Saeed Nazifi

Shiraz University · School of Veterinary Medicine

The occurrence of oxidative stress (OS) and the acute phase response (APR) play crucial roles in the development of coccidiosis, thereby contributing to the pathogenicity of coccidia. This review article describes the occurrence of OS and APR in animal coccidiosis.

Background Dromedary camels (Camelus dromedarius) are raised in extremely strict ecological conditions of deserts. Camels are vulnerable to many zoonotic infections. There are limited data on the occurrence of Q fever and borreliosis in camels, in Iran. Aims The current study was focused on the occurrence of Coxiella burnetii and Borrelia spp. inf...

Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment is seemed essential. In this study, we evaluated the anti-tumor effects of nisin A and NDV on triple-negative MDA-MB-231 cell lines. MDA-MB-231 cell lines were subjected to the different concentrations of a Vero-adapted NDV (JF820294.1) and n...

The aim of this study was to investigate gonadectomy stress, steroid hormones and serotonin in male dogs treated with melatonin before gonadectomy. Twenty-five mixed breed adult dogs were divided into five equal groups. The melatonin and mela- tonin + gonadectomized groups received melatonin treatment (3 mg/10 Kg, PO, TID) the day before gonadecto...

Citral is the main ingredient of the lemongrass plant with anti-inflammatory properties. In this study, the effects of citral on reducing inflammation in experimental diabetes in rats were investigated. Forty rats were randomly divided into four groups. There were two control groups (healthy controls (H) and citral alone-treated control (HC)) and t...

Background As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce oxidative stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In th...

Purpose: Ulcerative colitis (UC) is a lifelong disease with unclear etiology and increasing prevalence worldwide. Autophagy has been reported to play roles in the pathogenesis and progression of UC. Here, we aimed to analyze the expression of autophagy related protein 10 (ATG10) and its regulator, micro-RNA (miR) 519a, in UC patients. Methods: T...

The present study investigated the effects of Shilajit extract on aspirin‐induced gastric lesions in rats. We evaluated macroscopic and histopathological lesions in the stomach, measured the activity of oxidative stress enzymes in gastric tissue homogenates, and assessed serum electrolytes and parameters of kidney and liver function. Forty‐five mal...

Background: Probiotics may improve milk quality and the general health status of animals. Aims: The effects of dietary Bacillus coagulans PRM101 on milk components, milk fatty acids (FA), and some health indicators of dairy cows were investigated. Methods: The probiotic was added to the feed of 12 Holstein cows (2 g/cow: 2 × 1011 CFU/cow) for...

Ischemia/reperfusion (I/R) injury in cadaveric liver transplantation is not avoidable. Liver I/R injury is an important phenomenon in hepatic damage. MicroRNA-21 (miR-21) plays an important role in I/R injury. The present study aimed to determine the expression pattern of miR-21 in liver I/R injury/recovery and its correlation with the immunologic...

Background: As one of the most common surgeries performed in veterinary medicine, ovariohysterectomy (OHE) can induce stress in dogs. The antioxidant properties of melatonin have been confirmed in various studies. This study aimed to investigate the effects of melatonin administration on oxidative stress in dogs before and after OHE. In this study,...

Apelin is a new adipocytokine that acts as an endogenous hormone in various tissues through its receptor (APJ). This study aimed to investigate the effects of oral administration of L-carnitine (LC) on the expression of Apelin and APJ in adipose tissue of experimentally induced insulin-resistant and type 2 diabetic rats. In this experimental study,...

Ultrasonography is one of the most common methods for the diagnosis of prostate disorders, such as benign prostatic hyperplasia (BPH), in dogs. Changes in the echotexture are one of the indicators used to diagnose prostate disorders. The purpose of this study was to investigate the changes occurred in the dogs’ prostate echotexture during the i...

Background: Multiple-organ failure is the main cause of death in diabetes mellitus and hyperglycaemia induced oxidative stress is responsible for major diabetic complications including nephropathy. Medicinal plants with antioxidant activity may modulate oxidative status and improve the functions of multiple organs that affected by chronic hypergly...

Purpose Dexamethasone has been widely used to treat acute inflammatory diseases and endotoxic shocks in animal models. Meloxicam is one of the most commonly used anti-inflammatory agents in avian species. However, little is known about the effects of dexamethasone and meloxicam on lipopolysaccharide (LPS)-induced acute inflammatory response in bird...

Melatonin has been used perioperative to control pain, inflammation, and oxidative stress. The present paper aims to evaluate the effect of the administration of melatonin on acute phase proteins and inflammatory cytokines and compare the trends after the melatonin administration in intact and castrated dogs. Twenty healthy mature male dogs (1-3 ye...

One hundred and twenty one-day-old chukar partridges were randomly divided into eight groups which received diets with different supplementations. There were four unchallenged groups. One group received salinomycin (50 ppm), two groups received cinnamaldehyde (CINN) (100 and 200 mg/kg of diet), and another one received only the basal diet from the...

A wide range of physiologic and metabolic changes occur during late pregnancy to parturition and lactation because of increased fetal growth and colostrum and milk production. This study was aimed to investigate the changes of acute-phase proteins (APPs) during important physiological condition (late pregnancy and early lactation) in dairy Saanen g...

BACKGROUND: Many additives have been recommended to calf feed but the effects of each supplement have been under investigation especially during weaning stress. OBJECTIVES: The effects of dietary supplementation of probiotic (Protexin) and chromium-methionine che- late (Cr-Met) on triiodothyronine (T3), thyroxine (T4), total protein, albumin, zinc,...

Salmonella infections have become a major health concern in recent decades. This pathogen has evolved to become resistant to antibiotics, which has caused problems in its treatment. As such, finding a novel preventive method is important in the treatment and management of this infection. In recent years, uses of probiotics, especially spore-former...

Background/aims: Ulcerative colitis (UC), along with Crohn's disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC. Methods...

The application of high-intensity ultrasound waves has been investigated as one of the non-invasive methods of contraception in several species. This study was aimed to investigate the mechanism of contraception in dogs using ultrasound waves by measuring acute-phase proteins and oxidative stress indices. Ten mixed-breed adult and fertile dogs were...

The genus Bartonella is comprised of Gram-negative coccobacilli, aerobic, and facultative intracellular bacteria which are transmitted by hematophagous vectors (e.g., fleas, lice, sandflies, and ticks). Each species of Bartonella infects one or few related mammals as reservoir host(s). If a Bartonella spp. infects a nonspecific host like humans, it...

One of the narrow-clawed crayfish of the freshwater is named Astacus leptodactylus. As the only crayfish species native to Iranian waters, Astacus leptodactylus is commonly distributed in Iranian water resources. Crayfish is considered a suitable biological indicator of the quality of water, a measure for the assessment of ecology and toxicology (e...

The monitoring of serum prostatic biomarkers during the treatment will help clinicians to know the statement of the response to finasteride in dogs affected by benign prostatic hyperplasia (BPH). The present study was aimed to assess changes in the serum canine prostate-specific esterase (CPSE), prostate-specific antigen (PSA), prostatic acid phosp...

This study aimed to evaluate the effect of two doses of cyclohexane extract of walnut leaves on total homocysteine, lipids profiles, and indices of oxidative stress including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in diabetic rats. Diabetes was induced by a single intraperitoneal (IP) inj...

Background: Prostatic hyperplasia (PH) is one of the most important disorders in intact dogs. In this study, we aimed to induce PH experimentally using the combination of testosterone and estrogen and evaluate important factors associated with this disease. Results: The results showed that in the induction group, prostate volume and prostate spe...

The aim of this study was to investigate the effect of Lactobacillus plantarum and Bacillus coagulans on serum lipid profile and lowering potential of probiotic in hypercholesterolemic rats. Twenty-eight male Wistar rats were divided into four groups as follows: (1) control group, fed standard commercial diet; (2) HC group, fed high-cholesterol die...

The study compares the effect of one-time administration of nonsteroidal and/or steroidal anti-inflammatory combinations by topical or intramuscular (IM) routes to pigeons with monosodium urate (MSU)-induced arthritis. Forty-five adult domestic pigeons were assigned into nine equal groups: NC, negative control; PC, positive control with arthritis;...

Hemotropic mycoplasmas (haemoplasmas) are wall-less bacteria, which may lead to anemia and, even, mortality in mammals. The present study was aimed to characterize the causative agent(s) of haemoplasma infection in blood samples taken from horses (n = 133) in south of Iran. Microscopic examination of blood smears and PCR assay were performed for th...

Background: Finding new agents for prevention and/or treatment of benign prostatic hyperplasia (BPH) especially from natural sources is a demanding field. Objectives: This study aimed to evaluate the effect of black mulberry (BM) (Morus nigra) fruit hydroalcoholic extract on the establishment of BPH in rats. Materials and methods: Forty-nine a...

Backgrounds: Melatonin has significant antioxidant and hepatoprotective effects in normal and oxidative stress conditions. The aim of the present study was to assess the effects of melatonin on antioxidant, hepatic, and renal factors in intact and castrated dogs. Twenty male mixed-breed adult dogs were aligned in an experimental randomized and con...

Objective: To monitor serum inflammatory cytokines during induction of benign prostate hyperplasia in dogs. Methods: This research was designed as a case-control study. There were 20 adult mixedbreed intact male dogs, which were divided into the normal group (n=10) and the benign prostate hyperplasia group (n=10). In the benign prostate hyperplasia...

New methods are being developed for the treatment of benign prostatic hyperplasia (BPH) in dogs. The aim of the present study was to evaluate the effects of Tadalafil on the treatment of experimentally induced BPH in dogs. Twenty-five adult intact male dogs were randomly divided into five groups (n=5): normal group; dogs induced with BPH and treate...

The present research was carried out to investigate the influence of mumie aqueous extract on aspirin-induced gastric lesions in rats. Thirty-eight male animals were grouped as the following: Normal control received 1 ml normal saline; positive control received 1 ml normal saline; standard control received omeprazole (20 mg/kg); treatment received...

Management of pain by different therapeutic agents has always been one of the most important components in clinical veterinary care. Concerns about various side effects of analgesics drugs have lead the pain relief to combination drug therapy. The present study was performed to evaluate the effect of combined tramadol and meloxicam on oxidative str...

Background: Finding a medical treatment which can combat cell proliferation and relax smooth muscles in canine benign prostatic hyperplasia (BPH) appears to be imperative. Aims: This study aimed to evaluate the oxidative stress and inflammatory proteins following the treatment of dogs induced for BPH with an anti-proliferative agent called tadal...

In this study, the fate of corpus luteum was investigated in cows affected by severe clinical endometritis. Also, concentration of IL-1β, IL-6, COX-2, adiponectin, leptin, progesterone (P4), PGFM, insulin, and IGF-1 were studied in severe clinical endometritis cows at day 30 ± 3 postpartum. Eighty-seven dairy cows affected by severe clinical endome...

Metabolic status of dairy cows influences reproductive tract and fertility in postpartum period. A study with 89 estrus dairy cows was performed to study the relationships among the concentration of metabolic hormones and ultrasound traits of genital system in heat dairy cows. Cows in estrus were examined with transrectal ultrasonography and palpat...

Purpose Leishmaniasis, as one of the most important vector-borne and zoonotic diseases, can be seen in different forms and is more prevalent in developing countries worldwide. Due to the absence of effective strategies in its prevention, treatment, and control, investigation of effective control strategies against the disease is necessary. In this...

Background Melatonin regulates metabolism and metabolism related hormones in mammalians. Castration has some adverse effects on the metabolic hormones of dog. This study was conducted to determine the effects of oral melatonin administration on metabolic hormones, as well as to compare changes of these hormones after administration of melatonin in...

Abstract The purpose of the study was to determine the effect of dietary iron on distribution of zinc, manganese, copper, calcium and magnesium in the body of Wistar rats. Commercial Sangak, an Iranian traditional flat bread was used in this study. It was prepared by additionof yeast and sourdough. Different doses of iron (35, 70, 140, and 210 mg/...

Application of antibiotics in livestock production has been a big concern in the recent years since they can have a vital role in the emergence of antibiotic resistant bacteria. Therefore, scientists and nutritionists have been searching for alternative agents to alter ruminal metabolism and improve feed efficiency and animal productivity. In this...

Activation of hepatic stellate cells by free radicals is an initial step in the development of liver fibrosis. Zataria multiflora Boiss (ZM) essential oil as a natural product has antioxidant activity and maybe a suitable candidate for treatment or prevention of the disease. Thus, this study aims to evaluate the protective effect of ZM oil in CCl4...

This study was performed to evaluate the effect of starvation and transportation on hematological parameters and serum concentrations of galanin, triglyceride, total cholesterol, HDL and LDL-cholesterol in goats. On the first day, first and second blood samples from ten Cashmere (Raini) goats (5 males and 5 non-lactating and non-pregnant females) w...

Colon cancer is the third most common human malignancy and a main cause of death worldwide. The current study was carried out to investigate the effects of cannabidiol, a cannabinoid, on angiogenesis and cell death in mice with experimental colon cancer induced by injection of CT26 cell line. Fifty male BALB/c mice were assigned randomly to five st...

Objective: To examine differences in the metabolite, steroid and lipopolysaccharide of follicular fluid collected from the ovulatory follicle of Holstein repeat breeding cows, lactating cows, and virgin fertile heifers. Methods: Estrus was induced in animals possessing functional corpus luteum by intramuscular administration of prostaglandin F2 α....

To investigate the effects of ginger extract on renal toxicity of silver nanoparticles, 48 male mice were randomly divided into four experimental groups; control, ginger, silver nanoparticles, and ginger plus silver nanoparticles and treated for 35 days via intraperitoneal (IP) injection of ginger (200 mg/kg) and/or silver nanoparticles (500 mg/kg)...

The pharmaceutical use of quercetin is limited due to the problems such as low solubility, bioavailability, permeability, and instability. High doses of quercetin show toxic effects in clinical and experimental studies. Therefore, a method is needed to overcome these problems without the use of toxic doses. Iron oxide nanoparticles can be used as a...

Monitoring of changes in acute phase proteins following the treatment of endometritis can help us to improve treatment protocols. In total, 81 dairy cows affected by clinical endometritis were selected in 30 days in milk (DIM) and treated with hyperimmune serum. Cervical mucus cytology was used to determine the neutrophil percentage. Levels of amyl...

The authors regret that in the Materials and methods section, on page 28, line 21, the following sentence “…. not castrated and received oral melatonin capsule.” should read as “…not castrated and did not receive oral melatonin.” The authors would like to apologize for any inconvenience caused.

Agricultural pesticides are the most important polluted pond ecosystem. The purpose of this study was examining the impact of the location of fish ponds and water source on the health of farmed fish by measuring biochemical parameters, hematological and oxidative. A total of 66 blood samples were taken from three rainbow trout farms in Fars provinc...

Clostridium perfringens type A food-borne poisoning is often caused by C. perfringens enterotoxin (CPE) encoded by chromosomal cpe. Contamination of meat with C. perfringens usually leads to food poisoning outbreaks. To find more information regarding the causative agent, we focused on the identification of type A containing cpe and netB genes in c...

Objective: L-carnitine (LC) has been shown to protect cardiac metabolism in diabetes patients with cardiovascular diseases (CVDs). Apelin, a newly discovered adipocytokines, is an important regulator of cardiac muscle function; however, the role of the level of expression of Apelin axis in improvement of cardiac function by LC in diabetic patients...

BACKGROUND: Different breeds of horses have distinct and specific metabolic profiles which reflect the health status of horses. OBJECTIVES: The present study was performed to determine the circulating metabolic biomarkers and their correlations in Darehshori horses. METHODS: Blood samples were taken from 56 Darehshori horses (5 to 7 years old). The...

Background: Quercetin (QT) as a bioactive flavonoid has a potential therapeutic activity for numerous neuronal injuries and neurodegenerative diseases. However, the low absorption rate of QT, especially through the blood-brain barrier, restricts its bioactivity in the body. The current research took the advantage of superparamagnetic iron oxide na...

The subclinical mastitis can cause economical loss, therefore, access to the methods of prevention, control and reduction of mastitis, especially subclinical form, is so important and valuable. The aim of this study was to investigate the changes of adenosine deaminase enzyme activity in the cow milk with subclinical mastitis. Forty-six cows with s...

Normal cyclic changes in hormonal and ultrasonic echotexture along with estrus are critical for successful breeding in dairy cows. We studied relationships among steroid hormone concentrations and ultrasound changes of uterus and ovaries just before insemination in heat dairy cows. Eighty-nine estrus dairy cows were selected for transrectal examina...

چکیده1 هدف از مطالعه حاضر بررسی اثر موقعیت استخرهاي پرورش ماهی بر سلامت ماهیان پرورشی با توجه به فاکتورهاي خونی آن ها بود. لذا استخرهاي سه منطقه انتخاب شد که از نظر موقعیت جغرافیایی و فاصله از زمین هاي کشاورزي متفاوت بودند. منطقه یک، منبع آب استخر از رودخانه، منطقه دو از چاه و منطقه سه از آب چشمه بود. کشاورزان منطقه یک بیشترین مقدار سموم دفع آفات ک...

The most significant postoperative complication of spaying is bleeding from ovarian pedicles. In this study, all of the ovarian vessels were bilaterally ligated; then, ovaries were stimulated by gonadotropins. In a clinical trial and sequential control experiment, five healthy mixed-breed mature female dogs were randomly selected and kept under the...

Objective: To confirm the efficacy of non-antibiotic treatment with prostaglandin F2 α (PGF2 α) in dairy cows affected by severe clinical endometritis in (30±3) days in milk. Methods: Cows with clinical endometritis 1 (n=399) were aligned into three groups randomly. The first group (n=115) received PGF2 α, the second group (n=84) received intrauter...

Melatonin is a regulatory hormone that orchestrates many responses to changes occurring in the body and environment. In the present study, melatonin was administrated immediately after castration to 20 male dogs (3 mg/10 Kg, Per os or orally, four groups [n = 5]: melatonin, castrated, castrated + melatonin, and control) for 30 days. Blood samples w...

So far, a number of hemotrophic mycoplasma (HM) species have been reported from different animals, mostly based on the 16S rDNA gene sequence analysis. Although previous studies have described Candidatus Mycoplasma haemolamae from alpaca (Lama pacos), llama (Lama glama), and vicuna (Lama vicugna) in Camelidae, data on the causative agent(s) of hemo...

Background: Multiple-organ failure is the main cause of disability and death in diabetes mellitus. Imbalance in oxidative status is responsible for major diabetic complications and multiple-organ failure including blindness. Recently, plants with potential antioxidant properties have been used to manage hyperglycemia in diabetes. This study was und...

Background: Radio frequency wave (RFW) generated by mobile phones and wireless communication systems has been reported to cause adverse effects on reproductive function, vision and hematological parameters, possibly through oxidative stress. The aim of this study was to evaluate the effect of RFW generated bybase transceiver station BTS on oxidativ...

Limited information is available for normal biochemical reference values in captive Persian wild goats (Capra aegagrus). The objectives of this study were to establish reference values and to evaluate changes associated with gender and age for 22 mature, captured, clinically healthy Capra aegagrus. The reference values were obtained: glucose, 34.10...

Background Superparamagnetic iron oxide nanoparticles (SPION) have been largely considered for numerous applications in biomedicine such as magnetic resonance imaging, hyperthermia, cell tracking, anticancer treatment, and targeted delivery of drugs or genes. However, they may have side effects such body weight loss. Quercetin (QT), a strong antiox...