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Publications (31)
As an efficient method to remove contaminants from highly polluted sites, enzyme biodegradation addresses unresolved issues such as bioremediation inefficiency. In this study, the key enzymes involved in PAH degradation were brought together from different arctic strains for the biodegradation of highly contaminated soil. These enzymes were produce...
Despite recent attention being paid to the biodegradation of petroleum hydrocarbons in cold environments, scale-up studies of biodegradation are lacking. Herein, the effect of scale-up on the enzymatic biodegradation of highly contaminated soil at low temperatures was studied. A novel cold-adapted bacteria (Arthrobacter sp. S2TR-06) was isolated th...
As an efficient method to remove contaminants from highly polluted sites, enzyme biodegradation addresses unresolved issues such as bioremediation inefficiency. In this study, the key enzymes involved in PAHs degradation were brought together from different arctic strains for biodegradation of highly contaminated soil. These enzymes were produced v...
A bioinspired device was fabricated as a sustainable remedial method and its performance as a membrane-enzyme reactor with cyclic ultrafiltration was investigated. The body of the jellyfish-like device was composed of two parts: 1) Jellyfish arms: Mono and co-axial electrospinning have been utilized to synthesize the flexible parts (e.g., multilaye...
Stability and reusability properties are the two most important factors that determine an enzyme's application in industry. To this end, cold-active crude enzymes from a psychrophile were immobilized on magnetic chitosan microparticles using glutaraldehyde as a linker. Immobilization was optimized based on four variables, such as magnetic particle,...
Petroleum degrading enzymes can be used as an alternative way to improve petroleum bioremediation approaches. Alcanivorax borkumensis is an alkane-degrading bacteria that can produce petroleum degrading enzymes such as alkane hydroxylase and lipase. In this study, pilot-scale Alcanivorax borkumensis fermentation was developed for producing large vo...
According to the United Nations, 2.2 billion people lack safe managed drinking water, and 4.2 billion people lack access safety managed sanitation (clean water and sanitation goal). Wastewater and drinking water treatment plants play critical roles in the urban water cycle and access to the clean water. Characterization of the pollutants is essenti...
Rapid, inexpensive, and laboratory-free diagnostic of viral pathogens is highly critical in controlling viral pandemics. In recent years, nanopore-based sensors have been employed to detect, identify, and classify virus particles. By tracing ionic current containing target molecules across nano-scale pores, nanopore sensors can recognize the target...
p-Xylene is considered a recalcitrant compound despite showing a similar aromatic structure to other BTEXs (benzene, toluene, ethylbenzene, xylene isomers). This study evaluated the p-xylene biodegradation potential of three psychrophilic Pseudomonas strains (Pseudomonas putida S2TR-01, Pseudomonas synxantha S2TR-20, and Pseudomonas azotoformans S2...
Enzymatic bioremediation is a sustainable and environment-friendly method for the clean-up of contaminated soil and water. In the present study, enzymatic bioremediation was designed using cold-active enzymes (psychrozymes) which catalyze oxidation steps of p-xylene biodegradation in highly contaminated soil (initial concentration of 13,000 mg/kg)....
Plastics use is growing due to its applications in the economy, human health and aesthetics. The major plastic particles in the form of microplastics (MPs) released into the environment are made up of polyethylene (PE), polypropylene (PP), polyvinylchloride (PVC), and polyethylene terephthalate (PET). Tremendous usage and continuous accumulation of...
Toluene/o-Xylene Monooxygenase (ToMO) is equipped with a broad spectrum of aromatic substrate specificity (such as BTEX; benzene, toluene, ethylbenzene, and isomers of xylenes). TOMO has can hydroxylate more than a single position of aromatic rings in two consecutive monooxygenation reactions. Catechol 1,2-dioxygenase (C1,2D) is an iron-containing...
The co-metabolic biodegradation of p-xylene (as a non-growth substrate model) by Pseudomonas putida (well-known aromatic compounds degrading microorganism) in the presence of Benzene (B), Toluene (T), and Ethylbenzene (E) was studied. Michaelis-Menten/Monod kinetics coupled with co-metabolic degradation expression can be applied for systems contain...
Sites contaminated by petroleum hydrocarbons in cold-climate regions have recently received significant attention due to their sensitive ecosystem and human health impacts. Two cold-adapted pseudomonas strains were isolated from contaminated groundwater and soil. As xylene monooxygenase from Pseudomonas synxantha S2TR-26 and catechol 2,3-dioxygenas...
p-xylene is considered a recalcitrant compound despite the similar aromatic structure with BTE (Benzene, toluene, ethylbenzene). This study evaluated the biodegradation potential of p-xylene by three cold-active Pseudomona s strains (named Pseudomonas putida S2TR-01, Pseudomonas S2TR-20, and Pseudomonas S2TR-09). The catabolic genes ( xylM, xylA an...
It is a general understanding that unconventional oil is petroleum-extracted and processed into petroleum products using unconventional means. The recent growth in the United States (US) shale oil production and the lack of refinery in Canada built for heavy crude processes have resulted in a significant increase in U.S imports of unconventional oi...
Context
Fermented soybean products have been used in various ways, and more research is being conducted on them to reveal their benefit.
Objective
The objective of this study was to evaluate the antioxidative activity of fermented soybean meal extract by Lactobacillus plantarum in vitro and in vivo tests.
Materials and methods
A Lactobacillus pla...
Background: Sepsis is the systemic response of the body to severe infection and one of the causes of mortality in ICU. Considering the importance of sepsis, in the present study, the effect of Non-Steroidal Anti-Inflammatory (NSAIDs) drugs (aspirin, indometacin, celecoxib) on inflammatory biomarker of myeloperoxidase (MPO) in liver and lung tissues...
Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is a ubiquitous transcription factor, which plays a key role in regulating immune response against infection. Increased and/or prolonged activation of NF-κB has been linked to cancer, inflammatory, autoimmune diseases and viral infection. The purpose of this set of data was to e...
Acidithiobacillus sp. obtains energy from the oxidation of Fe 2+ or reduced sulfur compounds. The proteins encoded by the rus operon are involved in the electron transfer from Fe 2+ to O 2. In this research, the expression of rus operon, proposed to evaluate the effects of pulp densities and UV-mutations in the Acidithiobacillus sp. FJ2 in the biol...
Background/aim:
Sepsis is an unregulated systemic response to microbial invasion that can lead to multiple organ failure. This study
aims at investigating the relationships among myeloperoxidase (MPO) and CD177 in major organ systems including whole blood, liver,
and lung tissues in septic rats.
Materials and methods:
Sepsis was induced by cec...
In recent years, there have been advances in bioremediation of pollutants with the goal of effective clean-up of environments in an eco-friendly and cost-effective approach. For contamination removal at cold climate sites, bioremediation approaches are appealing due to their potential to be more cost-effective and efficient than alternative, more e...
In Acidithiobacillus ferrooxidans, one of the most important bioleaching bacterial species, the proteins encoded by the rus operon are involved in the electron transfer from Fe(2+) to O2. To obtain further knowledge about the mechanism(s) involved in the adaptive responses of the bacteria to growth on the different uranium ore pulp densities, we an...
In this study, the effects of Acidithiobacillus sp. FJ2 mutated with diethyl sulfate (DES) and ultraviolet irradiations (UV) on the bioleaching of uranium (U) at high pulp density (50% (wt/vol)) were investigated. The results showed that the oxidative activity of FJ2 is greatly improved by mutation. Correspondingly, the highest leaching rate of ura...
The aim of this study was to determine the effect of low-grade ore pulp density on dissolution of metal by Acidithiobacillus ferrooxidans (A. ferrooxidans). The bioleaching experiments was carried with 9k medium and different pulp densities (2.5,15%) of anomaly II of Saghand in Yazd at 150rpm and 35 o C. The results demonstrated that the variations...
The present study investigated the effects of organic additives on the ability of metal extraction of Acidithiobacillus ferrooxidans (At. f). For this purpose, At. f cultivated with 9k medium and modified 9k medium which supplemented with peptone, tryptic soy broth and yeast extract (organic additives) in the presence of 15% low-grade ores. Then, t...