Sagar Regmi

Case Western Reserve University School of Medicine · Department of Pharmacology

Circulating tumor cells (CTCs) are the primary targets of cancer treatment as they cause distal metastasis. However, how CTCs response to exercise-induced high shear stress is largely unknown. To study the effects of hemodynamic microenvironment on CTCs, we designed a microfluidic circulatory system that produces exercise relevant shear stresses. W...

Purpose Many anti-cancer drugs are used in chemotherapy; however, little is known about their efficacy against circulating tumor cells (CTCs). In this study, we investigated whether the pulsatile fluidic shear stress (SS) in human arteries can affect the efficacy of anti-cancer drugs. Methods Cancer cells were circulated in our microfluidic circul...

15-prostaglandin dehydrogenase (15-PGDH) is a negative regulator of tissue stem cells that acts via enzymatic activity of oxidizing and degrading PGE2, and related eicosanoids, that support stem cells during tissue repair. Indeed, inhibiting 15-PGDH markedly accelerates tissue repair in multiple organs. Here we have used cryo-electron microscopy to...

The application of cold plasma therapy for the treatment of cancer is one promising field of study. This therapy can target and kill cancer cells while sparing healthy cells. The reactive oxygen species (ROS) and reactive nitrogen species (RNS), produced during plasma treatment is responsible for the cancer cells death. The research on cold plasma...

Sialic acid (SA) is a well-known component of glycoproteins, which have applications in various functional processes on the cell's surface. The colorimetric is a simpler and more convenient method for measuring SA due to its low-cost apparatus and visual signal changes. This work focused on the unpredictable interparticle crosslinking aggregation o...

Understanding the dynamic changes in antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for evaluating the effectiveness of the vaccine and the stage for the recovery of the COVID-19 disease. A rapid and accurate method for the detection of SARS-CoV-2-specific antibodies is still urgently needed. Here, we d...

The unbalanced lifestyle and rampant use of modern medicine are the leading causes of life-threatening cancer disease prevalence. In the last few decades, chemotherapy and other medication techniques promised to cure cancer. However, cold plasma on cancer cell lines gets little attention due to a lack of understanding of the detailed mechanism of a...

Significant advanced have recently been made in exploiting microneedle-based (MN-based) diabetes devices for minimally invasive wearable biosensors and for continuous glucose monitoring. Within this emerging class of skin-worn MN-based sensors, the ISF can be utilized as a rich biomarker source to diagnose diabetes. While initial work of MN devices...

Significant advanced have recently been made in exploiting microneedle-based (MN-based) diabetes devices for minimally invasive wearable biosensors and for continuous glucose monitoring. Within this emerging class of skin-worn MN-based sensors, the ISF can be utilized as a rich biomarker source to diagnose diabetes. While initial work of MN devices...

Having its root lying in microelectronics, microfluidics seems to hold great potential to explore people's limited knowledge in the field of oncology. It offers numerous advantages such as a low sample volume, minimal cost, parallelization, and portability and has been advanced in the field of molecular biology and chemical synthesis. The platform...

Taking the life of nearly 10 million people annually, cancer has become one of the major causes of mortality worldwide and a hot topic for researchers to find innovative approaches to demystify the disease and drug development. Having its root lying in microelectronics, microfluidics seems to hold great potential to explore our limited knowledge in...

A novel natural hydroxyapatite (HAp) bioceramic was extracted from the ostrich cortical bone by the thermal decomposition method. HAp was characterized by different analytical tools such as thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, and scanning electron microscopy (SEM). Remo...

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and first detected in Wuhan, Hubei Province, China in December 2019 and spread across China and abroad. On 11th March 2020, WHO officially declared pandemic on for COVID-19. This review presents transmission mode of the COVID-19 in terms of...

Abstract Purpose Many anti-cancer drugs are used in chemotherapy; however, little is known about their efficacy against circulating tumor cells (CTCs). In this study, we investigated whether the pulsatile fluidic shear stress (SS) in human arteries can affect the efficacy of anti-cancer drugs. Methods Cancer cells were circulated in our microflui...

Graphene quantum dots (GQD), the new zero‐dimensional carbon nanomaterial, has been demonstrated as a promising material for biomedical applications due to its good biocompatibility and low toxicity. However, the integration of multiple therapeutic approaches into a nano‐sized platform based on the GQD has not been explored yet to our best knowledg...

Cancer metastasis is a complicated disease that causes around 90% of cancer deaths. It is one of the deadly diseases around the world killing almost 8.8 million people in 2015. Exercise, anti-cancer drugs, and anti-oxidants have attracted the interest in the rehabilitation of the cancer patients. Insights on the mechanism of prevention facilitate t...

PhD Dissertation Defense Slides

Circulating tumor cells (CTCs) are mainly responsible for the cause of cancer metastasis. Although most CTCs can be destroyed by the bloodstream, some of them can still manage to withstand hemodynamic shear stress in blood stream. To study the effects of fluidic micro-environment on CTCs, we designed a microfluidic system that can produce various l...

The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection. The guanine-rich U3 region within the LTR promoter, previously shown to form G-quadruplex structures, represents an attractive target to inhibit HIV transcription and replication. In this work, we re...

Background: Deaths due to cardiovascular diseases (CVD) are increasing day by day. Although there have been studies on risk factors for CVD amongst different population, no study has been done to assess prevalence of risk factors for CVD on employees of academic institutions. Materials and Methods: A cross-sectional study was conducted to determine...

ABSTRACT Background: Tuberculosis (TB) is among the top 10 causes of death in children. Childhood TB remains a major health burden because of diagnostic and treatment delays. Objective: To determine time to diagnosis and factors associated with it in children less than 15 years of age registered as new cases under RNTCP. Materials and Methods: Cros...