Sahar Salehi-MüllerUniversity of Bayreuth · Chair of Biomaterials
Sahar Salehi-Müller
PhD.
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62
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Introduction
Additional affiliations
September 2014 - March 2017
September 2013 - August 2014
October 2011 - August 2013
Publications
Publications (62)
Chronic wounds are one of the health challenges threatening human life. In these wounds, overexpression of some types of cytoskeletal actin‐remodeling proteins including Flightless I (Flii) can often lead to severe skin scarring. Herein, arginine functionalized poly(β‐amino ester)s are synthesized to develop polyplexes with alginate for delivery of...
Bioprinting provides a powerful tool for regenerative medicine, as it allows tissue construction with a patient's specific geometry. However, tissue culture and maturation, commonly supported by dynamic bioreactors, are needed. We designed a workflow that creates an implant-specific biore-actor system, which is easily producible and customizable an...
Biomaterials with characteristics similar to extracellular matrix and with suitable bioprinting properties are essential for vascular tissue engineering. In search for suitable biomaterials, this study investigated the three hydrogels alginate/hyaluronic acid/gelatin (Alg/HA/Gel), pre-crosslinked alginate di-aldehyde with gelatin (ADA-GEL), and gel...
Here we develop and characterize a dual-cross-linked pH-responsive hydrogel based on the carboxyethyl chitosan-oxidized sodium alginate (CAO) containing silver nanoparticles (Ag NPs) functionalized with tannic acid/red cabbage (ATR). This hybrid hydrogel is formed via covalent and non-covalent cross-linking. The adhesive strength measured in contac...
Fibrous structures with anisotropic fillers as composites have found increasing interest in the field of biofabrication since they can mimic the extracellular matrix of anisotropic tissues such as skeletal muscle or nerve tissue. In the present work, the inclusion of anisotropic fillers in hydrogel-based filaments with an interpenetrating polymeric...
Rod-like fillers in a flow field of a viscous fluid may form complex structures after passing a sudden contraction. The rods start with a dilute distribution with random positions and orientations. Behind the contraction, a large amount of rods tumble in a spatially correlated way, such that orientations perpendicular to the flow field occur at reg...
Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular pr...
One of the main components of the extracellular matrix (ECM) in natural tissues is collagen. Therefore, there has been a strong focus on processing of collagen for biomaterials application in tissue engineering such as in anisotropic tissues like muscles and tendons. To achieve native‐like mechanical properties of the in vitro processed collagen, v...
The arteriovenous (AV) loop model is a key technique to solve one of the major problems of tissue engineering—providing adequate vascular support for a tissue construct of significant size. However, the molecular and cellular mechanisms of vascularization and factors influencing the generation of new tissue in the AV loop are still poorly understoo...
Development of fiber-spinning technologies and materials with proper mechanical properties is highly important for manufacturing of aligned fibrous scaffolds mimicking structure of the muscle tissues. Here, we report touch spinning of a thermoplastic poly(1,4-butylene adipate)-based polyurethane elastomer, obtained via solvent-free polymerization....
In contrast to conventional hard actuators, soft actuators offer many vivid advantages, such as improved flexibility, adaptability, and reconfigurability, which are intrinsic to living systems. These properties make them particularly promising for different applications, including soft electronics, surgery, drug delivery, artificial organs, or pros...
In situ tissue engineering is an emerging field aiming at the generation of ready-to-use three-dimensional tissues. One solution to supply a proper vascularization of larger tissues to provide oxygen and nutrients is the arteriovenous loop (AVL) model. However, for this model, suitable scaffold materials are needed that are biocompatible/ non-immun...
In bone tissue engineering, the design of in vitro models able to recreate both the chemical composition, the structural architecture, and the overall mechanical environment of the native tissue is still often neglected. In this study, we apply a bioreactor system where human bone-marrow hMSCs are seeded in human femoral head-derived decellularized...
Tissue engineering in reconstructive surgery seeks to generate bioartificial tissue substitutes. The Arterio-Venous (AV)-loop allows the generation of axially vascularized tissue constructs. Cellular mechanisms of this vascularization process are largely unclear. In this study, we developed two different chamber models for intravital microscopy and...
Poly(glycerol sebacate) (PGS) continues to attract attention for biomedical applications owing to its favorable combination of properties. Conventionally polymerized by a two‐step polycondensation of glycerol and sebacic acid, variations of synthesis parameters, reactant concentrations or by specific chemical modifications, PGS materials can be obt...
A comprehensive investigation of four polydiketopyrrolopyrroles (PDPPs) with increasing ethylene glycol (EG) content and varying nature of comonomer is presented, and guidelines for the design of efficient mixed ion‐electron conductors (MIECs) are deduced. The studies in NaCl electrolyte‐gated organic electrochemical transistors (OECTs) reveal that...
This paper reports an approach for the fabrication of shape-changing bilayered scaffolds, which allow the growth of aligned skeletal muscle cells, using a combination of 3D printing of hyaluronic acid hydrogel, melt electrowriting of thermoplastic polycaprolactone-polyurethane elastomer, and shape transformation. The combination of the selected mat...
We report the fabrication of scroll-like scaffolds with anisotropic topography using 4D printing based on a combination of 3D extrusion printing of methacrylated alginate, melt-electrowriting of polycaprolactone fibers, and shape-morphing of the fabricated object. A combination of 3D extrusion printing and melt-electrowriting allows programmed depo...
Developing a transparent substitute with high water permeability and similar mechanical properties to cornea is one of the major challenge in corneal tissue engineering. Here, transparent hybrid films based on silk nanofibrils (SNF)/gelatin methacryloyl (GelMA) are introduced for cornea tissue engineering. The mechanical properties, transparency, d...
3D printing is a rapidly evolving field for biological (bioprinting) and non-biological applications. Due to a high degree of freedom for geometrical parameters in 3D printing, prototype printing of bioreactors is a promising approach in the field of Tissue Engineering. The variety of printers, materials, printing parameters and device settings is...
3D printing is a rapidly evolving field for biological (bioprinting) and non-biological applications. Due to a high degree of freedom for geometrical parameters in 3D printing, prototype printing of bioreactors is a promising approach in the field of Tissue Engineering. The variety of printers, materials, printing parameters and device settings is...
Engineering functional skeletal muscle tissue is an ongoing challenge because of the complexity of the in vivo microenvironment and the various factors that contribute to the development and maintenance of the native tissue. However, the growing understanding of the natural skeletal muscle's microenvironment in vivo, as well as the ability to succe...
In this paper, we describe the application of the 4D biofabrication approach for the fabrication of artificial nerve graft. Bilayer scaffolds consisting of uniaxially aligned polycaprolactone-poly(glycerol sebacate) (PCL-PGS) and randomly aligned methacrylated hyaluronic acid (HA-MA) fibers were fabricated using electrospinning and further used for...
Due to its properties, such as biodegradability, low density, excellent biocompatibility and unique mechanics, spider silk has been used as a natural biomaterial for a myriad of applications. First clinical applications of spider silk as suture material go back to the 18th century. Nowadays, since natural production using spiders is limited due to...
Reconstructive surgery remains inadequate for the treatment of volumetric muscle loss (VML). The geometry of skeletal muscle defects in VML varies on a case-by-case basis. Three-dimensional (3D) printing has emerged as one strategy that enables the fabrication of scaffolds that match the geometry of the defect site. However, the time and facilities...
The current gold standard in peripheral nerve repair is nerve autografts for bridging gaps larger than a centimeter. However, autografts are associated with a low availability and the loss of function at the donor site. Nerve guidance conduits (NGCs) made of biocompatible and biodegradable materials reflect suitable alternatives. Clinically approve...
Skeletal muscle is one of the most abundant tissues in the body. Although it has a relatively good regeneration capacity, it cannot heal in the case of disease or severe damage. Many current tissue engineering strategies fall short due to the complex structure of skeletal muscle. Biofabrication techniques have emerged as a popular set of methods fo...
Hemostatic adhesive hydrogels as sealants for surgical operations are one of the focus of the researches in the field of injectable materials. Herein, we evaluated the potential application of a mechanically robust nanocomposite hydrogel with significant adhesion strength and shorter blood clotting time. This hydrogel was composed of thiolated gela...
Native tissues orchestrate their functions by complex interdependent cascades of biochemical and biophysical cues that vary spatially and temporally during cellular processes. Scaffolds with well-tuned structural, mechanical, and biochemical properties have been developed to guide cell behavior and provide insight on cell-matrix interaction. Howeve...
Skeletal muscle tissue engineering (SMTE) aims at repairing defective skeletal muscles. Until now, numerous developments are made in SMTE; however, it is still challenging to recapitulate the complexity of muscles with current methods of fabrication. Here, after a brief description of the anatomy of skeletal muscle and a short state‐of‐the‐art on d...
Various tissue-engineering approaches have been considered for fabricating viable cornea-shaped tissue equivalents for lamellar and full-thickness corneal transplants. These equivalents must simulate the optical and mechanical properties of the native cornea. Engineering of different layers of the cornea, such as the epithelium, stroma, and endothe...
Bioengineered functional muscle tissues are beneficial for regenerative medicine due to their treatment potential for various debilitating disorders, including myopathy and traumatic injuries. However, the contractile properties of engineered muscle constructs are lacking compared to their native counterparts. Here, we used microfluidic spinning to...
Composite coatings of Hydroxyapatite (HA) with ceramics, polymers and metals are used to modify the surface structure of implants. In this research, HA/TiO2 composite coating was fabricated by electrophoretic deposition (EPD) on 316 stainless steel substrate. HA/TiO2 composite coatings with 5, 10 and 20 wt.% of TiO2, deposited at 40 V and 90 s as a...
Silk is a well-known and unique material that has been used in different applications for more than 5,000 years. The shiny silk fabrics that we know are actually made of threads created by the Chinese silkworm, but various arthropods can produce silks. Spider silk, for instance, is known to be the toughest fibre on Earth, outperforming both natural...
Tissue engineering (TE) and regenerative medicine, with the aim to replace, repair and remodel the damaged tissues and organs, has led to development of myriad biomimetic materials that possess biomechanical properties resembling those of native tissues. Some of the key points to generate a successful replacement for native tissues are selecting th...
Skeletal muscle tissues engineered in vitro are aneural, short in number of fibers required to function properly, and degenerate rapidly. Electrical stimulation has been widely used to compensate for such lack of neural activity, yet the relationship between the stimulation parameters and the tissue response is subject of debate. Here we study the...
Cell transplantation therapy provides a potential solution for treating skeletal muscle disorders, but cell survival after transplantation is poor. This limitation could be addressed by grafting donor cells onto biomaterials to protect them against harsh environments and processing, consequently improving cell viability in situ. Thus, we present he...
Statement of significance:
In corneal tissue engineering a major challenge is the development of synthetic scaffolds with similar properties to native cornea. In our recent works, we introduced the biodegradable, polymeric nanofibrous scaffolds with similar optical and mechanical properties for corneal regeneration and here we examined the cyto- a...
We employed the large surface-to-volume ratio of nanoporous gold (NPG) to build a set of highly sensitive superoxide electrochemical biosensors. Once functionalized with cytochrome-c (cyt-c) the NPG-based electrodes displayed a superoxide sensitivity of 0.24 nA nM −1 and a low-level of detection of 5 nM towards superoxide. The planar electrodes wer...
Online detection and accurate quantification of superoxide anions released from skeletal muscle tissue is important both in physiological and pathological contexts. Above certain physiologically redundant levels, superoxides may exert toxic effects. Here we present design, fabrication, and successful testing of a highly sensitive electrochemical su...
Real-time monitoring of metabolically relevant biochemicals released in minuscule amounts is of utmost diagnostic importance. Superoxide anion as a primary member of reactive oxygen species, has physiological and pathological effects that depend on its concentration and release rate. Here we present fabrication and successfully testing of a highly...
Skeletal muscle tissue engineering is one of the important ways for regenerating functionally defective muscles. Among the myopathies, the Duchenne muscular dystrophy (DMD) is a progressive disease due to mutations of the dystrophin gene leading to progressive myofiber degeneration with severe symptoms. Although current therapies in muscular dystro...
Hydrogels are hydrophilic polymer networks with high water content, which have played an important role as scaffolds for cells, as carriers for various biomolecules (e.g. drugs, genes, and soluble factors), and as injectable biomaterials in tissue engineering (TE) and regenerative medicine. Bioconjugation is an approach for improving the performanc...
Superoxide anion (SOA) as a member of reactive oxygen species (ROS) group is involved in various physiological and pathological states. For instance, generation of SOA is known to increase with skeletal muscle contractile activity and fatigue. It is therefore important to selectively detect and accurately quantify the release of SOA within both phy...
Highly ordered block copolymer thin films have been studied extensively during the last years because they afford versatile self assembled morphologies via a bottom-up approach. They promise to be used in applications such as polymeric membranes or templates for nanostructured materials. Among the block copolymer structures, perpendicular cylinders...
Biodegradable elastomeric materials such as poly glycerol sebacate (PGS) have gained much current attention in the field of soft tissue engineering. The present study reports the synthesis of PGS with molar ratios of 1:1, 2:3, and 3:2 of glycerol and sebacic acid via polycondensation reaction and tests the effect of PGS on human corneal epithelial...
Background:
Amniotic membranes have been used for many years for reconstruction of the ocular surface. Despite having anti-inflammatory and antiangiogenic properties as well as being suitable as a carrier for corneal epithelial cells, amniotic membranes also have some limitations for use at the human cornea: availability is limited, there are majo...
Hydroxyapatite/alumina nanocrystalline composite powders needed for various biomedical applications were successfully synthesized by sol–gel process. Structural and morphological investigations of the prepared composite powders were performed using X-ray diffractometer (XRD), scanning electron microscopy (SEM), X'Pert HighScore software, and Clemex...
Generation of a tissue-engineered cornea composed of biomaterials and cells will reduce dependency on the short supply of donor corneal tissue. In the last decade, the supply of viable donor tissue for corneal transplants is relatively less than the demand for donor corneal tissue. A tissue- engineered cornea that incorporates cells with a biocompa...
Background:
An estimated 10 million people suffer worldwide from vision loss caused by corneal damage. For the worst cases, the only available treatment is transplantation with human donor corneal tissue. However, in numerous countries there is a considerable shortage of corneal tissue of good quality, leading to various efforts to develop tissue...
Physical and mechanical studies of aligned nanofibers of poly (glycerol sebacate) (PGS)/ poly (ε-caprolactone) (PCL) designed for application as cornea tissue engineering are investigated. The fibers were fabricated by electrospinning at different weight ratios of PGS and PCL (1:1, 2:1, 3:1, and 4:1) and had diameters in the range of 300 –550 nm. D...
Morphological studies of aligned nanofibers scaffolds made of poly(glycerol sebacate)(PGS)/poly(ε-caprolactone)(PCL) blends for application as corneal tissue scaffolds. Parallel conductive bars as ground electrode have been used to generate unidirectional nanofibers. Scaffolds have fibers diameter in the range of 550–300 nm. The structural stabilit...
This contribution will report on nanofibrous scaffolds for corneal tissue engineering. By electrospinning, biodegradable nanofibers can be created that form a semitransparent layer. This can be used as a substrate for further cell seeding procedures. It is the goal to use this scaffold in ocular surface reconstruction.
In this study, nanostructured composite coatings of hydroxyapatite (HA)/ 30wt% yttria stabilized zirconia (YSZ) coatings containing 0, 3, 5, and 8 mol% Y2O3 (namely; HA-0YSZ, HA-3YSZ, HA-5YSZ, and HA-8YSZ) were successfully synthesized using the sol-gel method. The crystallite size of the coating was about ~44-58 nm for tetragonal and cubic zirconi...
In this study, nanostructured composite coatings of hydroxyapatite (HA)/ 30wt% yttria stabilized zirconia (YSZ) coatings containing 0, 3, 5, and 8 mol% Y2O3 were successfully synthesized using the sol-gel method. The crystallite size of the coating was about ~44-58 nm for tetragonal and cubic zirconia grain size and 75-87 nm for hydroxyapatite grai...
Homogeneous composite nanopowders of hydroxyapatite/30wt% yttria-stabilized zirconia (HA–YSZ) containing 0, 3, 5, and 8mol% Y2O3 (namely; HA–0YSZ, HA–3YSZ, HA–5YSZ, and HA–8YSZ) were successfully synthesized using the sol–gel method. Simultaneous thermal analysis (STA), X-ray diffraction (XRD), X-ray fluorescence spectroscopy (XRF), Fourier transfo...