Saghar YousefniaSemnan University · Department of Cell and Molecular Biology
Saghar Yousefnia
PhD of cellular and molecular biology
Assistant professor at Semnan University
About
24
Publications
19,984
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Introduction
I got PhD degree from Department of Biology, University of Isfahan. My research fields are Molecular Biology, Cancer Research and Cell Biology. Currently, I work at Semnan University as an assistant professor.
Additional affiliations
February 2015 - January 2020
Royan Institute, Isfahan, Iran
Position
- PhD Student
Publications
Publications (24)
miRNAs are well-known as non-coding RNAs which regulate genes related to cancer progression. They can regulate their target genes, negatively and play a critical role as oncogenic and tumor-suppressive miRNAs. They can inhibit and promote cancer development through suppressing the expression of oncogenic and tumor-suppressive genes, respectively. T...
One of the most dangerous cancers is the malignancy related to blood cells such as variety types of leukemias, lymphomas and multiple myeloma. Early diagnosis of cancers can be very effective in increasing the chances of survival. In this review, the use of aptamers as novel molecular diagnostic tools against leukemia, multiple myeloma, and lymphom...
Long non-coding RNAs (lncRNAs) are versatile RNAs that regulate various cellular processes, such as gene regulation, by acting as signals, decoys, guides, and scaffolds. A novel recognized lncRNA, LOXL1-antisense RNA 1 (LOXL1-AS1), is dysregulated in some diseases, including cancer, and acts as an oncogenic lncRNA in many types of cancer cells. Upr...
Metallothionein 1A (MT1a) is involved in many pathological conditions associated with antioxidant defense and detoxification, including cancer. The aim of this study was to investigate the possible association of MT1A rs11640851, rs8052394, and rs11076161 single nucleotide polymorphisms (SNPs) with the risk of breast cancer (BC) and clinicopatholog...
Mesoporous silica nanoparticles (MSNs) have attracted sufficient attention as one of the most capable carriers for the delivery of various cancer therapy and imaging agents. In the current study, MSNs with some modifications such as covering their surface with an anti-PSMA-aptamer functionalized thermo-responsive cationic-lipid coverage were used t...
Background:
A growing body of evidence indicates that oxidative stress, high levels of reactive oxygen species (ROS), is implicated in the pathogenesis of breast cancer (BC). Superoxide dismutase (SOD2), a mitochondria-resident antioxidant enzyme, protects cells from ROS by catalytically converting the superoxide radicals into less reactive specie...
Herbal medicines have various active ingredients with special therapeutic properties. In this study, lemongrass essential oil (LEO) was transferred to biocompatible nanoliposomes as a cytotoxic agent against breast cancer cells. The constituents of LEO were measured by gas chromatography coupled with mass spectrometry (GC-MS). The physicochemical p...
Severe acute respiratory syndrome (SARS) coronavirus ۲ (SARS-CoV۲) leading to COVID-۱۹ has initiated a catastrophe for humans since December ۲۰۱۹. Genetic and protein similarities between SARS-CoV and SARS-CoV۲ offer the same treatments for both types of virus. However, there are some sequence or structural differences between SARS-CoV۲ and SARS-Co...
miRNAs play a crucial role in regulating genes involved in cancer progression. Recently, miR-153 has been mainly well-known as a tumor suppressive miRNA modulating genes in proliferation, metastasis, EMT, angiogenesis and drug resistance ability of a variety types of cancer. Mechanistic activity of miR-153 in tumorigenicity has not been fully revie...
Acute promyelocytic leukemia (APL), a subtype of acute myeloid leukemia (AML) characterized with a translocation between promyelocytic leukemia gene (PML) on chromosome 15 and retinoic acid receptor alpha gene (RARα) on chromosome 17. Transcription of this fusion gene results in PML/RARα fusion protein blocking expression of critical genes involved...
Breast cancer stem cells (BCSCs) are the minor population of breast cancer (BC) cells that exhibit several phenotypes such as migration, invasion, self-renewal, and chemotherapy as well as radiotherapy resistance. Recently, BCSCs have been more considerable due to their capacity for recurrence of tumors after treatment. Recognition of signaling pat...
Background
Mammospheres are breast cancer stem cells (BCSCs) that could be yielded through culturing cells in non-adherent and non-differentiating condition. With regard to therapy resistance of cancer stem cells (CSCs), it is essential to discover efficient approaches targeting CSCs. Viola odorata extract has been considered as a traditional herba...
Keywords: Macular Degeneration Disease; NQO1; Polymorphism; Age at Onset Background: Oxidative stress is one of the most important reasons of age-related macular degeneration disease (AMD) that is caused by smoking, U.V. and X ray exposure, pressure and heat which result in increasing of reactive oxygen species (ROS) level. As
Stemness phenotype mammospheres established from cell lines and tissues taken from autopsy can be used to test and to identify the most sensitive drugs for chemotherapy. Therefore, the aim of the present study was isolation and characterization of cancer stem cells derived from MCF7, MDA-MB231, and SKBR3 breast cancer cell lines to demonstrate the...
One of the most importantly involved pathways in cancer development is fatty-acid signaling pathway. Synthesized lipids as energetic sources are consumed by cancer cells for proliferation, growth, survival, invasion and angiogenesis. Fatty acids as signaling compounds regulate metabolic and transcriptional networks, survival pathways and inflammato...
Peroxisome proliferator-activated receptor γ (PPARγ) belongs to the nuclear receptor superfamily of PPARs (PPARα, PPARβ/δ, PPARγ). Numerous studies have concentrated on the key role of PPARs in inflammation and a variety of cancers which include prostate, breast, glioblastoma, neuroblastoma, pancreatic, hepatic, leukemia, and bladder and thyroid tu...
Peroxisome proliferator-activated receptor γ (PPARγ) belongs to the nuclear receptor superfamily of PPARs
(PPARα, PPARβ/δ, PPARγ). Numerous studies have concentrated on the key role of PPARs in inflammation and
a variety of cancers which include prostate, breast, glioblastoma, neuroblastoma, pancreatic, hepatic, leukemia,
and bladder and thyroid tu...
Background: Cells have complex network of antioxidant enzymes that protect cells from induced damages by reactive oxygen species (ROS). Catalase and superoxide dismutase are known for their role as primary protection against oxidative stress. Oxidative damage is an important risk factor in age-related macular degeneration disease (AMD). For the fir...
Questions
Questions (10)
For my project, I use MG63 cell line. These cells grew well in the first passage. But after the second passage, there were many dead cells and the culture medium became turbid and powdery (The culture medium placed in the incubator alone, its turbidity did not increase). Also, the culture medium was cultured on agar to determine bacterial contamination but did not show any contamination. In the next days, the attached cells also detached and died. Also, after changing the culture medium, the orange color of the medium quickly changes to pink within an hour (fresh complete medium contains 10% serum, 1% NEAA, 1% L-glu and 1% pen/strp). What is the cause of this cell death and change in color and turbidity of the culture media? Thankful
I am trying to isolate breast cancer stem cells from breast cancer cell lines through mammosphere formation. I see perfect spheres under microscope but all of them are CD24+/CD44- and dont show cancer stem cell properties.I follow this protocol for isolating:
1-Seeding 20,000 cells/mL in free-serum DMEM/F12 in agar coated flask
2-Adding 20 ng/ml bFGF and 20 ng/ml EGF, 2%B7.
3-Adding bFGF and EGF every two days.
4-Harvesting cells after 7 days.
May you help me why i dont see CD44+ spheres? I am waiting for your response. I hope your helpful response makes me close to my purpose.
Hi, has anyone experience about mammosphere formation in 3004 greiner dishes?
Hi, i want to use 20ng/ml BFGF in culture media. How can i dilute 35 microgram bFGF/35 microliter Tris 5mM without any problem for breast cancer stem cells?
Is it necessary to use β-Mercaptoethanol in MCF-7 culture media or other breast cancer cell lines? does it Interfere with the mammosphere formation?
Is it possible to use low attachment cell plates instead of ultra-low attachment plates for mammosphere formation with a same efficiency?
which kind of plates and sizes (6, 24, 96 well) are suitable for mammosphere formation?
Is there any difference in quality between isolation of cancer stem cells from tissues and cell line?can anyone tell me about advantages and disadvantages?
i want to isolate breast cancer stem cells from breast cancer cell line with purpose of drug researches. can anyone suggest me which cell line is better to isolate? is it possible for any kinds of breast cancer cell line?
