Samuel Furse

Samuel Furse
Royal Botanic Gardens, Kew · Jodrell Laboratory

PhD

About

60
Publications
12,818
Reads
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559
Citations
Introduction
My research is focused on understanding how lipid metabolism is controlled and what happens when it goes wrong. I have worked on a number of systems, developed methods for sample reparation/data collection and developed a tool for systemic analysis of lipid metabolism called Lipid Traffic Analysis. I apply this to the metabolism of lipids in mammals and in bees.
Additional affiliations
November 2020 - present
Royal Botanic Gardens, Kew
Position
  • PostDoc Position
November 2020 - present
University of Cambridge
Position
  • Researcher
September 2017 - October 2020
University of Cambridge
Position
  • Research Associate

Publications

Publications (60)
Article
Full-text available
Lipidomics is of increasing interest in studies of biological systems. However, high-throughput data collection and processing remains non-trivial, making assessment of phenotypes difficult. We describe a platform for surveying the lipid fraction for a range of tissues. These techniques are demonstrated on a set of seven different tissues (serum, b...
Article
Full-text available
In this paper we present an investigation of parental-diet-driven metabolic programming in offspring using a novel computational network analysis tool. The impact of high paternal carbohydrate intake on offsprings’ phospholipid and triglyceride metabolism in F1 and F2 generations is described. Detailed lipid profiles were acquired from F1 neonate (...
Preprint
Full-text available
The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. In this study, we tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure would form part the control of the cell cycle. Li...
Article
Full-text available
This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analy...
Article
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The underlying mechanisms driving paternally-programmed metabolic disease in offspring remain poorly defined. We fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. Using artificial insemination, in combination with vasectomised male mating, we g...
Article
Full-text available
Much evidence for diabetes mellitus being associated with dysregulated lipid metabolism has been accrued from studies using blood plasma. However, the systemic dysregulation these results point to is not understood. This study used Lipid Traffic Analysis on data from a mouse model of diabetes to test the hypothesis that the systemic control of lipi...
Article
Full-text available
Genome‐wide association studies in adults have identified variants in hydroxysteroid 17‐beta dehydrogenase 13 (HSD17B13) and mitochondrial amidoxime reducing component 1 (MTARC1) as protective against nonalcoholic fatty liver disease (NAFLD). We aimed to test their association with pediatric NAFLD liver histology and investigate their function usin...
Article
Context Gestational diabetes (GDM) affects 20 million women/year worldwide and is associated with childhood obesity. Infants of affected mothers have increased adiposity from birth, which leads to obesity in later life. However, it remains unknown if the effect of GDM upon neonatal body composition is due to hyperglycemia alone, or is mediated by o...
Article
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Objective: Non-alcoholic fatty liver disease (NAFLD) is an increasingly common condition in children characterized by insulin resistance and altered lipid metabolism. Affected patients are at increased risk of cardiovascular disease (CVD) and children with NAFLD are likely to be at risk of premature cardiac events. Evaluation of the plasma lipid p...
Article
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Background Gestational diabetes is associated with increased risk of type 2 diabetes mellitus and cardiovascular disease for the mother in the decade after delivery. However, the molecular mechanisms that drive these effects are unknown. Recent studies in humans have shown that lipid metabolism is dysregulated before diagnosis of and during gestati...
Article
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Objective Poly-unsaturated fatty acid (PUFA) supplements have been trialled as a treatment for a number of conditions, and produced a variety of results. This variety is ascribed to both the supplements, often comprising mixtures of fatty acids and to different effects in different organs. Here, we tested the hypothesis that supplementation of indi...
Article
Full-text available
Background The paternal diet affects lipid metabolism in offspring for at least two generations through nutritional programming. However, we do not know how this is propagated to the offspring. Objectives We tested the hypothesis that the changes in lipid metabolism that are driven by paternal diet are propagated through spermatozoa and not semina...
Article
Full-text available
Maternal obesity during pregnancy has immediate and long-term detrimental effects on the offspring heart. In this study, we characterized the cardiac and circulatory lipid profiles in late gestation E18.5 fetuses of diet-induced obese pregnant mice and established the changes in lipid abundance and fetal cardiac transcriptomics. We used untargeted...
Article
Full-text available
The aim of the current study was to test the hypothesis that maternal lipid metabolism was modulated during normal pregnancy and that these modulations are altered in gestational diabetes mellitus (GDM). We tested this hypothesis using an established mouse model of diet-induced obesity with pregnancy-associated loss of glucose tolerance and a novel...
Article
Full-text available
Detailed molecular analysis is of increasing importance in research into the regulation of biochemical pathways, organismal growth and disease. Lipidomics in particular is increasingly sought after as it provides insight into molecular species involved in energy storage, signalling and fundamental cellular structures. This has led to the use of a r...
Article
Aims: We assessed metabolomic signatures in maternal and cord blood associated with suboptimal outcomes in the continuous glucose monitoring in women with type 1 diabetes in pregnancy trial (CONCEPTT). Methods: Serum samples from 162 mothers (12, 24 and 34 weeks’ gestation) and 93 cord blood samples were analysed for 1049 metabolites and 1041 lipid...
Article
The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure is important for progress through and perhaps even control of th...
Preprint
Full-text available
Maternal obesity during pregnancy has immediate and long-term detrimental effects on the offspring heart. In this study, we characterized the cardiac and circulatory lipid profiles in fetuses of diet-induced obese pregnant mice and established the changes in lipid abundance and fetal cardiac transcriptomics. We used untargeted and targeted lipidomi...
Preprint
Full-text available
Detailed molecular analysis is of increasing importance in research into the regulation of biochemical pathways, organismal growth and disease. Lipidomics in particular is increasingly sought after as it provides insight into molecular species involved in energy storage, signalling and fundamental cellular structures. This has led to the use of a r...
Article
Full-text available
Background Altered lipid metabolism in early life has been associated with subsequent weight gain and predicting this could aid in obesity prevention and risk management. Here, a lipidomic approach was used to identify circulating markers for future obesity risk in translational murine models and validate in a human infant cohort. Methods Lipidomi...
Preprint
Full-text available
Mortality in children with severe malnutrition is strongly related to signs of metabolic dysfunction, such as hypoglycemia. Lower circulating tryptophan levels in children with severe malnutrition suggest a possible disturbance in the tryptophan-nicotinamide (TRP-NAM) pathway and subsequently NAD+ dependent metabolism regulator sirtuin1 (SIRT1). We...
Article
Full-text available
Dried blood spots (DBS) and dried milk spots (DMS) represent convenient matrices for collecting and storing human samples. However, the use of these sample types for researching lipid metabolism remains relatively poorly explored, and especially unclear is the efficiency of lipid extraction in the context of high throughput, untargeted lipidomics....
Article
Dysregulation of the biosynthesis of cholesterol and other lipids has been implicated in many neurological diseases, including Parkinson's disease. Misfolding of α‐Synuclein (α‐Syn), the main actor in Parkinson's, is associated with changes in a lipid environment. However, the exact molecular mechanisms underlying cholesterol effect on α‐Syn bindin...
Article
Full-text available
The global bivalve shellfish industry makes up 25% of aquaculture, is worth USD $17.2 billion year−1, and relies upon a supply of juvenile bivalves produced by adult broodstock in hatcheries. Today large quantities of live algae are grown to feed broodstock at $220 kg−1, driving highly unsustainable energy and resource use. New advances in algal an...
Article
Full-text available
Extraction of the lipid fraction is a key part of acquiring lipidomics data. High-throughput lipidomics, the extraction of samples in 96w plates that are then run on 96 or 384w plates, has particular requirements that mean special development work is needed to fully optimise an extraction method. Several methods have been published as suitable for...
Article
Full-text available
While the consequences of poor maternal diet on the offspring’s cardio-metabolic health have been studied in detail, the role of the father’s diet on the health of his offspring is poorly understood. We used a known mouse model to establish the impact of an isocaloric paternal low-protein high-carbohydrate diet on the offspring’s lipid metabolism....
Preprint
Full-text available
Dried blood spots (DBS) and dried milk spots (DMS) represent convenient matrices for collecting and storing human samples. However, the use of these sample types for researching lipid metabolism remains relatively poorly explored, and especially the efficiency of lipid extraction is unclear. A visual inspection of punched DBSs after standard extrac...
Preprint
Full-text available
Background & aims: Genome-wide association studies in adults have identified variants in HSD17B13 and MARC1 as protective against NAFLD. It is not known if they are similarly protective in children and, more generally, whether the peri-portal inflammation of pediatric NAFLD and lobular inflammation seen in adults share common genetic influences. Th...
Article
Full-text available
Objectives Malnutrition contributes to 45% of the deaths of children < 5. Mortality in severe malnutrition is often related to metabolic disturbances, including hypoglycemia, indicating hepatic metabolic dysfunction. Children with severe malnutrition have been found to have significantly lower circulating tryptophan (TRP) levels in their blood. Dis...
Preprint
Full-text available
Background: Non-alcoholic fatty liver disease (NAFLD) is a condition characterised by insulin resistance and altered lipid metabolism. Evaluation of the serum lipid profile of children with NAFLD offers the opportunity to understand these perturbations and identify biomarkers to distinguish non-alcoholic steatohepatitis (NASH) and fibrosis from sim...
Article
Gestational diabetes (GDM), a common pregnancy complication associated with obesity and long-term health risks, is usually diagnosed at approximately 28 weeks of gestation. An understanding of lipid metabolism in women at risk of GDM could contribute to earlier diagnosis and treatment. We tested the hypothesis that altered lipid metabolism at the b...
Article
Full-text available
We tested the hypothesis that both postnatal feeding and conditions in utero affect lipid metabolism in infants. Infants who experienced restrictive growth conditions in utero and others exposed to maternal hyperglycaemia were compared to a control group with respect to feeding mode. Dried blood spots were collected from a pilot subset of infant pa...
Article
Full-text available
Introduction This study was motivated by the report that infant development correlates with particular lipids in infant plasma. Objective The hypothesis was that the abundance of these candidate biomarkers is influenced by the dietary intake of the infant. Methods A cohort of 30 exclusively-breastfeeding mother–infant pairs from a small region of...
Article
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There is mounting evidence that the consumption of fermented dairy products such as cheese and yoghurt is associated with a reduced risk of type II diabetes. This effect is greater than in fresh milk and differs between cheese and yoghurt. However, the molecular components responsible for the effect are not known. We tested the hypothesis that the...
Preprint
Full-text available
Dysregulation of the biosynthesis of cholesterol and other lipids has been implicated in various neurological diseases, including Parkinson's disease, where the misfolding of membrane-associated α-Synuclein is a key molecular event. A mounting body of evidence suggests that α-Synuclein aggregation is strongly influenced by the lipid environment. Th...
Article
Full-text available
We tested the hypothesis that the lipid composition of infant formula is consistent between manufacturers, countries and target demographic. We developed techniques to profile the lipid and glyceride fraction of milk and formula in a high throughput fashion. Formula from principal brands in the UK (2017–2019; bovine-, caprine-, soya-based), the Net...
Article
Full-text available
Rationale In this paper, we describe a novel method for preparing milk samples and profiling the triglyceride fraction from them. This method was used to explore how the triglyceride (TG) profile of milk modulates as lactation progresses and how the TG profile differs between breasts. Methods Fresh milk was spotted onto Whatman filter paper and ai...
Article
Full-text available
Breastfeeding is regarded as the ideal way to nourish infants. However, feeding with formula milk is also common in much of the West. Despite this, the function of the molecular components of breast and formula milks are not fully understood, less still the relationship between the composition of the milk and the infant's metabolism and how this in...
Article
Full-text available
Background: Imprinted genes, which are expressed in a parent of origin-specific manner, are thought to mediate the genetic priorities of each parent in pregnancy. Recently we reported that some fetal imprinted gene variants are associated with maternal glucose concentrations and blood pressures in pregnancy. We suggest that the conflict between th...
Article
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This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation of the Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour of model lipid systems that describe these modulations in lipid composition indicate that the average stored curvature elastic stress of the membrane is...
Article
Successful passage through the cell cycle presents a number of structural challenges to the cell. Inceptive studies carried out in the last five years have produced clear evidence of modulations in the lipid profile (lipidome) of eukaryotes as a function of the cell cycle. This mounting body of evidence indicates that lipids play key roles in the s...
Article
Full-text available
Lipids are of increasing importance in understanding biological systems. Lipids carrying an anionic charge are noted in particular for their electrostatic interactions with both proteins and divalent cations. However, the biological, analytical, chemical and biophysical data of such species are rarely considered together, limiting our ability to as...
Article
Exploration of the molecular structure of the bacterial cell envelope informs our understanding of its role in bacterial growth. This is crucial for research into both reducing and promoting bacterial growth as well as fundamental studies of cell cycle control. The spatial arrangement of the lipids in the cell envelope of Gram negative bacteria in...
Article
In this paper, we report the inositide-driven formation of an inverse bicontinuous cubic phase with space group Ia3d (QIIG, gyroid phase). The system under study consisted of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine at a molar ratio of 1:49, with a physiological concentration of magnesium ions at pH 7·4. Th...
Article
Neurodegenerative Protein Misfolding Diseases (PMDs), such as Alzheimer's (AD), Parkinson's (PD) and prion diseases, are generally difficult to diagnose before irreversible damage to the central nervous system damage has occurred. Detection of the misfolded proteins that ultimately lead to these conditions offers a means for providing early detecti...
Article
Full-text available
This paper describes a study in which the emulsifying properties of cocoa material with and without its lipid fraction were explored. This study was motivated by the commercial interest in naturally-occurring particulate emulsifiers as opposed to the chemically modified emulsifying particles presently available for commercial use. The hypothesis wa...
Article
This paper describes a study of the phospholipid profile of Escherichia coli MG1655 cultures at the B and D periods of the cell cycle. The results indicate that the phosphatidyl glycerol fraction grows relatively rapidly and that the size of the cardiolipin (CL) fraction does not grow at all during cell elongation. This is consistent with observati...
Article
Since its discovery in the 19th century, phosphatidylcholine (PC) has been regarded primarily as a structural lipid. However, more recent evidence, much of it in the last five years, strongly suggests that PC has other roles. Here, we explore some of that new evidence and consider the possibility that the ultimate role of phosphatidylcholine, and p...
Article
Isolation of the lipid fraction from biological samples has been a crucial part of countless studies over the last century. This considerable research interest has led to the development of a number of methods for isolating a range of molecular species that fall under the umbrella term ‘lipid’. Such methods vary in popularity, complexity, specifici...
Article
Full-text available
In this paper evidence is presented that the fatty acid component of an inositide substrate affects the kinetic parameters of the lipid phosphatase Salmonella Outer Protein B (SopB). A succinct route was used to prepare the naturally occurring enantiomer of phosphatidylinositol 4-phosphate (PI-4-P) with saturated, as well as singly, triply and quad...
Article
After the initial observation that lipids form a considerable part of biological membranes, the details of the physical role of lipids in biological systems have emerged gradually. There have been few ‘Eureka’ moments in which a class or individual lipid has appeared as a game-changing physical player. However, evidence collected in the last five y...
Article
A perennial problem in ‘omics' studies, is ensuring that the molecular profile produced is a true representation of that in the system from which it comes. ... Lipidomics is a relatively new discipline in the ‘omics' field (van Meer et al. 2007, Wenk 2010), that now boasts a number of comprehensive mass spectral studies (Ejsing et al. 2009, Dennis...
Article
Full-text available
In this paper we report the molecular profiling, lipidome and proteome, of the plant organelle known as an oil body (OB). The OB is remarkable in that it is able to perform its biological role (storage of triglycerides) whilst resisting the physical stresses caused by changes during desiccation (dehydration) and germination (rehydration). The molec...
Article
In this paper, we demonstrate a novel and environmentally-conscious approach to the isolation of oil bodies (OBs) from the bran arising from the milling of Oryza sativa (Basmati rice). We have used several physical techniques to determine the effect of the steps of the process on the OBs, and describe an isolation process that is scalable to an ind...
Article
Full-text available
We describe a procedure for isolating and purifying oil bodies (OBs) from Oryza sativa bran, and present evidence that strongly suggests a physical associ-ation between the OB organelles and several antioxidant phytochemicals (c-oryzanol and several tocochromanols). This suggestion arises from work showing that despite comprehensive washing steps (...
Article
In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a function of pressure and temperature has been studied using small-angle X-ray scattering and in the prese...

Questions

Questions (3)
Question
I would like to use PBS with Calcium and Magnesium in order to favour keeping my cells adhered.  However, the HBSS and PBS with CaCl2 and MgCl2 that I have tried all give me cloudy solutions which makes a mess of FACS.
I think the cloudiness is due to the common ion effect, i.e. producing Ca3(PO4)2 and possibly Mg3(PO4)2, which have relatively low solubilities, so perhaps this is just what happens with this type of buffer?
Question
I am looking to stain the PMs of live HeLa cells.  I've tried Texas red attached to PE, but this doesn't appear to have been successful.
Question
I am using HeLa S3 cells and growing adherent cultures on a large scale.
I understand that one also needs an antibody for BrdU, are there any recommendations on which one I might use?  I would also like to know how well the stocks of BrdU and its antibodies keep.
Are the commercially available kits good?

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