Sandhya Gangaraju

Sandhya Gangaraju
Arizona State University | ASU · Center of Immunology, vaccines and virotherapy

M.Sc, University of Ottawa, Canada

About

26
Publications
36,187
Reads
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834
Citations
Additional affiliations
October 2016 - November 2016
Arizona State University
Position
  • Principal research specialist
March 2014 - present
Arizona State University
Position
  • Principal research specialist
Description
  • Principal research specialist in biochemistry and cell biology, compliance officer and lab manager.
February 2004 - April 2013
National Research Council Canada
Position
  • Technical Officer

Publications

Publications (26)
Article
Full-text available
Physical forces associated with spaceflight and spaceflight analogue culture regulate a wide range of physiological responses by both bacterial and mammalian cells that can impact infection. However, our mechanistic understanding of how these environments regulate host-pathogen interactions in humans is poorly understood. Using a spaceflight analog...
Article
Full-text available
Tissues and organs provide the structural and biochemical landscapes upon which microbial pathogens and commensals function to regulate health and disease. While flat two-dimensional (2-D) monolayers composed of a single cell type have provided important insight into understanding host-pathogen interactions and infectious disease mechanisms, these...
Article
Full-text available
Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in t...
Article
Full-text available
The inflammasome is a caspase-1-activating complex that is implicated in a growing number of acute and chronic pathologies. Interest has increased in identifying small molecular inhibitors of inflammasome signaling because of its role in clinically relevant diseases. It was recently reported that the protein tyrosine kinase, Syk, regulates pathogen...
Article
Full-text available
P2X7 purinergic receptor engagement with extracellular ATP induces transmembrane potassium and calcium flux resulting in assembly of the NLRP3 inflammasome in LPS-primed macrophages. The role of potassium and calcium in inflammasome regulation is not well understood, largely due to limitations in existing methods for interrogating potassium in real...
Article
Full-text available
Semaphorin 3A (Sema3A) increased significantly in mouse brain following cerebral ischemia. However, the role of Sema3A in stroke brain remains unknown. Our aim was to determine wether Sema3A functions as a vascular permeability factor and contributes to ischemic brain damage. Recombinant Sema3A injected intradermally to mouse skin, or stereotactica...
Article
Although the support for the use of antioxidants, such as coenzyme Q10 (CoQ10), to treat Parkinson's disease (PD) comes from the extensive scientific evidence, the results of conducted thus far clinical trials are inconclusive. It is assumed that the efficacy of CoQ10 is hindered by insolubility, poor bioavailability, and lack of brain penetration....
Article
Full-text available
membrane domain-specific signaling events, maintaining synapses and dendritic spines. The purpose of this study is to examine the neuronal response to membrane raft disruption. Membrane rafts of 8 DIV primary neuronal cultures were isolated based on the resistance to Triton X-100 and ability to float in sucrose gradients. Membrane rafts from primar...
Article
Full-text available
Gangliosides, a member of the glycosphingolipid family, are heterogeneously expressed in biological membranes and are particularly enriched within the central nervous system. Gangliosides consist of mono- or poly-sialylated oligosaccharide chains of variable lengths attached to a ceramide unit and are found to be intimately involved in brain diseas...
Article
Membrane rafts, rich in sphingolipids and cholesterol, are membrane microdomains important in neuronal domain-specific signaling events such as during axonal outgrowth and neuronal death. The present study seeks to determine the spatiotemporal association of several axonal guidance signaling molecules with membrane rafts. These molecules are Neurop...
Article
In recent years, GDNF has emerged as a protective and restorative agent in several models of neurodegeneration; however, the exact molecular mechanisms responsible for these effects are not yet fully understood. Here we examined the effects of astrocytes secreting GDNF on neurons subjected to 6OHDA toxicity using in vitro neuron-astroglia co-cultur...
Article
Every year thousands of people suffer from brain injuries and stroke, and develop motor, sensory, and cognitive problems as a result of neuronal loss in the brain. Unfortunately, the damaged brain has a limited ability to enact repair and current modes of treatment are not sufficient to offset the damage. An extensive list of growth factors, neurot...
Article
Full-text available
Mitochondrial fusion in higher eukaryotes requires at least two essential GTPases, Mitofusin 1 and Mitofusin 2 (Mfn2). We have created an activated mutant of Mfn2, which shows increased rates of nucleotide exchange and decreased rates of hydrolysis relative to wild type Mfn2. Mitochondrial fusion is stimulated dramatically within heterokaryons expr...
Article
Full-text available
Thesis (M.Sc.)--University of Ottawa, 2003. Includes bibliographies.

Questions

Questions (4)
Question
I would like to know if anyone is aware of a method to detect hemolysis caused by EHEC strain (O157:H7) on sheep blood agar plate? I am trying to perform phenotypic charcterization of this strain but didn't see any obvious hemolysis on sheep blood agar plates. I am not sure if I am missing something important in this method. Any help is appreciated! Thanks a lot! Sandhya
Question
I could find the transwell inserts ranging from 0.4 to 12um pore size but nothing less than that! Any suggestions? Thanks !

Projects

Projects (4)
Archived project
Sensitive regulation of ion gradients is necessary to mediate rapid activation of the inflammasome for innate immune response, while also preventing aberrant activation and disease. The role of potassium and calcium gradients in the regulation of inflammasome signaling has been an area of great interest to groups studying all aspects of inflammasome biology and its relevance to disease. Here, we developed and applied tools to interrogate the specific roles of potassium and calcium in real-time in living cells stimulated to undergo inflammasome activation.