Saeed Aminzadeh

National Institute of Genetic Engineering and Biotechnology | NIGEB · Department of Industrial and Environmental Biotechnology

Cyanide compounds are hazardous compounds which are extremely toxic to living organisms, especially free cyanide in the form of hydrogen cyanide gas (HCN) and cyanide ion (CN−). These cyanide compounds are metabolic inhibitors since they can tightly bind to the metals of metalloenzymes. Anthropogenic sources contribute significantly to CN− contamin...

Cellulases are hydrolytic enzymes with wide scientific and industrial applications. We described a novel cellulase, CelC307, from the thermophilic indigenous Cohnella sp. A01. The 3-D structure of the CelC307 was predicted by comparative modeling. Docking of CelC307 with specific inhibitors and molecular dynamic (MD) simulation revealed that these...

Lispro is an insulin analog with an accelerated action profile suitable for post-prandial injections and glycemic control in diabetes. Insulin production in Escherichia coli leads to inclusion body formation due to its reducing inner environment. This conventional platform requires refolding procedures with relatively low recovery resulting in prod...

A new strain of Influenza A Virus (IAV), so-called "H7N9 Avian Influenza", is the first strain of this virus in which a human is infected by transmitting the N9 of influenza virus. Although continuous human-to-human transmission has not been reported, the occurrence of various H7N9-associated epidemics and the lack of production of strong antibodie...

This study describes the hyaluronic acid (HA) production by S. zooepidemicus ATCC 43079, and the effect of the hyaluronidase enzyme on HA levels. The hyaluronidase production, glucose consumption, and lactate formation were recorded during fermentation. The HA production, and productivity at different amounts of glucose, yeast extract and pH were e...

As a safe substitute for hydroquinone, β-arbutin, a natural plant substance, and its synthetic counterpart, α-arbutin, are used in depigmentation formulations. However, there are debatable points regarding the impact of arbutin on tyrosinase and the pigmentation process. To shed light on this issue, the effects of Pyrus biossieriana leaves extract...

The biodetoxification of cyanide-rich wastewater has been suggested as an appropriate technique due to its environmental friendliness and cost effectiveness. In this research, Enterobacter zs that was newly isolated from cyanide-polluted wastewater was selected to catalyze cyanide via an enzymatic mechanism. Enzyme was purified and its activity was...

Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast period of 2020–2025. Nonetheless, the large-scale POX preparation still relies on the extraction from agricultural products, while there is an accumulative driving force toward employing biotechnological processes with agricult...

Superoxide dismutases (SODs) (EC 1.15.1.1) are well known antioxidant enzymes that play critical roles in cellular defenses of living organisms against harmful superoxide radicals during oxidative stress. This study details on cloning, biochemical and functional characterization of an iron containing type superoxide dismutase (SOD) from a novel the...

Thermostability and substrate specificity of proteases are major factors in their industrial applications. rEla is a novel recombinant cysteine protease obtained from a thermophilic bacterium, Cohnella sp.A01 (PTCC No: 1921). Herein, we were interested in recombinant production and characterization of the enzyme and finding the novel features in co...

Characterization and identification of bacteria and microorganisms are crucial in several fields such as medical, agricultural, and industrial microbiology. Conventional phenotype-based identification methods use low-precision scoring systems and therefore are affected by species phenotypic variations. These methods have low levels of reproducibili...

Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast period of 2020 - 2025. Nonetheless, the large-scale POX preparation still relies on the extraction from agricultural products, while there is an accumulative driving force toward employing biotechnological processes with agricu...

Objectives Extensive applications of peroxidase (POX) have raised the global market demand at a considerable rate during the forecast period of 2020 - 2025. Nonetheless, the large-scale POX preparation still relies on the extraction from agricultural products, while there is an accumulative driving force toward employing biotechnological processes...

Chitinase is one of the essential enzymes that have broad applications in industrial, agriculture, medical through biodegradation of chitin and hence are in demand. Chitinase-producing Serratia marcescens B4A was cultured in the medium containing chitin and then was purified through ammonium sulfate precipitation and DEAE ion-exchange chromatograph...

Proteases play an essential role in living organisms and represent one of the largest groups of industrial enzymes. The aim of this work was recombinant production and characterization of a newly identified thermostable protease 1147 from thermophilum indigenous Cohnella sp. A01. Phylogenetic tree analysis showed that protease 1147 is closely relat...

The prepared protein and protein-ligand complexes (protease-SDS and protease-tween complexes) were used in MD simulation, performed using GROMACS v 4.6.5 with CHARMM36 all-atom force field. The CGenFF server (https://cgenff.umaryland.edu/) provides topologies and parameters of ligands compatible with the CHARMM36 all-atoms force field. Protein and...

After the cultivation period, cells were collected by centrifugation at 3500 × g for 30 min at 4 °C and resuspended in 4 ml lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM Imidazole and 0.05% Tween 20 at a pH of 7). The mixture was sonicated with 4 × 40 sec pulses followed by 20 sec rest between cycles at 4 °C. The crude extracts were then centrifu...

The nucleotide sequence of Coh01147, previously obtained from Cohnella sp. A01 (Genbank Accession No: QEV88423) was translated to the amino acids using Expasy translate tool (http://web.expasy.org/translate). The resulting protein sequencewas used for further in silico analysis of the Cohnella sp. A01 derived protease 1147 protein. The biochemical...

L-glutaminase importance to use in the food industry and medicine has attracted much attention. Enzymes stability has always been a challenge while working with them. We heterologously expressed and characterized a novel stable L-glutaminase from an extremophile bacterium (Cohnella sp. A01, PTCC No: 1921). Km, Vmax, catalytic efficiency and specifi...

There is an increasing need for accurate and inexpensive glucometers as the world moves toward personalized medicine. Among the existing technologies, photometric based devices are more desired due to the cost-effectiveness, ease-of-use and the potential to be adopted in the smart-phone technology for remote sensing and self-monitoring purposes. Ho...

Laccase (EC 1.10.3.2; benzenediol; oxygen oxidoreductases) is a multi-copper oxidase that catalyzes the oxidation of phenols, polyphenols, aromatic amines, and different non-phenolic substrates with concomitant reduction of O2 to H2O. Enzymatic oxidation techniques have the potential of implementation in different areas of industrial fields. In thi...

Chitinases with high thermostability are important for many industrial and biotechnological applications. This study was conducted to enhance the stability of Serratia marcescens B4A chitinase by site directed muta-genesis of G191 V. Further characterization showed that the thermal stability of the mutant showed marked increase of about 5 and 15 fo...

Background: Typically, non-cellulytic glucanase, including fungi and yeast cell wall hydrolyzing enzymes, are release by some symbiotic fungi and plants during the mycoparasitic fungi attack on plants. These enzymes are known as the defense mechanisms of plants. This study intends to investigate the biochemical properties of β-1,6-glucanase (bg16M...

Citrus canker is a citrus disease species created by the bacterium Xanthomonas citri subsp.citri. Many citrus, such as oranges, lime, and grapefruit are affected by the infectious bacteria and stems, leaves and fruits are experiencing loss. In this experiment antibacterial effects of five kinds of antibiotics including ampicillin, kanamycin, chlora...

Cyanide is used in many industries despite its toxicity. Cyanide biodegradation is affordable and eco-friendly. Sampling from cyanide-contaminated areas from the Muteh gold mine and isolation of 24 bacteria were performed successfully. The selected bacteria—‘Bacillus sp. M01’—showed maximum tolerance (15 mM) to cyanide and deposited in Persian Type...

Insulin as a small molecule with 51 residues is an interesting model useful in studying protein misfolding of neurodegenerative amyloid proteins. Investigating zinc effects on insulin misfolding and aggregation triggered by 80% ethanol is the main objective of the present work. Using different methods of turbidity measurement, examining thioflavin...

Membrane proteins play a key role to understand the complex interactions that allow for unicellular and multicellular life up to humans. In this review we are going to describe the procedure of generating of modern medicine drugs. First of all, a specific membrane protein which has a key role in one or more diseases is selected. Next, the gene of t...

Tyrosinases, laccases, and peroxidases are three important groups of oxidative enzymes. A characteristic feature of these enzymes is their ability for the oxidation of aniline and phenolic compounds. Literature review reveals some overlap between the organic substrates of these enzymes, but how exactly they are different and how much they have in c...

Some industries require newer, more efficient recombinant enzymes to accelerate their ongoing biochemical reactions in harsh environments with less replenishment. Thus, the search for native enzymes from extremophiles that are suitable for use under industrial conditions is a permanent challenge for R & D departments. Here and toward such discoveri...

Table A in S1 File: Bacterial α-amylases and amylopullulanases used for multiple sequence alignment and phylogenetic tree. Table B in S1 File: The % identity of proteins from this study with other Amylopullulanase. Table C in S1 File: Molecular weight, pH optimum, temperature optimum and Kinetic parameters of some amylopullulanases from GH13 and GH...

Increasing demand for efficient and environmentally benign oxidation technologies has resulted in a focus on the use of oxidoreductases. Laccases and tyrosinases, which utilize molecular oxygen and produce water as by-product, are particularly attractive. Simultaneous production of laccase and tyrosinase was studied in Neurospora crassa FGSC #321 a...

Thermostable chitinases are useful for industrial and biotechnological applications. This paper reports the stabilization of chitinase from Serratia marcescens B4A through rational mutagenesis. Changing of Ser 390 to Ile in S. marcescens. The stabilization was enhanced through entropic stabilization by reduction of the loop length and also by incre...

Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3) are one of the highest value commercial enzymes as they have potential applications in biotechnology for detergents, food, pharmaceuticals, leather, textiles, cosmetics, and paper industries; and are currently receiving considerable attention because of their potential applications in biotechnolog...

Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiologi...

Background: The rapid increase of HIV-1 strains resistant to current antiretroviral drugs is a challenge for successful AIDS therapy. This necessitates the development of novel drugs, and to this end, availability of screening systems for in vitro drug discovery is a priority. Herein, we report the modification of a previously developed system for...

Background: Xanthomonas citri subsp. citri (Xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. Varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins. Objectives: Here and for the first time, we report the isolation and characterization of two...

Secondary structure content of proteins in molten globule state is relatively constant while the quantity of tertiary structures clearly declines due to alterations in side-chain packing. In the present study, we analyze the MG state of lipase-3646 for the first time. We introduce lipase-3646 as an appropriate model for investigating the properties...

Lipases form one of the most important groups of biocatalysts used in biotechnology. We studied the lipase from the bacterium Cohnella sp. A01 due to the versatility of thermophilic lipases in industry. In this study lipase 3646 gene from the thermophilic bacterium Cohnella sp. A01 was expressed in Escherichia coli and the enzyme was purified by a...

Lipases from Bacillus thermocatenulatus are a member of superfamily of α/β hydrolase, but there are structural differences between them. In this work, we focused on the α5 helix of B. thermocatenulatus lipase (BTL2) which is absent in canonical α/β hydrolase fold. In silico study showed that the α5 helix is a region that causes disorder in BTL2 pro...

Breast cancer is a serious and potentially lethal multi-factor disease among 40-50 aged women in both developed and developing countries. Also, various studies have pointed to roles of neurotransmitters like serotonin in development of cancers, through action on various types of receptors. This study was conducted to evaluate serotonin receptor (5H...

Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum transition (Tm) and denaturation...

Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum transition (Tm) and denaturation...

Bacillus thermocatenulatus lipase 2 (BTL2), a thermoalkalophilic lipase, is the best studied enzyme for it's particular properties that has made it useful in different industries. Displacement of conserved Phenylalanine 17 residue in active site of BTL2 has a critical role in oxyanion hole-forming which is important for the enzyme activity. In this...

Citrus take over important roles in economy for every country and Iran is no exception to this rule is where the economic self-sufficiency in agriculture is the reason that the incidence of pests as possible to prevent. We have to do drastic an actions in order to their control. There are a variety of bacterial and fungal diseases that can have dev...

Citrus canker bacterial disease is one of the most devastating citrus diseases and including a quarantine disease that severe damage to citrus, which made Iran. Is that why this is necessary to study about inhibition of this disaster. Xanthomonas citri subsp.citri (xcici) is agent of this disease. In recent years, many efforts have been focused on...

Chitinases are one of the important industrial enzymes which have significant role in different industries such as digestion of chitin and chitoligosaccharides, bioremediation and control of plants pathogenic fungal as well as insects. This enzyme catalyzes the β 1→6 glycoside bond and then hydrolyses chitin polymers. chitinases consists of a (ß/α)...

Chitinases are slow-reacting but important enzymes as they are anticipated to have diverse applications. The role of a chitin-binding domain (ChBD) in enhancing the quality of binding is essential information for purposeful engineering of chitinases. The idea of making hybrid chitinases by fusing a known ChBD to a chitinase, which naturally lacks C...

Lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. To provide chimeric Bacillus thermocatenulatus lipase (BTL2), the completely conserved pentapeptide (112Ala-His-Ser-Gln-Gly116) was replaced with similar sequences (207Gly-Glu-Ser-Ala-Gly211) of Candida r...

A 12-wk experiment was conducted to study the effects of feeding regimen, inclusion of a fiber source, and CP content of the diet on performance of broiler breeder hens. In total, 360 hens and 60 males, 43 wk of age, were assigned to 60 floor pens (6 hens and 1 male each). There were 12 treatments arranged factorially with 2 feeding regimens [restr...

The effects of inclusion of cellulose or inulin as a source of dietary fiber and CP content of the diet on hepatic fat deposition were investigated in hens fed restricted or close to ad libitum consumption. There were 12 dietary treatments forming a 2 × 3 × 2 factorial with 2 feeding regimens [restricted and liberal (close to ad libitum consumption...

Chitinases have received attention because of their wide applications in the medicine, biotechnology, agriculture, waste management and industrial applications such as food quality enhancer and biopesticide. Excessive use of insecticides has led to several problems related to pollution and environmental degradation. In this study, isolation and ide...

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

A thermophile cellulase-producing bacterium was isolated and identified as closely related to Geobacillus subterraneus. The strain, named Geobacillus sp. T1, was able to grow and produce cellulase on cellobiose, microcrystalline cellulose, carboxymethylcellulose (CMC), barley straw, wheat straw and Whatman No. 1 filter paper. However, barley and wh...

Chitinases have been suggested to be involved in pathogen–antagonist interaction during biological control progress of plant pathogenic fungi. Here, a recombinant bacterial chitinase originally from Serratia marcescens B4A was produced, purified, and assayed biochemically to ascertain the activity and determine the kinetics parameters. Active enzym...

1. The objective of this experiment was to study the influences of xylanase and citric acid on the performance, nutrient digestibility, digesta viscosity, duodenal histology, and gut microflora of broilers fed on a wheat based diet. 2. The experiment was carried out as a 2 × 3 factorial arrangement with two concentrations of xylanase (0 and 200 mg/...

An experiment was conducted to study the effects of inclusion of a wheat cultivar (high in nonstarch polysaccharides) and xylanase supplementation of the diet on productive performance, pH of the gastrointestinal tract, nutrient retention, and intestinal enzyme activity of Hy-Line W-36 laying hens from 25 to 47 wk of age. The experiment was complet...

The effects of non-starch polysaccharide (NSP) content of wheat and xylanase supplementation (XS) of the diet, on intestinal enzyme activity (amylase, aminopeptidase and lipase), fat digestibility and ileal viscosity of laying hens has been studied. Two hundred forty Hy-Line W-36 layer from 20 to 25 week of age were studied under a factorial experi...

Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indi...

An experiment was conducted to study the effects of xylanase and citric acid on the performance, nutrient retention, jejunal viscosity, and size and pH of the gastrointestinal tract of broilers fed a low-P wheat-based diet. The experiment was conducted as a 2 × 3 factorial arrangement with 2 levels of xylanase (0 and 200 mg/kg) and 3 levels of citr...

Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indi...

Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indi...