Sako Mirzaie

Sako Mirzaie
University of Toronto | U of T

About

49
Publications
12,841
Reads
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593
Citations
Additional affiliations
January 2008 - September 2015
Islamic Azad University of Sanandaj
Position
  • Professor (Assistant)
September 2007 - present
Islamic Azad University of Sanandaj
Position
  • Professor (Assistant)
September 2007 - present
Islamic Azad University
Position
  • Professor (Assistant)

Publications

Publications (49)
Article
Anthrax is a deadly disease caused by Bacillus anthracis, a dangerous biological warfare agent employed for both military and terrorist purposes. A critical selective target for chemotherapy against this disease is nucleoside hydrolase (NH), an enzyme still not found in mammals. In the current study, we have performed molecular docking and dynamics...
Article
D-Pen2,D-Pen5 enkephalin (DPDPE) is one of the most selective synthetic peptide agonist targeting the δ-opioid receptor. Three cyclic analogues of DPDPE containing a xylene bridge in place of disulphide bond have been synthesized and fully characterized as opioid receptors agonists. The in vitro activity was investigated showing a good affinity of...
Article
Development of highly concentrated formulations of protein and peptide drugs is a major challenge due to increased susceptibility to aggregation and precipitation. Numerous drug delivery systems including implantable and wearable controlled-release devices require thermally stable formulations with high concentrations due to limited device sizes an...
Article
Full-text available
Recently, a novel coronavirus initially designated 2019-nCoV but now termed SARS-CoV-2 has emerged and raised global concerns due to its virulence. SARS-CoV-2 is the etiological agent of "coronavirus disease 2019", abbreviated to COVID-19, which despite only being identified at the very end of 2019, has now been classified as a pandemic by the Worl...
Preprint
Background: FAM83H has been identified as an essential gene for dental enamel formation and may be related to Wnt/β-catenin. Methods and Results: the Fam83h -KnockOut mice recruited in this study confirmed by Sanger sequencing and western blot analysis. levels of Fam20a, Dspp, Dmp1, Enam, Ambn, Sppl2a, Mmp20, Fgf10 , and the mediators of Wnt/β-cate...
Article
Background: Mutations in bacteria frequently occur that display a crucial need for new antimicrobial agents. Metallo-β-lactamases (MBLs) are a growing threat to maintaining the effectiveness of beta-lactam antibiotics. Resistance to beta-lactam antibiotics is one of the most common types of antibiotic resistance, which causes the ineffectiveness of...
Article
Full-text available
Objective Membrane type-matrix metalloproteinases (MT-MMPs) are known as key regulators of cancer progression/metastasis. However, their roles in the growth and progression of multiple myeloma (MM) have not been yet elucidated. Methods and materials The expression of 6 MT-MMPs in MM, B cell lines, and normal peripheral blood (PB) cells were measur...
Article
Full-text available
The kappa opioid receptor (KOR) represents an attractive target for the development of drugs as potential antidepressants, anxiolytics and analgesics. A robust computational approach may guarantee a reduction in costs in the initial stages of drug discovery, novelty and accurate results. In this work, a virtual screening workflow of a library consi...
Chapter
Nowadays, many viral infections have emerged and are taking a huge toll on human lives globally. Meanwhile, viral resistance to current drugs has drastically increased. Hence, there is a pressing need to design potent broad-spectrum antiviral agents to treat a variety of viral infections and overcome viral resistance. Covalent inhibitors have the p...
Article
Hypoglycemia is a serious and potentially fatal complication experienced by people with insulin-dependent diabetes. The complication is usually caused by insulin overdose, skipping meals, and/or excessive physical activities. In type 1 diabetes (T1D), on top of impaired pancreatic α-cells, excessive levels of somatostatin from δ-cells further inhib...
Preprint
Full-text available
Background: Clove oil is known for its medicinal properties. The mechanism of anti-cancer properties of Syzygium aromaticum were investigated by mathematical modelling on the genome scale with metabolomics using 1H Nuclear Magnetic Resonance spectroscopy on Raji cells. Objectives: An integrative analysis correlated the metabolites identified by...
Article
Full-text available
Folate antagonists are classified as important and valuable therapeutic agents against infection, neoplastic, and inflammatory diseases. Dihydrofolate reductase (DHFR) is a biological target of two well-defined folate antagonists, classical and non-classical inhibitors. DHFR catalyzes the reduction of 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate b...
Article
The abuse and overuse of antibiotics is the main responsible for the raising antibiotic resistance among bacteria and as a consequence the of diseases severity and treatment complications. Staphylococcus aureus (S. aureus) can cause severe bloodstream infection, endocarditis and skin infection with an annual incidence of twenty-six cases per one hu...
Article
Matrix metalloproteinases (MMPs) play important roles in cancer progression and, despite their inhibitors have failed in the clinical trials, they have always been considered as suitable targets for the treatment of tumor. We have recently shown that membrane type (MT) 2-MMPs, is selectively expressed in multiple myeloma (MM) cells and mediates the...
Article
Brucellosis has always been a threat to the health and economics of societies. We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibo...
Article
In this work we report the application of the Ring-Closing Metathesis (RCM) to the preparation of two cyclic olefin bridged analogues of biphalin (Tyr-D-Ala-Gly-Phe-NH-NH<-Phe<-Gly<-D-Ala<-Tyr), using the second generation Grubbs’ catalyst. The resulting cis and trans cyclic isomers were identified, fully characterized and tested in vitro at μ (MOR...
Article
Full-text available
Family with sequence similarity 83 member H (FAM83H) protein‐coding geneplay an essential role in the structural organization, calcification of developing enamel, and keratin cytoskeleton disassembly by recruiting Casein kinase 1 alpha (CSNK1A1) to keratin filaments. In this study, we have applied CRISPR Cas9 nickase (D10A) to knockout (KO) the Fam...
Article
Pancreatic Lipase (PL) is a key enzyme in the degradation of triglycerides assumed in the diet and it is involved in several pathologies, such as acute pancreatitis, an inflammatory state of pancreas. In this work we performed the virtual screening of a tripeptide library, with the aim to discover novel lead compounds as inhibitors of human PL. The...
Article
Type II l‑asparaginase (l‑ASNase) is an FDA approved enzyme drug with extensive applications for treatment of certain blood cancers. However, the therapeutic efficiency of this enzyme is hampered by its undesirable glutaminase activity. Given the pivotal role of this enzyme against cancer, designing engineered mutants with diminished glutaminase ac...
Article
The influenza H1N1 virus is the causative agent of the flu pandemic in the world. Due to the shortage of effective means of control, it is remained the serious threats to public and avian health. To battle the surge of viral outbreaks, new treatments are crucially needed. The viral RNA polymerase, which is responsible for transcription and replicat...
Article
Full-text available
Introduction: The increasing drug resistance of bacteria against different antibiotics is a cause of development of antimicrobial compounds. The aim of this study was to investigate the synthesis and evaluation of antibacterial effects of 2-amino-5-nitrothiophene derivatives against Staphylococcus aureus and Escherichia coli. Strains. Materials &...
Article
Ursolic Acid (UA), a pentacyclic triterpenoid compound, plays a vital role in aging process. However, the role of UA in the regulation of aging and longevity is still controversial as we have previously demonstrated that UA increases SIRT1 protein level in aged-mice. Here, we reveal that UA directly activates SIRT1 in silico, in vitro and in vivo....
Article
L-Asparaginases (ASNase) belong to a family of amidohydrolases, have both asparaginase and glutaminase activity. Acute lymphocytic leukemia (ALL) is an outrageous disease worldwide. Bacterial ASNase has been used for the treatment of ALL. Glutaminase activity of enzyme causes some side effect and it is not essential for anticancer activity. The aim...
Article
Full-text available
In this paper we reported the synthesis and the in vitro and the in vivo biological evaluation of linear pseudo-peptides incorporating the N-VGCC blocker tripeptide Phe-NMe-Leu-Tyr(OBz)-NtBu and the biphalin pharmacophore Tyr-d-Ala-Gly-Phe. The novel sequences have been designed by using amino acids of different length to join the two pharmacophore...
Article
Ipomoea batatas L. is widely used as a functional food in many countries. In this work, I. batatas leaf extracts (Soxhlet extract, microwave [MW] extract and decoction extract) were characterized for the first time, and their total flavonoid and phenolic compound contents were measured by spectrophotometric and chromatographic analyses. These extra...
Article
Full-text available
Background: In recent years, the multi-drug resistance of bacteria and fungi strains has become a worldwide problem. The incidence of fungal and bacterial infections is experiencing a serious growth and the microorganisms have developed several new mechanisms of antibiotic resistance. Methods: Antimicrobial peptides (AMPs) constitute one of the wel...
Article
Recently, the quick spreads of broad-spectrum beta-lactams antibiotic resistance in pathogenic strains of bacteria have become a major global health problem. These new emerging resistances cause ineffectiveness of antibiotics and increasing the severity of diseases and treatment costs. Among different and diverse resistance targets, we chose a clas...
Article
Homotetrameric β-xylosidase from Selenomonas ruminantium (SXA) is one of the most efficient enzymes known for the hydrolysis of cell wall hemicellulose. SXA shows a rapid rate of activity loss at temperatures above 50 °C. In this study, we have introduced two inter-subunit disulfide bridges with one point mutation. Lys237 was chosen to be replaced...
Article
Full-text available
The endocannabinoid system is activated by the binding of natural arachidonic acid derivatives (endogenous cannabinoids or endocannabinoids) as lipophilic messengers to cannabinoid receptors CB1 and CB2. The endocannabinoid system comprises also many hydrolytic enzymes responsible for the endocannabinoids cleavage, such as FAAH and MAGL. These two...
Conference Paper
Full-text available
امروزه نارسایی در جریان خون یکی از دلایل قابل توجه مرگ و میر در جهان شناسایی شده است که در پی ایجاد لخته های خونی در عروق اتفاق می افتد و باعث بروز سکته های قلبی، مغزی و آمبولی ریوی می شود. سیستم فیبرینولیتیک در بدن از گسترش لخته ی تشکیل شده در جریان خون جلوگیری می کند و مکانیسم لیز شدن لخته با فعال شدن پیش آنزیم پلاسمینوژن بافتی آغاز می شود که یک...
Article
The human immunodeficiency virus (HIV) which is strictly related to the development of AIDS, is treated by a cocktail of drugs, but due its high propensity gain drug resistance, the rational development of new medicine is highly desired. Among the different mechanism of action we selected the reverse transcriptase (RT) inhibition, for our studies....
Article
Fentanyl is a powerful opiate analgesic typically used for the treatment of severe and chronic pain, but its prescription is strongly limited by the well-documented side-effects. Different approaches have been applied to develop strong analgesic drugs with reduced pharmacologic side-effects. One of the most promising is the design of multitarget dr...
Article
The dipeptide aspartame (Asp-Phe-OMe) is a sweetener widely used in replacement of sucrose by food industry. 2′,6′-Dimethyltyrosine (DMT) and 2′,6′-dimethylphenylalanine (DMP) are two synthetic phenylalanine-constrained analogues, with a limited freedom in χ-space due to the presence of methyl groups in position 2′,6′ of the aromatic ring. These re...
Article
Unnatural aromatic amino acids are useful tools in drug discovery, since their insertion in bioactive peptide sequences can change the side chains spatial orientation, the backbone conformation and above all, their bioactivity. In this communication, we propose a straightforward method to synthesize 2′,6′-dimethyl-tyrosine and 2′,6′-dimehylphenyl-a...
Article
The effects of different monoterpenes and 2- cyclohexen-1-one on the antibacterial activity of nitrofurantoin against resistant Enterobacter cloacae, were compared and the minimal structural component of monoterpene required for the highest level of resistance-modulating activity was determined. Subinhibitory concentrations of all compounds tested...
Article
Full-text available
Oxidation of low-density lipoprotein (LDL) has been strongly implicated in the pathogenesis of atherosclerosis. The use of some compound in dietary food stuff include Myrtle (Myrtus communis L.) leaves hydroalcoholic extract (ME) may lead to the inhibition of production of oxidized LDL and may decrease both the development and the progression of at...
Article
Full-text available
Purpose: Accumulating evidence indicates that GZ and its hydrolyzed metabolite 18-β glycyrrhetinic acid (GA) exhibit anti-inflammatory and anticancer activities. The objective of this study was to examine the in vitro cytotoxic activity of GA on human ovarian cancer A2780 cells. Methods: A2780 cells were cultured in RPMI1640 containing 10% fetal bo...
Article
Full-text available
Acute leukemia is characterized by the accumulation of neoplastic cells in the bone marrow and peripheral blood. Currently, chemotherapy and differentiating agents have been used for the treatment of leukemia. Recently, plant extracts, either alone or in combination with chemo agents, have been proposed to be used for the treatment of cancers. The...
Article
Abstract Resistance to antibiotics in bacteria, is one of the major problems of mankind. Each year, a large number of patients due to infection, lose their lives. One of the main mechanisms of antibiotic resistance, is beta-lactamase secretion. This enzyme, hydrolyzes the amide bond of a lactam ring in beta-lactam antibiotics. Bacillus licheniformi...
Conference Paper
Full-text available
Background: CXCR4 is a G protein-coupled receptor (GPCR) that has multiple critical functions in normal and pathologic physiology that include regulation of the metastatic behavior of mammary carcinoma, utilization as a co-receptor for infection by T-tropic strains of human immunodeficiency virus-1 and mobilization of stem cells from bone marrow to...
Article
Trastuzumab (Herceptin(®) ) is a monoclonal antibody (mAb) for specific ablation of HER2-overexpressing malignant breast cancer cells. Intensification of anti-proliferative activity of trastuzumab through construction of immunotoxins and nano-immunoconjugates is a promising approach for treatment of cancer. In the current study, trastuzumab was dir...
Article
Full-text available
Inhibition of aromatase (CYTP450) as a key enzyme in the estrogen biosynthesis could result in regression of estrogen-dependent tumors and even preventing the promotion of breast cancer. Although today potent steroid and non-steroid inhibitors of aromatase are available, isoflavanone derivatives as natural compounds with least side effects have bee...
Article
Full-text available
A series of new quinazoline derivatives has been recently reported as potent multi-acting histone deacetylase (HDAC), epidermal growth factor receptor (EGFR), and human epidermal growth factor receptor 2 (HER2) inhibitors. HER2 is one of the major targets for the treatment of breast cancer and other carcinomas. Three-dimensional structure-activity...
Article
Monoterpenes from the essential oils of several plants have been shown to enhance the bactericidal activities of nitrofurantoin and furazolidone against the bacteria of Enterobacteriaceae family. In this study, computer-aided molecular modeling and docking techniques have been employed to simulate the theoretical mode of interaction between monoter...
Article
The enhancement of the antibacterial activity of ampicillin by different extracts of Berberis integerrima fruits was evaluated against Staphylococcus aureus. Disk diffusion and agar dilution methods were used to determine the antibacterial activity of ampicillin in the absence and presence of different plant extracts or various fractions eluted by...
Conference Paper
1-methyl malate is one of the important organic compound in Berberis integerrima fruits that enhances the antibacterial activity of ampicillin against Staphylococcus aureus [1]. The crystal structure of Staphylococcus aureus β-lactamase (PDB ID: 3BLM) was selected for prediction of binding mode of 1-methyl malate to class A lactamase. In this study...
Article
Full-text available
The effect of nicotine on the regulation of carotenoid production by Dunaliella salina was investigated. Accumulation by the microalga of lycopene, all-trans and 9-cis β-carotene was assessed in artificial sea water containing different concentrations of nicotine. Nicotine inhibits lycopene cyclase, and consequently the cyclization reactions in the...

Questions

Questions (6)
Question
Hi all,
I saw a 3D image from a paper published in "Nature Communications" (Figure is attached).
I want to produce an image like that in my project. Any help is appreciated.
Best regards,
Question
I have purified some hydrolysis enzymes such protease, lipase and so on. I need a best formulation to maintain their stability in solutions. The enzymatic solution will be prepared to clean the medical instruments (like endoscope).
Thank you in advance
Question
Dear All,
I am working on the improvement of streptokinase activity through in silico studies. streptokinase is an enzyme and used as a thrombolytic medication. It will be nice if a research group can help us with its relevant experimental tests to confirm our modeling data.
Best regards,
Question
Dear All,
I am working on the improvement of streptokinase activity through in silico studies. streptokinase is an enzyme and used as a thrombolytic medication. It will be nice if a research group can help us with its relevant experimental tests to confirm our modeling data.
Best regards,
Question
Dear all,
I want to powder an insect, a Locust, for use in animal feeding. How can I make an eatable powder from it?
Question
I want to buy a system to run the molecular dynamics of proteins by gromacs. Now, I have two options to buy the best system. which system could be faster for my MD analysis?
system A: COY intel xeon e1226 3.3GHz, 8mb cashe, 32gb DDR3 RAM, HDD 2TR, power 250r.
system B: Mainboard : ASUS - X99-A NEW - Socket 20 11 Series
cpu : Intel Core i7 5820 K
RAM : G.SKILL Ripjaws 4 series 16GB (4 x 4GB) 288-Pin 2666 (PC4-21300) CL 15 RED
Vga : geforce g 730 4G Real
H.d.d : Western Digital 1TB 64 Mb Buffer 7200 RPM
Power : cable manage 630 W Real Redmax Dual Cooling Fan
DVD RW : Asus 24X
case : optional Cable Manage ( DEEP COOL Pro Series )
best regards,

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Projects

Project (1)
Project
Site-directed mutagenesis in Escherichia coli L-asparaginase gene to reduce its glutaminase activity via experimental and molecular modeling methods