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Ruvini Kurukulasooriya

Ruvini Kurukulasooriya
Ruhuna Duke Centre for Infectious Diseases (RDCID) Faculty of Medicine Univerity of Ruhuna

BSc., MSc., PhD (Reading)
Reading a PhD

About

41
Publications
2,865
Reads
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174
Citations
Citations since 2017
31 Research Items
171 Citations
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Introduction
A science graduate holding a BSc honours degree in Agriculture (University of Ruhuna, Sri Lanka) and a MSc in Agric. Biology (University of Peradeniya, Sri Lanka). Research experience: Biodiversity, molecular epidemiology of plants and plant diseases, and human infectious disease diagnosis. Field of interest: Molecular Biology, Biotechnology and Microbiology. Currently reading for a PhD in Molecular biology and Microbiology.
Additional affiliations
April 2010 - March 2011
Durdans Hospital
Position
  • Junior Molecular Biologist
Description
  • Molecular diagnostic of diseases (Microbacterium Tuberculosis, Dengue, Hepatitis B & C, Herpes Simplex, HIV, Chikungunya) by performing PCR and RT-PCR assays.
August 2009 - March 2010
Agricultural Biotechnology Center, University of Peradeniya
Position
  • Research Associate
Description
  • Conducted Biotechnology related demonstrations and practical classes to Undergraduate, Postgraduate and Open university students, organized and conducted workshops and contributed to the research activities of the Institute.
August 2007 - December 2008
Plant Genetic Resources Centre, Ministry of Agriculture
Position
  • Research Assistant
Description
  • Molecular detection of resistant genes for Bacterial Leaf Blight disease of rice under the project; “Pyramiding of genes for resistance to Bacterial Leaf Blight into new improved rice varieties”.
Education
July 2016 - July 2022
Faculty of Medicine, University of Ruhuna
Field of study
  • Microbiology and Molecular biology
October 2007 - October 2009
University of Peradeinya
Field of study
  • Plant Molecular Biology
April 2003 - June 2007
University of Ruhuna
Field of study
  • Agriculture

Publications

Publications (41)
Preprint
Full-text available
Limited diagnostics challenge management of acute febrile illness and sepsis (AFI/sepsis) globally. We generated transcriptomes for a 294-participant (USA, Sri Lanka) discovery cohort with AFI/sepsis. We used lasso to derive gene expression classifiers followed by cross-validation and generated: 1) a single model to distinguish bacterial vs. viral...
Article
Full-text available
Background The Southern Province of Sri Lanka is endemic with dengue, with frequent outbreaks and occurrence of severe disease. However, the economic burden of dengue is poorly quantified. Therefore, we conducted a cost analysis to assess the direct and indirect costs associated with hospitalized patients with dengue to households and to the public...
Article
Full-text available
Background To develop effective antimicrobial stewardship programs (ASPs) for low- and middle-income countries (LMICs), it is important to identify key targets for improving antimicrobial use. We sought to systematically describe the prevalence and patterns of antimicrobial use in three LMIC hospitals. Methods Consecutive patients admitted to the...
Article
Full-text available
Background Healthcare systems in dengue-endemic countries are often overburdened due to the high number of patients hospitalized according to dengue management guidelines. We systematically evaluated clinical outcomes in a large cohort of patients hospitalized with acute dengue to support triaging of patients to ambulatory versus inpatient manageme...
Article
Full-text available
Viruses cause a wide spectrum of clinical disease, the majority being acute respiratory infections (ARI). In most cases, ARI symptoms are similar for different viruses although severity can be variable. The objective of this study was to understand the shared and unique elements of the host transcriptional response to different viral pathogens. We...
Article
Antibiotic resistance is an emerging global public health threat. One of the main drivers of this threat is the inappropriate use of antibiotics. In Sri Lanka, antibiotic consumption is increasing, but little is known locally about how patients perceive antibiotics. We conducted a qualitative study to gain a better understanding of the knowledge, p...
Article
Full-text available
Background The timing of and risk factors for intestinal colonization with multidrug-resistant Enterobacteriaceae (MDRE) are still poorly understood in areas with high MDRE carriage. We determined the prevalence, timing, and risk factors associated with MDRE intestinal colonization among infants in southern Sri Lanka. Methods Women and their newbo...
Article
Full-text available
Background Inappropriate antibiotic use is linked to the spread of antimicrobial resistance worldwide, but there are limited systemic data on antibiotic utilization in low- and middle-income countries. The purpose of this study was to evaluate the prevalence and patterns of antibiotic prescription in an ambulatory care setting in Sri Lanka. Method...
Poster
Full-text available
Background: Lower respiratory tract infection (LRTI) is the most common infectious cause of death worldwide. The most common bacterial agent for LRTI is Streptococcus pneumoniae. The conjugate pneumococcal vaccine protects against S. pneumoniae but is not available through the public healthcare sector of Sri Lanka. Data regarding LRTI burden d...
Conference Paper
Full-text available
Lower respiratory tract infection (LRTI) is the most common infectious cause of death worldwide. The most common bacterial agent for LRTI is Streptococcus pneumoniae. The conjugate pneumococcal vaccine protects against S. pneumoniae but is not available through the public healthcare sector of Sri Lanka. Data regarding LRTI burden due to S. pneumoni...
Article
Full-text available
Abstract Objectives: To determine aetiology of illness among children and adults presenting during outbreak of severe respiratory illness in Southern Province, Sri Lanka, in 2018. Design: Prospective, cross-sectional study. Setting: 1600-bed, public, tertiary care hospital in Southern Province, Sri Lanka. Participants: 410 consecutive patients,...
Article
Full-text available
Background Pathogen-based diagnostics for acute respiratory infection (ARI) have limited ability to detect etiology of illness. We previously showed that peripheral blood-based host gene expression classifiers accurately identify bacterial and viral ARI in cohorts of European and African descent. We determined classifier performance in a South Asia...
Article
Full-text available
Background: Dengue is a major cause of acute febrile illness in Sri Lanka. Dengue has historically been considered an urban disease. In 2012-2013, we documented that acute dengue was surprisingly associated with self-reported rural residence in the Southern Province of Sri Lanka. Methods: Patients admitted with an acute febrile illness were enro...
Article
Full-text available
Background Skin and soft-tissue infections (SSTI) are a common reason for antimicrobial use in the outpatient and inpatient settings. Inappropriate antimicrobial use for SSTI is common. We determined the prevalence of SSTI and associated inappropriate antimicrobial use among inpatients in Sri Lanka. Methods A point-prevalence study of antimicrobia...
Article
Full-text available
Background Acute respiratory infections are a common reason for antibiotic overuse. We previously showed that providing Sri Lankan clinicians with positive rapid influenza test results was associated with a reduction in antibiotic prescriptions. The economic impact of influenza diagnostic strategies is unknown. Methods We estimated the incremental...
Article
The contribution of respiratory viruses to acute febrile illness (AFI) burden is poorly characterized. We describe the prevalence, seasonality, and clinical features of respiratory viral infection among AFI admissions in Sri Lanka. We enrolled AFI patients ≥ 1 year of age admitted to a tertiary care hospital in southern Sri Lanka, June 2012-October...
Article
Full-text available
A point-prevalence study of antimicrobial use among inpatients at 5 public hospitals in Sri Lanka revealed that 54.6% were receiving antimicrobials: 43.1% in medical wards, 68.0% in surgical wards, and 97.6% in intensive care wards. Amoxicillin-clavulanate was most commonly used for major indications. Among patients receiving antimicrobials, 31.0%...
Article
Full-text available
Background Acute febrile illness is a frequent cause of hospitalization in the tropics and often presents with respiratory symptoms, even when caused by nonrespiratory pathogens. Previously, host-based gene expression signatures accurately identified acute respiratory infections as being bacterial or viral in a U.S. cohort. We determined signature...
Article
Full-text available
Background Dengue is a leading cause of fever and mimics other acute febrile illnesses (AFI). In 2009, the World Health Organization (WHO) revised criteria for clinical diagnosis of dengue. Methodology/Principal findings The new WHO 2009 classification of dengue divides suspected cases into three categories: dengue without warning signs, dengue wi...
Conference Paper
Full-text available
Assess the diagnostic efficacy of Leptospira IgM ELISA"Serion-viron" and IgM Immunochromatography test "LeptocheckWB" in the diagnosis of leptospirosis during the acute stage of the illness. ....
Conference Paper
Full-text available
Molecular and antibiotic profile confirmed that more than half of patients were colonised with CA-MRSA on admission .......
Conference Paper
Full-text available
Methicillin-resistant Staphylococcus aureus (MRSA) causes a substantial burden of infection. Colonization with MRSA is a recognized risk factor for infection. The study describes prevalence and in-hospital acquisition of MRSA colonization.
Article
Full-text available
In tropical and subtropical settings, the epidemiology of viral acute respiratory tract infections varies widely between countries. We determined the etiology, seasonality, and clinical presentation of viral acute respiratory tract infections among outpatients in southern Sri Lanka. From March 2013 to January 2015, we enrolled outpatients presentin...
Article
Full-text available
Background Dengue is a frequent cause of acute febrile illness with an expanding global distribution. Since the 1960s, dengue in Sri Lanka has been documented primarily along the heavily urbanized western coast with periodic shifting of serotypes. Outbreaks from 2005–2008 were attributed to a new clade of DENV-3 and more recently to a newly introdu...
Article
Full-text available
Acute respiratory tract infections (ARTIs) are a common reason for unnecessary antibiotic prescriptions worldwide. Our objective was to determine if providing access to rapid influenza test results could reduce antibiotic prescriptions for ARTIs in a resource-limited setting. We conducted a prospective, pre-post study from March 2013 to October 201...
Article
Full-text available
Influenza accounts for a large burden of acute respiratory tract infections in high-income countries; data from lower-income settings are limited due to lack of confirmatory testing. Consecutive outpatients presenting to the largest tertiary care hospital in southern Sri Lanka were surveyed for influenza-like illness (ILI), defined as acute onset o...
Conference Paper
Background: Acute respiratory illnesses, including influenza, account for a large proportion of ambulatory care visits worldwide. In the developed world, these encounters commonly result in unwarranted antibiotic prescriptions; data from more resource-limited settings are lacking. Methods: Consecutive patients presenting to the Outpatient Dep...
Article
Banana is an important fruit crop in the world. Genetic variability present in Musa spp. in Sri Lanka is rich and needs characterization for its conservation and use. Genetic characterization using SSRs is a rapid and accurate technique complementary to morphological characterization. In this research, 27 Musa germplasm (M. acuminata, M. balbisiana...
Article
Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. Oryzae, is one of the most destructive diseases of rice in Sri Lanka. Cultivation of varieties having multiple resistant genes will minimize the pesticide use while avoiding the risk of disease epidemics. Pyramiding of genes by Molecular Marker Assisted Selection (MAS) has proven to be th...
Conference Paper
Musa balbisiana has been used as a parent in several cultivars due to its disease resistance, drought tolerance and general hardiness to the environmental conditions. This study was conducted at Plant Genetic Resources Centre with the objective of the characterization of the genetic resources of wild Musa. Samples were collected from previously rec...

Questions

Question (1)
Question
I have received a particular ST type for an isolate after analyzing F/R sequences received for seven housekeeping genes of Staph.
This is the process I followed.
1. Took Upstream sequence
2. Took Downstream sequence
3. Got the reverse complement sequence of Downstream
4. Aligned them on Bioedit
5. Made the consensus sequences
7. Repeat it for another three (out of seven) sequences and the most compatible MLST profile was selected using
Is there a different way of doing this or any other method of producing promising results?
Thanks a lot for your suggestions in advance.

Network

Cited By

Projects

Project (1)
Project
To identify the detailed data on the proportion of colonization of the bacteria, it's spreading, potential risk factors to cause the diseases, resistance to antibiotics, relevant genotypic information and the influence of natural bacteria over the MRSA which are associated with hospitals, communities and other environments (animal and animal products) in Southern Province of Sri Lanka under a One-Health approach.