Sammy Boussiba

Sammy Boussiba
Ben-Gurion University of the Negev | bgu · Micro algal Biotechnology

phD

About

187
Publications
57,787
Reads
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8,937
Citations
Additional affiliations
April 2015 - present
Ben-Gurion University of the Negev
Position
  • Managing Director
Description
  • Head of the Microalgal Biotechnology laboratory
September 1994 - August 2015
Ben-Gurion University of the Negev
Position
  • Principal Investigator

Publications

Publications (187)
Article
Full-text available
Glutamine synthetase (GlnS) is a key enzyme in nitrogen metabolism. We investigated the effect of the GlnS inhibitor glufosinate on the infection of H. lacustris by the blastocladialean fungus P. sedebokerense, assuming that interfering with the host nitrogen metabolism will affect the success of the parasite. Complete inhibition of infection, whic...
Article
Microalgae were employed to remove nutrients from a pre-treated wastewater (so-called anaerobic membrane bioreactor effluent, AnMBR effluent) over a 24-day semi-continuous cultivation with varied N:P ratios. NH4⁺-N reduction percentage ranged from 68 to 94% with average removal rates varying from 8 to 26 mgN·L⁻¹d⁻¹ at different stages. Total phosph...
Article
Full-text available
CRISPR/Cas9-mediated genome editing has been demonstrated in the model diatom P. tricornutum, yet the currently available genetic tools do not combine the various advantageous features into a single, easy-to-assemble, modular construct that would allow the multiplexed targeting and creation of marker-free genome-edited lines. In this report, we des...
Technical Report
Full-text available
Algae have become a multi-billion sector in terms of biotechnology development that is expected to grow rapidly, providing valuable goods and services in multiple applications. In spite of centuries of scientific and commercial interest, the term algae has not taxonomic meaning. In the light of rapidly growing business associated with algae, a clea...
Article
A near-zero waste treatment system for food processing wastewater was developed and studied. The wastewater was treated using an anaerobic membrane bioreactor (AnMBR), polished using an outdoor photobioreactor for microalgae cultivation (three species were studied), and excess sludge was treated using hydrothermal carbonization. The study was condu...
Article
Full-text available
Several green algae can divide by multiple fission and spontaneously synchronize their cell cycle with the available light regime. The yields that can be obtained from a microalgal culture are directly affected by cell cycle events. Chromochloris zofingiensis is considered as one of the most promising microalgae for biotechnological applications du...
Article
Full-text available
The blastocladialean fungus Paraphysoderma sedebokerense parasitizes three microalgae species of economic interest: Haematococcus pluvialis, Chromochloris zofingiensis and Scenedesmus dimorphus. For the first time, we characterized the developmental stages of isolated fungal propagules in H. pluvialis co-culture, finding a generation time of 16 h....
Article
Full-text available
The green microalga Lobosphaera incisa accumulates triacylglycerols (TAGs) with exceptionally high levels of long-chain polyunsaturated fatty acid (LC-PUFA) arachidonic acid (ARA) under nitrogen (N) deprivation. Phosphorous (P) deprivation induces milder changes in fatty acid composition, cell ultrastructure, and growth performance. We hypothesized...
Article
Full-text available
Microalgae have been considered as a renewable source of nutritional, cosmetic and pharmaceutical compounds. The ability to produce health-beneficial long-chain polyunsaturated fatty acids (LC-PUFA) is of high interest. LC-PUFA and their metabolic lipid mediators, modulate key inflammatory pathways in numerous models. In particular, the metabolism...
Article
Lipid droplets (LDs) are an organelle conserved amongst all eukaryotes, consisting of a neutral lipid core surrounded by a polar lipid monolayer. Many species of microalgae accumulate LDs in response to stress conditions, such as nitrogen starvation. Here we report the isolation and proteomic profiling of LD proteins from the model oleaginous penna...
Article
Full-text available
The dynamic nutrient availability and photon flux density of diatom habitats necessitate buffering capabilities in order to maintain metabolic homeostasis. This is accomplished by the biosynthesis and turnover of storage lipids, which are sequestered in lipid droplets (LDs). LDs are an organelle conserved among eukaryotes, composed of a neutral lip...
Poster
Full-text available
Efficient and economical selection markers are essential tools for genetic engineering and biotechnology. Till date, selection markers for Phaeodactylum tricornutum are dominated by antibiotic resistance genes of bacterial origin. Mutations in phytoene desaturase (PDS1) cause resistance to the herbicide norflurazon. Two novel PDS1 mutants, highly r...
Article
Full-text available
Atherosclerosis and its complications are responsible for one in three global deaths. Nutraceuticals show promise in the prevention and treatment of atherosclerosis but require an indepth understanding of the mechanisms underlying their actions. A previous study showed that the omega-6 fatty acid, dihomo-γ-linolenic acid (DGLA), attenuated atherosc...
Article
The microalga Haematococcus lacustris (formerly H. pluvialis) is the richest source of the valueable pigment astax-anthin, accumulated in red aplanospores (haematocysts). In this work, we report on the photoprotective mechanisms in H. lacustris, conveying this microalga its ability to cope with a wide range of adverse conditions, with special empha...
Article
Haematococcus pluvialis is an industrial green microalga, which presents the best photosynthetic source of the keto-carotenoid astaxanthin due to its high content, reaching over 4% of dry weight. Astaxanthin production by Haematococcus is currently achieved by a two-stage photoautotrophic process, which involves the transition of green biomass to n...
Article
Full-text available
Paraphysoderma sedebokerense (P. sedebokerense) (Blastocladiomycota) is a facultative pathogenic chytrid that causes irreversible damage to some green microalgae. Specific attacks leading to culture collapse under different conditions have only been described in the lucrative microalga Haematococcus pluvialis (H. pluvialis), while generating biomas...
Data
Leaching of pigments from Haematococcus pluvialis cells, before and after autoclaving. Both live (A,B) and autoclave-inactivated cells (C,D) were extracted twice with acetone; pigments were fully recovered only from autoclaved red cells, and the pellet became colorless. Green (A,C) and red (B,D) H. pluvialis cells.
Article
The green oleaginous microalga Lobosphaera incisa accumulates storage lipids triacylglycerols (TAG) enriched in the long-chain polyunsaturated fatty acid arachidonic acid under nitrogen (N) deprivation. In contrast, under phosphorous (P) deprivation, the production of the monounsaturated oleic acid prevails. We compared physiological responses, ult...
Article
Lobosphaera incisa is a green microalga that accumulates high levels of the valuable omega-6 long-chain polyunsaturated fatty acids (LC-PUFA) arachidonic acid (ARA, 20:4n-6) in triacylglycerols (TAG) under nitrogen (N) starvation. LC-PUFA accumulation is a rare trait in photosynthetic microalgae with insufficiently understood physiological signific...
Article
Full-text available
Main conclusion Hypercarotenogenesis in green algae evolved by mutation of PSY that increased its transcription at high light, disintegration of the eyespot in Dunaliella and acquisition of the capacity to export carotenoids from chloroplasts in Haematococcus. Carotenoids (Car) are lipid-soluble pigments synthesized in plants, algae, bacteria and f...
Article
Full-text available
Recent developments in the genetic engineering of biotechnologically important microalgae—among them the diatom Phaeodactylum tricornutum—promise rapid progress in strain improvements for producing various valuable commodities. Nitrogen (N) depletion and starvation are commonly employed strategies to induce or enhance the formation of secondary car...
Article
Full-text available
We report the identification and characterization of an acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1)-encoding gene from the green oleaginous microalga Lobosphaera incisa (SAG 2468), a prolific photosynthetic producer of the n-6 very long chain polyunsaturated fatty acid (VLC-PUFA), arachidonic acid. The gene expression pattern of LiDGAT1 in L....
Article
Studies revealed and keep revealing a staggering diversity of life-cycle patterns and genome-sizes in microalgae. Even within a single order like the Volvocales with Volvox carteri, Chlamydomonas reinhardtii, and Dunaliella salina, differences are significant. Additional knowledge gaps in microalgal physiology and genetics hinder our understanding...
Article
Full-text available
Thraustochytrids, a heterotrophic fungus-like clade of Stramenopiles, are becoming an increasingly important source of polyunsaturated fatty acids (PUFAs) for biotechnological industries. PUFA rich oils from these organisms are subsequently referred to in some literature and marketing sources as being derived from “algae”, in spite of their non-pho...
Article
The oleaginous microalga Lobosphaeraincisa(Trebouxiophyceae, Chlorophyta)contains arachidonic acid (ARA, 20:4 n-6) in all membrane glycerolipids and in the storage lipidtriacylglycerol.The optimal growth temperature of the wild-type (WT) strain is 25°C; chilling temperatures (≤15oC) slow its growth. This effect is more pronounced in the delta-5-des...
Article
Microalgae accumulate the storage lipids triacylglycerols (TAG) in lipid droplets (LDs) in response to environmental cues. Mechanistic insights into LD biogenesis in microalgal cells hold great promise for the manipulation of TAG production. In this report, we demonstrate the heterologous expression of a major LD protein of the green microalga Haem...
Poster
Background: The oleaginous green alga Lobosphaera incisa accumulates high amounts of arachidonic acid (ARA) rich triacylglycerols (TAG) in cytoplasmic lipid droplets (LDs), predominantly under nitrogen starvation conditions. Intensive LD formation is accompanied by dismantling of chloroplast and endomembrane systems and recycling of cellular compon...
Article
Haematococcus pluvialis (Chlorophyta) is a widely used microalga of great economic potential, yet its molecular genetics and evolution are largely unknown. We present new detailed molecular and phylogenetic analysis of two glutamine synthetase (GS) enzymes and genes (gln) under the Astaxanthin-inducing conditions of light- and nitrogen-stress. Stru...
Article
Full-text available
The engagement of different photoprotective mechanisms in the cells of the carotenogenic astaxanthin-accumulating chlorophyte Haematococcus pluvialis (i) under favorable conditions, (ii) in the course of stress-induced haematocyst formation and (iii) during recovery from the stress was studied. To this end, we followed the changes in primary photoc...
Article
Microalgae present a huge and still insufficiently tapped resource of very long-chain omega-3 and omega-6 polyunsaturated fatty acids (VLC-PUFA) for human nutrition and medicinal applications. This chapter describes the diversity of unicellular eukaryotic microalgae in respect to VLC-PUFA biosynthesis. Then, we outline the major biosynthetic pathwa...
Poster
Full-text available
BIOFAT: 1 year of cultivation at a 0.5 ha scale.
Article
Full-text available
Lobosphaera incisa, formerly known as Myrmecia incisa and then Parietochloris incisa, is an oleaginous unicellular green alga belonging to the class Trebouxiophyceae (Chlorophyta). It is the richest known plant source of arachidonic acid, an ω-6 poly-unsaturated fatty acid valued by the pharmaceutical and baby-food industries. It is therefore an or...
Article
Full-text available
The oleaginous green microalga Lobosphaera (formerly Parietochloris) incisa accumulates high amounts of arachidonic-acid-rich triacylglycerols (TAG), in particular under conditions of nitrogen starvation. This photosynthetic organism is of great interest for studying the mechanisms responsible for storage lipid biosynthesis and the deposition of lo...
Article
Full-text available
Nannochloropsis oceanica is an important source for omega-3 long-chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5 n − 3), and a potent candidate for biofuel production, due to its outstanding capability for rapid induction of triacylglycerol (TAG) overproduction. In contrast to membrane lipids, TAG of N. oceanica...
Article
The Δ5 desaturase mutant (P127) of the microalga Lobosphaera incisa is a promising organism for large-scale production of the valuable LC-PUFA dihomo-gamma-linolenic acid (DGLA, 20:3 n-6). We examined the potential of P127 for DGLA production under nitrogen (N) starvation conditions, triggering the deposition of DGLA in triacylglycerols, and develo...
Article
Photosynthetic microalgae are currently the focus of basic and applied research due to an ever-growing interest in renewable energy resources. This review discusses the role of carbon-unit supply for the production of acetyl-CoA, a direct precursor of fatty acid biosynthesis and the primary building block of the growing acyl chains for the purpose...
Article
Full-text available
Abstract We hereby report the complete chloroplast genome sequence of the green unicellular alga Lobosphaera (Parietochloris) incisa (strain SAG 2468). The genome consists of a circular chromosome of 156,028 bp, which is 72% A-T rich and does not contain a large rRNA-encoding inverted repeat. It is predicted to encode a total of 111 genes including...
Patent
Full-text available
The present invention is primarily directed to a mutant strain of Parietochloris incisa, characterized in comprising a substantially reduced intracellular concentration of arachidonic acid (AA) together with a substantially increased intracellular concentration of dihomo-gamma-linolenic acid 20:3ω6 (DGLA).
Article
Full-text available
The chloroplast pyruvate dehydrogenase complex (cpPDC) catalyses the oxidative decarboxylation of pyruvate forming acetyl-CoA, an immediate primer for the initial reactions of de novo fatty acid (FA) synthesis. Little is known about the source of acetyl-CoA in the chloroplasts of photosynthetic microalgae, which are capable of producing high amount...
Article
Full-text available
The green alga Hematococcus pluvialis accumulates large amounts of the antioxidant astaxanthin under inductive stress conditions, such as nitrogen starvation. The response to nitrogen starvation and high light leads to the accumulation of carbohydrates and fatty acids as well as increased activity of the tricarboxylic acid cycle. Although the behav...
Article
Full-text available
Microalgae are considered a promising source for various high value products, such as carotenoids, ω-3 and ω-6 polyunsaturated fatty acids (PUFA). The unicellular green alga Lobosphaera (Parietochloris) incisa is an outstanding candidate for the efficient phototrophic production of arachidonic acid (AA), an essential ω-6 PUFA for infant brain devel...
Article
Full-text available
Microalgae are considered a promising source for various high-value products, including carotenoids and omega-3 and omega-6 polyunsaturated fatty acids (PUFAs). Excluding production by heterotrophic fermentation, only two microalgal high-value products are successfully marketed at a relevant scale: β-carotene from Dunaliella salina, and astaxanthin...
Article
Full-text available
Flexible responses of lipid metabolism to changes in cultivation conditions characteristic of oleaginous microalgae of the genus Nannochloropsis render them a promising source of triacylglycerols for biodiesel (under nutrient-deprivation and high-light stress) and eicosapentaenoic acid (EPA; C20:5, n-3) (under nutrient sufficient conditions). We in...
Article
Full-text available
Using new polymerase chain reaction (PCR) primers, a once known to be under-transcribed microcystin synthetase A (mcyA) gene from the only known toxigenic cyanobacterium Microcystis aeruginosa dominating the Hartbeespoort Dam was consistently amplified from genomic DNA extracted from a set of algal and cell free water samples collected across this...
Article
Full-text available
We investigated the effects of osmotic downshift induced by the transfer of Nannochloropsis oceanica CCALA 804 from artificial seawater medium (27 g L(-1) NaCl) to the same medium without NaCl or freshwater modified BG-11 medium (mBG-11) as a function of photosynthetically active radiation (170, 350, or 700 μmol photon m(-2) s(-1)). Alterations in...