Sameer H Qari

Umm Al-Qura University · Department of Biology

CRISPR/Cas system has emerged as a powerful tool for precise genome editing with numerous applications such as knockout, knock-in, base editing, prime editing and rewriting the genetic code. Cas9 and Cas12 both belong to Class 2 CRISPR/Cas system and among the most widely used Cas nucleases for manipulation of DNA. Although both Cas9 could be used...

Cold stress is one of the most limiting factors for plant growth and development. Cold stress adversely affects plant physiology, molecular and biochemical processes by determining oxidative stress, poor nutrient and water uptake, disorganization of cellular membranes and reduced photosynthetic efficiency. Therefore, to recover impaired plant funct...

The novel coronavirus (2019-nCoV) triggered a worldwide rise in the prevalence of the coronavirus outbreak (COVID-19) and surfaced as a universal wellbeing matter. Analogous with SARS-CoV and MERS-CoV, the main 3-chymotrypsin-alike cysteine protease (3CL Pro) virus enzyme that manages the replications of 2019-nCoV and regulates its existence span,...

Citation: Alnusairi, G.S.H.; Mazrou, Y.S.A.; Qari, S.H.; Elkelish, A.A.; Soliman, M.H.; Eweis, M.; Abdelaal, K.; El-Samad, G.A.; Ibrahim, M.F.M.; ElNahhas, N. Correction: Alnusairi et al. Exogenous Nitric Oxide Reinforces Photosynthetic Efficiency, Osmolyte, Mineral Uptake, Antioxidant, Expression of

Wheat is predominantly used as a staple food all over the world. Stem rust resistance by Sr22 gene is researched and confirmed before but in the current study, its molecular function is predicted by using different in-silico tools such as ExPASy-Translate, ExPASy-PortParam, and InterProScan. We investigated the in-silico functional compositions of...

Growth of crops in the semi-arid and arid regions of the world is significantly affected by salinity stress. The present study investigated maize, which is a globally cultivated crop and known for its salt sensitivity. Widespread cultivation and notable characteristics of maize make it an exemplary model for investigating stress response. We analyz...

The Urticaceae family, encompassing 53 genera and 2625 species, exhibits a subcosmopolitan distribution. Forsskaolea L. is a small genus of seven species. It is represented in Saudi Arabia by two species F. tenacissima L. and F. viridis Ehrenb. The current research elucidates the validity of DNA barcoding in species authintican and taxonomic identi...

Background Crop diversification is considered as an imperative approach for synchronizing the plant nutrient demands and soil nutrient availability. Taking two or more crops from the same field in one year is considered as multiple cropping. It improves the diversity and abundance of soil microbes, thereby improving the growth and yield of crops. T...

The genus Hertia, which belongs to the Asteraceae family, is a flowering genus with 12 species found in Africa, North and South. Among the species present in Algeria, Hertia cheirifolia L. is distributed in the eastern regions of Algeria. The aim of this study is to evaluate its phytochemical composition with following pharmacological assessments:...

In light of the multitude of olive trees cultivated and the lack of the genetic diversity of available genotypes to select varieties and lines that are characterized by high diversity and better performance under the corresponding conditions, A comparison analysis of the genotyping and morphological characteristics of eight olive cultivars growing...

Soil salinization has increased over recent years and is negatively affecting crop productivity. Nutrient application is an effective strategy to improve abiotic stress tolerance in crops. The application of coated fertilizers has emerged as an excellent approach to mitigate the adverse impacts of soil salinity. Therefore, the present study was con...

Sugarcane (Saccharum officinarum) has gained more attention worldwide in recent decades because of its importance as a bioenergy resource and in producing table sugar. However, the production capabilities of conventional varieties are being challenged by the changing climates, which struggle to meet the escalating demands of the growing global popu...

Lipoic acid (LA) is a naturally synthesized antioxidant in plants, and its protective role against abiotic stresses is well known. However, its protective role on tomato growth and yield under salt stress is not clear. In this study, we explored the impact of different doses of LA (0, 2, 4, and 6 mM) on two tomato cultivars (Nadir and Naqeeb) under...

Salicylic acid (SA) is a plant growth regulator known to take part in defense responses against different types of stresses, including salt stress. In this study, the role of foliar applied SA in improving the growth of berseem variety ‘Anmol’ under salt stress was examined. Plants were sown in plastic pots in the sand. Plants were treated with dif...

The increased concentration of lead (Pb) in soils is a serious threat to human beings and plants all over the world. Salinity stress is also a major issue across the globe, which limits crop productivity. The use of allelochemicals has become an effective strategy to mitigate the toxic effects of abiotic stresses. Sorghum is an important crop grown...

Background Pigeon pea is affected by the cyst nematode Heterodera cajani limiting the production. H. cajani can be managed by biological agents such as Trichoderma harzianum . Thus, the determination of this experiment was to estimate native strains of T. harzianum isolated from pigeon pea fields in India on population of cyst, eggs and juveniles o...

Introduction For the first time, differences in ethnobotanical knowledge of medicinal plants between men and women, as well as tribal and urban populations in the Makkah district, are investigated. The current research aims to provide responses to the following questions: (1) According to tribal and urban cultures, which medicinal plants are used b...

The present work aimed to evaluate the potential carcinogenic, cytotoxic, and genotoxic effects of methanolic extract leaves of Frosskaolea tenacissima L. found in Makkah region, Saudi Arabia. The Mitotic Index (MI) was determined for cytotoxicity studies, and micronuclei (MN), chromosomal aberrations (CA) were counted, and a molecular marker (ISSR...

Salinity stress (SS) is a serious abiotic stress and a major constraint to agricultural productivity across the globe. High SS negatively affects plant growth and yield by altering soil physio-chemical properties and plant physiological, biochemical, and molecular processes. The application of micronutrients is considered an important practice to m...

The DUF668 gene performs a critical role in mitigating the impact of abiotic stress factors. In this study, we identified 30 DUF668 genes in a soybean genome, distributed across fifteen chromosomes. The phylogenetic analysis classified the DUF668 genes into three groups (group I, group II, and group III). Interestingly, gene structure analysis illu...

Abutilon hirtum (Lam.) Sweet belongs to the Malvaceae family and is a perennial shrub commonly known as Indian mallow, which is distributed in tropical regions and many different areas in Saudi Arabia. A. hirtum is an economically and a medicinally useful plant in different zones of the world for healing various diseases. The current research explo...

Lipoic acid (LA) is a naturally synthesized antioxidant in plants, and its protective role against abiotic stresses is well known. However, its protective role on tomato growth and yield under salt stress is not clear. In this study, we explored the impact of different doses of LA (0, 2, 4, and 6 mM) on two tomato cultivars (Nadir and Naqeeb) under...

The soybean is a source of several dietary components, including milk, protein, and oil. Cold stress has significantly curtailed soybean growth and yield in large areas and caused a high risk to global food security. The main objective of soybean breeders is to improve soybean resistance to cold stress. Conventional breeding approaches have made si...

Zinc (Zn) is an essential needed for the growth and development of plants, however, Zn is continuously increasing in our soils which is decreasing crop production. Further, the crops grown on Zn-deficient soils also contains a low amount of Zn which is also a major reason for Zn deficiency in humans. So, it is mandatory to supply the Zn to fulfil t...

Soil salinity is abiotic stress of growing concern, whose effects can be potentially mitigated by the use of suitable fertilisers. Based on this, an experiment was conducted to determine the role of vegetable oil–coated urea on the performance of wheat ( Triticum aestivum ) under salinity. Neem oil–coated urea (NOCU), castor oil–coated urea (COCU),...

As a most significant cereal crop, maize provides vital nutritional components to humans and livestock. Drought stress curtails maize growth and yield by impairing several morphological, physiological, and biochemical functions. The rising threats of drought stress significantly affect global food security and increase the ratio of hunger and starv...

Salinity stress (SS) is major abiotic stress that is seriously limiting crop production across the globe. The application of organic amendments (OA) mitigate the effects of salinity and improves soil health and crop production on a sustainable basis. However, limited studies are conducted to determine the impact of farmyard manure (FYM) and press m...

The two-component system (TCS) genes are involved in a wide range of physiological processes in prokaryotes and eukaryotes. In plants, the TCS elements help in a variety of functions, including cell proliferation, response to abiotic and biotic stresses, leaf senescence, nutritional signaling, and division of chloroplasts. Three different kinds of...

Tomato is one of the most significant vegetable crops, which provides several important dietary components. Pakistan has a significant low tomato yield compared to other countries because of low genetic diversity and the absence of improved cultivars. The present study aimed to investigate the genetic variability, heritability, and genetic advance...

The proteins containing the domain of unknown functions (DUFs) have significant roles in stress response and the growth of plants. A comprehensive genome-wide analysis and expression profiling of DUF599 was conducted to identify their roles in potato growth and response to stressed conditions. A total of nine DUF599 genes were identified, located o...

Drought stress is a significant abiotic factor influencing maize growth and development. Understanding the molecular mechanism of drought tolerance is critical to develop the drought tolerant genotype. The identification of the stress responsive gene is the first step to developing a drought tolerant genotype. The aim of the current research was to...

Globally, stored grain is vulnerable to pest infestation, resulting in significant economic losses for some crops. Wheat is one of the most important crops in the world. Many sucking, piercing insects infect wheat in the form of grains or flour and may produce toxic residues that are harmful to human health. The current study aimed to estimate the...

UV mutagenesis has long been known to improve bacterial strains in their physiological capacity. In the current study, we used the UV mutagenesis approach to increase the PGPR characteristics of a pre-characterized metal-tolerant PGPR strain Bacillus sp. strain SR-2-1/1 (KY315919), with the objective of increasing the physiological outcome of its P...

Plant abiotic stresses endanger crop production and food security to a growing degree under the present climate change scenario. This calls for effective measures to be deployed to increase the level of agricultural production to meet the needs of soaring world population. Application of osmo-protectants and soluble sugars were reported to counter...

Purpose: Salinity stress (SS) is a major problem that adversely affects crop growth and productivity across the globe. Acetic acid (AA) has emerged as an essential chemical to induce plant stress tolerance. However, the role of AA in plants under SS is not well explored. Therefore, the present study was conducted to determine the effect of AA on gr...

Aging mammalian results in impaired bio-functions and neurological disorders. The current study investigated whether whey protein (WP) syrup supplementation may improve age-related changes in diseased brain indicators like tau protein , β-amyloid and α-amylase. The study was carried out in conjunction with immunohistochemistry, histology, and flow...

Heavy metal (HM) contamination is a serious concern across the globe, and in recent times, HMs’ intensity has significantly increased, posing a serious threat to crop growth and productivity. Heavy metals pose serious health issues in humans by entering the human food chains. Therefore, it is direly needed to reduce the effects of HMs on plants and...

Abiotic stresses are one of the significant threats to soybean (Glycine max L.) growth and yields worldwide. Soybean has a crucial role in the global food supply chain and food security and contributes the main protein share compared to other crops. Hence, there is a vast scientific saddle on soybean researchers to develop tolerant genotypes to mee...

Food security is facing a major threat from salinity and there is a need to develop salt tolerant crop varieties to ensure that the demand for food from the world’s increasing population is met. Salinity mostly occurs in arid and semi-arid regions. It may cause many adverse physiological effects on plants, i.e., toxic ion accumulation, disturbed os...

Investigation on natural sources from plants, animals, and microorganisms that produce gums and mucilages goes on increasing day by day to check their pharmaceutical applications. Different mucilages have been studied for their pharmaceutical effects but the use of date palm (Phoenix dactylifera L.) mucilage as a pharmaceutical excipient is still u...

Investigation on natural sources from plants, animals, and microorganisms that produce gums and mucilages goes on increasing day by day to check their pharmaceutical applications. Different mucilages have been studied for their pharmaceutical effects but the use of date palm (Phoenix dactylifera L.) mucilage as a pharmaceutical excipient is still u...

Improvement of salinity tolerance in rice can minimize the stress-induced yield losses. Rice (Oryza sativa) is one of Asia’s most widely consumed crops, native to the subtropical regions, and is generally associated with sensitivity to salinity stress episodes. Salt-tolerant rice genotypes have been developed using conventional breeding methods; ho...

Salt stress is one of the leading threats to crop growth and productivity across the globe. Salt stress induces serious alterations in plant physiological, metabolic, biochemical functioning and it also disturbs antioxidant activities, cellular membranes, photosynthetic performance, nutrient uptake and plant water uptake and resulting in a signific...

Estrogen Receptor α(ERα) is reported to regulate the expression of many target genes by binding to specific estrogen response elements (EREs) in their promoters. c-myc is known to be over-expressed in most of the human carcinomas due to dysregulated transcription, translation, or protein stability. Estrogen (E) can induce the c-myc expression by bi...

Drought stress (DS) is a serious abiotic stress and a major concern across the globe as its intensity is continuously climbing. Therefore, it is direly needed to develop new management strategies to mitigate the adverse effects of DS to ensure better crop productivity and food security. The use of arbuscular mycorrhizal fungi (AMF) has emerged as a...

Diabetes kidney damage (DKD) is a chronic inflammatory disease of the kidney induced with continuous hyperglycemia as the most prevalent consequence of diabetes. Washingtonia filifera seed oil (WFO) was used as a traditional medicine to cure various diseases in ancient Saudi. This work was carried out to investigate the potential protective impact...

Salinity stress (SS) is a major and increasing abiotic stress adversely affecting plant growth and productivity across the globe. The application of organic amendments (OA) is considered to be an important practice to mitigate the adverse impacts of SS. Therefore, this study was performed to assess the impact of different OA on growth, physiology a...

Ramie is one of the most significant fiber crops and contributes to good quality fiber. Drought stress (DS) is one of the most devastating abiotic factors which is accountable for a substantial loss in crop growth and production and disturbing sustainable crop production. DS impairs growth, plant water relation, and nutrient uptake. Ramie has evolv...

Global warming in this century increases incidences of various abiotic stresses restricting plant growth and productivity and posing a severe threat to global food production and security. The plant produces different osmolytes and hormones to combat the harmful effects of these abiotic stresses. Melatonin (MT) is a plant hormone that possesses exc...

We developed alginate-based floating microbeads of clarithromycin with therapeutic oils for the possible eradication of Helicobacter pylori (H. pylori) infections by enhancing the residence time of the carrier at the site of infection. In pursuit of this endeavor, the alginate was blended with hydroxy propyl methyl cellulose (HPMC) as an interpenet...

Weeds are important components of the agroecosystems due to their role as primary producers within the farming systems, yet they are considered as major constraints to crop production. A phytosociological study was conducted to assess the composition and spatial distribution of existing weed species under the influence of various edaphic factors in...

Background Salinity stress (SS) is a serious detrimental factor for crop growth and productivity and its intensity it is continuously increasing which is posing serious threat to global food security. Hydrogen peroxide (H2O2) priming has emerged as an excellent strategy to mitigate the adverse impacts of SS. However, the role of H2O2 priming in mit...

Citation: Ismail, I.A.; Qari, S.H.; Shawer, R.; Elshaer, M.M.; Dessoky, E.S.; Youssef, N.H.; Hamad, N.A.; Abdelkhalek, A.; Elsamra, I.A.; Behiry, S.

Legumes are an imperative source of food and proteins across the globe. They also improve soil fertility through symbiotic nitrogen fixation (SNF). Genome editing (GE) is now a novel way of developing desirable traits in legume crops. Genome editing tools like clustered regularly interspaced short palindromic repeats (CRISPR) system permits a defin...

Heat stress (HS) is a serious environmental stress that negatively affects crop growth and productivity across the globe. The recent increase in atmospheric temperature caused by global warming has increased its intensity, which is a serious challenge that needs to be addressed. Plant growth and development involves a series of physiological, metab...

Hydroxyapatite nanoparticles (HAn) have been produced as biomaterial from biowaste, especially snail shells (Atactodea glabrata). It is critical to recycle the waste product in a biomedical application to overcome antibiotic resistance as well as biocompatibility with normal tissues. Moreover , EDX, TEM, and FT-IR analyses have been used to charact...

The novel coronavirus (2019-nCoV) triggered a worldwide rise in the prevalence of the coronavirus outbreak (COVID-19) and surfaced as a universal wellbeing matter. Analogous with SARS-CoV and MERS-CoV, the main 3-chymotrypsin-alike cysteine protease (3CL Pro) virus enzyme that manages the replications of 2019-nCoV and regulates its existence span,...

The continuous use of pesticides can cause many serious problems in the environmental system. So, it is very necessary to go back to nature again and look for safe alternatives to chemical pesticides. During this research, we evaluated the toxicity of two types of natural substances (wood vinegar and seaweed extract) against three types of zoologic...