Sandra Díaz-SánchezUniversity of Castilla-La Mancha
Sandra Díaz-Sánchez
Doctor of Veterinary Medicine
About
59
Publications
32,003
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1,272
Citations
Introduction
Additional affiliations
September 2013 - December 2013
July 2012 - present
February 2012 - May 2012
Research Institute of Hunting Resources
Position
- PhD Student
Description
- Teaching in graduate studies of the University Master of Basic and Applied Research in Game Resources
Education
August 2007 - January 2012
Research Institute of Hunting Resources
Field of study
- Wildlife diseases
September 2000 - November 2005
Publications
Publications (59)
The α-Gal syndrome (AGS) is an IgE-mediated tick borne-allergy that results in delayed anaphylaxis to the consumption of mammalian meat and products containing α-Gal. Considering that α-Gal-containing microbiota modulates natural antibody production to this glycan, this study aimed to evaluate the influence on tick salivary compounds on the gut mic...
This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of field-collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888), and in geo-referenced sequences obtained from GenBank. Since the tick is curren...
Background:
Alpha-Gal syndrome (AGS) is a tick-borne food allergy caused by IgE antibodies against the glycan galactose-alpha-1,3-galactose (α-Gal) present in glycoproteins and glycolipids from mammalian meat. To advance in the diagnosis and treatment of AGS, further research is needed to unravel the molecular and immune mechanisms underlying this...
Tick microbiota can be targeted for the control of tick-borne diseases such as human granulocytic anaplasmosis (HGA) caused by model pathogen, Anaplasma phagocytophilum. Frankenbacteriosis is inspired by Frankenstein and defined here as paratransgenesis of tick symbiotic/commensal bacteria to mimic and compete with tick-borne pathogens. Interaction...
Background
Excessive and irrational use of antibiotics as growth promoters in poultry has been one of key factors contributing to increased emergence of antibiotics resistant bacteria. Several alternatives for antibiotic growth promoters are being sought, and the search for effective probiotics to be used as feed additives is amongst the promising...
Anaplasma phagocytophilum Major surface protein 4 (MSP4) plays a role during infection and multiplication in host neutrophils and tick vector cells. Recently, vaccination trials with the A. phagocytophilum antigen MSP4 in sheep showed only partial protection against pathogen infection. However, in rabbits immunized with MSP4, this recombinant antig...
The range of Red-legged Partridge populations is very wide from natural ones, through those managed for maximum hunting yields, up to completely artificial captive-reared ones. Within this scenario, the Red-legged Partridge has become an interesting model species for studying disease dynamics and host-pathogen evolution. Indeed, exposure to pathoge...
Background
Excessive and irrational use of antibiotics as growth promoters in poultry has been one of key factors contributing to increased emergence of antibiotics resistant bacteria. Drug resistant infections are becoming major concerns in poultry production impacting both human and poultry health. Several alternatives for antibiotic growth promo...
This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of purposely collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888) introduced in the Nearctic-Neotropical range (Mexico to Argentina), and in g...
Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water qualit...
The lack of tools for the precise manipulation of the tick microbiome is currently a major limitation to achieve mechanistic insights into the tick microbiome. Anti-tick microbiota vaccines targeting keystone bacteria of the tick microbiota alter tick feeding, but their impact on the taxonomic and functional profiles of the tick microbiome has not...
Anti-tick microbiota vaccines have been shown to impact tick feeding but its specificity has not been demonstrated. In this study we aimed to investigate the impact of immune targeting of keystone microbiota bacteria on tick performance, and tick microbiota structure and function. Vaccination against Escherichia coli, the selected keystone taxon, i...
Coronavirus-like organisms have been previously identified in Arthropod ectoparasites (such as ticks and unfed cat flea). Yet, the question regarding the possible role of these arthropods as SARS-CoV-2 passive/biological transmission vectors is still poorly explored. In this study, we performed in silico structural and binding energy calculations t...
Exploring tick associations with complex microbial communities and single-microbial partners, especially intracellular symbionts, has become crucial to understand tick biology. Of particular interest are the underlying interactions with biological consequences i.e. tick fitness, vector competence. In this study, we first sequenced the 16S rRNA bact...
Ticks are arthropod ectoparasite vectors of pathogens and the cause of allergic reactions affecting human health worldwide. In humans, tick bites can induce high levels of immunoglobulin E antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate anaphylactic reactio...
The interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design, close to fresh produce, and growers frequently move between the two production environments. The primary objectives of...
Recently, our knowledge of the composition and complexity of tick microbial communities has increased and supports microbial impact on tick biology. Results support a phylogenetic association between ticks and their microbiota across evolution; this is known as phylosymbiosis. Herein, using published datasets, we confirm the existence of phylosymbi...
The poultry red mite, Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of...
Much work has been dedicated to identifying members of the microbial gut community that have potential to augment the growth rate of agricultural animals including chickens. Here, we assessed any correlations between the fecal microbiome, a proxy for the gut microbiome, and feed efficiency or weight gain at the pedigree chicken level, the highest t...
Classification model statistics.
(DOCX)
Chloroplast associated reads differ significantly between high and low graded Line A chickens.
A darker tinted background indicates a significant difference within that genetic line between high and low chickens. Significance is defined as a p-value less than or equal to 0.05 using a Mann-Whitney U-test.
(EPS)
Shannon Diversity does not significantly differ between high and low graded chickens in most cases.
In this figure we present the alpha diversity (calculated as Shannon diversity) for high and low graded Line A (A) and Line B (B) chickens; as well as Line A (C) and Line B (D) chickens graded using the feed conversion metric. Significance is defined...
Supplementary methods used in this work.
(DOCX)
OBJECTIVE We proposed to explore the hypothesis of congruence between host phylogeny and microbial community. Our study model was based on seven species within the family Ixodidae with metagenomic datasets publicly available. HOST-PHYLOGENY was constructed based on cox1 seqs downloaded from the NCBI, aligned with CLUSTALw and tree building in RaxML...
Exploring the microbial diversity of ticks is crucial to understand geographical dispersion and pathogen transmission. Tick microbes participate in many biological processes implicated in the acquisition, maintenance, and transmission of pathogens, and actively promote host phenotypic changes, and adaptation to new environments. The microbial commu...
An innovative metaomics approach integrating metatranscriptomics and metaproteomics was used to characterize bacterial communities in the microbiota of the Lyme borreliosis spirochete vector, Ixodes ricinus (Acari: Ixodidae). Whole internal tissues and salivary glands from unfed larvae and female ticks, respectively were used. Reused I. ricinus RNA...
Here, we report the draft genome sequences of isolates of Anaplasma phagocytophilum , Anaplasma marginale , and Anaplasma ovis . The genomes of A. phagocytophilum (human), A. marginale (cattle), and A. ovis (goat) isolates from the United States were sequenced and characterized. This is the first report of an A. ovis genome sequence.
When prebiotics and other fermentation substrates are delivered to animals as feed supplements, the typical goal is to improve weight gain and feed conversion. In this work, we examined pasture flock chicken cecal contents using next generation sequencing (NGS) to identify and understand the composition of the microbiome when prebiotics and ferment...
The zoonotic pathogen, Campylobacter, is the leading cause of foodborne illness worldwide. It colonizes the intestinal tract of chickens, its primary host. In chickens, this pathogen is considered commensal and does not cause illness or have adverse impacts on production performance. A number of methods aim at controlling Campylobacter in the farm....
Las garrapatas han prosperado en las últimas décadas de forma alarmante gracias al cambio climático, ocasionando importantes pérdidas económicas y transmitiendo peligrosas enfermedades al hombre como la fiebre hemorrágica de Crimea-Congo, enfermedad de Lyme, encefalitis vírica, la anaplasmosis granulocítica humana y un largo etcétera. El cazador se...
Due to the significance of the microbiome on human health, much of the current data available regarding microbiome functionality is centered on human medicine. For agriculturally important taxa, the functionality of gastrointestinal bacteria has been studied with the primary goals of improving animal health and production performance. With respect...
Antibiotics are used in poultry production not only for therapeutic purposes; some producers also administer sub-therapeutic dosages for growth promoting purposes, and residues can be detected in eggs and poultry meat if proper withdrawal protocols are not followed. Furthermore, zoonotic bacteria may acquire resistance to antibiotics as a result of...
The development of antibiotic resistant pathogens has resulted from the use of sub-therapeutic concentrations of antibiotics delivered in poultry feed. Furthermore, there are a number of consumer concerns regarding the use of antibiotics in food animals including residue contamination of poultry products and antibiotic resistant bacterial pathogens...
Previously, we reported the use of quinolones in broiler chickens resulted in residues in retail poultry meat obtained from nine districts in the Santiago Province of the Dominican Republic. Residues in poultry products are a concern due to consumer allergies and the potential to develop
antibiotic-resistant bacteria. Given the use of quinolones in...
Background: Probiotic strains of bacteria can prevent Salmonella from causing disease by preventing the pathogen from colonizing the intestines. Two strains of probiotics, Lactobacillus acidophilius and Pediococcus spp, that were obtained from poultry fecal samples have been shown to be efficacious in poultry. The objective of this study was to det...
Due to the scarcity of water resources in the “Mancha Húmeda” Biosphere Reserve, the use of treated wastewater has been proposed
as a solution for the conservation of natural threatened floodplain wetlands. In addition, wastewater treatment plants of
many villages pour their effluent into nearby natural lakes. We hypothesized that certain avian pat...
Yerba mate (Ilex paraguariensis) is a tea known to have beneficial effects on human health and antimicrobial activity against some foodborne pathogens. Thus, the application of yerba mate as a feed additive for broiler chickens to reduce Salmonella colonization was evaluated. The first in vitro evaluation was conducted by suspending Salmonella Ente...
Subtilase cytotoxin (SubAB) is an AB5 toxin produced by Shiga toxin (Stx)-producing Escherichia coli (STEC) strains usually lacking the eae gene product intimin. Two allelic variants of SubAB encoding genes have been described: subAB1, located on a plasmid, and subAB2, located on a pathogenicity island (PAI) together with tia gene. While subAB1 has...
Colibacillosis in different forms is responsible for significant economic losses in the poultry industry worldwide. Escherichia coli strains frequently implicated in poultry disease are designated as avian pathogenic E. coli (APEC). Natural infections and disease due to APEC have been described in wild birds, but not as yet in red-legged partridges...
The era of molecular biology and automation of the Sanger chain-terminator sequencing method has led to discovery and advances in diagnostics and biotechnology. The Sanger methodology dominated research for over 2 decades, leading to significant accomplishments and technological improvements in DNA sequencing. Next-generation high-throughput sequen...
A total of 142 samples of game meat and ready-to-eat meat products from red deer and wild boar were analysed in order to assess the presence of Shiga toxin-producing Escherichia coli (STEC). Shiga-toxin encoding genes (stx genes) were detected by PCR in 36 (25.4%) of the samples and STEC was isolated from 8 (5.6%) of the same samples. None of the s...
Studies exploring the ecological interactions between avian influenza viruses (AIV), natural hosts and the environment are scarce. Most work has focused on viral survival and transmission under laboratory conditions and through mathematical modelling. However, more integrated studies performed under field conditions are required to validate these r...
Average and range values of water parameters by sampling period and location.
(DOCX)
Red-legged partridges (Alectoris rufa) are a significant part of the culture, diet and income for many people in central and southern Spain. Due to declining populations in the wild, intensive farming is common and 4 million juvenile partridges are released each autumn. Intensive management and high densities result in high prevalence of enteric di...
Shiga toxin-producing Escherichia coli (STEC) O128:H2 is recognised worldwide to be an important non-O157 STEC associated with human illness and in particular with causing haemolytic uraemic syndrome. This serotype is commonly isolated from sheep and is being increasingly isolated from deer. We determined the virulence profile and genetic relations...
La perdiz roja (Alectoris rufa) es un ave autóctona emblemática de la Península Ibérica y constituye la especie de caza menor más importante de las zonas rurales del centro-sur de España (Bernabéu, 2000; MARM, 2008). También es, junto con el conejo de monte (Oryctolagus cuniculus), una pieza clave en los ecosistemas mediterráneos de la que dependen...
Fallow deer (Dama dama) are widely distributed as natural or naturalised populations, as well as in game parks and deer farms. We used 157 fallow deer sampled in populations considered to be Mycobacterium tuberculosis complex (MTC) free and 73 Mycobacterium bovis-infected fallow deer confirmed postmortem by culture to evaluate the diagnostic perfor...
The Mancha Húmeda is a wetland district of international importance for birds, but its status as a Biosphere Reserve is under review, because of nonsustainable development, including sewage inputs to natural wetlands. Sewage treatment plants are usually not capable to cope with extreme pollution or storm events; these pulses stimulate plant and inv...
The Mancha Húmeda is a wetland district of international importance for birds, but its status as a Biosphere Reserve is under review, because of nonsustainable development, including sewage inputs to natural wetlands. Sewage treatment plants are usually not capable to cope with extreme pollution or storm events; these pulses stimulate plant and inv...
For hunting purposes, several millions of red-legged partridges (Alectoris rufa) are released each year in Spain, and these releases have the potential to introduce new parasites and disease into wild
populations. We studied the prevalence of Escherichia coli, Campylobacter sp. and Salmonella sp. in the intestinal flora of red-legged partridges fro...