Sandra Díaz-Sánchez

Sandra Díaz-Sánchez
University of Castilla-La Mancha · Sanidad y Biotecnología (SaBio)

Doctor of Veterinary Medicine

About

52
Publications
26,852
Reads
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875
Citations
Additional affiliations
September 2013 - December 2013
University of Tennessee
Position
  • Research Assistant
July 2012 - present
University of Tennessee
Position
  • PostDoc Position
February 2012 - May 2012
Research Institute of Hunting Resources
Position
  • PhD Student
Description
  • Teaching in graduate studies of the University Master of Basic and Applied Research in Game Resources
Education
August 2007 - January 2012
Research Institute of Hunting Resources
Field of study
  • Wildlife diseases
September 2000 - November 2005
Complutense University of Madrid
Field of study
  • Veterinary Medicine

Publications

Publications (52)
Chapter
The range of Red-legged Partridge populations is very wide from natural ones, through those managed for maximum hunting yields, up to completely artificial captive-reared ones. Within this scenario, the Red-legged Partridge has become an interesting model species for studying disease dynamics and host-pathogen evolution. Indeed, exposure to pathoge...
Preprint
This study addresses the variability of the mitochondrial cytochrome oxidase subunit I (COI) and 16S rDNA (16S), and nuclear internal transcriber spacer ITS2 (ITS2) genes in a set of purposely collected samples of the cattle tick, Rhipicephalus microplus (Canestrini, 1888) introduced in the Nearctic-Neotropical range (Mexico to Argentina), and in g...
Article
Full-text available
Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water qualit...
Article
Full-text available
The lack of tools for the precise manipulation of the tick microbiome is currently a major limitation to achieve mechanistic insights into the tick microbiome. Anti-tick microbiota vaccines targeting keystone bacteria of the tick microbiota alter tick feeding, but their impact on the taxonomic and functional profiles of the tick microbiome has not...
Preprint
Full-text available
Anti-tick microbiota vaccines have been shown to impact tick feeding but its specificity has not been demonstrated. In this study we aimed to investigate the impact of immune targeting of keystone microbiota bacteria on tick performance, and tick microbiota structure and function. Vaccination against Escherichia coli, the selected keystone taxon, i...
Article
Full-text available
Coronavirus-like organisms have been previously identified in Arthropod ectoparasites (such as ticks and unfed cat flea). Yet, the question regarding the possible role of these arthropods as SARS-CoV-2 passive/biological transmission vectors is still poorly explored. In this study, we performed in silico structural and binding energy calculations t...
Article
Full-text available
Exploring tick associations with complex microbial communities and single-microbial partners, especially intracellular symbionts, has become crucial to understand tick biology. Of particular interest are the underlying interactions with biological consequences i.e. tick fitness, vector competence. In this study, we first sequenced the 16S rRNA bact...
Article
Full-text available
Ticks are arthropod ectoparasite vectors of pathogens and the cause of allergic reactions affecting human health worldwide. In humans, tick bites can induce high levels of IgE antibodies against the carbohydrate Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) present in glycoproteins and glycolipids from tick saliva that mediate anaphylactic reactions known as t...
Article
Full-text available
The interaction of typical host adapted enteric bacterial pathogens with fresh produce grown in fields is complex. These interactions can be more pronounced in co-managed or sustainable farms where animal operations are, by design, close to fresh produce, and growers frequently move between the two production environments. The primary objectives of...
Article
Full-text available
Recently, our knowledge of the composition and complexity of tick microbial communities has increased and supports microbial impact on tick biology. Results support a phylogenetic association between ticks and their microbiota across evolution; this is known as phylosymbiosis. Herein, using published datasets, we confirm the existence of phylosymbi...
Article
Full-text available
The poultry red mite, Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of...
Article
Full-text available
Much work has been dedicated to identifying members of the microbial gut community that have potential to augment the growth rate of agricultural animals including chickens. Here, we assessed any correlations between the fecal microbiome, a proxy for the gut microbiome, and feed efficiency or weight gain at the pedigree chicken level, the highest t...
Data
Chloroplast associated reads differ significantly between high and low graded Line A chickens. A darker tinted background indicates a significant difference within that genetic line between high and low chickens. Significance is defined as a p-value less than or equal to 0.05 using a Mann-Whitney U-test. (EPS)
Data
Shannon Diversity does not significantly differ between high and low graded chickens in most cases. In this figure we present the alpha diversity (calculated as Shannon diversity) for high and low graded Line A (A) and Line B (B) chickens; as well as Line A (C) and Line B (D) chickens graded using the feed conversion metric. Significance is defined...
Data
Supplementary methods used in this work. (DOCX)
Poster
OBJECTIVE We proposed to explore the hypothesis of congruence between host phylogeny and microbial community. Our study model was based on seven species within the family Ixodidae with metagenomic datasets publicly available. HOST-PHYLOGENY was constructed based on cox1 seqs downloaded from the NCBI, aligned with CLUSTALw and tree building in RaxML...
Article
Exploring the microbial diversity of ticks is crucial to understand geographical dispersion and pathogen transmission. Tick microbes participate in many biological processes implicated in the acquisition, maintenance, and transmission of pathogens, and actively promote host phenotypic changes, and adaptation to new environments. The microbial commu...
Article
Full-text available
An innovative metaomics approach integrating metatranscriptomics and metaproteomics was used to characterize bacterial communities in the microbiota of the Lyme borreliosis spirochete vector, Ixodes ricinus (Acari: Ixodidae). Whole internal tissues and salivary glands from unfed larvae and female ticks, respectively were used. Reused I. ricinus RNA...
Article
Here, we report the draft genome sequences of isolates of Anaplasma phagocytophilum , Anaplasma marginale , and Anaplasma ovis . The genomes of A. phagocytophilum (human), A. marginale (cattle), and A. ovis (goat) isolates from the United States were sequenced and characterized. This is the first report of an A. ovis genome sequence.
Article
Full-text available
When prebiotics and other fermentation substrates are delivered to animals as feed supplements, the typical goal is to improve weight gain and feed conversion. In this work, we examined pasture flock chicken cecal contents using next generation sequencing (NGS) to identify and understand the composition of the microbiome when prebiotics and ferment...
Chapter
Full-text available
The zoonotic pathogen, Campylobacter, is the leading cause of foodborne illness worldwide. It colonizes the intestinal tract of chickens, its primary host. In chickens, this pathogen is considered commensal and does not cause illness or have adverse impacts on production performance. A number of methods aim at controlling Campylobacter in the farm....
Article
Las garrapatas han prosperado en las últimas décadas de forma alarmante gracias al cambio climático, ocasionando importantes pérdidas económicas y transmitiendo peligrosas enfermedades al hombre como la fiebre hemorrágica de Crimea-Congo, enfermedad de Lyme, encefalitis vírica, la anaplasmosis granulocítica humana y un largo etcétera. El cazador se...
Article
Full-text available
Due to the significance of the microbiome on human health, much of the current data available regarding microbiome functionality is centered on human medicine. For agriculturally important taxa, the functionality of gastrointestinal bacteria has been studied with the primary goals of improving animal health and production performance. With respect...
Article
Full-text available
Antibiotics are used in poultry production not only for therapeutic purposes; some producers also administer sub-therapeutic dosages for growth promoting purposes, and residues can be detected in eggs and poultry meat if proper withdrawal protocols are not followed. Furthermore, zoonotic bacteria may acquire resistance to antibiotics as a result of...
Article
Full-text available
The development of antibiotic resistant pathogens has resulted from the use of sub-therapeutic concentrations of antibiotics delivered in poultry feed. Furthermore, there are a number of consumer concerns regarding the use of antibiotics in food animals including residue contamination of poultry products and antibiotic resistant bacterial pathogens...
Article
Full-text available
Previously, we reported the use of quinolones in broiler chickens resulted in residues in retail poultry meat obtained from nine districts in the Santiago Province of the Dominican Republic. Residues in poultry products are a concern due to consumer allergies and the potential to develop antibiotic-resistant bacteria. Given the use of quinolones in...
Article
Full-text available
Background: Probiotic strains of bacteria can prevent Salmonella from causing disease by preventing the pathogen from colonizing the intestines. Two strains of probiotics, Lactobacillus acidophilius and Pediococcus spp, that were obtained from poultry fecal samples have been shown to be efficacious in poultry. The objective of this study was to det...
Article
Full-text available
Due to the scarcity of water resources in the “Mancha Húmeda” Biosphere Reserve, the use of treated wastewater has been proposed as a solution for the conservation of natural threatened floodplain wetlands. In addition, wastewater treatment plants of many villages pour their effluent into nearby natural lakes. We hypothesized that certain avian pat...
Article
Full-text available
Yerba mate (Ilex paraguariensis) is a tea known to have beneficial effects on human health and antimicrobial activity against some foodborne pathogens. Thus, the application of yerba mate as a feed additive for broiler chickens to reduce Salmonella colonization was evaluated. The first in vitro evaluation was conducted by suspending Salmonella Ente...
Article
Subtilase cytotoxin (SubAB) is an AB5 toxin produced by Shiga toxin (Stx)-producing Escherichia coli (STEC) strains usually lacking the eae gene product intimin. Two allelic variants of SubAB encoding genes have been described: subAB1, located on a plasmid, and subAB2, located on a pathogenicity island (PAI) together with tia gene. While subAB1 has...
Article
Full-text available
Colibacillosis in different forms is responsible for significant economic losses in the poultry industry worldwide. Escherichia coli strains frequently implicated in poultry disease are designated as avian pathogenic E. coli (APEC). Natural infections and disease due to APEC have been described in wild birds, but not as yet in red-legged partridges...
Article
Full-text available
The era of molecular biology and automation of the Sanger chain-terminator sequencing method has led to discovery and advances in diagnostics and biotechnology. The Sanger methodology dominated research for over 2 decades, leading to significant accomplishments and technological improvements in DNA sequencing. Next-generation high-throughput sequen...
Article
A total of 142 samples of game meat and ready-to-eat meat products from red deer and wild boar were analysed in order to assess the presence of Shiga toxin-producing Escherichia coli (STEC). Shiga-toxin encoding genes (stx genes) were detected by PCR in 36 (25.4%) of the samples and STEC was isolated from 8 (5.6%) of the same samples. None of the s...
Article
Full-text available
Studies exploring the ecological interactions between avian influenza viruses (AIV), natural hosts and the environment are scarce. Most work has focused on viral survival and transmission under laboratory conditions and through mathematical modelling. However, more integrated studies performed under field conditions are required to validate these r...
Data
Average and range values of water parameters by sampling period and location. (DOCX)
Article
Full-text available
Red-legged partridges (Alectoris rufa) are a significant part of the culture, diet and income for many people in central and southern Spain. Due to declining populations in the wild, intensive farming is common and 4 million juvenile partridges are released each autumn. Intensive management and high densities result in high prevalence of enteric di...
Article
Shiga toxin-producing Escherichia coli (STEC) O128:H2 is recognised worldwide to be an important non-O157 STEC associated with human illness and in particular with causing haemolytic uraemic syndrome. This serotype is commonly isolated from sheep and is being increasingly isolated from deer. We determined the virulence profile and genetic relations...
Conference Paper
Full-text available
La perdiz roja (Alectoris rufa) es un ave autóctona emblemática de la Península Ibérica y constituye la especie de caza menor más importante de las zonas rurales del centro-sur de España (Bernabéu, 2000; MARM, 2008). También es, junto con el conejo de monte (Oryctolagus cuniculus), una pieza clave en los ecosistemas mediterráneos de la que dependen...
Article
Full-text available
Fallow deer (Dama dama) are widely distributed as natural or naturalised populations, as well as in game parks and deer farms. We used 157 fallow deer sampled in populations considered to be Mycobacterium tuberculosis complex (MTC) free and 73 Mycobacterium bovis-infected fallow deer confirmed postmortem by culture to evaluate the diagnostic perfor...
Conference Paper
Full-text available
The Mancha Húmeda is a wetland district of international importance for birds, but its status as a Biosphere Reserve is under review, because of nonsustainable development, including sewage inputs to natural wetlands. Sewage treatment plants are usually not capable to cope with extreme pollution or storm events; these pulses stimulate plant and inv...
Data
The Mancha Húmeda is a wetland district of international importance for birds, but its status as a Biosphere Reserve is under review, because of nonsustainable development, including sewage inputs to natural wetlands. Sewage treatment plants are usually not capable to cope with extreme pollution or storm events; these pulses stimulate plant and inv...
Article
Full-text available
For hunting purposes, several millions of red-legged partridges (Alectoris rufa) are released each year in Spain, and these releases have the potential to introduce new parasites and disease into wild populations. We studied the prevalence of Escherichia coli, Campylobacter sp. and Salmonella sp. in the intestinal flora of red-legged partridges fro...

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Projects

Projects (2)
Project
This Project builds on our previous experience integrating wild bird ecology into AIV surveillance and epidemiology. Our aim is to study the ecology of peridomestic wild bird species in game and poultry farm surroundings as well as urban park settings. We plan to integrate this information with AIV prevalence on a local (from targeted sampling at our study sites) and global scale (using available data on host capacity for AIV for the species).
Project
Farm-rearing and reléase of red-legged partridge for restocking of natural populations is common practice. We explore the risks of transmission of enterobacteria that carry antimicrobial resitance mechanisms at the interface between farm-reared and wil red-legged partridges and step birds, as well as the risks involved for gamebird consumers.