Rui Miguel Magalhães

Rui Miguel Magalhães
Universidade Católica Portuguesa | UCP · Centro de Biotecnologia e Química Fina

BSc

About

34
Publications
4,492
Reads
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594
Citations
Citations since 2016
18 Research Items
480 Citations
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2016201720182019202020212022020406080100
2016201720182019202020212022020406080100
2016201720182019202020212022020406080100

Publications

Publications (34)
Chapter
Listeria monocytogenes is a ubiquitous microorganism responsible for listeriosis, a severe disease in humans that become infected by ingesting contaminated foods. Although it can occur in healthy humans, listeriosis affects mainly the elderly, immuno-compromised, pregnant women and newborns, with a high case fatality rate (20%–30%). This article wi...
Article
Full-text available
Water contaminated with microbiological and chemical constituents can cause a variety of diseases. Water bodies may become contaminated by wild and domestic animal feces, agricultural runoff or sewage, and are often overlooked as a reservoir and source of human infection by pathogenic microorganisms. The objectives of this study were to evaluate th...
Article
Full-text available
Epidemiological studies show that improper food handling practices at home account for a significant portion of foodborne illness cases. Mishandling of raw meat during meal preparation is one of the most frequent hazardous behaviours reported in observational research studies that potentially contributes to illness occurrence, particularly through...
Chapter
Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples rely on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.
Article
Full-text available
The aim of this study was to conduct a preliminary investigation on the occurrence of Salmonella spp. in eggs from chickens raised in backyards in Portugal and Romania. A lack of compliance with safety practices by chicken owners, was demonstrated, especially in Portugal, as 96% of the eggs were visibly dirty and 92.5% were stored at room temperatu...
Article
Full-text available
The objective of this work was to investigate the effect of stress conditions frequently encountered in food- associated environments on virulence-associated characteristics of eight strains of Listeria monocytogenes. Strains were grown at low (11 °C, cold stress) and optimal (37 °C) temperatures and in high NaCl concentrations (6% NaCl, 11 °C; col...
Article
Full-text available
We report here the draft genome sequence of Listeria monocytogenes 2542, a serotype 4b clinical strain recovered from a placental sample during a cheese-related listeriosis outbreak in Portugal.
Article
The association of alginate beads and guar-gum films in a single delivery system was idealized to promote a more effective buccal and oral delivery of bioactive molecules. A response surface method (experimental design approach) was performed to obtain optimal formulations of alginate beads to be incorporated into guar gum oral films as combined bu...
Article
Naturally fermented meat sausages have a long tradition in Mediterranean countries and are one of the most important groups of traditional foods consumed throughout Europe. Despite all the advances in food science and technology and increased regulatory requirements and concerns for safety and quality during the last decades, the challenge to contr...
Article
The potential of constructed wetlands (CWs) as a low technology for wastewater treatment is timely but there is a need to understand the route of pathogenic bacteria (Listeria monocytogenes and Salmonella spp.) and indicator organisms (Enterobacteriaceae and Escherichia coli) present in wastewater within those systems and to what extent they can co...
Article
Full-text available
Contaminated food with Listeria monocytogenes is the predominant route of transmission of listeriosis to humans, a severe illness with a high mortality rate. Food processing environments can be colonized by persistent strains, repeatedly isolated for months or years. This study aimed to investigate the biofilm formation capacity of six strains of L...
Article
The ongoing rise of antibiotic resistant microbial pathogens has become one of the major public health threats worldwide. Despite all the effort and actions taken so far, a proliferation of antibiotic resistant (AR) and multi-antibiotic resistant (MAR) strains is still observed, including in foodborne pathogens. This trend has been also noted recen...
Article
Listeria monocytogenes is a food-borne pathogen responsible for outbreaks and sporadic cases of listeriosis, a severe invasive disease. Internalin A (InlA) a protein encoded by inlA has a key role in the mechanism of pathogenesis in L. monocytogenes infection, specifically in the invasion of human intestinal epithelial cells. Studies on inlA have s...
Article
This study aimed to investigate the effect of different conditions, including temperature (37 ºC, 22 ºC, and 4 ºC), NaCl concentrations (2.5%, 4%, and 8%), and acidity (pH = 5), on the growth response of persistent and non-persistent isolates of Listeria monocytogenes. The resistance to two common sanitizers (benzalkonium chloride and hydrogen pero...
Chapter
A significant challenge for traditional food production is to improve its competitiveness by identifying innovations that guarantee the safety of the products, while at the same time meeting general consumer demands and specific consumer expectations towards traditional food (European Research on Traditional Food, EC, DG_Research, 2007). Therefore,...
Article
In Portugal, listeriosis has been notifiable since April 2014, but there is no active surveillance programme for the disease. A retrospective study involving 25 national hospitals led to the detection of an outbreak that occurred between March 2009 and February 2012. The amount of time between the start of the outbreak and its detection was 16 mont...
Article
Full-text available
Listeria monocytogenes is a ubiquitous microorganism responsible for listeriosis, a rare but severe disease in humans, who can become infected by ingesting contaminated food products, namely dairy, meat, fish, and vegetables. Although it can occur in healthy humans, listeriosis mainly affects the elderly, immunocompromised, persons pregnant women,...
Article
Full-text available
Abstract Listeria monocytogenes infection (listeriosis) is an uncommon but severe foodborne illness that affects mainly individuals with recognized underlying conditions: the elderly, immunocompromised individuals, and pregnant women and their fetuses. The aim of this study was to obtain epidemiological data on cases of listeriosis occurring in Por...
Article
Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.
Article
Listeria monocytogenes is a ubiquitous bacterium widely distributed in the environment that can cause a severe disease in humans when contaminated foods are ingested. Cheese has been implicated in sporadic cases and in outbreaks of listeriosis worldwide. Environmental contamination, in several occasions by persistent strains, has been considered an...
Article
Abstract The aim of this study was to characterize a broad collection of isolates of Listeria monocytogenes, of different serotypes, recovered in Portugal between 2003 and 2007 from foods (n=353) and from clinical cases of human listeriosis (n=95), in terms of antimicrobial susceptibility. All the isolates were susceptible to ampicillin, the prefer...
Article
Full-text available
Objective. A total of 725 Listeria monocytogenes isolates, 607 from various foods and 118 from clinical cases of listeriosis, were investigated concerning their ability to form biofilms, at 4°C during 5 days and at 37°C during 24 h. Methods. Biofilm production was carried out on polystyrene tissue culture plates. Five L. monocytogenes isolates were...
Article
The effects of previous exposure to sub-lethal acidic and osmotic stresses on the survival of Listeria monocytogenes during exposure to gastro-intestinal (GI) tract simulation, was investigated. Six L. monocytogenes strains isolated from cheeses were selected and exposed to high salt concentrations or acidic conditions and their viability compared...
Conference Paper
Pathogenic microorganisms such as Listeria monocytogenes are known to form biofilms. The risk of contamination of food products in the food industry and growth on medical devices in the clinical environment is a cause of concern. The objectives of this work were to examine and compare biofilm formation by food and clinical isolates of L. monocytoge...
Article
Full-text available
In recent years, the number of cases of listeriosis has increased worldwide. Ninety-five isolates of Listeria monocytogenes recovered from Portuguese human cases of listeriosis have been characterized by biotyping (cadmium and arsenic sensitivity), polymerase chain reaction (PCR) grouping, and by pulsed-field gel electrophoresis (PFGE) applying the...
Article
This study was conducted to evaluate the ability of Pediococcus acidilactici HA-6111-2, a PA-1 bacteriocin-producing lactic acid bacterium (LAB), isolated from “Alheira” to inhibit a cocktail of Listeria innocua strains during production and shelf-life of these products. The bacteriocinogenic culture reduced the Listeria population below the detect...

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Projects

Projects (2)
Project
Funded by Horizon2020 and coordinated by Nofima. The project will run for five years with 32 partners in 14 countries in Europe. The overall objective is to reduce health burden from foodborne illnesses. http://safeconsume.eu/