Rui Oliveira-Silva

Rui Oliveira-Silva
Technical University of Lisbon | UTL · Institute for Biotechnology and Bioengineering (IBB)

PhD student in Biotechnology and Bioengineering

About

16
Publications
4,061
Reads
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229
Citations
Citations since 2017
9 Research Items
206 Citations
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2017201820192020202120222023010203040
2017201820192020202120222023010203040
2017201820192020202120222023010203040
Additional affiliations
May 2019 - present
Eindhoven University of Technology
Position
  • PhD Student
Description
  • I am enrolled in the MBx group under the supervision of Doctor Peter Zijlstra
September 2016 - April 2019
Technical University of Lisbon
Position
  • PhD Student
September 2013 - August 2016
University of Aveiro
Position
  • Research Assistant

Publications

Publications (16)
Article
Proteases play a pivotal role in several biological processes, from digestion, cell proliferation, and differentiation to fertility. Deregulation of protease metabolism can result in several pathological conditions (i.e., cancer, neurodegenerative disorders, and others). Therefore, monitoring proteolytic activity in real time could have a fundament...
Article
Full-text available
A simple method based on sucrose density gradient centrifugation is proposed here for the fractionation of colloidal silver nanotriangles. This method afforded particle fractions with surface plasmon resonances, spanning from red to infrared spectral ranges that could be used to tune optical properties for plasmonic applications. This feature was e...
Article
Full-text available
The search for sustainable catalytic oxidation processes remains a challenge. One process of utmost industrial and economic importance is the selective oxidation of cyclohexane, in the route of nylon-6,6 production, which requires urgent improvement. Herein, Au nanotriangles (Au NTs) were prepared following a three-step (seed preparation, growth an...
Article
While a continuous monitoring of temperature at the micro and nano-scales is clearly of interest in many contexts, in many others a yes or no answer to the question "did the system locally exceeded a certain temperature threshold?" can be more accurate and useful. This is the case of hard-to-detect events, such as those where temperature fluctuatio...
Article
Full-text available
This paper reports about a study of the local plasmonic resonance (LSPR) produced by metal nanoparticles embedded in a dielectric or semiconductor matrix. It is presented an analysis of the LSPR for different nanoparticle metals, shapes, and embedding media composition. Metals of interest for nanoparticle composition are Aluminum and Gold. Shapes o...
Article
Full-text available
The development of portable low-cost integrated optics-based biosensors for photonics-on-a-chip devices for real-time diagnosis are of great interest, offering significant advantages over current analytical methods. We report the fabrication and characterization of an optical sensor based on a Mach-Zehnder interferometer to monitor the growing conc...
Poster
Full-text available
Introduction Chronic periodontitis (CP) is a complex immuno-inflammatory disease affecting the periodontium, the tissue that give support to the teeth. Its severity is associated to the destruction of the periodontal ligament, deepening of periodontal pockets, alveolar bone resorption and even tooth loss. So far, the diagnosis of CP relies on subje...
Article
The widespread use of silver nanoparticles (AgNPs) is accompanied by a growing concern regarding their potential risks to human health, thus calling for an increased understanding of their biological effects. The aim of this work was to systematically study the extent to which changes in cellular metabolism were dependent on the properties of AgNPs...
Article
The efforts dedicated to improving water decontamination procedures have prompted the interest in the development of efficient, inexpensive, and reusable sorbents for the uptake of dye pollutants. In this work, novel sorbents consisting of carrageenan polysaccharides grafted to magnetic iron oxide nanoparticles were prepared. κ- and ι-carrageenan w...
Article
Chronic periodontitis (CP) is a complex immuno-inflammatory disease that results from pre-established gingivitis. We investigated potential differences in salivary peptidome in health and CP. Saliva was collected from 9 CP patients and 10 healthy subjects, from which 5 CP and 5 healthy were enriched following endoProteoFASP approach, separated and...
Article
Protein post-translational modifications (PTMs) have increasingly become a research field of incredible importance to fully understand the regulation of biological processes in health and disease. Among PTMs, glycosylation is one of the most studied for which contributed the development and improvement of enrichment techniques. Nowadays, glycoprote...
Article
New magnetic nanoprobes based on surface-functionalized magnetite particles were synthesized and used to selectively capture metalloproteases from human saliva samples. In addition to high specific surface area, these nanoparticles displayed metal ion chelating moieties grafted onto the surface due to the reaction with the organosilane EDTA-TMS ((N...
Article
Formed as an interdisciplinary domain on the basis of Human Genome Project, proteomics aims at the large-scale study of proteins. The enthusiasm that resulted from obtaining the complete human genetic information has, however, been chastened by the realization that this information contributes little to the comprehension and knowledge of the expres...

Questions

Questions (2)
Question
Hello,
I am dialyzing a solution of Carbon dots produced using ethylediamine and citric acid. Although some of them stay inside the dialysis membrane, a part of them is being released to the external solution. Is this normal? I am using a float-A-Lyzer 500-1000 Da device.
This is a problem for me, because I am creating a bias in my system (only the biggest ones will remain) and I cannot estimate a true Yield (by mass).
Thank you
Question
Hello,
I am synthesizing nanoparticles. In my recipe I have to use iron (III) and tetradecyl phosphonic acid. My problem is that in the end, when I go to the microscopy I always have excess of organic material, which is composed by the complex iron-phosphonic acid (EDS shows iron and phosphorous).
I already tried wash them with 1-propanol, acetone, chloroform, hexane and other typical solvents but it seems that none of these solvents is able to wash this complex. 
I usually centrifuge the particles between 4k rpm and 6.5k rpm.
Thank you for your help
Rui

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Projects

Projects (2)
Archived project
Development of Carbon dot-based materials for bioscience applications.