Sadaruddin Chachar

Verified

Sadaruddin verified their affiliation via an institutional email.

Learn more

Verified

Sadaruddin verified their affiliation via an institutional email.

Learn more

• PhD Biochemistry and Molecular Biology
• PostDoc Position at Zhongkai University of Agriculture and Engineering

Protocorm-Like Bodies (PLBs) have emerged as promising tools for the regeneration of monocotyledonous plants, offering valuable applications in agriculture, horticulture, and conservation. Mimicking early plant embryo development, PLBs provide an efficient method for clonal propagation, enabling the large-scale production of monocots, including eco...

Tiller development is a critical factor in boosting agricultural productivity and securing global food security. This review offers a comprehensive analysis of recent advancements in enhancing crop yield through extensive research on tiller development, utilizing a multi-faceted approach that includes quantitative trait loci (QTL) mapping, associat...

Arbuscular Mycorrhizal (AM) symbiosis is integral to sustainable agriculture and enhances plant resilience to abiotic and biotic stressors. Through their symbiotic association with plant roots, AM improves nutrient and water uptake, activates antioxidant defenses, and facilitates hormonal regulation, contributing to improved plant health and produc...

Nitrogen is a critical nutrient for plant growth and productivity, but inefficiencies in its use in agriculture present both economic and environmental challenges. Enhancing nitrogen use efficiency (NUE) is essential for promoting sustainable crop production and mitigating the negative impacts of nitrogen loss, such as water pollution and greenhous...

Soil Phosphorus (P) fixation and Arsenic (As) contamination pose significant challenges to agriculture and environmental health. Biochar has emerged as a promising soil amendment capable of enhancing P availability while immobilizing As. This review explored the mechanisms by which biochar influences P dynamics and As sequestration. Biochar enhance...

Garlic (Allium sativum L.) is a vital crop, yet its growth and sprouting at the green stage can be significantly influenced by nutrient availability, which poses a challenge for optimizing yield and quality. The present research was carried out in 2022-2023 at the Nursery Department of Horticulture, Sindh Agriculture University Tandojam, to assess...

Global warming poses a significant threat to plant ecosystems and agricultural productivity, primarily through heat stress (HS), which disrupts photosynthesis, respiration, and overall plant metabolism. Epigenetic modifications, including DNA methylation, histone modifications, and RNA modifications, enable plants to dynamically and heritably adjus...

The integration of zinc nanoparticles (Zn NPs) with biochar offers a transformative approach to sustainable agriculture by enhancing plant productivity and human nutrition. This combination improves soil health, optimizes nutrient uptake, and increases resilience to environmental stressors, leading to superior crop performance. Our literature revie...

Wheat (Triticum aestivum L.) is a fundamental crop essential for both human and animal consumption. Addressing the challenge of enhancing wheat yield involves sophisticated applications of molecular genetics and genomic techniques. This review synthesizes current research identifying and characterizing pivotal genes that impact traits such as grain...

The role of modified biochar in enhancing phosphorus (P) availability is gaining attention as an environmentally friendly approach to address soil P deficiency, a global agricultural challenge. Traditional phosphatic fertilizers, while essential for crop yield, are costly and environmentally detrimental owing to P fixation and leaching. Modified bi...

Vegetable cultivation stands as a pivotal element in the agricultural transformation illustrating a complex interplay between technological advancements, evolving environmental perspectives, and the growing global demand for food. This comprehensive review delves into the broad spectrum of developments in modern vegetable cultivation practices. Roo...

Epigenetics is an expansive and rapidly evolving field, fundamentally concerned with heritable changes in gene expression that do not involve alterations to the underlying DNA sequence [...]

Anthocyanins are plant-based pigments that are primarily present in berries, grapes, purple yam, purple corn and black rice. The research on fruit corn with a high anthocyanin content is not sufficiently extensive. Considering its crucial role in nutrition and health it is vital to conduct further studies on how anthocyanin accumulates in fruit cor...

Sugarcane is grown worldwide from cultivars with complex genetic constitutions. Tillers and effective stalks are important agronomic traits of sugarcane that play important roles in yield. Using a two-way pseudo-testcross mapping strategy, we obtained a total of 172 F1 population of Yuenong73-204 (low tillering sugarcane variety) × CP72-1210 (ultra...

Magnesium (Mg²⁺) is pivotal for the vitality, yield, and quality of horticultural crops. Central to plant physiology, Mg²⁺ powers photosynthesis as an integral component of chlorophyll, bolstering growth and biomass accumulation. Beyond basic growth, it critically affects crop quality factors, from chlorophyll synthesis to taste, texture, and shelf...

Present studies were conducted on to assess the soma clonal variation in CP67-1026 mutant of sugarcane through methodological and molecular procedures. It was concluded that the Soma-clonal variation does occur in the procedure of reproduction of soma-clonal variants under invitro circumstances. Significant alterations among the sugarcane soma-clon...

Grafting is an ancient agricultural technique that is frequently used to enhance the performance of horticultural plants, including vegetables and woody fruit trees. For successful grafting, genotypes of the compatible scion (the upper part) and the rootstock (the lower part) must interact. Molecular signals, including nutritional and hormonal sign...

Climate change and water scarcity are increasing threats to global food security. In this context, it is important to identify wheat varieties that are tolerant to water stress. This study was conducted at the Department of Crop Physiology, Faculty of Crop Production, Sindh Agriculture University, Tandojam, in 2022, to evaluate the genetic diversit...

The banana cultivar 'Basrai' is a popular and economically important crop and is highly valued for its superior taste and texture. It is one of the most widespread and commonly cultivated banana varieties in Pakistan specially in Sindh province. However, the propagation of this cultivar through conventional methods using suckers is challenging and...

Persimmon (Diospyros kaki Thunb.) is an economically important fruit crop that originates from China, and has been cultivated for thousands of years. However, the genetic variation remains largely unknown. Evaluation of agronomic traits and genetic diversity in different persimmon germplasm plays an essential role in understanding of the persimmon...

Present studies were conducted on evaluation of soma-clonal variation of CP67-1026 mutant through morphological procedures with the aims at discussing how soma-clonal variations can be analyzed through morphological approaches in gaining knowledge about the underlined regulatory controlled mechanisms in plants. In vitro experiments were conducted i...

Different concentrations of nutrient media were used, amongst them; the explants cultured on MS media supplementing with IAA 2.00 mg l-1 + BAP 2.00 mg l-1 + NAA 1.0 mg l-1 acquired least time (19.55 days) to regenerate. observed at beneath the centralization of MS+ IAA 1.00 mg l-1 + BAP 2.00 mg l-1 and recorded the least days taken at benchmark (26...

Traditional plant breeding encompasses repetitive crossing and selection based on morphological traits, while phenotypic selection has been complemented by molecular methods in recent decades. Genome editing with techniques like the CRISPR-Cas9 system is still a novel approach that is being used to make direct modifications to nucleotide sequences...

Citation: Shaikh, A.A.; Chachar, S.; Chachar, M.; Ahmed, N.; Guan, C.; Zhang, P. Recent Advances in DNA Methylation and Their Potential Breeding Applications in Plants. Horticulturae 2022, 8, 562. https://doi.org/10.3390/ horticulturae8070562 Academic Editor: Jose V. Die

Drought is one of the hot topics needing urgent attention in the current era of climate change. Crop plants are frequently exposed to a range of abiotic and biotic stresses during the cropping season. Among the various abiotic stresses, drought stress is a key environmental constrain that has detrimental effect on plant growth and development. With...

The timing of floral transition is critical for reproductive success in flowering plants. In long-day (LD) plant Arabidopsis, the floral regulator gene FLOWERING LOCUS T (FT) is a major component of the mobile florigen. FT expression is rhythmically activated by CONSTANS (CO), and specifically accumulated at dusk of LDs. However, the underlying mec...

Most epithelium tissues continuously undergo self-renewal through proliferation and differentiation of epithelial stem cells (known as homeostasis), within a specialized stem cell niche. In highly innervated epithelium, peripheral nerves compose perineural niche and support stem cell homeostasis by releasing a variety of neurotransmitters, hormones...

Our era has witnessed tremendous technique advances and mechanically epigenetic improvement in plant epige-netics, mainly including histone post-translational modifications (PTMs) and DNA methylation, which have been characterized as playing vital roles in development processes and plant response to environmental factors. Recently , chemical modifi...

Epigenetic modifications alter the gene activity and function by causing change in the chromosomal architecture through DNA methylation/demethylation, or histone modifications without causing any change in DNA sequence. In plants, DNA cytosine methylation (5mC) is vital for various pathways such as, gene regulation, transposon suppression, DNA repa...

Both genetic and epigenetic information must be transferred from mother to daughter cells during cell division. The mechanisms through which information about chromatin states and epigenetic marks like histone 3 lysine 27 trimethylation (H3K27me3) are transferred have been characterized in animals; these processes are less well understood in plants...

Persimmon (Diospyros kaki Thunb.) is one of the most important fruit that is grown commercially worldwide including the East Asia and Mediterranean regions, and it has been cultivated for its fruit with high nutritional values. Phenology of fruits has attracted the interest of many breeders, growers, and researchers for accurately schedule timing o...

To observe the effect of high temperatures stresses i.e., normal (25 °C), high (30 °C) and very high (35 °C) stress, on germination and seedling growth on wheat genotypes a water culture study was conducted under controlled conditions (growth incubators). Eighteen newly developed wheat lines (DH- I, DH-3, DH-4, DH-5, DH-6, DH-7, DH-8, DH-10, DH-11,...

Germplasm conservation and evaluation has been regarded as one of the most successful methods for preserving and utilizing genetic resources in commercial crops. However, for commercial fruit crop in East Asia, persimmon (Diospyros kaki), the understanding of its genetic diversity remains largely unclear and is of great interest both for conservati...

Persimmon (Diospyros kaki Thunb.) can produce unisexual (male and female) and bisexual (hermaphrodite) flowers, showing relatively complex sexuality at the species level. In China, there are more than 1000 persimmon germplasms, but most are gynoecious cultivars that bear female flowers only. To date, few germplasms have been reported to generate ma...

To increase seed yield and oil contents, variability in breeding material is a pre-requisite. Plant material was comprised of forty-nine sunflower genotypes to investigate the variability and identification of superior genotypes by multivariate analysis. The data were recorded for ten quantitative traits; days to maturity (DM), plant height (PH), s...

The genus Diospyros contains more than 500 species, among them persimmon (Diospyros kaki Thunb.) is the most economically important species. However, the inter- and intra-specific genetic diversity remains unknown due to historic cultivation, domestication and introduction in different regions of China. In this study, we investigated the genetic di...

Epigenetic modifications including chromatin modifications and DNA methylation have emerged as essential marks that can influence various biological pathways in multicellular organisms. In plants, DNA methylation such as 5‐methylcytosine (5mC) and N6‐methyladenosine (6mA), which was discovered later, have crucial roles in the regulation of developm...

Advances in the detection and mapping of messenger RNA (mRNA) N6-methyladenosine (m6A) and 5-methylcytosine (m5C) and DNA N6-methyldeoxyadenosine (6mA) redefined our understanding of these modifications as additional tiers of epigenetic regulation. In plants, the most prevalent internal mRNA modifications, m6A and m5C, play crucial and dynamic role...

The Streptophyta include unicellular and multicellular charophyte green algae and land plants. Colonization of the terrestrial habitat by land plants is a major evolutionary event that has transformed the planet. So far, lack of genome information on unicellular charophyte algae hinders the understanding of the origin and the evolution from unicell...

Persimmons (Diospyros kaki Thunb.), originated in China, have a long history of cultivation and domestication in East Asia. In China, as one of most important fruit crops, the persimmon is utilized as fresh or dried fruit as well as an important timber and ornamental plant. There were more than 1000 germplasms and abundant morphological resources d...

In breeding programs, it is imperative to recognize the genetic variations and levels of association between traits that directly or indirectly affect the yield. Achene yield and related traits in forty-nine sunflower genotypes were studied for genetic variability, correlations and path coefficient analysis. The phenotypic coefficient of variation...

The experiment was carried out in a three replicated randomized complete block design, having net plot size of 6 x 8 m = (48 m2 ), during the year 2013-14 at the experimental fields of Cotton Section, Agriculture Research Institute, Tandojam. The treatments are comprised such as control or un-treated plots, Planofix at 50 ml/500 litres of water at...

It was concluded from the present study that macropropagation is an excellent option for producing low cost quality planting material. This is a simple method because of the ease of multiplication, saves cost of producing planting material and has the potential of producing 45-50 shoots per sucker in 4-5 months. This strategy can be easily adopted...

Pakistan is one of the most severely affected countries by Global climate change, it is an agriculture based country and its economy (21%) mainly depend on agriculture production. Wheat is the major staple food crop in Pakistan and takes key position in the national economy. It contribute 12.5% share in agriculture and 2.9% in the country's GDP. Fr...

Pakistan is one of the most severely affected countries by Global climate change, it is an agriculture based country and its economy (21%) mainly depend on agriculture production. Wheat is the major staple food crop in Pakistan and takes key position in the national economy. It contribute 12.5% share in agriculture and 2.9% in the country’s GDP. Fr...

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticum aestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the ye...

The experiment was carried out in a three replicated randomized complete block design, having net plot size of 6 x 8 m = (48 m2 ), during the year 2013-14 at the experimental fields of Cotton Section, Agriculture Research Institute, Tandojam. The treatments are comprised such as control or un-treated plots, Planofix at 50 ml/500 litres of water at...

The study was carried out to evaluate the effect of halo (KCl) priming on seed germination and early seedling growth of wheat genotypes under laboratory conditions during the year 2012, in post graduate laboratory of the Department of Crop Physiology, Faculty of Crop Production, Sindh Agriculture University Tandojam. Five wheat varieties (TD-1, J-8...

2013) Screening for salt tolerant rice (Oryza sativa L.) genotypes at early seedling stage. Journal of Agricultural Technology 10(3):779-790. Salinity is one of the major threats facing the cultivation of field crops. The response of six rice (Oryza sativus L.) genotypes to different sodium chloride levels; 0, 40, 60, 80 and100 mM were investigated...