Sally Catt

Sally Catt
Monash University (Australia) · Department of Obstetrics and Gynaecology

PhD, University of Sydney

About

62
Publications
6,540
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832
Citations
Additional affiliations
November 1991 - July 1998
The University of Sydney
Position
  • Research Assistant
Description
  • Research into sex-sorting of sperm in domestic species, setting up sheep IVF lab

Publications

Publications (62)
Article
Podocalyxin (PODXL) is a newly identified key negative regulator of human endometrial receptivity, specifically down-regulated in the luminal epithelium at receptivity to permit embryo implantation. Here, we bioinformatically compared the molecular characteristics of PODXL among the human, rhesus macaque and mouse, determined by immunohistochemistr...
Article
Full-text available
RESEARCH QUESTION What is the impact of the response to COVID-19 on the management of fertility treatments and clinical practice around the world. DESIGN We approached fertility clinic associates around the world. They completed an online survey containing 33 questions focused on the country's response to the COVID-19 pandemic. Known fertility cli...
Conference Paper
Full-text available
Study question What are the effects of the initial COVID–19 response on the management of fertility clinics and clinical practice around the world? Summary answer In the COVID–19 outbreak, the large majority of fertility clinics worldwide suspended fertility treatments. In cycles that continued, there was a shift to frozen embryo-transfer. What i...
Article
Full-text available
Like other assisted reproductive technology (ART) procedures, the cost of egg freezing (EF) is significant, presenting a potential barrier to access. Given recent technological advancements and rising demand for EF, it is timely to reassess how EF is funded. An online cross-sectional survey was conducted in Victoria, Australia and was completed by...
Article
Background Since 2014, many companies have followed the lead of Apple and Facebook and now offer financial support to female employees to access egg freezing. Australian companies may soon make similar offers. Employer-sponsored egg freezing (ESEF) has raised concerns and there is academic debate about whether ESEF promotes reproductive autonomy or...
Article
Full-text available
Purpose Oocyte quality and reproductive outcome are negatively affected by advanced maternal age, ovarian stimulation and method of oocyte maturation during assisted reproduction; however, the mechanisms responsible for these associations are not fully understood. The aim of this study was to compare the effects of ageing, ovarian stimulation and i...
Article
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The Egyptian or Common spiny mouse ( A . cahirinus ) is the first rodent species to show human-like menstruation and spontaneous decidualisation. We consider from these, and its other, human-like characteristics that this species will be a more useful and appropriate small animal model for human reproductive studies. Based on this, there is a need...
Article
STUDY QUESTION What are the cohort trends of women undergoing oocyte cryopreservation (OC)? SUMMARY ANSWER There has been a dramatic increase in OC cycles undertaken each year since 2010, and the demographics of women accessing OC has shifted to a younger age group, but so far very few women have returned to use their cryopreserved oocytes in trea...
Article
The menstruating Egyptian spiny mouse has recently been proposed as a new animal model for reproductive health research. Unfortunately, little is known about reproduction in males. This study compared several characteristics of sperm function before and after cryopreservation. Epididymal spermatozoa were cryopreserved in different concentrations of...
Article
Full-text available
Study question: Does female ageing have a negative effect on the DNA repair capacity of oocytes fertilised by spermatozoa with controlled levels of DNA damage? Summary answer: Compared to oocytes from younger females, oocytes from older females have a reduced capacity to repair damaged DNA introduced by spermatozoa. What is known already: The...
Article
STUDY QUESTION: Is male age associated with the clinical outcomes of IVF/ICSI cycles for idiopathic infertility after adjustment for female age? SUMMARY ANSWER: Male ageing is negatively associated with clinical IVF/ICSI outcomes in couples with idiopathic infertility independent of female age. WHAT IS KNOWN ALREADY: The effect of male age on the...
Article
Bovine in vitro fertilisation technology has been widely exploited in commercial settings. The majority of in vitro-derived cattle embryos are transferred into recipient cows as recently collected (i.e. 'fresh') embryos due to the lack of a reliable cryopreservation method that results in favourable pregnancy rates following transfer of thawed embr...
Article
Full-text available
Mouse and lamb oocytes were vitrified with, or exposed to, different cryoprotectants and evaluated for their effects on their survival and developmental competence after in vitro fertilization (IVF) and activation treatments. Control oocytes remained untreated, whilst the remainder were exposed to three different combinations of vitrification solut...
Article
Sperm cryopreservation protocols are species specific and there is currently no defined cryopreservation protocol for the spiny mouse. The aim of this study was to define a robust cryopreservation protocol for spiny mouse mature, epididymal spermatozoa. Epididymal sperm samples from adult spiny mice (n=24) were cryopreserved at two different rates...
Conference Paper
Study question: Does female aging have a negative effect on oocyte DNA repair capacity of controlled DNA damage levels introduced by spermatozoa? Summary answer: Oocytes from older females have reduced capacity to repair damaged DNA introduced by spermatozoa, compared to oocytes from younger females. What is known already: The reproductive lifespan...
Article
Background: Modifications to in vitro maturation (IVM) media are made to improve rates of blastocyst formation and quality of mammalian embryos. Embryo quality is an important factor in the viability of embryos following cryopreservation. Salubrinal is a specific inhibitor of endoplasmic reticulum stress-induced apoptosis in eukaryotic cells. Here...
Article
Objective: The aim of this experiment was to compare the effect of warming media prepared with or without the inclusion of sucrose on the survival and hatching capacity of vitrified in vitro-derived bovine embryos. Materials and methods: Expanded blastocysts were produced in vitro and vitrified. Vitrified embryos were warmed either successively...
Article
Full-text available
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow‑cooling (18% w/v raffinose, RS‑C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh c...
Article
Full-text available
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh c...
Article
Full-text available
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh c...
Conference Paper
Objective: The aims of this study were i) to compare the effects of different cryopreservation protocols on the functions of mouse epididymal spermatozoa and ii) to examine the effects of vitrification on IVF outcomes and embryo development using epididymal sperm Design: An F1 experimental mouse model was used to provide epididymal sperm samples an...
Article
Full-text available
This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow‑cooling (18% w/v raffinose, RS‑C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh c...
Article
Full-text available
A state of collapse: does this improve blastocyst survival after vitrification? Sally Catt1 , Zuzanna Zejden2 , Malvika Khanna1 , Penny Chen1 , Mulyoto Pangestu1 , Peter Temple Smith1 1 Education Program in Reproduction and Development, Department of Obstetrics and Gynaecology, Monash University, Clayton, Vic, Australia; 2 Division of Infertility a...
Conference Paper
Study question In patients less than 40 years of age, are embryos associated with sero-discordant couples with Hepatitis B or C infections compromised when compared to patients who are sero-negative? Summary answer From our cohort of 60 IVF patients with Hepatitis B or C infections there was no apparent detriment to the quality of the embryos when...
Article
Study question To analyze the impact of consanguinity on the reproductive health of daughters from consanguine parents. Summary answer Significantly reduced ovarian reserve is a likely cause of infertility in daughters of consanguine parents. Hence, in societies with a high rate of consanguinity, consanguinity-related premature ovarian failure mig...
Article
Full-text available
Study question In patients less than 40 years of age, are embryos associated with sero-discordant couples with Hepatitis B or C infections compromised when compared to patients who are sero-negative? Summary answer From our cohort of 60 IVF patients with Hepatitis B or C infections there was no apparent detriment to the quality of the embryos when...
Article
Objective: To undertake a multi-centre study to maximize the number of Makler chambers used. Methods: A total of 15 laboratories participated with 31 Makler chambers. A suspension of latex beads was prepared to a concentration of 20 millions per mL, and 0.5 mL aliquots distributed to each participating laboratory. They measured the concentration on...
Article
Full-text available
Introduction: In order to optimize IVF/ICSI treatments it is important that embryos are scored uniformly by different people working in the same laboratory. Lab to lab standardisation for embryo morphological assessment is important for the comparison of results and for doing research. To minimize the inter observer variability, there is a need of...
Article
Full-text available
Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. In this study, we examined the viability and function of oocytes derived in vitro from pre-antral follicles as an alternative method for restoring fertility. Pre-antral follicles (specified as secondary...
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Full-text available
Ovarian tissue cryopreservation and transplantation can be used to preserve fertility for cancer patients. In this study, we assessed the viability and function of ovarian tissue from adult mice that was cryopreserved by solid surface vitrification or traditional slow-cooling using various in vitro and in vivo techniques, including allotransplantat...
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Follicular dynamics of ovarian tissue varies with species and age. The morphology and distribution of primordial, primary and secondary follicles were examined using mouse and lamb ovarian tissue before and after cryopreservation. Ovarian cortical tissue was processed from mouse (C57BL6J × CBA) and abattoir-sourced lamb ovaries and sliced into frag...
Article
The aim of this study was to improve the quality of ram and boar spermatozoa before and after the processes of 'sex-sorting' by flow cytometry. Three semen diluents are examined, hepes-buffered SOF (HSOF), Beltsville thawing solution (BTS) and phosphate-buffered saline in the absence or presence of Ca2+ and Mg2+ salts (PBS or dPBS), with and withou...
Article
The in vitro and in vivo developmental capabilities and kinetics of in vitro development of embryos derived from adult ewes and from unstimulated (16- to 24-week-old) and hormone-stimulated prepubertal (3- to 5-week-old) ewes were assessed. Cleavage was lower for hormone-stimulated (617/1025; 60.2%) than unstimulated prepubertal (117/169; 69.2%) an...
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Full-text available
In vitro matured adult (Experiment 1) and prepubertal (Experiment 2) ewe oocytes were co-incubated with unsorted or sex-sorted frozen-thawed spermatozoa for 2 to 3 h (short) or 18 to 20 h (long) to determine the effects of reducing the gamete co-incubation time during IVF on subsequent embryonic development in vitro. For oocytes derived from adult...
Article
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The production of embryos from prepubertal lambs is inefficient, partly resulting from the low developmental competence of prepubertal lamb oocytes, and partly because a high proportion of lambs fail to respond to hormone stimulation. The development of a hormone stimulation regimen that all lambs respond to would increase the efficiency of breedin...
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Full-text available
Experiments were conducted to determine the effects of lamb age, hormone stimulation (Experiment 1) and response to stimulation (Experiment 2) on the in vitro production of embryos from prepubertal lambs aged 3-4 and 6-7 weeks of age. For 3-4-week-old lambs, hormone stimulation increased the number of follicles (29.9 +/- 15.3 v. 70.6 +/- 8.2), oocy...
Article
Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh...
Article
Full-text available
The characteristics and functional capacity of ram spermatozoa frozen–thawed prior to and after flow cytometric sorting was assessed after incubation (37 °C; 6 h), in vitro fertilisation (IVF), and transfer of fresh and vitrified in vitro produced embryos. Frozen-thawed spermatozoa from two rams were allocated to four treatment groups: (i) non-sort...
Article
Full-text available
Developments in sperm sexing technology have resulted in the birth of a number of offspring after IVF of oocytes from adult animals (Johnson LA, 2000 An. Reprod. Sci. 60–61, 93–107). The aim of this study was to combine sperm sexing technology with juvenile breeding. Merino lambs, 2–3 weeks (n = 43) were hormone stimulated (Morton KM et al., 2003 P...
Article
Most current protocols of in vitro fertilization in ruminants are based on in vitro maturation of oocytes derived from abattoir material. For application of IVF technology to captive endangered species, however, noninvasive techniques are required which allow repeated collection of oocytes from live females. The aim of this study was to develop a m...
Article
The time course of sperm decondensation, oocyte activation, pronuclear formation and the possible causes of abnormalities after intracytoplasmic sperm injection (ICSI) and in vitro fertilisation (IVF) were examined. Frozen-thawed and pooled fresh semen from three different rams were washed and capacitated for ICSI or IVF. In vitro matured oocytes w...
Article
Two experiments were designed to determine the effects of embryonic sex on the rate of development of in vitro-produced ovine embryos and the effects of transferring early developing ovine blastocysts on the sex ratio of the lambs born. In Experiment 1, in vitro matured and fertilized (IVM/IVF) ovine oocytes were cultured in synthetic oviduct fluid...
Article
Full-text available
Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is still concern over its genetic saf...
Article
Full-text available
Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is still concern over its genetic saf...
Article
Development to the blastocyst stage and survival following embryo transfer were assessed for oocytes obtained from prepubertal and adult sheep matured and fertilized in vitro. The rates of maturation, fertilization and cleavage in vitro did not differ significantly between oocytes from prepubertal and adult sheep. The proportion of cleaved zygotes...
Article
Full-text available
The developmental competence of in vitro matured ovine oocytes, cytoplasmically injected with single male or female chromosome-bearing sperm, was investigated. Eighty-five unsorted, 92 'female-sorted' and 74 'male-sorted' ram sperm were injected into in vitro matured sheep oocytes and, two to four hours later, placed into the oviducts of 28 oestrou...

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Projects (5)
Project
The aim was to assess the effect of male age in IVF/ICSI treatments considering several known confounding factors that impact clinical outcomes
Archived project