Saad M Bin Dajem

• Ph.D.
• King Khalid University

Malaria is often characterized by a complicated disease course due to multifaceted intrinsic genetic factors of the host and the parasite. This study aimed to investigate the role of interleukin-27 (IL-27) gene polymorphisms in Plasmodium falciparum malaria infection in a Saudi Arabian cohort. This case-control study obtained blood samples from 250...

Members of the order Trypanorhyncha are cestode parasites that are frequently found infecting the muscles of several marine fish species, affecting fish health, and resulting in consumers’ rejection of fish. Fifty-two specimens of marine fish were freshly caught throughout the year 2020 from boat landing sites at the Alexandria coast along the Medi...

Taking everything into account, the information and attention to diabetes was low, while the pervasiveness of these diseases was high among the number of inhabitants in blood benefactors. This examination discovered that monitoring and having higher diabetes related information can affect the predominance of these contaminations. This is especially...

Drinking water that is secure to drink is a basic human need that should be met by everyone. Waterborne disease prevention and control begin with ensuring the safety of drinking water. The present study was designed to evaluate the bacteriological quality of ground water in twenty different localities of Tehsil Taunsa DG Khan, Punjab Pakistan. For...

Consuming safe drinking water is a simple human want that should be met by way of anyone. Prevention and control of water-borne diseases starts with making sure the protection of drinking water. The present day have a look at turned into designed to assess the physical and chemical stage of groundwater in twenty distinctive areas of Tehsil TaunsaiD...

The thrombospondin related anonymous protein (TRAP) is considered one of the most important pre-erythrocytic vaccine targets. Earlier population genetic studies revealed the TRAP gene to be under strong balancing natural selection. This study is the first attempt to analyze genetic diversity, natural selection, phylogeography and population structu...

Members of the order Trypanorhyncha are cestode parasites that are frequently found infecting the muscles of several marine fish species, affecting fish health and resulting in consumers’ rejection. Seventy–five specimens of marine fish were freshly caught from boat landing sites at the Alexandria coast along the Mediterranean Sea in Egypt, includi...

Background. Malaria is still a public health problem in Saudi Arabia specifically in the Jazan region. Plasmodium falciparum knob-associated histidine-rich proteins (PfKAHRPs) play an important role in cerebral malaria pathophysiology as well as pathogenesis of P. falciparum infections. The repeat region of PfKAHRP C-terminal interaction domain has...

Members of the family Diclidophoridae are potentially dangerous species for the puffer fish aquaculture worldwide. They are parasitic polyopisthocotyleans, with a posterior haptor equipped with clamps for attachment to the host's surface, allowing the worm to resist the flow of water to maintain its position on gills. The anterior body of the worm...

Introduction Interleukin-18 (IL-18) is a pro-inflammatory cytokine, reported to be involved in the initial immune responses against malaria. Genetic variations in the host are an important factor that influences the etiology of malaria at several disease levels. Polymorphisms within the IL-18 gene are associated with susceptibility and clinical out...

During a recent survey on digenean parasites infecting marine fish captured from water located along the Red Sea off Gizan Coasts, Saudi Arabia, thirty-two (29.09%) out of 110 fish were naturally infected with three different species of digenean parasites. The taxonomical status of the recovered worms was confirmed by light microscopy and molecular...

The consumption of inadequately thermally treated fish is a public health risk due to the possible propagation of Anisakis larvae. The present study demonstrated the physiological and histopathological changes that accompanied an oral inoculation of crude extracts from fresh and thermally treated Anisakis Type II (L3) in rats. Worms were isolated f...

The consumption of inadequately thermally treated fish is a public health risk due to the possible propagation of Anisakis larvae and their antigenic proteins, the causative agent of the zoonotic disease anisakidosis. The present study demonstrated the physiological and histopathological changes that accompanied an oral inoculation of crude extract...

Background Bivalves can accumulate and concentrate most pollutants, even if they are present in somewhat low concentrations. The present study aimed to use freshwater bivalveas for the first time as vital indicator for silver/chitosan nanocomposites (Ag-CS NCs) in the freshwater environment. Methods Following the preparation and characterization o...

Despite the progress in using silver nano products in many fields, including medicine, food, and industry, their effects on the environment need more attention. Therefore, the current study aimed to assess the effect of silver/saponin nanocomposites (Ag/S NCs) for the first time on the aquatic environment by using freshwater clam, Caelatura aegypti...

In the present study, pentastomids belonging to the order Cephalobaenida were isolated from the lungs of Berber skinks Eumeces schneideri (Famiy: Scincidae), which were morphologically described by light and scanning electron microscopy and taxonomically justified by 18s rDNA molecular analyses of the parasites. Seventeen host specimens were collec...

To study the genotoxic impacts of Sabal discharges, three sites around Sabal drain were selected and compared to a reference site (site1). Site2 was at the southern part of the main canal, site3 was at the main canal outlet, and site4 at the northern part of the main canal. Compared to the reference fish, the recorded micronuclei and other nuclear...

Metal oxide nanoparticles have wide applications, which have elevated serious alarms about their impacts on the environment. Therefore, we investigated the potential adsorptive capacity of rice husk toward Fe2O3 and Al2O3 nanoparticles to reduce their genotoxic effects. Fish were subjected to 10 mg/l of Fe2O3 and Al2O3 nanoparticles in single and c...

Background and Objectives. Malaria infection, caused by Plasmodium falciparum, is the most lethal and frequently culminates in severe clinical complications. Interleukin-22 (IL-22) has been implicated in several diseases including malaria. The objective of this study was to investigate the role of IL-22 gene polymorphisms in P. falciparum infection...

Introduction Monogenea is a class of ectoparasitic flatworms on the skin, gills, or fins of fish. Microcotylidae is a family of polyopisthocotylean monogeneans parasitising only marine fishes. This work describes and taxonomically determines a microcotylid polyopisthocotylean monogenean in an important fish in Saudi aquaculture. Material and Metho...

Introduction There is still lack of morphological and phylogenetic information on the pathogenic nematode of the camel Haemonchus longistipes . In the present study, this parasite was isolated in Saudi Arabia and described. Material and Methods The abomasa of two Arabian camels were collected from a slaughterhouse in Abha province and examined for...

Schistosomiasis is a fatal disease that has a negative impact on health and economics. Praziquantel (PZQ) is the drug of choice for schistosomiasis treatment, but it has no prophylactic effect; therefore, vaccination is an essential requirement in schistosomiasis control. This work was carried out to investigate the possible effect of DNA vaccinati...

This report introduced the description of two different species of digenean parasites isolated from the intestine of Rhinopoma hardwickii with new host and locality records in Egypt. The recovered helminthes were studied morphologically and morphometrically by light microscopy and the surface topography of the two species was elucidated by scanning...

Natural products, including their purified materials, play a remarkable role in drug development. The Euphorbiaceae family, mainly Euphorbia tirucalli, is used in some traditional medicine, and has evidence that its latex comprises immunomodulatory properties and cytokine production. This study aimed to measure the in vivo production of chemokines...

Fresh muscle samples from water buffalo (Bubalus bubalis) aged 2–15, from Giza Province, Egypt; were examined for Sarcocystis infection. Macroscopic ovoid sarcocysts embedded in the muscle tissues of the examined buffaloes were detected; they measured 152–230 (210 ± 7) µm in length and 37–119 (95 ± 3) µm in width. The esophagus was the most infecte...

The profound impact of Information Technology on data, in general, has gone to the extent that incredible possibilities are open for data handling in spite of alarming data characteristics. The nature of cybercrime and constant evolvement of the field has expanded over the last few decades, and it is now on a climatic rise. As a result, research of...

The apical membrane antigen 1 of Plasmodium falciparum (PfAMA1) plays a crucial role in erythrocyte invasion and is a target of protective antibodies. Although domain I of PfAMA1 has been considered a promising vaccine component, extensive sequence diversity in this domain could compromise an effective vaccine design. To explore the extent of seque...

The genetic diversity of Plasmodium falciparum infections in human is associated with the pathogenesis of malaria. It is commonly determined through amplification of the polymorphic regions of the merozoite surface proteins -1 (msp-1) and -2 (msp-2) genes. This study aimed to (1) determine the prevalence of the msp-1 and msp-2 allelic familiesand (...

To investigate the incidence of mixed-species (MS) malaria infection, and compare the results with microscopically confirmed cases of malaria. During 2010, blood spots collected from 371 clinically suspected cases of malaria were microscopically examined in a cross-sectional study. The DNA was extracted from the samples, and a nested polymerase cha...

Background: Transfusion-transmissible infectious agents such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV) and syphilis are among the greatest threats to blood safety for the recipient. Aim: This study aimed at determining the seroprevalence of transmissible bacteria such as syphilis and viruses as HIV, HB...

Background: Rapid diagnostic tests for malaria are now a commonly used procedure for malaria diagnosis. Despite some problems related to sensitivity and applicability, malaria rapid diagnostic tests (RDTs), are currently considered the best option to overcome well trained experts in blood banks and fast releas of blood units. Objectives: To detect...

Schistosoma mansoni is mediated through the intermediate host Biomphalaria arabica which lives in Saudi Arabia. Molecular characterization and identification of this intermediate host are important for epidemiological studies of schistosomiasis. The present work aimed to determine the molecular variations among the populations of B. arabica found i...

This study aimed to determine the seroprevalence of Toxoplasma gondii in pregnant women in the south western region of Saudi Arabia and to find out the possible risk factors that may lead to infection. This cross sectional hospital based study was carried out at three hospitals in the south western region of Saudi Arabia from January 2008 to August...

Babesia spp. are malaria-like protozoans that parasitize and reproduce within mammalian red blood cells. These parasites have medical, veterinary and economic importance, since they caused significant losses in livestock and serious health problems to human. In spite of the great importance of these parasites, little information is available about...

Primary toxoplasma infection during pregnancy carries a risk of fetal damage. The most frequent challenge encountered is how to determine if a pregnant woman acquired the acute infection during gestation. This study aimed to determine the incidence of acute maternal toxoplasmosis by a reliable method and to estimate the rate of intrauterine transmi...

A major challenge to the success of malaria control program in Saudi Arabia is the high influx of expatriates and holy visitors from malaria endemic countries. In the present study we examined whether drug resistant parasite genotypes reported in Jazan region, southwest of Saudi Arabia are imported or developed locally. We examined 178 Plasmodium f...

Toxoplasmosis, caused by Toxoplasma gondii, is diagnosed mainly by serological methods that are hindered by insufficient sensitivity. When it fails, it becomes necessary to rely on either direct detection of the parasite or DNA detection by polymerase chain reaction (PCR). We aimed to establish molecular tools for toxoplasmosis research in the coun...

Two hundred and three Plasmodium falciparum isolates from Jazan area, southwest Saudi Arabia, were typed for Pfcrt, Pfmdr1, dhps, and dhfr mutations associated with resistance to chloroquine, mefloquine, halofantrine, artemisinin, sulfadoxine-pyrimethamine, and the neutral polymorphic gene Pfg377. A large proportion (33%) of isolates harbored doubl...

Varicella-zoster virus (VZV) is the etiologic agent of two diseases, varicella (chicken pox) and zoster (shingles). Varicella is a self- limited infection, while zoster is mainly a disease of adults. The present study was conducted to isolate VZV from clinically diagnosed children using cell cultures and compare the activity of liquorice powder ext...

This work aimed to determine the inter- and intra-specific variations in populations of Bulinus truncatus and Bulinus beccari, the intermediate hosts of Schistosoma haematobium in Saudi Arabia, and to develop species-specific primers to identify these snails as a first step in the development of multiplex PCR for simultaneously identifying the snai...

This study was conducted to assess the effect of ginger (Zingiber officinale) aqueous extract, on the oxidative status, antioxidant defense system and liver pathology of Schistosoma mansoni -infected C57BL/6 mice. Ginger at dose level of 500 mg/kg body weight was orally administered, daily for five weeks from the 5 th week post-infection. Result sh...

This study was designed to assess the effect of green tea, an aqueous extract of Camellia sinensis, on the oxidative stress, antioxidant defense system and liver pathology of Schistosoma mansoni-infected mice. Green tea at concentration of 3% (w/v) was given orally to treated mice as sole source of drinking water from the end of the 4th week to the...

In the time schistosomisis control programs are implemented in many countries, schistosomiasis continues to spread throughout the world. Among these control strategies is the vector control. Within this context, analysis of the genetic variability of the intermediate host snails is important because it allows identification of specific sequences of...

CKGROUND: There have been recent reports on the successful use of recombinant factor VIIa (rFVIIa) in non-hemophiliac patients who have experienced heavy blood loss due to trauma with extensive organ damage and who have received multiple blood transfusions with hemostatic changes without success. The timing of administration, dosage, mortality, uni...

The emergence of chloroquine resistance in Plasmodium falciparum is a significant public health problem where malaria is endemic. We aimed to evaluate the efficacy of pyrosequencing to assess chloroquine resistance among P. falciparum isolates from the southwestern region of Saudi Arabia by analyzing the K76T and N86Y mutations in the P. falciparum...

Several expeditions were carried out to four localities (Al-Madinah Almona-warah, Tabouk region, Al-Jouf and Northern Frontiers regions) in Northern and Western Saudi Arabia for sampling zoonotic cutaneous leishmaniasis (ZCL) cases from patients and rodents. Biopsy samples were collected from 51 patients complaining of skin lesions, most of which (...

Many regions of the world, including Saudi Arabia, are medically vulnerable by the spread of leishmaniasis. Although the effectiveness, the current available drugs suffer from the appearance of resistant strains in epidemic areas and their toxic side-effects. We report the use of the iron chelator deferoxamine (DFRX) that can be used as antileishma...

The alteration in the concentrations of metallic ion Pb, Zn, K, Na, Co, Fe, and Cu in the soft parts of the Biomphalaria alexandrina snails shedding Schistosoma mansoni cercariae was detected by flame atomic absorption spectrometry. Six elements Pb, Zn, K, Na, Co, and Cu were found to be present at significantly higher concentrations in cercariae-s...

Chloroquine has been the drug of choice for the treatment of malaria for many decades. We aimed to examine the molecular basis of chloroquine resistance among Plasmodium falciparum isolates from the southwestern region of Saudi Arabia by analyzing the K76T and N86Y mutations in the PfCRT and PfMDR1 genes, respectively. P falciparum-infected blood s...

The present study gave information about the recent distribution of freshwater snails in Asser region, and the current status of trematode infection specially schistosomiasis within the snails. Fifteen localities were visited from Septeinber 2007 to December 2008 and the collected snails were examined for the presence of trematode infection. Seven...

A laboratory-based susceptibility study was carried out on snails Bulinus truncatus collected from highland Abha, Asser, Saudi Arabia to Egyptian Schistosoma haematobium to investigate the potential role of Saudi B. truncatus in the transmission of Egyptian S. haematobium and to know the possibility that the parasite might be able to spread into Sa...

To document the distribution of the ABO and rhesus (Rh) blood groups in a random sample of Saudi students from the King Khalid University, Abha, Kingdom of Saudi Arabia, and to compare our results from that of other studies in the Kingdom and elsewhere. The subjects included in this study were 944 males from the southwest region of Saudi Arabia inc...

In this article, two strains of mice BALB/C and C57 were infected with Egyptian strain of Schistosoma mansoni. BALB/C mice appeared to harbor fewer parasites than did C57 mice. The hepatic and intestinal tissues of C57 mice were loaded with more eggs than that of BALB/C mice. Regardless the strain of mice, the number of eggs per gram of liver tissu...

To detect the prevalence of urinary schistosomiasis and Bulinus truncatus snail, 150 urine samples and 837 B. truncatus snails were collected from Qena Governorate in Upper Egypt. Infection rates were 15.7% (age-period 8-17 years) among males and 20.7% among females (age-period 9-14 years). The highest collected snails number was found in January a...

In the present work, scanning electron microscopical studies on Schistosoma mansoni cercariae exposed to ultraviolet irradiation (UV-irradiation) were done to answer the question: Is the damage inflicted seen on the adult schistosome worms developed from irradiated cercariae, due to direct effect of UV-irradiation on cercariae or due to the host's...