s. M. Rafiqul Islam

s. M. Rafiqul Islam
University of Chittagong · Department of Genetic Engineering and Biotechnology

PhD; Post Doc (Bioprocess Engineering)

About

38
Publications
31,221
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550
Citations
Introduction
Dr. S. M. Rafiqul Islam is currently working as a Professor in the Department of Genetic Engineering and Biotechnology, University of Chittagong, Bangladesh. Professor Rafiqul has recently completed PhD and Postdoc in Bioprocess Engineering at University Malaysia Pahang.
Education
August 2009 - October 2012
Universiti Malaysia Pahang, Kuantan, Malaysia
Field of study
  • Bioprocess Engineering

Publications

Publications (38)
Article
Full-text available
The emergence of multidrug-resistant Klebsiella pneumoniae (Kpn) is a global concern due to the increasing rate of mortality and hospital cost burden in the affected population. This study reports the whole-genome sequences of nine multidrug-resistant Kpn from a hospital in Chattogram city of Bangladesh.
Article
Full-text available
The white spot syndrome virus (WSSV) is a causative agent of white spot disease (WSD) in crustaceans, especially in cultivated black tiger shrimp (Penaeus monodon), leading to significant economic losses in the aquaculture sector. The present study describes four whole genome sequences of WSSV obtained from coastal regions of Bangladesh.
Article
Full-text available
Background The coronavirus disease 2019 (COVID‐19) pandemic has had a severe impact on population health. The genetic determinants of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in southern Bangladesh are not well understood. Methods This study aimed to determine the genomic variation in SARS‐CoV‐2 genomes that have evolved over 2...
Article
Full-text available
Background The host-microbe interactions are complex, dynamic and context-dependent. In this regard, migratory fish species like hilsa shad (Tenualosa ilisha), which migrates from seawater to freshwater for spawning, provides a unique system for investigating the microbiome under an additional change in fish’s habitat. This work was undertaken to d...
Preprint
Full-text available
Background: Stearoyl-CoA desaturase 1 (SCD1) activity, a key lipid metabolism regulator, may impact type 2 diabetes (T2D). Previously, we reported a significant functional interaction between SCD1 and T2D in the Bangladeshi population. In the present study, we investigated the relationship between the SCD1 rs7849 polymorphism and T2D risk variables...
Article
Full-text available
Populations of different South Asian nations including Bangladesh reportedly have a high risk of developing diabetes in recent years. This study aimed to investigate the differences in the gut microbiome of COVID-19 positive participants with or without T2DM compared with healthy control subjects. Microbiome data of thirty participants with T2DM we...
Article
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Yogurt is an ethnic fermented food that dominates as an integral part of human diet for its health benefits. The nutritional quality of yogurt is diminished due to the presence of adulterants, metal and microbial contaminants. Upon consumption, these materials cause metabolic disorders and food-borne diseases. This study was designed to explore the...
Article
Full-text available
Coronavirus disease-2019 (COVID-19) is an infectious disease caused by SARS-CoV-2 virus. The microbes inhabiting the oral cavity and gut might play crucial roles in maintaining a favorable gut environment, and their relationship with SARS-CoV-2 infection susceptibility and severity is yet to be fully explored. This study investigates the diversity...
Article
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Yogurt is one of the most frequently consumed dairy products for nutritional benefits. Although yogurt is enriched with probiotics, it is susceptible to spoilage because of the presence of pathogenic microbes. Spoiled yogurt if consumed can cause food-borne diseases. This study aimed to assess the nutritional composition and microbiome diversity in...
Preprint
Full-text available
Yogurt quality mainly depends on nutritional properties, microbial diversity and purity of starter culture. This study aimed to assess the nutritional composition and microbiome diversity in yogurt. Microbial diversity was analyzed by 16S and 18S rRNA based high-throughput sequencing. Significantly (P<0.05) higher pH, fat, moisture, total solid and...
Article
Enzymatic production of bioxylitol from lignocellulosic biomass (LCB) provides a promising alternative to both chemical and fermentative routes. This study aimed to assess the impacts of catalytic variables on bioxylitol production from wood sawdust using xylose reductase (XR) enzyme and to optimize the bioprocess. Enzyme-based xylitol production w...
Chapter
Hemicellulose, a heteropolysaccharide, is a second major component of lignocellulosic biomass (LCB). It is a potential source of various rare sugars, mainly xylose, because the biomass is cheap, renewable, and available globally. Xylose can be an economic and attractive substrate to produce numerous specialty chemicals, especially xylitol. It is pa...
Article
Full-text available
Xylitol purification is the most difficult step of the whole manufacturing process and its purification being of commercial importance. This study aimed to purify bioxylitol by liquid-liquid extraction (LLE) from enzymatic reaction mixture. Bioxylitol extraction was conducted under various conditions of extraction time (45–105 min) sample to solven...
Article
Full-text available
Xylose-rich sawdust hydrolysate can be an economic substrate for the enzymatic production of xylitol, a specialty product. It is important to identify the process factors influencing xylitol production. This research aimed to screen the parameters significantly affecting bioxylitol synthesis from wood sawdust by xylose reductase (XR). Enzymatic bio...
Article
Full-text available
Sawdust hemicellulosic hydrolysate can be used as a promising xylose source for enzymatic conversion to xylitol, a specialty product. The aim of this study was to design various factors by observing their effects on xylitol production from hemicellulosic hydrolysate by xylose reductase (XR) enzyme. The one-factor-at-a-time (OFAT) strategy was adopt...
Article
Full-text available
Xylose reductase (XR) is an intracellular enzyme, which catalyzes xylose to xylitol conversion in the microbes. It has potential biotechnological applications in the manufacture of various commercially important specialty bioproducts including xylitol. This study aimed to prepare XR from adapted strain of Candida tropicalis and to characterize it....
Article
Full-text available
Xylose reductase (XR) is an oxidoreductase having potential applications in the production of various specialty products, mainly xylitol. It is important to screen for compounds that can decrease XR activity and consequently can decrease xylitol production. We have identified the byproducts in the hemicellulosic hydrolysate that inhibit XR from Can...
Article
Full-text available
Xylitol production by bioconversion of xylose can be economically interesting if the raw material can be recovered from a cheap lignocellulosic biomass (LCB). Meranti wood sawdust (MWS) is a renewable and low-cost LCB that can be used as a promising and economic source of xylose, a starting raw material for the manufacture of several specialty chem...
Article
Xylose, recovered from Meranti wood sawdust (MWS), can be used as a promising and alternative carbon source for yeast growth as well as for the production of xylose reductase (XR). This enzyme has potential applications in the bioproduction of various high value products, especially xylitol. The aim of this study was to isolate XR from adapted yeas...
Article
Full-text available
Staphylococcus aureus is a gram positive bacterium, responsible for both community-acquired and hospital-acquired infection, resulting in a mortality rate of 39%. 43.2% resistance to methicilin and emerging resistance to Fluroquinolone and Oxazolidinone, have evoked the necessity of the establishment of alternative and effective therapeutic approac...
Article
Full-text available
Meranti wood sawdust (MWS) is a cheap and widely available lignocellulosic biomass, which can be a potential source of xylose. This xylose can be an economic raw material for the production of a wide variety of specialty chemicals, mainly xylitol. It is particularly important to establish rapid hydrolysis conditions, which can yield xylose-rich hyd...
Article
Full-text available
Xylitol is a high value sugar alcohol with anticariogenic properties that is used as an ideal sweetener for diabetic patients. Industrially, xylitol is manufactured by catalytic reduction of pure xylose, which has some disadvantages. The fermentation process has been studied as an alternative, but its viability is dependent on the optimization of s...
Article
Full-text available
Xylitol is a sugar alcohol that is used as a sweetener for diabetics and also for other purposes (e.g., in chewing gum). Industrially, xylitol is manufactured through a chemical process that has some disadvantages, including a high energy requirement, extensive purification steps, and a high cost of product. The microbial production of xylitol has...
Article
Full-text available
Meranti wood sawdust (MWS) is a lignocellulosic waste of sawmill which can be used as a promising source of xylose. The main application of xylose is its bioconversion to xylitol, a high value product. The growing interest in biotechnological production of biofuels and specialty chemicals from lignocellulosic waste is justifiable as these materials...
Article
Full-text available
A newly synthesized Nickel (II) tyrosine complex was screened as potential antimicrobial agent against a number of medically important bacteria (Bacillus subtilis, Streptococcus β-haemolytica, Escherichia coli, Shigella dysenterae) and fungi (Aspergillus fumigatus, Candida albicans, Aspergillus niger, Aspergillus flavus, Penicillium sp.) strains. w...
Article
The xylanase-producing bacterial strains were screened from damp straw. The strains were isolated on xylan agar media and screening was carried out by xylanolysis method. The xylanase-producing capacity of the isolates was further confirmed by DNS (dinitrosalisylic acid) method. The isolated strains were identified as Aeromonas on the basis of morp...
Article
Three electrophoretically homogeneous lectins were purified from the extract of mulberry seeds. The two lectins i.e. MSL-2 and MLS-3 were purified by gel filtration of 100% ammonium sulfate saturated crude extract followed by ion-exchange chromatography on DEAE-cellulose while another MSL-1 was purified by further chromatography on CM-cellulose col...
Article
Full-text available
Aeromonas strain is one of the predominant agents producing xylanase enzymes that are used for degrading the xylosidic linkages of xylan backbone in plant kingdom. Aeromonas strains were isolated from damp soil and straw at Rajshahi region. The strains were isolated on xylan agar media and screened by β-xylanolysis method. The isolated xylanase-pro...

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