Saeid Malekzadeh Shafaroudi

• Ferdowsi University of Mashhad

Background Onion seeds have limited storage capacity compared to other vegetable seeds. It is crucial to identify the mechanisms that induce tolerance to storage conditions and reduce seed deterioration. To address this goal, an experiment was conducted to evaluate changes in germination, biochemical, physiological, and molecular characteristics of...

Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saline water lake. Chlorella vulgaris, Chlorella sorokiniana, Chlamydomonas raudensis, Chlamydomonas hedleyi, Duna...

Algal bioprospecting in ecosystems leads to exploring native microalgae and the competency evaluation of economically producing lipids as biofuel or nutritional applications. In this study, ten microalgae species were screened from the saltwater lake. Chlorella vulgaris , Chlorella sorokiniana , Chlamydomonas raudensis , Chlamydomonas hedleyi , Dun...

The leaf stripe disease of barely caused by the seed transmitted hemi-biotrophic fungal pathogen, Pyrenophora graminea is an important disease of barley worldwide. The present study was carried out to determine the genetics of leaf stripe resistance in an association mapping population consisted of 141 European two-rowed spring barley cultivars and...

ndophytic fungi are often producing host plant metabolites. Tanshinones are secondary metabolites of the Salvia genus which are also produced by some endophytic fungi. Efficient secondary metabolite production in endophytic fungi drops significantly after sequential subcultures. 5-azacytidine (5-AC) is an analog of the naturally occurring pyrimidin...

Background: Datura innoxia Mill. is one of the medicinal plant which can produce tropan alkaloids such as hyoscyamine and scopolamine with analgesic and antiasthmatic activities. Objective: In this research, the effect of various factors such as culture media and bacteria strain were studied on hairy roots induction and secondary metabolites produc...

PMF (Plant Molecular Farming) consists of repurposing intact plants or plant cell cultures as an alternative and novel heterologous expression system to produce recombinant proteins. It is safer and more cost-effective to produce recombinant proteins using plant cells rather than traditional expression platforms like bacteria and mammalian cells. A...

In order to identify different landraces of onion seeds in terms of germination characteristics, an experiment was conducted in a completely randomized design with four replications in the Biology Laboratory of the Faculty of Agriculture, Ferdowsi University of Mashhad in 2020. The experimental treatments were 29 native landraces of onion seeds fro...

Background: Datura species produce a wide range of tropane alkaloids with medicinal values. D. innoxia is a species of the Datura genus which is poisonous and has an unpleasant odor due to the presence of tropane alkaloids. Objective: The study aimed to induction of polyploidy by colchicine treatment on downy thorn-apple and the effect of it on app...

Background: Papaver bracteatum Lindl. (Persian poppy) has a very long history as the major source of natural alkaloids. Among Papaver species, Persian poppy can be considered as a rich source of thebaine. Objective: The purpose of this study was to evaluate the thebaine content in different organs and different populations of Persian poppy. Methods...

مصرف انرژی و تاثیر آن بر محیط‌زیست یکی از بزرگترین چالش‌های بشر است و تولید اتانول زیستی یا سایر مواد شیمیایی به وسیله هیدرولیز آنزیمی سلولز می‌تواند تاثیر مهمی در جایگزینی سوختهای فسیلی و کاهش تولید CO2 در اتمسفر داشته باشد. آنزیم Cel6B از باکتری گرمادوست Thermobifidia fusca، یک CBHII متعلق به خانواده بی سلولازهاست که تا C° 55 مقاوم به حرارت است....

Removal of selectable marker genes (SMGs) from genetically engineered (GE) plants is desirable because of the concerns about their probable negative effects on human health, other organisms and the environment. Different strategies are used to remove SMGs from GE plants including genome editing technologies. In this study, neomycin phosphotransfera...

The Cel B enzyme from Thermobifidia fusca, a CBHII, belongs to the family of cellulase B, which is highly resistant to heat. Due to the low level of production of this enzyme in this host, it cannot be used at industrial scale. For expression of cellobihydrolase in yeast, cel B gene in pSZ plasmid was amplified by PCR and then was cloned in to pPIC...

Background Salinity is a major abiotic stress that limits the growth, productivity, and geographical distribution of plants. A comparative proteomics and gene expression analysis was performed to better understand salinity tolerance mechanisms in chickpea. Results Ten days of NaCl treatments resulted in the differential expression of 364 reproduci...

Male sterility (induced or natural) is a potential tool for commercial hybrid seed production in different crops. Despite numerous endeavors to understand the physiological, hereditary, and molecular cascade of events governing CMS in cotton, the exact biological process controlling sterility and fertility reconstruction remains obscure. During cur...

Background: In consideration for the increasing widespread use of genetically modified (GM) crops, one of the important issues for assessment is the effect of GM crops on soil microbial communities. Objectives: In this study, T2 chitinase-transgenic cotton (line #57) and its non-transgenic line were investigated for bacterial and fungal dynamics...

There is an urgent need to develop novel drugs for osteoporosis which occurs due to estrogen deficiency. Phytoestrogens derived from medicinal plants would be the best alternative to chemical drugs with harmful side effects. The main purpose of the present study was to investigate the effect of ferutinin compared to 17β-estradiol (E2) on bone miner...

There is a growing body of the literature that recognizes the importance of ferutinin (C22H30O4) as one of the natural phytoestrogens with potency to treat osteoporosis and some kind of cancers. One of the greatest challenges is availability of ferutinin that is found just in root of some plants of the genus Ferula (Apiaceae), which is the reason o...

Objective(s) The purpose of this study was to evaluate variations in yields, volatile composition and biological activities of essential oils (EOs) obtained from the aerial parts of Zhumeria majdae collected from five localities of the south of Iran. Materials and Methods The EOs were analyzed using gas chromatography and gas chromatography-mass s...

Oxidative stress is one of the serious biochemical consequences of environmental stresses, particularly salinity. The present study was conducted to evaluate biochemical, antioxidative enzymes and the corresponding gene expression responses of four contrastive chickpea genotypes to 100 mM NaCl stress during 12 days. The salinity stress imposed sign...

Genetic engineering plays an important role in modern cotton breeding research. Despite there are many achievements in cotton gene transformation studies, several limitations still exist in achieving cotton transformation the limitations of the used explant types are of low regeneration rate and genotype-dependence, limiting to application in selec...

Cytoplasmic Male Sterility is maternally inherited trait in plants, characterized by failure to produce functional pollen during anther development. Anther development is modulated through the interaction of nuclear and mitochondrial genes. In the present study, differential gene expression of floral buds at the sporogenous stage (SS) and microspor...

Integration of data mining human genome as one of the modern science with Islamic worldview has a significant role in the salvation of mankind. Information of 23 pairs of chromosomes in the nucleus of each 3.72× 1013 cells in the human body is huge database that worldwide scientists have achieved only a small part of valuable information on them. I...

The influenza A virus is of global concern for the poultry industry, especially the H5 subtype as it has the potential to become highly pathogenic for poultry and mankind. Recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. The goal of the present study was to investigate the possibility...

An Agrobacterium-mediated transient gene expression assay was carried out in alfalfa (Medicago sativa) leaves for expression of a chimeric gene encoding a part of capsid protein of Foot and Mouth Disease virus called VP1. The plant leaves were transformed via agroinfiltration procedure. The presence of the foreign gene and its expression in transfo...

The impending influenza virus pandemic requires global vaccination to prevent large-scale mortality and morbidity, but traditional influenza virus vaccine production is too slow for rapid responses. In this study, bacterial system has been developed for expression and purification of properly folded HA1 antigen as a rapid response to emerging pande...

Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to develop a recombinant vaccine, a plant express...

Salinity is a major abiotic stress, which reduces productivity of a broad range of crops, especially legumes. This study provides a comparative overview of physiological responses and expression patterns of critical genes in chickpea genotypes during five time courses of NaCl treatment. Considering lipid peroxidation (MDA), electrolyte leakage, pro...

Complex flowers containing large numbers of petals are especially prized as ornamental plants. Double-flowered Tricyrtis macranthopsis, in which stamens and carpels are converted to petaloid organs, is related to AGAMOUS (AG) gene expression. So we isolated AGAMOUS gene from T. macranthopsis, named as TrimAG. Since TrimAG expression was limited to...

Foot and Mouth Disease (FMD) is a very dangerous livestock disease which causes a serious loss in the production of milk and meat. Therefore, producing an effective recombinant subunit vaccine virus this disease is of great importance. Transient gene expression is a valuable tool to reach rapid and acceptable recombinant vaccine. An Agrobacterium-m...

1. Background Foot-and-Mouth Disease Virus (FMDV) is a causative agent of foot and mouth disease, a highly contagious disease adversely affect-ing milk and meat-producing animals around the world, particularly in developing coun-tries (1). The G-H loop of VP1 capsid pro-tein of FMDV, covering residues 134-158, has been reported by many researchers...

Fifteen non-aflatoxigenic strains of Aspergillus flavus, representing a wide range of geographic regions of Iran (six provinces including Fars, Ardebil, Guilan, Golestan, Kerman and Semnan) and vegetative compatibility groups (VCGs) were collected from corn (Zea mays L.), peanut (Arachis hypogaea L.) and pistachio (Pistachia vera L.) soils and kern...

A chimeric construct of foot and mouth disease virus capsid protein VP1 was expressed in Chlamydomonas reinhardtii via Agrobacterium-mediated genetic transformation. Integration of the foreign gene in nuclear genome of the algae was confirmed by PCR and expression of the recombinant protein was confirmed at translational level. Results showed that...

During 2012, soil samples from commercial pistachio orchards in three major production regions include Rafsanjan (Kerman Province, center of Iran), Damghan (Semnan Province, north-central Iran) and Feyz-Abad (Khorasan-e Razavi Province, northeastern Iran), were assayed on Dichloran Rose-Bengal Chloramphenicol agar (DRBC) and Aspergillus flavus-para...

One hundred-thirty soil isolates of A. flavus were collected from commercial pistachio orchards in two main production regions including Rafsanjan (Kerman Province, south-eastern of Iran) and Damghan (Semnan Province, central north of Iran) and assayed for vegetative compatibility groups (VCGs). The sixteen and twenty VCGs were identified for 41 an...

Out of fifty-two Iranian nonaflatoxigenic strains of Aspergillus flavus, collected from various substrates (soil and kernel) and sources (peanut, corn and pistachio), fifteen representatives were selected according to their different geographical origins (six provinces: Guilan and Golestan, Ardebil, Fars, Kerman and Semnan) and vegetative compatibi...

The type and amount of lipids produced by an algal species directly influence the quality of the achieved biodiesel. This study is the first to report on the isolation process and lipid profile analysis of algal strains obtained from the Persian Gulf as well as 9 previously introduced strains. Biomass productivity and lipid productivity seemed to b...

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