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I am Sagar Aryal, a passionate Microbiologist and the Scientific Blogger. I did my Master’s Degree in Microbiology and currently doing my Ph.D. from Tribhuvan University. I am particularly interested in research related to actinomycetes, myxobacteria and natural products.
February 2015 - June 2019
- - Involvement in Theory and Practical Classes - To supervise Master Level Dissertation work - To publish and work for SXC Journal of Science and Technology - To conduct research and project works - Research and Hospital Related Works
Plastic pollution in various forms has emerged as the most severe environmental threat. Small plastic chunks, such as micro-plastics and nanoplastics derived from primary and secondary sources, are a major concern worldwide due to their adverse effects on the environment and public health. Several years have been spent developing robust spectroscop...
Antibiotics, nowadays, are not only used for the treatment of human diseases but also used in animal and poultry farming to increase production. Overuse of antibiotics leads to their circulation in the food chain due to unmanaged discharge. These circulating antibiotics and their residues are a major cause of antimicrobial resistance (AMR), so comp...
Wildlife Diplomacy in the form of exchange, gift, or via loan has been experienced and used all over the world, and its commonly found in the Hindu Kush-Himalaya region also. Nepal, as an independent country, had followed Hunting Diplomacy previously. However, Rhinoceros (Rhino) Diplomacy is specifically famous as a means of increasing foreign rela...
Streptomyces species are very well known for the production of antimicrobials and enzymes. The objective of this review is to search for the novel Streptomyces isolated in 2018 along with their characteristics and possible antimicrobial properties. The literature search was done according to the PRISMA flow diagram where 28 research papers were eli...
Actinomycetes are Gram-positive, facultative anaerobic fungus-like filamentous bacteria which remain on the top of the natural antibiotic producers. Due to the climatic and geographical diversity of Nepal, a wide range of microorganisms with potent source of antimicrobials are available. The objective of this study was to isolate, identify, and scr...
Human Coronaviruses (HCoV) exhibit positive single stranded RNA genome with enveloped nucleocaps id. Coronavirus belong to the family Coronaviridae, originated from avian and mammalian species causes upper respiratory tract infection in humans by novel HCoVs viruses named as HCoV-HKU1, HCoV-NL63 but predominant species is Middle East respiratory sy...
Actinomycetes are considered as the most invaluable prokaryotes whose genome mining show a great number of putative secondary metabolite biosynthesis pathways as well as gene clusters ranging from 20 to 50 per genome. The genus Streptomyces has been explored for its ability to produce 60% antibiotics worldwide. Alnus nepalensis (Alder) has been fou...
p>Oral cavity is the easiest site of entry of microorganisms during breathing, eating, drinking and brushing which can lead to several bacterial infections in oral cavity, pharynx, larynx, gastrointestinal tracts etc. Toothbrushes commonly used to maintain oral health and prevent dental disease; but unfortunately, how keeping the toothbrush is negl...
Background: Bacillus genus have been found to produce antimicrobial polypeptides that have a remarkable capability to combat the current increasing problem of antibiotic resistance. The aim of this cross sectional study was to determine the proportion and pattern of distribution of antibiotic producing and non-producing species of Bacillus in soil...
Water covers seven tenths of the Earth's surface and occupies an estimated total volume of 1,386,000,000 cubic kilometers (km3). Of all the water found on Earth, 97% is marine. Maximum of this water is at a temperature of 2 to 3°C and devoid of light; 62% is under high pressure (>100 atm). Microscopic phytoplankton and associated bacteria generate...
My research topic is "Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis isolated from selected hospitals in Kathmandu Valley".
I need positive control of rpoB, katG, inhA and gyrB genes. So can any one provide me the positive control of these MDR genes ?
I need these positive controls to do PCR and gel electrophoresis.
What are ethnic and indigenous diversities? What are their roles in conservation of nature and natural species? How a good governance can be created/produced with the help of ethnic and indigenous diversities? Plan, Policy, and Programs to upgrade ethnic and indigenous people as well as conserve biodiversity.
Research Objectives 1. Isolation, Screening and Identification of antimicrobial Actinomycetes from varied altitude. 2. Phylogenetic identification of potential strains based on 16s rRNA gene sequencing. 3. To describe the biogeography of Actinomycetes of Nepal through metagenomics. 4. Extraction, purification and antimicrobial potency of bioactive compounds. 5. Identification of target functional genes associated with synthesis of specific bioactive compounds.