Saghar Yousefnia

Saghar Yousefnia
University of Isfahan · Department of Biology

PhD of cellular and molecular biology

About

13
Publications
14,355
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201
Citations
Introduction
Saghar Yousefnia recently got PhD degree from Department of Biology, University of Isfahan. Saghar does research in Molecular Biology, Cancer Research and Cell Biology.
Additional affiliations
February 2015 - January 2020
Royan Institute, Isfahan, Iran
Position
  • PhD Student

Publications

Publications (13)
Article
Full-text available
Acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia (AML) characterized with a translocation between promyelocytic leukemia gene (PML) on chromosome 15 and retinoic acid receptor alpha gene (RARα) on chromosome 17. Transcription of this fusion gene results in PML/RARα fusion protein blocking expression of critical genes involved...
Article
Full-text available
Breast cancer stem cells (BCSCs) are the minor population of breast cancer (BC) cells that exhibit several phenotypes such as migration, invasion, self-renewal, and chemotherapy as well as radiotherapy resistance. Recently, BCSCs have been more considerable due to their capacity for recurrence of tumors after treatment. Recognition of signaling pat...
Article
Full-text available
Background Mammospheres are breast cancer stem cells (BCSCs) that could be yielded through culturing cells in non-adherent and non-differentiating condition. With regard to therapy resistance of cancer stem cells (CSCs), it is essential to discover efficient approaches targeting CSCs. Viola odorata extract has been considered as a traditional herba...
Article
Full-text available
Keywords: Macular Degeneration Disease; NQO1; Polymorphism; Age at Onset Background: Oxidative stress is one of the most important reasons of age-related macular degeneration disease (AMD) that is caused by smoking, U.V. and X ray exposure, pressure and heat which result in increasing of reactive oxygen species (ROS) level. As
Poster
Full-text available
Article
Full-text available
Stemness phenotype mammospheres established from cell lines and tissues taken from autopsy can be used to test and to identify the most sensitive drugs for chemotherapy. Therefore, the aim of the present study was isolation and characterization of cancer stem cells derived from MCF7, MDA-MB231, and SKBR3 breast cancer cell lines to demonstrate the...
Article
One of the most importantly involved pathways in cancer development is fatty-acid signaling pathway. Synthesized lipids as energetic sources are consumed by cancer cells for proliferation, growth, survival, invasion and angiogenesis. Fatty acids as signaling compounds regulate metabolic and transcriptional networks, survival pathways and inflammato...
Article
Peroxisome proliferator-activated receptor γ (PPARγ) belongs to the nuclear receptor superfamily of PPARs (PPARα, PPARβ/δ, PPARγ). Numerous studies have concentrated on the key role of PPARs in inflammation and a variety of cancers which include prostate, breast, glioblastoma, neuroblastoma, pancreatic, hepatic, leukemia, and bladder and thyroid tu...
Article
Full-text available
Peroxisome proliferator-activated receptor γ (PPARγ) belongs to the nuclear receptor superfamily of PPARs (PPARα, PPARβ/δ, PPARγ). Numerous studies have concentrated on the key role of PPARs in inflammation and a variety of cancers which include prostate, breast, glioblastoma, neuroblastoma, pancreatic, hepatic, leukemia, and bladder and thyroid tu...
Article
Full-text available
Background: Cells have complex network of antioxidant enzymes that protect cells from induced damages by reactive oxygen species (ROS). Catalase and superoxide dismutase are known for their role as primary protection against oxidative stress. Oxidative damage is an important risk factor in age-related macular degeneration disease (AMD). For the fir...

Questions

Questions (9)
Question
I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show cancer stem cell properties.I follow this protocol for isolating:
1-Seeding 20,000 cells/mL in free-serum DMEM/F12 in agar coated flask
2-Adding 20 ng/ml bFGF and 20 ng/ml EGF, 2%B7.
3-Adding bFGF and EGF every two days.
4-Harvesting cells after 7 days.
May you help me why i dont see CD44+ spheres? I am waiting for your response. I hope your helpful response makes me close to my purpose.
Question
Hi, has anyone experience about mammosphere formation in 3004 greiner dishes?
Question
Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?
Question
Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines? does it Interfere with the mammosphere formation?
Question
Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?
Question
which kind of plates and sizes (6, 24, 96 well) are suitable for mammosphere formation?
Question
Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?can anyone tell me about advantages and disadvantages?
Question
i want to isolate breast cancer stem cells from breast cancer cell line with purpose of drug researches. can anyone suggest me which cell line is better to isolate?  is it possible for any kinds of breast cancer cell line?

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Projects

Projects (5)
Project
Understanding the critical role of miR-153 in cell growth, metastasis, angiogenesis and drug resistance ability of cancer cells, suggests miR-153 as a potential prognostic biomarker for detecting cancer as well as providing a novel treatment strategy to combat with several types of cancer.
Project
Recognition of signaling pathways and molecular mechanisms induced by ATO can be effective for discovering novel treatment strategies to target leukemia cells. Also, it can be developed for treatment of variety of cancer cells.