Saidhbhe L. O'Riordan

Saidhbhe L. O'Riordan
California Institute of Technology | CIT · Division of Biology

PhD Neurochemistry

About

11
Publications
7,319
Reads
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201
Citations
Introduction
Dr. Saidhbhe L. O'Riordan currently works in the Division of Biology & Biological Engineering (BBE) at California Institute of Technology (Caltech). Saidhbhe's research areas are Biochemistry, Analytical Chemistry and Neuroscience. Saidhbhe's most recent publication is 'Determining the pharmacokinetics of nicotinic drugs in the endoplasmic reticulum using biosensors.'
Additional affiliations
March 2017 - present
California Institute of Technology
Position
  • PostDoc Position
November 2015 - November 2016
Maynooth University
Position
  • Lecturer
Description
  • Neuroscience, Biosensors, Neurochemistry
October 2013 - February 2015
Royal College of Surgeons in Ireland
Position
  • PostDoc Position

Publications

Publications (11)
Article
Loss-of-function mutations in the angiogenin (ANG) gene have been identified in familial and sporadic ALS patients. Previous work from our group identified human ANG (huANG) to protect motoneurons in vitro, and provided proof-of-concept that daily intraperitoneal (i.p.) huANG injections post-symptom onset increased lifespan and delayed disease prog...
Article
A catalase-based microelectrochemical biosensor developed for real-time neurochemical monitoring of hydrogen peroxide (H2O2) was characterised in freely-moving rats. The in situ sensitivity of the sensor was assessed by the direct delivery of H2O2 to the local environment of the implanted sensor and by the chemical manipulation of the endogenous co...
Presentation
Full-text available
Invited speaker presentation (DCU)
Article
Oxygen is of critical importance to tissue viability and there is increasing demand for its reliable real-time clinical monitoring in order to prevent, diagnose and treat several pathological disorders, including hypoxia, stroke and reperfusion injury. Herein we report the development and characterisation of a prototype clinical O2 sensor, and its...
Article
Full-text available
Nicotine dependence is thought to arise in part because nicotine permeates into the endoplasmic reticulum (ER), where it binds to nicotinic receptors (nAChRs) and begins an “inside-out” pathway that leads to up-regulation of nAChRs on the plasma membrane. However, the dynamics of nicotine entry into the ER are unquantified. Here, we develop a famil...
Article
Physiological performance factors of a catalase-based paired microelectrochemical biosensor, developed for real-time neurochemical monitoring of hydrogen peroxide (H2O2), were determined in the in vitro environment. The excellent ascorbic acid (AA) rejection characteristics and high sensitivity of the paired H2O2 sensor were assessed and verified....
Article
Full-text available
Background: Mathematical models of the interactions between alphasynuclein (αS) and reactive oxygen species (ROS) predict a systematic and irreversible switching to damagingly high levels of ROS after sufficient exposure to risk factors associated with Parkinson's disease (PD). Objectives: We tested this prediction by continuously monitoring real-t...
Article
A reliable method of directly measuring endogenously generated nitric oxide (NO) in real-time and in various brain regions is presented. An extensive characterisation of a previously described amperometric sensor has been carried out in the prefrontal cortex and nucleus accumbens of freely moving rats. Systemic administration of saline caused a tra...
Article
Full-text available
A Nafion®-modified Pt sensor developed for real-time neurochemical monitoring in freely-moving animals has now been characterised in vivo for the detection ofnitric oxide (NO). Experiments were undertaken to test sensitivity, selectivity and stability. In control experiments, local administration of aCSF resulted in a decrease in signal (42 ± 12 pA...

Questions

Questions (7)
Question
How long can AAV 2 or 9 be stored at -80 C and used for intracranial injections before it needs re-titre?. Meaning...how fast will it degrade or become less viable.
Question
Numerous dyes available. Need a recommendation for a stable (~1-2 hrs) sensitive (sub-micromolar) dye in-vitro. Looking to measure enzymatic production of hydrogen peroxide from an oxidase enzyme, due to substrate interaction.
Question
Does drug glucuronidation over a number of days change in response to continual administration at the same dose and frequency?.
Looking for any literature evidence supportive or to the contrary.
Question
Is it possible to measure how much of a drug has been converted to drug-gluconoride by the liver. I do not have drug levels in the blood or metabolite in urine.
Question
What is the total volume of blood in the mouse brain?
Question
What is the simplest, benchtop way, to find out the proportion of a drug bound to protein in the brain following systemic treatment in vivo?
Question
Detection of Nicotine from a user over time either in the exhaled breath or through the skin (e.g. transdermal patch) would be ideal. Would appreciate links to publications with exact concentration values please.

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