and temporal distributions of molecules of interests in a specimen in its native state by the molecule's characteristic vibrational modes. Despite tremendous progress made in recent ye...

The research presented in this thesis primarily focuses on the dynamics of fluids of microfluidic liquid flat jet and the numerical methodology associated understanding of this complex thin sheet formation therein. The gas focussing is based on the gas dynamic virtual nozzle (GDVN) principle. This liquid sheet has eminated with a huge scope in the...

•An explanatory guide for the �eld and force function objects for post processing in OpenFOAM • A brief description of the library classi�cation depending on the inheritance of function objects. • Procedure to evoke Evoke the existing function objects which are present in the OpenFOAM library by default for post-processing and to control them on ru...

In modern society the awareness of disturbing noise has increased. Thus it is of importance to find the cause of noise generation and prevent the designs which produces them. In this report the wind-induced whistling noise from two sailboat mast profiles have been evaluated. It has previously been observed that one of the profiles generates a whist...

Numerical analysis of supersonic flows using Open FOAM, an open source CFD tool, is performed for double ramp model. Two-dimensional, unsteady, compressible, turbulent flow equations have been solved. Initially a grid independent study is performed for the model and deviations are studied and henceforth a suitable mesh with minimum deviations is co...