Sanjeev Kumar Mahto

Sanjeev Kumar Mahto
Indian Institute of Technology (Banaras Hindu University) Varanasi | BHU · School of Bio-Medical Engineering

PhD

About

75
Publications
8,992
Reads
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769
Citations
Additional affiliations
May 2014 - June 2021
Indian Institute of Technology (Banaras Hindu University) Varanasi
Position
  • Professor (Assistant)
September 2013 - April 2014
The University of Calgary
Position
  • PostDoc Position
December 2011 - September 2013
Technion - Israel Institute of Technology
Position
  • PostDoc Position

Publications

Publications (75)
Article
Bone tissue engineering is an emerging technology that has been developed in recent years to address bone abnormalities by repairing, regenerating and replacing damaged/injured tissues. In present work, we report the fabrication and characterization of porous luffa-based composite scaffolds composed of Luffa cylindrica (sponge gourd) powder (LC)/hy...
Article
Full-text available
Chronic elevation of sugar and oxidative stress generally results in development of advanced glycation end products (AGEs) in diabetic individuals. Accumulation of AGEs in an individual can give rise to the activation of several pathways that will ultimately lead to various complications. Such AGEs can also be prepared in an in vitro environment. F...
Article
Introduction: Development of in vitro models mimicking the physiology and pathophysiology of the brain is essential and remains a major challenge for the discovery of drugs for various neurological diseases. Intensive research efforts over the past two decades led to the development of various 2D/3D in vitro models of the central nervous system an...
Article
In the present investigation, a lactoferrin-conjugated lipid polymer-based nanoparticles (LLPBNPs) encapsulating methotrexate (MTX) as a potential anticancer drugs candidate is constructed via a facile one-step precipitation method. The designed hybrid-nano-bioconjugate exploits both the characteristic features of natural lipids and the biocompatib...
Article
Cryospray is a process of destructing cancerous lesions occurring on the skin. Cryogen is sprayed on the affected area and ablation is achieved through rapid freezing of the cell. Metabolic heat generation, blood perfusion and thermal properties of tissue affect the heat sink in the skin. However, these terms are neglected in in-vitro experiments b...
Article
Bone defects in the craniomaxillofacial (CMF) region occur as a result of trauma, cancer excisions, congenital defects and deformities. CMF bone reconstruction is challenging due to anatomical complexity, functionality and aesthetic conditions. Bone grafting is the current standard approach to reconstruct CMF injuries. However, it possesses certain...
Article
Solid supported catalysts such as amines has high demand for chemical fixation of CO2 into commodity chemicals. Here, we demonstrate an accelerated platform for 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU)-ionic liquids (ILs) catalyzed CO2...
Article
Full-text available
The rationale behind the success of nickel free or with extremely low nickel austenitic high manganese and nitrogen stabilized stainless steels is adverse influences of nickel ion on human body. Replacement of nickel by nitrogen and manganese provides a stable microstructure and facilitates better biocompatibility in respect of the conventional 316...
Article
The chitosan-folate conjugate was synthesized initially and confirmed by FTIR and NMR spectroscopic studies. Following, docetaxel (DXL) loaded non-targeted, single receptor and dual receptor (folate and EGFR) targeted chitosan nanoparticles were prepared and their shape, particle size, zeta-potential, surface morphology and texture were screened by...
Article
Tissue engineering is a promising approach to overcome the severe worldwide shortage of healthy donor corneas. In this work, we have developed a silk-gelatin composite scaffold using electrospinning and permeation techniques to achieve the properties comparable to cornea analog. In particular, we present the fabrication and comparative evaluation o...
Article
In this study, we report the poly-L-lysine (PLL) coated extended large area p-Si/n-ZnO heterojunction based biosensing device to analyze the influence of functional behaviour of primary cortical neuronal cells. Wherein, the proliferation of rat embryonic neurons and neural stem cells is highly influenced by the physicochemical and structural proper...
Article
Novel glutathione (GSH) redox-sensitive thiolated vitaminE-PEG1000-succinate (TPGH-SH) was synthesized by conjugating TPGS with 4-amino thiophenol (4-ATP) and confirmed by FTIR and NMR studies. Following, docetaxel (DTX) loaded, cetuximab (CTB) conjugated redox sensitive TPGS-SH nanoparticles (TPGS-SH NP) were prepared by dialysis method and screen...
Chapter
Full-text available
Ischemic stroke is a prime spawn of death, physical disability and imparts an immense socioeconomic burden for society. Despite, rigorous experimental and clinical research work over the past few decades, still, therapeutic options are scarce for patients with acute ischemic stroke (AIS). It caused an increase in the percentage of ischemic patients...
Article
We demonstrate cell-substrate interaction on aluminium oxide thin-film in metal-insulator-metal structure followed by the change in dielectric characteristics of Al2O3 as a function of progression of cellular growth. The theoretical calculation of the fabricated biosensor reveals that the changes in the intrinsic elemental parameters are mainly att...
Chapter
Coronavirus disease 2019 (COVID-19) now needs no certain introduction. The whole world is working on the various possibilities involving therapeutic agents to combat COVID-19. Based on the mechanism of COVID-19 causing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pathogenesis, numbers of mediators have been proposed to date. However...
Article
In this study, we report the fabrication of poly-L-lysine (PLL) coated large surface TiO2 and SnO2 based biosensing devices to analyze the influence of the functional behaviour of primary cortical neuronal cells. Through frequency-dependent impedance study, we observed an increase in the impedance values initially most likely due to cell adhesion,...
Article
The present study reports for first time the blending of psyllium husk (PH) powder/gelatin (G) in the polymer‐rich composition of polyvinyl alcohol (PVA) to make an electrospinnable solution. The composite was prepared in 3 different ratios viz., 100% (wt/wt) (PVA + PH), 75% + 25% (PVA + 75PH + 25G) (wt/wt) and 50% + 50% (PVA + 50PH + 50G) (wt/wt)...
Article
The primary goal of this study is to highlight the rheological and mechanical properties of a new blend composed of naturally-derived hydrogel materials- psyllium husk (PH) and gelatin (G) for its potential use in three-dimensional (3D) printing technology. The mixtures were prepared at various weight ratios of 100PH, 75PH + 25G and 50PH + 50G. A s...
Chapter
Rapid diagnosis of infectious diseases and up-to-the-minute commencement of relevant treatments are important factors that not only promote positive changes in the clinical scenario but also the health of the mass at large. Surpassing the time-consuming conventional, straightforward in vitro methods for diagnosing infectious diseases, biosensors ha...
Article
Gelatin is a hydrolysed form of collagen which is categorised as type A (acid hydrolysed) or type B (base hydrolysed), based on method of hydrolysis. In this work, we have studied the comparative properties of electrospun nanofibers of gelatin A (GA) and gelatin B (GB) prepared using a ternary solvent (glacial acetic acid/ethyl acetate/water). In a...
Article
Full-text available
Drug-resistant tuberculosis (TB) is one of the most lethal diseases, and it is imperative to exploit an advanced drug formulation for its effective treatment. This work aims to develop a mannose receptor-targeted bioadhesive chitosan nanoparticles for effective drug-resistant tuberculosis treatment. The clofazimine loaded chitosan nanoparticles wer...
Chapter
Advancement in lifestyle and exponential population growth have evoked competitiveness and struggle for survival, resulting in the elevated levels of physiological stress that notably shows correlation with the rising health disparities within the population. Sustained level of stress based on environmental factors, gender inequalities, competitive...
Article
Aim: To design, optimize and evaluate docetaxel-loaded chitosan nanoparticles with (targeted) and without (nontargeted) cetuximab conjugation for the treatment of non-small-cell lung cancer (NSCLC). Materials & methods: Risk-assessment, optimization, in vitro characterizations, stability assessments, release studies, cell-culture studies were perfo...
Article
As the authors were working on similar projects on liposomes at the same time, the 3D figures of Fig. 3 bi and Fig. 3 bii were inadvertently misplaced.
Article
Triple-negative breast (TNBC) cancer that is upregulated with epidermal growth factor receptor (EGFR), and devoid of both the hormonal receptors and epidermal growth factor receptor 2 (HER 2), has led to a concept of treating TNBC with EGFR-targeted therapeutics. The combination of paclitaxel (PTX) and piperine (PIP) may improve the bioavailability...
Article
Full-text available
In this study, we have developed a wireless, portable, standalone, and simple electric cell-substrate impedance sensing (ECIS) system to analyze in-depth functional aspects of cellular functions on the surface of a co-planar metal electrode coated on conventional glass substrate using a low-cost circuitry and correlated it with an equivalent electr...
Article
Amyloids are a group of proteins that are capable of forming aggregated amyloid fibrils, which is responsible for many neurodegenerative diseases including Alzheimer's disease (AD). In our previous study, synthesis and characterization of star‐shaped poly(D,L‐lactide)‐b‐gelatin (ss‐pLG) have been reported. In the present work, we have extended our...
Article
Biocompatible soy protein isolate/silk fibroin (SPI/SF) nanofibrous scaffolds were successfully fabricated through electrospinning a novel protein blend SPI/SF. Prepared nanofibers were treated with ethanol vapor to obtain an improved water-stable structure. Fabricated scaffolds were characterized through scanning electron microscopy (SEM), Fourier...
Article
Adherent mammalian cells are susceptible to the physiological conditions, i.e., physicochemical and topological properties of the extracellular microenvironment surrounding them. In this paper, cell-substrate interaction is taken as the primary step for exhibiting their optimal functions, including cell adhesion, proliferation, migration, and diffe...
Article
The light absorption and emission characteristics of DNA biodots (DNA-BD), along with biocompatibility, give them a high potential for use in various medical applications, particularly in diagnostic purpose. DNA, under high pressure and temperature, condenses to form luminescent biodots. The objective of this research is to develop DNA-biodots (BD)...
Article
Earlier, we had reported the synthesis and characterization of star-shaped poly(D,L-Lactide)-b-gelatin (ss-pLG) to improve cell adhesion and proliferation but the stability of ss-pLG scaffolds remained a persistent issue. Here we show increase in the stability of ss-pLG using 3- glycidoxypropyl trimethoxysilane (GPTMS) as a covalent cross-linker (h...
Article
Objective: Ischemic stroke is a major cause of death and disability worldwide. Nowadays, electrical impedance spectroscopy is an emerging tool to differentiate between normal and stroke conditions. Approach: In this study, the changes in the bio-impedance spectroscopy using two electrodes method with the varying frequencies from 100 - 35k Hz has...
Article
Full-text available
Objectives The primary objective of the study was to optimize micropatterning environments using the microchannel flowed plasma process for controlling the orientation and behaviour of skeletal muscle cells. We have studied the cellular patterning and alignment of skeletal myoblast cells on the various micropattern widths developed on glass substra...
Article
Full-text available
This paper demonstrates the fabrication of a compartmentalized microfluidic device with docking sites to position a single neuron or a cluster of 5–6 neurons along with varying length of microgrooves and the optimization process for culturing primary mammalian neurons at low densities. The principle of centrifugation was employed to situate cells i...
Article
Full-text available
Psyllium husk or isabgol contains xylan backbone linked with arabinose, rhamnose, and galacturonic acid units (arabinoxylans). In this study, we demonstrate the fabrication and characterization of a macroporous three-dimensional (3D) composite scaffold by mixing psyllium husk powder (PH) and gelatin (G) in different ratios, viz.100 PH, 75/25 PH/G,...
Article
Full-text available
Human milk is considered as a complete food since it constitutes all the major essential nutrients, minerals, and vitamins to sustain the life. Human milk is responsible for the evolution of an efficient immune system and organ development as well as acts as a protective barrier against inflammation and infection. In addition, it is known to be a g...
Article
Full-text available
Most important evaluating criteria for in vitro skeletal muscle models include the extent of differentiation and the degree of alignment in the tissue model. Substrate micropatterning is considered as an effective tool as it recreates in vivo like cellular microenvironment and helps in understanding the fundamental concepts and mechanisms underlyin...
Article
Full-text available
In the present study, we propose a platform for topical wound dressing material using a polydimethylsiloxane (PDMS) scaffold in order to enhance the skin healing process. In vitro co-culture assessment of epidermal-origin mouse B16-F10 melanocyte cells and mouse L929 fibroblast cells in three-dimensional polymeric scaffolds has been carried out tow...
Chapter
Drug delivery is defined as administration of drug component inside the body, and the system adopted for the same is known as drug delivery system. Advancements in the drug delivery system are gaining more attention and popularity due to the use of nanoformulations that enables efficient, effective and specific targeting of the drug. Several drug c...
Article
Full-text available
This study demonstrates the comparison in the method of fabrication and thus evaluates the potential of psyllium husk powder and gelatin-based composite microporous scaffolds for tissue engineering applications. The scaffold is being prepared in three different ratios of 50:50, 75:25 and 100 (w/w of psyllium husk powder and gelatin, respectively) b...
Poster
The Performance of Phenol Red (PR) dye functionalized Mesoporous Silica Nanoparticles (MSNs) as Visual pH indicator was evaluated by embedding it on the Polydimethylsiloxane (PDMS) bed and Whatman filter paper. The MSNs were synthesized by acid catalysed of Tetraethyl orthosilicate (TEOS) in presence of cetyltrimethylammonium bromide (CTAB) surfact...
Article
Full-text available
The aim of this study was to examine the cytotoxicity and biocompatibility of functionalized poly(vinyl chloride) (PVC)/layered double hydroxide (LDH) nanocomposites. The biocompatibility of the LDH-based nanocomposites of thiosulphate PVC (TS-PVC), thiourea PVC (TU-PVC) and sulphite PVC (S-PVC) was assessed via haemolysis and thrombogenicity tests...
Article
Optogenetics is a new and emerging field that involves techniques of optics and genetic engineering to influence cellular functionality. In this work, we have successfully incorporated a non-selective cationic channel channelrhodopsin-2 (ChR2) into human hepatocellular carcinoma (HepG2) cells. A plasmid construct AAV-CAG-ChR2-GFP was used for lipos...
Article
Surface and mechanical properties of the biomaterials are determinants of cellular responses. In our previous study, star-shaped poly(d,l-Lactide)-b-gelatin (ss-pLG) was reported for possessing improved cellular adhesion and proliferation. Here, we extended our investigation to establish the cellular compatibility of gelatin-grafted PDLLA with resp...
Article
Full-text available
In vitro tissue model systems have attracted considerable attention in drug discovery owing to their ability to facilitate identification of promising compounds in the near-physiological environment during drug development. Additive manufacturing helps in mimicking complex geometries including the microarchitecture of the body tissues. Exploiting t...
Article
Biodegradable poly(lactic acid) (PLA) is widely used to fabricate 3D scaffolds for tissue regeneration. However, PLA lacks cell adhering functional moieties, which limit its successful application in tissue engineering. Herein, we have tailored the cell adhesive properties of star shaped poly(D,L-Lactide) (ss-PDLLA) by grafting gelatin to their 4 a...
Article
Pulmonary diseases such as chronic obstructive pulmonary disease, asthma and tuberculosis are among the leading causes of death globally, accounting for nearly 4 million of all deaths worldwide every year. Prevention, management and treatment of these deadly diseases require early detection of pathogens and accurate study of pathophysiology of the...
Article
Trans resveratrol (RSV), a natural molecule isolated from red wine, is widely known for several therapeutic potentials. RSV is proved for cardioprotective effects, vasodilation, anti-inflammatory, anticancer effects. Recently, anti cancer potential against glioma cells has also been reported. However, the clinical application of RSV in glioma treat...
Article
The clinical application of trans resveratrol (RSV) in glioma treatment is largely limited because of its rapid metabolism, fast elimination from systemic circulation and low biological half life. Therefore, the objectives of this study were to enhance the circulation time, biological half life and passive brain targeting of RSV using D-α-tocophery...
Article
On page 1478 S. Shoham and co-workers introduce a "brain-on-a-chip" microfluidic platform that hosts brain-like 3D cultures ("optonets") whose activity and response to flowing drugs are recorded optically. Optonets are viable, optically accessible 3D neural networks whose characteristics approximate cortical networks. The results demonstrate the ab...
Article
The study introduces a "brain-on-a-chip" microfluidic platform that hosts brain-like 3D cultures ("optonets") whose activity and responses to flowing drugs are recorded optically. Optonets are viable, optically accessible 3D neural networks whose characteristics approximate cortical networks. The results demonstrate the ability to monitor complex 3...
Article
Full-text available
Poly(vinyl chloride) (PVC) was surface-modified with three different ionomers including thiosulphate, thiourea and sulphate for improving the biocompatibility of the polymer. All ionomers were prepared by the nucleophilic substitution using a phase transfer catalyst. Modified forms of PVC were characterized using ultraviolet-visible (UV-Vis) spectr...
Patent
A neural network is disclosed. The neural network comprises a plurality of optogenetically modified neural cells being three-dimensionally distributed in a hydrogel medium and being disconnected from any solid support having a shear modulus above 1 GPa.
Article
An optical contact pH sensor integrated with capillary microfluidics is proposed in this paper. The complementary metal-oxide-semiconductor sensing array detects the changes of light intensity after absorption by a phenol red-doped sol-gel filter, while the level of absorbance corresponds to a pH value of the analyte. The optical signal is analyzed...
Patent
A neural network is disclosed. The neural network comprises a plurality of optogenetically modified neural cells being three-dimensionally distributed in a hydrogel medium and being disconnected from any solid support having a shear modulus above 1 GPa.
Article
Abstract In the past few years, promising efforts to utilize microfabrication-based technologies have laid the foundation for developing advanced, and importantly more physiologically-realistic, microfluidic methods for risk assessment of engineered nanomaterials (ENMs). In the present review, we discuss the wave of recent developments using microf...
Article
We investigated the role of flow-induced shear stress on the mechanisms regulating surfactant secretion in type II alveolar epithelial cells (ATII) using microfluidic models. Following flow stimulation spanning a range of wall shear stress (WSS) magnitudes, monolayers of ATII (MLE-12 and A549) cells were examined for surfactant secretion by evaluat...
Article
This paper describes the development of a microfluidic device for axon orientation. The device was fabricated with poly (dimethylsiloxane) by soft lithography and replica molding. There were two separated rooms in the device, and a control system and stable concentration gradient system were maintained in each room for at least 24 h. Finally, to de...
Article
Full-text available
Our current understanding of respiratory physiology and pathophysiological mechanisms of lung diseases is often limited by challenges in developing in vitro models faithful to the respiratory environment, both in cellular structure and physiological function. The recent establishment and adaptation of microfluidic-based in vitro devices (μFIVDs) of...
Article
This work describes the development of a multi-inlet microfluidic device for in situ detection of multiple cytotoxicity endpoints. The device consists of an upstream microfluidic multi-inlet module (μMIM) and a downstream microfluidic cell culture channel (μCCC). The integration of a device with syringe pumps via the inlets of MIM mainly enables th...
Article
In this work, the formation of functional synapses between compartmentalized cortical neurons cultured inside three-compartment microfluidic devices in a controlled fashion is described. The proposed device can direct axons in an isolated compartment and, thus, facilitates isolated axons forming functional synapses with dendrites of other neurons i...
Article
Full-text available
We have investigated the cytotoxic potential of tellurium (Te) nanowires in BALB/3T3 fibroblast cells. Te nanowires were synthesized through an aqueous phase surfactant assisted method. Toxicological experiments, such as analysis of morphological changes, MTT assay, DAPI staining, and estimation of intracellular reactive oxygen species, were carrie...
Article
One of the known cytotoxic lignans is (-)-1-O-feruloyl-secoisolariciresinol designated as hanultarin, which was isolated from the seeds of Trichosanthes kirilowii. In this Letter, we described the first synthesis of 1-O-feruloyl-secoisolariciresinol, 1,4-O-diferuloyl-secoisolariceresinol and their analogues. The cytotoxicities of these compounds we...
Article
This work describes a simple method that generates temporal concentration profiles of chemicals inside a microfluidic channel and exposes cultured cells to chemicals in order to mimic the exponential clearance curve of chemicals in the human body. The device fabricated with a poly (dimethylsiloxane) (PDMS) replica mold and a glass substrate was use...
Article
Full-text available
In this study, we demonstrate a new perspective on in vitro assessment method for evaluating quantum dot (QD) toxicity by using microfluidics technology. A new biomimetic approach, based on the flow exposure condition, was applied in order to characterize the cytotoxic potential of QD. In addition, the outcomes obtained from the flow exposure condi...
Article
Cells respond rapidly to toxic substances by altering the morphology, growth, behavior, and biochemical processes controlling basic cell functions.in vitrocytotoxicity analysis, an alternative to animal-based testing, is an important tool to specifically screen the chemicals and form a comprehensive and reliable toxicological data useful for predic...
Article
This paper describes the cytotoxic effects of surface-modified quantum dots (QDs) on neuronlike PC12 cells under localized exposure conditions. PC12 cells were cultured and differentiated on a two-compartment microfluidic neuronal device with the aim of preferentially isolating axons from their cell bodies and thus providing localized exposure of c...
Article
With the widespread use of quantum dots (QDs), the likelihood of exposure to QDs has been assumed to have increased substantially. Recently, QDs have been employed in numerous biological and medical applications. However, there is a lack of toxicological data pertaining to QDs. In this study, we aimed to investigate the cytocompatibility of surface...
Data
Cytotoxicity assay determined by calcein AM and ethidium homodimer in conventional 12-well plates. Representative fluorescent images showing (a) Control cells. (b) Cells treated with 50 μM CdCl2 for 12 h. (c) Cells, pre-exposed to 500 μM NAC for 2 h, were treated with 50 μM cadmium and 500 μM NAC simultaneously for 12 h. Green color indicates live...
Article
Cell death is the ultimate endpoint of the cellular life cycle, and the ability to distinguish different types of cell death (e.g., apoptosis and necrosis) has been a subject of intensive research. This paper demonstrates the suitability and efficiency of a microfluidics-based method for characterization of cell death events. A multicompartmented m...