Ryan W Bogard

Harvard Medical School | HMS · Department of Microbiology and Immunobiology

Unlabelled: LysR-type transcriptional regulators (LTTRs) are the largest, most diverse family of prokaryotic transcription factors, with regulatory roles spanning metabolism, cell growth and division, and pathogenesis. Using a sequence-defined transposon mutant library, we screened a panel of V. cholerae El Tor mutants to identify LTTRs required f...

The function of the Vibrio 7(th) pandemic island-1 (VSP-1) in cholera pathogenesis has remained obscure. Utilizing chromatin immunoprecipitation sequencing and RNA sequencing to map the regulon of the master virulence regulator ToxT, we identify a TCP island-encoded small RNA that reduces the expression of a previously unrecognized VSP-1-encoded tr...

ChIP coupled with next-generation sequencing (ChIP-seq) has revolutionized whole-genome mapping of DNA-binding protein sites. Although ChIP-seq rapidly gained support in eukaryotic systems, it remains underused in the mapping of bacterial transcriptional regulator-binding sites. Using the virulence-required iron-responsive ferric uptake regulator (...

A. Growth of wild type (black square), ΔmutS (red triangle), Δnfo (blue circle) and xth::Tn Δnfo (green diamond) in LB at pH 7.0. Growth was measured by changes in the cultures OD600 readings. The averages of 3 experiments are shown for each strain. B. Complementation of the mutator phenotype of the ΔmutS mutant. The number of wild type+pBAD18 colo...

Exponentially growing cultures of wild type and ΔmutS, Δnfo, ΔprxA, ΔhmpA and Δnfo xth::Tn mutants were grown in LB buffered at pH 7.0 in the absence (A) or presence (B) of 400 µM sodium nitrite. Growth was measured by changes in the cultures OD600 readings. The average of three experiments is shown for each strain. Wild type (black squares), ΔmutS...

Primers used in this study. (0.03 MB DOC)

ID numbers/ Accession numbers for genes used in this study. (0.03 MB DOC)

A. Hydrogen peroxide sensitivity. Wild type (•) and ΔprxA mutant (▪) were plated on agar containing increasing concentration of the hydrogen peroxide (H2O2). Cfu were determined after overnight growth. The average of 3 experiments is shown. (0.43 MB EPS)

Colonization of C57B and isogenic iNOS−/− infant mice by the hmp::Tn mutant. (0.02 MB DOC)

Ability of V. cholerae mutants defective in DNA repair pathways to colonize the infant mouse intestine in competition with the parental strain (WT) 3 h post inoculation. (0.03 MB DOC)

Bacterial strains and plasmids used in this study. (0.06 MB DOC)

Ingested Vibrio cholerae pass through the stomach and colonize the small intestines of its host. Here, we show that V. cholerae requires at least two types of DNA repair systems to efficiently compete for colonization of the infant mouse intestine. These results show that V. cholerae experiences increased DNA damage in the murine gastrointestinal t...

We recently reported a simple PCR procedure that targets a sequence variation of the virulence-correlated gene locus vcg. It was found that 90% of all clinical isolates possessed the vcgC sequence variant, while 93% of all environmental isolates possessed the vcgE sequence variant. Here we report that the clinical genotype of Vibrio vulnificus is s...