Salah Adlat

Salah Adlat
Mayo Clinic | MAYO · Department of Gastroenterology and Hepatology

Ph.D. Genetics

About

37
Publications
23,350
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197
Citations
Introduction
Salah Adlat is currently a Research Fellow at Mayo Clinic, Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, United States. Salah does research on liver cancer. His main research interest is focusing on the hereditary genetics of hepatocellular carcinoma using CRISPR/Cas9 technology.
Additional affiliations
March 2022 - July 2022
University of Pennsylvania
Position
  • Postdoctoral Researcher
March 2021 - January 2022
Guangdong Academy of Medical Sciences and General Hospital
Position
  • PostDoc Position
September 2016 - September 2020
Northeast Normal University
Position
  • PhD Student
Education
September 2016 - July 2020
Northeast Normal University
Field of study
  • Genetics, Gene Editing

Publications

Publications (37)
Article
Full-text available
Background Cancer diagnoses and deaths among the elderly (65 +) are expected to increase significantly over the next decade. Immune checkpoint inhibitors specifically target ICI genes and enhance immune system function. However, poor outcomes may be associated with aging. Methods We downloaded the Genomic Data Commons from the Cancer Genome Atlas...
Preprint
VEGFB has high sequence homology and shares receptor VEGFR1 with VEGFA and PlGF. However, its function is poorly understood. Using VEGFA repression ( Vegfa Down ), VEGFB knockout ( Vegfb −/− ) and VEGFB over expression ( aP2-Vegfb 167 , aP2-Vegfb 186 ) mouse models, we identified VEGFB 167 as a strong promoter of tumor growth. Universal Vegfb inact...
Article
Full-text available
Cancer is caused by abnormal cell growth and metastasis to other tissues. Development of cancers is complex and underlining mechanisms are mostly unknown. Disco-interacting protein 2 homolog B (DIP2B) is a member of Dip2. There have been reports suggesting that Dip2B may participate in tumor growth and development. However, direct link between DIP2...
Article
Full-text available
The brain is the most sensitive organ to hypoxia in the human body. Hypoxia in the brain will lead to damage to local brain tissue. When the blood supply of ischemic brain tissue is restored, the damage will worsen, that is, cerebral ischemia–reperfusion injury. Hydrogen sulfide (H2S) is a gaseous signal molecule and a novel endogenous neuroregulat...
Preprint
Background. BEX family genes are expressed in many tissues and play significant roles in neuronal development. Methods. In this study, a mouse model of Bex2 gene knockout was generated, using the CRISPR-Cas9 system. A fragment of the coding exon was successfully deleted. RT-qPCR confirmed loss of gene expression at the mRNA level. A transcriptomic...
Article
Full-text available
Immune checkpoint inhibitors (ICIs) have revolutionized metastatic melanoma treatment, but our knowledge of ICI activity across age groups is insufficient. Patients in different age groups with advanced melanoma were selected based on the ICI approval time in this study. Patients with melanoma were identified in the Surveillance, Epidemiology, and...
Article
Full-text available
Cardiovascular diseases (CVD) are a significant cause of human health harm. In the past, stem cell therapy was reported to have functional defects, such as immune rejection, tumorigenicity, and infusion toxicity. Exosomes are extracellular vesicles with lipid bilayer membrane structure, containing proteins, lipids, mRNA, miRNA, DNA, and other molec...
Article
Full-text available
Bex2 is well known for its role in the nervous system, and is associated with neurological disorders, but its role in the lung's physiology is still not reported. To elucidate the functional role of Bex2 in the lung, we generated a Bex2 knock-out (KO) mouse model using the CRISPR-Cas9 technology and performed transcriptomic analysis. A total 652 ge...
Article
In this research, atractylenolide II (ATR II) on apoptosis, cell cycle cells via ER pathway in breast cancer (MDA-MB-231 and MCF-7) cells are assessed. The effect of ATR II on cell proliferation was detected by MTT assay. Additional flow cytometry, luciferase, the western blot were performed to detect the signaling pathway cytotoxicity of ATR II. W...
Article
Full-text available
Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip...
Article
Full-text available
Background Many human genetic diseases arise from point mutations. These genetic diseases can theoretically be corrected through gene therapy. However, gene therapy in clinical application is still far from mature. Nearly half of the pathogenic single-nucleotide polymorphisms (SNPs) are caused by G:C>A:T or T:A>C:G base changes and the ideal approa...
Article
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Myxosarcomas are rare malignant tumors of soft connective tissues, classified into various subtypes, including myxoid liposarcoma, myxoid chondrosarcoma, and myxoid leiomyosarcoma. In this study, we proposed to study the demographic, tumor characteristics, and overall survival rate and compared the treatment modalities between these cancers. Patien...
Article
Full-text available
Vascular endothelial growth factor (VEGF) is important for lung development and function but ideal mouse models are limited to decipher the quantitative relationship between VEGF expression levels and its proper development and pathogenesis. Human SPC promoter has been used to faithfully express genes or cDNAs in the pulmonary epithelium in many tr...
Article
Full-text available
Objective: To assess the role of the complement components (C3 and C4) in the pathogenesis and severity of bronchial asthma (BA) among Yemeni patients. Methods: This cross-sectional, clinic-based study was conducted in Sana’a city, Yemen in the period from March to June 2012. It included 100 BA patients, where demographic and clinical data were co...
Article
Objective: To assess the role of the complement components (C3 and C4) in the pathogenesis and severity of bronchial asthma (BA) among Yemeni patients. Methods: This cross-sectional, clinic-based study was conducted in Sana’a city, Yemen in the period from March to June 2012. It included 100 BA patients, where demographic and clinical data were col...
Article
Full-text available
Molecular and anatomical functions of mammalian Dip2 family members (Dip2A, Dip2B and Dip2C) during organogenesis are largely unknown. Here, we explored the indispensable role of Dip2B in mouse lung development. Using a LacZ reporter, we explored Dip2B expression during embryogenesis. This study shows that Dip2B expression is widely distributed in...
Article
Full-text available
Background and objectives: The immunomodulatory potential of mesenchymal stem cells (MSCs) can be regulated by a variety of molecules, especially cytokines. The inflammatory cytokine, TNF-like ligand 1A (TL1A), has been reported as an inflammation stimulator in multiple autoimmune diseases. Here, we studied the effects of TL1A/TNF-receptor 2 (TNFR...
Article
Full-text available
Disco-interacting protein 2 homolog B (Dip2B) is a member of Dip2 family encoded by Dip2b gene. Dip2B has been reported to regulate murine epithelial KIT+ progenitor cell expansion and differentiation epigenetically via exosomal miRNA targeting during salivary gland organogenesis. However, its molecular functions, cellular activities and biological...
Article
Dip2C is highly expressed in brain and many other tissues but its biological functions are still not clear. Genes regulated by Dip2C in brain have never been studied. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, adaptive immune systems of bacteria and archaea, have been recently deve...
Article
Full-text available
Cardiovascular disease (CVD) is a significant cause of death worldwide. Because of its major individual differences in genetic background, pathogenesis, and disease progression pattern, the mortality risk rate remains high following conventional Western medicine diagnosis under current guidelines. Traditional Chinese medicine (TCM) has important mu...
Article
3D N-doped porous graphene aerogel (3D NPGA) was facilely prepared through a one-step hydrothermal reduction by mixing graphene oxide (GO) and p-phenylenediamine with ammonia solution, followed by a freeze-drying process. Then, the AuPd alloy nanoparticles were deposited on the 3D NPGA to synthesize AuPd/NPGA by reduction of HAuCl4 and PdCl2 using...
Article
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Premature senescence of bone marrow-derived mesenchymal stem cells (BMSC) remains a major concern for their application clinically. Hedgehog signaling has been reported to regulate aging-associated markers and MSC skewed differentiation. Indian Hedgehog (IHH) is a ligand of Hedgehog intracellular pathway considered as an inducer in chondrogenesis o...
Article
Full-text available
Excessive fat accumulation causes obesity and many diseases. Previous study demonstrates VEGFB universal knockout induces obese phenotypes including expansion of white adipose tissue, whitening of brown adipose tissue, increase of fat accumulation and reduction in energy consumption. However, roles of VEGFB in adipose tissues are not clear. In this...
Article
Full-text available
Rheumatoid arthritis (RA) is the major autoimmune destructive disease of joints with a complicated pathogenesis. The contribution of tumor necrosis factor‐like ligand 1A (TL1A) in RA pathogenesis, especially on fibroblast‐like synoviocytes (FLS), has been suggested clinically. The present study investigated the role of TL1A in mitochondrial dysfunc...
Article
Full-text available
Disconnected interacting protein 2 homolog A (DIP2A) is highly expressed in nervous system and respiratory system of developing embryos. However, genes regulated by Dip2a in developing brain and lung have not been systematically studied. Transcriptome of brain and lung in embryonic 19.5 day (E19.5) were compared between wild type and Dip2a-/- mice....
Preprint
Full-text available
Disconnected interacting 2 homolog A (DIP2A) gene is highly expressed in nervous system and respiratory system of developing embryos. However, genes regulated by Dip2a in developing brain and lung have not been systemically studied. Transcriptome of brain and lung in embryonic 19.5 day (E19.5) were compared between wild type and Dip2a-/- mice. Tota...
Article
Full-text available
Prostate cancer is the most common illness affecting men worldwide. Although much progress has been made in the study of prostate cancer prevention and treatment, less attention has been paid to the molecular mechanism of the disease. The molecular arrangement by which atractylenolide II (ATR II) induces human prostate cancer cytotoxicity was compr...
Article
Full-text available
Diagnosis of ANCA associated vasculitides (AAVs) represents a big burden in the medical field because of clinical features diversity. Wegener’s granulomatosis or granulomatosis with polyangiitis (GPA), Churg-Strauss syndrome or eosinophilic granulomatosis with polyangiitis (EGPA), and microscopic polyangiitis (MPA) are the main small-vessel vasculi...
Article
Full-text available
Obesity is the results of excessive energy accumulation and associated with many diseases. We previously reported that universal repression of vascular endothelial growth factor (VEGF) leads to brown-like adipocyte development in white adipose tissues and mice are resistant to obesity. Using an adipose-specific VEGF repression mouse model (aP2-rtTR...
Article
Prostate cancer is a serious affliction worldwide. Although much progress has been made in the study of prostate cancer prevention and treatment, less attention has been paid to the molecular mechanism of the disease. The molecular arrangement by which Schisandrin B (Sch B) induces human prostate cancer cytotoxicity was comprehensively examined in...
Article
Full-text available
Objectives: This study aimed to measure lipoprotein (a) [Lp(a)], lipid profile and mean platelet volume (MPV) in hypothyroid patients compared to healthy controls. Methods: An unmatched case-control study was conducted involving 100 subjects (50 overt hypothyroidism patients and 50 healthy controls admitted to the University of Science and Technol...

Questions

Questions (3)
Question
I would like to know how to get all possible DNA sequences that encode every 20 amino acid (aa means 60nt) frame in protein X and all possible aa substitutions at each residue of this protein.
I want every 20 amino acids in protein X to be linked with 10nt-barcode (huge library, 20 power20) and then test the expression of this protein using high-throughput sequencing. I would like first to get a few sequences of this system. If this works, I will order libraries that combine barcodes-10nt with protein X-60nt and then test the whole library expression using deep sequencing.
The sequence I want looks like this before cloning it to the vector:
NNNNN-[Barcode]--XXX--protein X-60nt(20 aa)]-NNNNN
XXX are 3 restriction endonucleases.
Please help me out with any available tools or websites to do this.
Thank you
Question
Hi,
Could anyone suggest free software or websites used to generate unique DNA barcode sequences [5-10nt] to label XXX genes for library screening?
Thank you in advance

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