Sakharov B.V.

Sakharov B.V.
State Research Center for Applied Microbiology & Biotechnology · Biophysical

About

51
Publications
4,565
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
86
Citations
Introduction
Skills and Expertise

Publications

Publications (51)
Article
Full-text available
In the oil industry, there is a need for fast and non-invasive methods for determining the bitumen content and group composition in rocks samples. Determination of bitumen saturation of reservoir or source rocks has some difficulties due to existing methods limitations. For this purpose, the novel method is based on a combination of free induction...
Article
This work was conducted to discover ways of processing high-paraffin oil residues using thermochemicalprocesses to obtain binders. The principal purpose of such treatment of high-paraffinic residues is in thethermocatalytic process which consists of dehydroesterification via oxidation of paraffins which increasebitumen’s low-temperature stability (...
Article
In this study a new, fast and simple method based on low field NMR-relaxometry for in-situ determination of SARA (saturates, aromatics, resins and asphaltenes) composition of crude oils was proposed. This method was tested on 22 samples of crude oils with a wide range of SARA-composition and API gravity values (4.6-42.0 •), including extra-heavy, h...
Article
Full-text available
The work of many researchers is devoted to studying the problem of the features of the formation of the Bazhenov suite and organic matter. Various methods are used to study the type and state of organic matter, despite this, the problem associated with the phase state and type of organic matter in these deposits, the methods for studying those rema...
Article
Full-text available
The study of the organic matter of Riphean-Vendian deposits, the features of its distribution are the subject of research by many authors. The problem of hydrocarbon of these deposits remains relevant. We have conducted studies of deep-seated rocks of the Lower Riphean of the kaltasinskaya suite of the Eastern-Askinskaya area, with an increased con...
Article
Full-text available
For the analysis of heavy oils, the method of simultaneous measurement of the free induction decay (FID) together with the decay of the echo signal in the Carr-Purcell-Meiboom-Gill (CPMG) pulse program was used. The measurements were carried out on a «Chromatek-Proton 20M» NMR analyzer operating at a frequency of 20 MHz. A special control program w...
Article
Full-text available
For the analysis of heavy oil samples of the Ashalchinskoye deposit the combined nuclear magnetic resonance (NMR) method to measure the free-induction decay (FID) together with the Carr-Purcell-Meiboom-Gill (CPMG) echo signal’s decay was used. The measurements were carried out on a NMR analyzer Chromatek-Proton 20M, operating at a frequency of 20 M...
Conference Paper
Full-text available
Мониторинг свойств сырых тяжёлых нефтей методами магнитной радиоспектроскопии /В.Я.Волков, Б.В.Сахаров, Н.М.Хасанова // Горизонтальные скважины и ГРП в повышении эффективности разработки нефтяных месторождений: Материалы научно-практической конференции.-Казань: Изд-во «Слово», 2017.-149-152 с. International oil conference "Gorizontal'nyye skvazhiny...
Article
Full-text available
В настоящее время групповой анализ сырой нефти проводится с помощью хроматографических методов, таких как SARA (Saturates-Aromatics-Resins-Asphaltenes) [1]. Эти методы трудоёмки, требуют высококвалифицированного персонала, как правило, дороги и небезопасны. Кроме того, хроматографические методы не могут быть использованы для оценки нефтяных компоне...
Conference Paper
Full-text available
Продолжены исследования, направленные на создание метода оценки группового состава тяжёлых нефтей методом ЯМР релаксации с помощью разработанной ранее комбинированной импульсной последовательности ССИ+КПМГ. Показана возможность определения асфальтенов непосредственно в сырой нефти in situ без осаждения н-гептаном и длительной процедуры выделения и...
Conference Paper
Full-text available
Joint measurement and combined processing of FID and echoes in a series of CPMG for assess component composition of heavy oils B.V.Sakharov, N.M.Khasanova, V.Y.Volkov VI AlAll-Russian Conference "New advances in NMR structural studies" Kazan, Kazan Federal University, 2015, p. 67-68
Conference Paper
Full-text available
The pulse sequence type CPMG with different time intervals between 180° pulses for investigating of heterogeneous systems. V.Y.Volkov, E.V.Sosunova, B.V.Sakharov. VI All-Russian Conference "New advances NMR in structural studies" Kazan, Kazan Federal University, 6-9 April 2015, p.37-39. Carr-Purcell-Meiboom-Gill pulse sequence 90°(-t-180°-t-)n wide...
Data
Full-text available
The measurement of parameters such as fat, protein and moisture is important to help define the nutritional value and quality for many foodstuffs and agriculture products. ISO LFNMR standards for food products are based on measurement of FID and echo at a strictly fixed times and need of reference samples. Much useful information which is contained...
Poster
Full-text available
This method reduces the total number of 180° pulses and avoids the ”heating” of the sample and the ”saturation” of the spin system by lowering the RF power absorbed by the sample during the experiment. The method improves the accuracy of determining the relative amplitudes of the relaxation components as the effects of the instability the resonance...
Article
Full-text available
Volkov V.Y., Kleimenov М.А., Sakharov, B.V.,Vikharev Y.А., Burashev V.V., Shabalin P.A. NMR relaxometer-analyser «Proton 20M». Part 2. New opportunities for process control and research Abstract. Method for measuring amplitudes of the FID and the echo signals in a single cycle of multi-pulse sequences like KPMG with increasing intervals between 1...
Article
Full-text available
Volkov V.Y., Kleimenov М.А., Sakharov, B.V.,Vikharev Y.А., Burashev V.V., Shabalin P.A. NMR relaxometer-analiser «Proton 20M». Part 1.Quantitative analysis without the f-factor Abstract. Provides a method for determining the initial amplitudes of the free induction decay’s (FID’s) components immediately after the 900- pulse by fitting the complet...
Chapter
Full-text available
NMR RELAXATION METHODS IN THE FOOD INDUSTRY Volkov, V.Ya. Doctor of Chemical Sciences, Professor Sakharov BV. Head of Radiospectroscopy Lab SRCAMB (State Research Center for Applied Microbiology and Biotechnology) Obolensk (Moscow region). A brief overview of the NMR relaxation method for the analysis of food raw materials and food, as well as for...
Conference Paper
Full-text available
Sakharov B.V., Volkov V.Ya, Zagainov V.F., Shabalin P.A. "Ensuring the uniformity of measurements as an essential element of international cooperation of the CIS countries in the field of Health" pp. 238-239
Article
Collagen spicules from blue shark fin rays were studied by the NMR relaxation method (curves of free induction decay and transversal magnetization decay). The curves of transversal magnetization decay (the Carr–Parcell–Meibum–Gill method) in partly dried samples were shown to be described by the sum of three exponential components ( ~ 151 ms, ~...
Article
Full-text available
The present research involves the development of approaches of the NMR relaxation method for studying the possibility of analyzing the macromolecular components in dry blends of two complementary polymers or in powders of their inter-polymeric complexes. The investigation was carried out on polyethylene glycol (PEG), polymethacrylic acid (PMAA) (M....
Article
Full-text available
NMR-relaxation methods (T1 and T2) were used to get information on molecular dynamics of polyacrylic and polymethacrylic acids in deuterated aqueous solutions including complex solutions with polyethylene oxide. An analysis of structural transitions (at different temperatures) for polymer components at different ionization degrees of polyacids made...
Article
Full-text available
The residual water and dry matter condition in the lyophilized biomass of the yeast Saccharomyces cerevisiae was studied by NMR-relaxation technique. It was shown that the slow component of the transverse magnetization NMR signal spectrum corresponding to the so-called "isolated mobile water" was caused in fact by the interaction of the disaccharid...
Article
Full-text available
The residual moisture and dry matter content of lyophilized biomass of the yeast Saccharomyces cerevisiae were investigated by means of NMR relaxation techniques. It was shown that the slow component NMR transverse magnetization signal, corresponding to the so-called isolated water, was in fact caused by the interaction of the disaccharide trehalos...
Article
Full-text available
Методами ЯМР-релаксации исследовано состояние остаточной влаги и сухого вещества в лиофилизированной биомассе дрожжей Saccharomyces cerevisiae. Показано, что медленная компонента огибающей сигнала ЯМР поперечной намагниченности, соответствующая так называемой изолированной подвижной воде, в действительности обусловлена взаимодействием дисахарида тр...
Article
The present paper reports the results of evaluation of the reversibility of cryodamage to erythrocyte membranes obtained by this method using two variants of inserting the paramagnetic into the system - before and after freezing.
Article
Full-text available
This paper presents the results of the assessment of reversibility cryodamage erythrocyte membranes, which have been obtained by this method, using two versions of the paramagnetic material input into the system - before and after freezing
Article
Full-text available
The dependence of the growth rate of F. tularensis on the osmotic properties of the medium can be presented as a curve with the maximum in the area of 500-600 mOsm. Under these circumstances the intracellular osmotic pressure exceeds the extracellular one by 50-100 mOsm. With the rise of the osmotic pressure in the medium the increase of the concen...
Article
Full-text available
It is shown that diffusive water permeability of E. coli cell membranes at 4-24 degrees C is in the range from 16.6 to 35.0 microM.s-1, that is close to erythrocyte membrane water permeability, but higher than that of lipid bilayers. Cryoprotectants (DMSO and thiourea) at the concentration of 1.0 M considerably decrease water bacterial membrane per...
Article
The permeability of E. coli cell coats for the diffusion of water has been measured by the n.m.r. relaxation method. It is shown that in the 4-24 °C interval it is 16·6-35·0 μm·sec -1 which is close to the water permeability of erythrocyte membranes and considerably higher than that of lipid bilayers. It has been established that cryoprotectors (DM...
Article
Amount of inter--and extracellular unfrozen water in the intact human erythrocytes as well as tau a, "a life time" of water molecules in these cells, have been determined. The progressive compression of the erythrocytes during freezing them as deep as -5 degrees C has been found to submit to the ideal osmometer law. The membrane impermeability to M...
Article
The n.m.r. relaxation method in the presence of paramagnetic Mn 2+ ions in the extracellular medium has been used to determine the quantity of intra- and extracellular unfrozen water in intact human erythrocytes over the temperature range from -1 to -9 °C and also the "lifetime" (τ a) of the water molecules in these cells. It was established that t...
Article
The effects of hypertonic solutions and slow freezing to different temperatures in the range of 0 to -80 degrees C on the permeability of plasma membranes of reconstituted erythrocytes were investigated. It has been found that even increased concentrations of NaCl or KCl (1.2 to 4.2 M) do not change appreciably release of K+, [14C]sucrose and haemo...
Article
Full-text available
Summary: The effect of high salt concentrations and slow freezing to different temperatures in the range of 0 to -80°C on the permeability of reconstituted erythrocytes for hemoglobin and 14C sucrose was studied. It has been found that even highly concentrated solutions of NaCl or KC1 (0.6 to 4.2M) increase leakage of 14C sucrose and K+ , but insig...

Network

Cited By

Projects

Projects (3)
Project
Development of technological bases for the processing of high-paraffinic vacuum residues by thermocatalytic methods to obtain high-quality bitumen.N
Project
Development of the Low Field NMR method for simultaneous analysis of asphaltenes, resins and lighter fractions of heavy oils in situ