Salome Ibietela Douglas

• B.Sc, M. Sc. & Ph.D
• Rivers State University

This study aimed to evaluate the enhanced bioremediation of degreasers: Aquabreak and Teepol polluted wetland soil using Bacillus amyloliquefaciens and Pseudomonas putida. It employs laboratory-scale experimental designs, statistical data and interpretation. The wetland soil samples were collected from Oguruama at latitudes 4o4/45.8// N and longitu...

Oil spills are a significant environmental concern, particularly due to the potential toxicity of the oil dispersants used for remediation. This study investigated the ecotoxicity of the oil spill dispersant Sea Care on Nitrobacter vulgaris and Nitrosomonas halophila which play key roles in the nitrogen cycle. The bacteria were isolated from water...

Aquatic ecosystems have been shown to be adversely affected by pesticides such as Gammalin 20, which can have an adverse effect on primary and secondary producers. This study investigated the ecotoxicity of gammalin 20 on Nitrobacter vulgaris and Nitrosomonas halophila in Freshwater, Marine water and Estuarine water samples collected from Adoni Loc...

This study was aimed at the isolation and molecular characterization of bacteria associated with catfish from culture and wild environments. Samples of gills of Arius heudelotii and Claris gariepinus fishes of economic value from the New Calabar River Choba and earthen ponds from Aluu in Rivers State were collected and analysed. Characterization of...

Microbial investigation of live poultry birds were carried out in three different locations (Aluu, Elioparanwo and RSU) in Ikwerre, Obio-Akpor and Port Harcourt Local Government Areas, Rivers State, Nigeria. In this study a total of 125 samples of muscle (25), breast (25), intestine (25), wing (25) and leg (25) of poultry birds were analysed using...

Phosphate-solubilizing bacteria (PSB) are important in the environment due to their role in the Phosphorus cycle, making fixed phosphorus accessible to plants for their use in producing organic acids that dissolve inaccessible phosphate compounds. This study was aimed to isolate and identify bacteria with phosphate solubilizing potential from soil...

This study was aimed at the isolation and identification of Vibrio spp. associated with Arius heudelotii, a catfish from a wild environment. Twenty-four Arius heudelotii (Catfish) were collected from the New Calabar River Choba, in Obio-Akpor LGA in Rivers State, Nigeria, and were processed to sample their skin, gills, and intestines. The Vibrio sp...

Slum is characterized by uncontrollable development around urban areas, with huge settlements from migrants from the rural areas for greener pastures. This situation is associated with increasing environmental problems predisposing the inhabitants to public health problems. This study was therefore aimed to assess the prevalence and resistance patt...

The use of antibiotics in agriculture is believed to contribute to the spread of antibiotic resistance, and consumption of vegetables represents a route of direct human exposure to resistant bacteria found in soil. This study was carried out to isolate and characterize antibiotic resistant bacteria from poultry dropping fertilized farm soils in Alu...

This study evaluated the microbiology and level of PAH contamination of Pomadasys commersonnii and surface water along Ikpukulu Creek in Borikiri. Standard microbiological procedures were used to assess and identify total heterotrophic bacteria (THB), total fungi and hydrocarbon utilizing bacteria (HUB) and fungi (HUF). PAHs were analyzed using...

Wound infection remains a common and widespread problem contributing to significant morbidity and mortality. It prolongs hospital stay and increases the cost of hospitalization. Morbidity and mortality is partly attributed to increase in infections due to antimicrobial resistant bacteria which make the choice of empirical therapy more difficult. Th...

Yeasts are the most important microorganisms during alcoholic fermentation because they influence fermentation speed, wine flavour and other wine qualities. The aim of this study was to Screen Yeasts Isolated from Agro-Material for Alcohol Fermentation Potential. Agro-materials used were palm wine and pineapple waste. The palm wine was left to ferm...

Aim: The aim of this study was to isolate and characterize the culturable endophytic bacteria from the roots and leaves of three different mangrove plants found growing in same environment. Study Design: This study employs experimental design, statistical analysis of the data and interpretation. Place of Study: The roots and leaves of three mangrov...

Water occupies an important place in the performance of many vital activities such as circulation, excretion, reproduction, and various other metabolic activities that take place in the body. This study aims to assess the bacteriological quality of surface water sources used for domestic purposes by rural communities in Khana Local Government Area...

Aim: The study was aimed to determine the toxicity of paraquat dichloride and lambda-cyhalothrin on phosphate solubilizing bacteria, Pantoa dispersa in aquatic ecosystems. Study Design: This study employs randomized block design, statistical analysis of the data and interpretation. Place and Duration of the Study: Soil sample was collected from the...

The physicochemical properties of water play a major role in evaluating water quality and could serve as a tool to determine the impact of human activities on the water body. Water samples were collected from four different stations with human activities (Abattoir, toilet, jetty, and drinking water points) along Lobia creek and from the fifth stati...

Aim: The aim of this study is to screen and determine the biodegradation potential of the endophytic bacteria isolated from roots and leaves of mangrove plants. Methodology: In this work were isolated, identified and screened endophytic bacteria from roots of Rhizophora mangle, Avicennia germinans and Acrostichum aureum; the three major species of...

Aim: Bacterial biofilm formation is a menacing attribute that has been linked to a rise in antibiotic resistance. The aim of this study was to isolate biofilm-producing bacteria from stool samples and their antibiogram. Study Design: The study involved laboratory research, statistical analysis and interpretation of the data. Place and Duration of S...

The pollution of soil with different types and classes of hydrocarbon resulting from the artisanal refining of crude oil has been the greatest cause of environmental challenges in the Niger Delta. Application of agricultural waste materials as a way of bio-stimulating the indigenous microbiota for the development of sustainable bioremediation proce...

Carbapenem-resistant Enterobacteriacaeae (CRE) are a serious new public-health problem. Carbapenem medicines are a class of antibiotics that are often used to treat severe infections caused by bacteria that are antibiotic-resistant. The goal of this study was to use both conventional and molecular methods to isolate, characterize, and identify Carb...

The study was conducted at three (3) hospitals; University of Port Harcourt Teaching Hospital (UPTH), Meridian Hospital D / line branch (MRD1) and Meridian Hospital Ikoku branch, all located in Port Harcourt, Rivers State. Sample collection was for three (3) months, analysis was carried out daily for six (6) months. A total of Forty-five (45) water...

Aim: The aim of this study was to determine the antibiogram of biofilm producing bacteria isolated from urine of patients in three hospitals in Port Harcourt, Rivers State. Study Design: The study employs statistical analysis of the data and interpretation Place and Duration of Study: The study was conducted at three (3) hospitals; University of Po...

Aim: This study was carried out to compare the bacteriological and physicochemical qualities of boreholes and mono-pumps water samples used as sources of domestic water supply, in Abonnema Community, Akuku-Toru Local Government Area, Rivers State. Methodology: A total of forty-eight water samples were collected twice monthly for four months and eva...

Fish pond wastewater management is one of the problems having the greatest impact on the environment. This untreated fish pond waste water contains organic materials, pathogenic microorganisms, nutrients and toxic compounds, which when discharged into the environment and it runs off into the water bodies in excess could cause algal bloom (eutrophic...

In Nigeria, due to lack of sanitary landfills and general poor attitude towards waste management, various types of wastes such as: commercial, hospital, construction and domestic wastes are mixed and transported in open trucks to unprotected open dumpsites. This is likely to interfere with degradation of biodegradable wastes by non-biodegradable co...

Bakery products such as bread, cakes, etc are staple foods consumed by both the poor and rich. Bread and other bakery products are subject to fungal contamination. This study is aimed at identifying the different fungi associated with bakery products in Port Harcourt metropolis, Nigeria. Sampling was done once monthly for three months, January to M...

Aims: The purpose of this study was to isolate and screen soil fungi that are able to tolerate the contents of spent deep cycle battery (inverter), and to test for their bioremediation potential. Place and Duration of Study: Sample: Department of Microbiology, Rivers State University, between June 2019 and February 2020. Methodology: Soil samples w...

Many substances known to have toxic properties are regularly introduced into the environment through human activity. These substances which include hydrocarbons range in degree of toxicity and danger to human health. Frequent oil spills incidents have become a problem to ecological protection efforts. Conventional methods to remove, reduce or mitig...

Reduced susceptibility of antibiotics against Enterobacterial strains have emerged as an important public health problem worldwide. Infections caused by Escherichia coli and Klebsiella pneumonia can affect severely ill patients, and their colonization of human gut, endangers population at large in communities, and in hospitals. This research is aim...

Comparative evaluation of crude oil degradability efficiency of Bacillus amyloliquefaciens and Comamonas testosteroni with nutrient amendment were investigated in Crude oil Contaminated Soil. The bacteria species used in this study were isolated from the soil collected from Rivers state university research farm using standard microbiological method...

Raffia baskets are used in rural communities to preserve fresh vegetables for a given period of time. This method of storage is aimed at extending the shelf life of the vegetables until they are used. Thus, this study was aimed at determining the microbial succession during storage and susceptibility pattern of bacterial isolates from stored leafy...

Aim: Vegetables are good sources of nutrients such as minerals, oil, vitamins and carbohydrates and are used in preparing different delicacies such as soups and salads. This study was aimed at investigating the microbiological quality and antibiotic resistance pattern of microorganisms associated with stored vegetables in raffia baskets. Methodolog...

Surfactants are a surface-active group of molecular compounds with hydrophobic and hydrophilic moieties in one single molecule that distributes themselves between two immiscible fluids, reduce surface/ interfacial tensions and cause the solubility of non-polar compounds in polar solvents. Besides surface and interfacial activities, they display pro...

Aim: To evaluate the effect of illegal crude oil refining activities on soil microorganisms using standard microbiological methods. Study Design: This study employs laboratory experimental design, statistical analysis of the data and interpretation. Place and Duration of Study: Soil samples were taken once a month for three months (May- July, 2018)...

Indoor air within a hospital building may act as a source of disease causing organisms, which may be transmitted by the patient and could cause airborne or hospital acquired infections. The indoor air of a tertiary health institution in Port Harcourt, Rivers State was investigated to know the level of microbial contamination. The Koch sedimentation...

Aim: To determine and compare the bacterial flora and proximate composition of edible frogs from some selected Local Government Areas (LGA) of Rivers State. Place and Duration of Study: Live adult edible frogs were collected from six locations in six Local Government Areas in Rivers State which includes; Biara (E 7º29’ 2.097’’, N 4º78’ 70.608’’) in...

The tolerance of Nitrosomonas and Nitrobacter species to Carbon furan and Cyahalothrin Pesticides in the soil were investigated. This study employs experimental design and statistical analysis of data and interpretation. Soil samples were collected from the school farm, where there is no history of pesticide application. The toxicity testing was do...

This research investigates the prevalence and antibiotic susceptibility pattern of Staphylococcus aureus in ready-to-eat Corbiculid heterodont in Bayelsa State, Nigeria

ABSTRACT Aim: To analyse and compare the effect of two products of spent phone batteries on bacteria (Pseudomonas sp.) and fungi (Mucor sp.) in marine, brackish and freshwater using standard toxicological bioassay. Study Design: The study employs experimental design, statistical analysis of the data and interpretation. Place and Duration of Study...

This study reports the effect of waste water on biodegradation of drilling fluid contamination in brackish water environment. The experimental set ups include; Control 1(Brackish water with Water base drilling fluid) (BW+WB), Control 2 (Brackish water with Oil base drilling fluid) (BW+OB), Brackish water with Water base drilling fluid with Waste wa...

This study was carried out to assess the microbiological quality of the meat of the frog, Hoplobatrachus occipitalis, which is used as an alternative source of protein (food). Samples were collected from Igwuruta, in Ikwerre Local Government Area of Rivers State, Nigeria. The research work was done to determine if there is any potential risk of foo...

Antimicrobial effectiveness of seven unbranded locally formulated disinfectants sold in some major markets in Port Harcourt, were tested on three clinical bacterial isolates, which includes; Streptococcus pneumoniae, Escherichia coli and Staphylococcus aureus. These isolates were obtained from clinical samples collected from the Medical Microbiolog...

Bacterial and fungal populations in an uncontaminated soil, diesel contaminated soil; taken close to a diesel generator plant and artificially contaminated soil (an uncontaminated soil sample to which diesel was added). These soil samples were analysed to know their microbial population and diversity. Plate counts using plate count agar, mineral sa...

This research was carried out to investigate the presence and occurrence of fungi in three selected pond water samples from Ogoniland, Rivers State Nigeria. Pond water samples were collected monthly for eight months covering both seasons, using Standard procedures for sample collection, isolation and identification. A total of ten fungal genera wer...

A bacteriological study of three ponds in Uegwere Bo-ue community, Khana Local Government Area, of Rivers State, Nigeria were investigated to evaluate the water quality of the ponds. Surface water samples were collected monthly for eight months covering both seasons and evaluated. Bacteriological analyses carried out includes: total heterotrophic b...

Bacteria associated with flies of public health importance in Nigeria are not well known and their ecology is also not well understood. We aim to determine the bacteria associated with flies of waste dump site. Three flies of public health significance were collected from a waste dump site of the Rivers State University of Science and Technology, P...

The microbes associated with the oil palm kernel were investigated. The total culturable heterotrophic bacterial, fungal and coliform counts of kernel with holes, the larvae in the kernel with holes and also the internal of the kernel were analysed using standard microbiological methods. The results of the analysis showed the following genera; Pseu...

Groundwater quality monitoring of some oil locations in the Niger Delta were investigated in order to establish the influence of oil production and storage activities on the groundwater quality of these areas. Water samples were collected from groundwater monitoring boreholes, monthly for twenty four months, during the operational phase of the faci...

The organic quality of some groundwater samples collected around ten flowstations in Niger Delta Nigeria were investigated to demonstrate the effects of oil activities on the organic levels of the water samples. Groundwater samples were collected monthly from the flowstations for twenty four months. Standard analytical techniques as specified by th...