Rui Hou

• 湖南工学院

Objective To clarify whether the 3D printing model has auxiliary functions in toto extraction of donor tooth in autotransplantation cases. Methods Two hundred and sixty patients who would have operation of ATT were divided into two groups. In group 1, determination of the tooth extraction in toto was predicted only according to the clinical and im...

Objectives Odontogenic inflammatory diseases are main causes for alveolar bone breakdown and teeth loss, leaving great difficulties in denture restoration. Local inflammatory granulation tissue (IGT) is considered as pathological tissue and required to be removed. However, there are many evidences supporting that under appropriate intervention, IGT...

Objective: A case of primary first bite syndrome (FBS), diagnosed in a patient with nonspecific adenocarcinoma of the deep lobe of the parotid gland. Data sources: A Medline literature search was conducted on PubMed, using the keywords "first bite syndrome." Review methods: Using primary FBS and existence of a definite etiology as inclusion cr...

Immediate hypersensitivity reaction (IHR) can be divided into allergic- and non-allergic-mediated, while “anaphylaxis” is reserved for severe IHR. Clinically, true penicillin allergy is rare and most reported penicillin allergy is “spurious”. Penicillin-initiated anaphylaxis is possible to occur in skin test- and specific IgE-negative patients. The...

Amlexanox, an anti-inflammatory agent, is widely used for treating aphthous ulcers. Recently, amlexanox has received considerable attention because of its efficacy in mitigating metabolic inflammation via directly suppressing IKKε/TANK binding kinase 1 (TBK1). However, because the knockdown of IKKε/TBK1 had no anti-inflammatory effect on lipopolysa...

The present study evaluated the effects of calcitonin gene-related peptide (CGRP) on bone marrow mesenchymal stem cells (BMMSCs) in vitro and in a rat model of mandibular distraction osteogenesis (MDO). Rat BMMSCs were isolated then treated with CGRP or CGRP antagonist (CGRP8-37). The proliferation and migration ability of BMMSCs was determined usi...

Objective: To investigate how a high glucose environment influences the osteogenic ability of periodontal ligament stem cells (PDLSCs) and the function of autophagy in this process, we explored whether the osteogenic ability of PDLSCs could be protected by autophagy. Design: PDLSC proliferation and osteogenesis were evaluated by CCK-8 and wester...

Shuang-Huang-Lian (SHL), an herbal formula of traditional Chinese medicine, is clinically used for bronchial asthma treatment. Our previous study found that SHL prevented basophil activation to suppress Th2 immunity and stabilized mast cells through activating its mitochondrial calcium uniporter. Sporadic clinical reports that SHL was used for the...

Among traditional Chinese medicine injections, intravenous Shuang-Huang-Lian (IV-SHL) has the highest incidence of injection-induced immediate hypersensitivity reactions (IHRs). The precise mechanisms of IV-SHL-induced IHRs remain ambiguous. In this study, we investigated the mechanisms of SHL injection (SHLI)-induced IHRs. Our data showed that ser...

Background Basophilic granulocytes (BGs) not only initiate the induction of Th2 cell differentiation, but also amplify the ongoing Th2 response. Shuang-Huang-Lian (SHL) is clinically used for relieving type I hypersensitivity by continuous treatment for several weeks. MethodsELISA, flow cytometry, magnetic activated cell sorting, isoelectric precip...

Scope: In this study, we investigated the underlying molecular mechanism of the effect of tectochrysin on LPS-primed macrophages. Methods and results: As measured by western blot, RT-PCR and ELISA, tectochrysin inhibited ERK1/2 phosphorylation and sequentially suppressed downstream iNOS, TNF-α and IL-6 transcription as well as the NO, TNF-α and...

The endotoxin lipopolysaccharide (LPS)-induced pulmonary endothelial barrier disruption is a key pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). However, the molecular mechanisms underlying LPS-impaired permeability of pulmonary microvascular endothelial cells (PMVECs) are not fully understood. Gap junctions,...

Obacunone (OBA) is a highly oxygenated triterpenoid with various pharmacological activities. In this study, we explored its anti-inflammatory effect and underlying mechanisms in LPS-activated macrophages. Our data showed that OBA potently decreased pro-inflammatory mediators (e.g. NO, IL-6, IL-1β and MCP-1) at the transcriptional and translational...

Objective: The aim of the present study was to investigate the effect of static strain on bone marrow mesenchymal stem cell (BMMSC) migration and whether the p38/matrix metalloproteinase-2 (MMP-2) axis plays a role in induction of BMMSC migration under mechanical strain. Design: Both in vivo and in vitro investigations were performed. Twelve adu...

Mast cells (MCs) are key effector cells of IgE-FcεRI- or MrgprX2-mediated signaling event. Shuang-Huang-Lian (SHL), a herbal formula from Chinese Pharmacopoeia, has been clinically used in type I hypersensitivity. Our previous study demonstrated that SHL exerted a non-negligible effect on MC stabilization. Herein, we sought to elucidate the molecul...

This study aims to investigate the effects of different concentrations of antioxidant of bamboo leaves (AOB) on mouse embryonic stem (mES) cells and to explore the underlying molecular mechanism by which AOB affects the proliferation and apoptosis of mES cells. Compared with the control group, Oct4 expression was remarkably upregulated and Nanog ex...

Objectives To review the cases of accidental aspiration and ingestion of foreign objects during dental procedure, and to emphasize the importance of thorough documentation of the accidents. Methods A comprehensive search on (dental procedure/treatment/practice), (aspiration/inhalation), and (ingestion/swallow) was performed for all years before 1s...

Distraction osteogenesis (DO) is a widely used self-tissue engineering. However, complications and discomfort due to the long treatment period are still the bottleneck of DO. Novel strategies to accelerate bone formation in DO are still needed. P38 is capable of regulating the osteogenic differentiation of both mesenchymal stem cells (MSCs) and ost...

Aim: To develop the in vitro and in vivo models induced by shrimp tropomyosin (ST) and monoclonal tropomyosin-specific murine IgE antibody (anti-ST-IgE mAb). Methods: ST was purified from Metapenaeusensis by an isoelectric precipitation method. The anti-ST-IgE mAb was obtained from hybridomas. After RBL-2H3 cells were sensitized with anti-ST-IgE mA...

The association between inflammation and endoplasmic reticulum (ER) stress has been described in many diseases. However, if and how chronic inflammation governs the unfolded protein response (UPR) and promotes ER homeostasis of chronic inflammatory disease remains elusive. In this study, chronic inflammation resulted in ER stress in mesenchymal ste...

Objective: To evaluate the transport property of water extract from Magnoliae Officinalis Cortex (WEMOC) across Caco-2 monolayer model by bioactivity method and observe the influence on the activity of P-glycoprotein (P-gp) in cell membrane. Methods: The validated Caco-2 cell monolayer model was used to observe the bidirection transportation of WEM...

microRNAs could be mechanosensitive and emerge as critical posttranscriptional regulators in the bone-remodeling process. During orthodontic tooth movement (OTM), the application of mechanical force induces alveolar bone remodeling, but whether microRNAs respond to orthodontic force and contribute to OTM is unknown. microRNA-21 (miR-21) has been pr...

Our aim was to evaluate the influence of preservation of the alveolar ridge on delayed implants with different defects in the buccal bone. We enrolled 60 patients who had one posterior mandibular tooth extracted. Cone-beam computed tomography (CT) was used to measure the buccal bone defects in the alveolar ridge before the tooth was extracted (leve...

Perineural invasion (PNI) is a striking biological behavior observed in salivary adenoid cystic carcinoma (SACC). The present study was designed to establish a co‑culture model of SACC cells with Schwann cells (SCs), and then study epithelial-mesenchymal transition (EMT) and the Schwann‑like differentiation of SACC cells to investigate the likely m...

Background: Lack of specificity of anti-PGE2 antibodies is a long-standing problem. Given quite a few analogs and low PGE2 content in biological fluids, it is quite important to simultaneously meet the demands of high specificity and sensitivity. Results: Highly specific anti-PGE2 antibodies were obtained by combined use of cationic carrier prot...

Reference genes are commonly used as a reliable approach to normalize the results of quantitative polymerase chain reaction (qPCR), and to reduce errors in the relative quantification of gene expression. Suitable reference genes belonging to numerous functional classes have been identified for various types of species and tissue. However, little is...

Electrical steel laminations used in the transformer cores and motors are subject to stresses (σ) introduced during their construction. The magnetostriction as a major source of acoustic noise of the transformer cores has high stress sensitivity and is harmful to the electrical power grid. The higher harmonics of magnetostriction are more important...

Shuang-Huang-Lian (SHL) is a common traditional Chinese preparation extracted from Lonicerae Japonicae Flos, Scutellariae Radix, and Fructus Forsythiae. In this study, we demonstrate the anti-inflammatory and antioxidative effects of SHL on lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. SHL reduced the lung wet/dry weight ratio...

We investigated the effect of antibiotic prophylaxis on postoperative inflammatory complications after operations for impacted mandibular third molars in Chinese patients. A total of 207 patients had their bilateral third molars removed in a split-mouth, double-blind, self-controlled, clinical trial in two visits. For one side amoxicillin (or clind...

To investigate the effect of antibiotics on postoperative inflammatory complications after surgical extraction of the impacted mandibular third molar. Ninety-Six patients had their bilateral third molars removed through a split-mouth, double-blind, controlled, clinical trial in two visits. On one side amoxicillin (or clindamycin) was used (antibiot...

To evaluate clinically and radiographically an alveolar ridge, preservation technique with deproteinized bovine bone graft and absorbable collagen membrane and then restoration with delayed implants were done. The study included 30 patients. The trial group's sockets were filled with deproteinized bovine bone graft (Bio-Oss) and covered with absorb...

After autotransplantation of teeth the healing of periodontal tissue regulates the patient's prognosis. Umbilical cord mesenchymal stem cells (UCMSC) have shown excellent pluripotent and proliferation potential. In the present study we investigated the characteristics and developmental capability of osteogenic differentiation to find out whether hu...

Objectives The aim of this study is to identify the oral health status as well as oral health practices and access for care of graduating senior high school Tibetan students in Shannan prefecture of Tibet. Methods Based on standards of the 3rd Chinese National Oral Epidemiological Survey and WHO Oral Health Surveys, 1907 graduating students from t...

Objective The articles on the usage of marsupialization and decompression for most cystic lesions of the jaws were reviewed by literature. Methods A computer database search was performed on PubMed and Ovid for all years before 2011 available. Search key words were (marsupialization or decompression), (cyst or cystic) and (dental or oral). The des...

Transient collagen cross-linking treatment by proanthocyanidins (PA), a natural crosslinker, was proved to increase the cross-linking degree and ultimate tensile strength of demineralised dentin. The present study aimed to investigate whether transient pretreatment by PA-based preconditioner can improve the resin-dentin bonds of various etch and ri...

The aim of this retrospective study was to present the findings of an open packing method after enucleation of large keratocystic odontogenic tumors (KCOTs) in the mandible. We performed a retrospective case series study of 27 patients with KCOTs larger than 5 cm treated at our institution between September 2003 and September 2008. A conservative s...

The purpose of this study was to investigate the effect of a portable video eyewear entertainment system used in conjunction with nitrous oxide/oxygen sedation during the removal of impacted lower third molars. Thirty-eight patients had their bilateral third molars removed under local anesthesia and nitrous oxide/oxygen inhalation sedation in 2 vis...

The authors describe a patient with bilateral Tessier no. 6 and no. 7 clefts. The case consists of macrostomia combined with maxillary, zygomatico-orbito-temporal deformities, dental anomalies and mandibular retrusion. In addition, the cleft is located bilaterally in the maxillary arch with a double deciduous posterior dentition. This is rare. The...

To investigate the incidence and the local factors of impacted permanent teeth, except for the third molar, in Chinese patients through an x-ray study. A total of 548 impacted permanent teeth from panoramic radiographs were studied and recorded according to the patients' gender and age, tooth position, and classification of impaction. The local fac...

Objectives: To evaluate the therapeutic outcome of ultrasound-guided intralesional injection of bleomycin A5 on treatment of venous malformation (VM) in cervical-facial region. Methods: Seventy-five patients (32 male, 43 female), ranging in age from 13 to 60 years old, suffering from VM in cervical-facial region were admitted to and treated at o...

Objectives: Bone remodeling has recently been revealed to be under sympathetic nerve control. The role of the sympathetic nerve system is not clearly understood. The present study aim to explore the effect of chemical sympathectomy and stress on bone remodeling in adult rats. Methods: 24 twelve-month-old Wistar rats were divided into three group...

Mesenchymal stem cell (MSC)-mediated tissue regeneration offers opportunities to regenerate a bio-root and its associated periodontal tissues to restore tooth loss. Previously, we proved that the apical end of developing root was acting as a promising candidate cell source for root/periodontal tissue (R/PT) regeneration. In the present study, we in...

A finite element analysis was carried out to evaluate continuous and simultaneous variations of orthodontic mini-implant diameter and length, and to identify their optimal ranges in the maxillary posterior region. Within the ranges of diameter from 1.0 mm to 2.0 mm and the length from 6.0 mm to 16.0 mm, our results showed that the increases of the...

Inferior alveolar nerve (IAN) injury is a concern in mandible distraction osteogenesis (DO). We have previously demonstrated that repeated local injections of human nerve growth factor beta (NGF-beta) have significantly enhanced the histologic recovery of the IAN in a rabbit model of DO. This study was to further test the effect of a single injecti...

Our objective was to provide the management guidelines for facial nerve schwannomas (FNSs) presenting as a parotid mass. The study is a case report and literature review. Four clinical cases of patients with an asymptomatic parotid mass diagnosed as FNS are presented. The patient's presentation, the diagnostic algorithm and surgical rationale are d...

Previous studies have suggested that periodontal ligament stem cells (PDLSCs) play crucial role in regeneration of periodontal defects, and recently tissue engineering based on PDLSCs to enhance periodontal regeneration has been the focus of periodontal research. A theoretical way to achieve this goal would be to provide a "stimulatory'' environmen...

To observe the outcome of using fibrin glue (FG) combined with Pingyangmycin (Tianjin Taihe Pharmaceutical Co Ltd, Tianjin, China) in the treatment of venous malformations (VMs) in the oral and maxillofacial region. The treatment data of 7 patients with VMs from January 2005 to January 2006 were reviewed. All these patients were injected with FG co...

To evaluate continuous and simultaneous variations of implant diameter and length for an experimental cylinder implant. A finite element model of a mandible segment with implant was created. The range of implant diameter (D) was set from 2.5 to 5.0 mm, and that of implant length (L) from 6.0 to 16.0 mm. The maximum Von Mises stresses in the mandibl...

Fabricate series of the controllable degradation coral-hydroxyapatite. The natural coral undergo a chemical reaction with (NH4)2 HPO4 at high temperature and pressure for different time-lengths. After getting the products, the components and the special structures were analyzed. Observe the biologic degradation of the reaction products and analyze...

Tissue engineered bone has become a bone substitute for the treatment of bone defects in animal research. This study investigated the osteogenesis capacity of coral-MSCs-rhBMP-2 composite with the auto-bone-graft as control. Coral-MSCs-rhBMP-2 composite were fabricated by coral (as main scaffold), rhBMP-2 (as growth factor), and MSCs (cultured from...

To evaluate repair of critical-sized cranial defect with tissue engineered bone fabricated by coral, bone mesenchymal stem cells (MSCs) and sustainedly released recombinant human bone morphogenetic protein 2 (rhBMP-2) by collagen. Three scaffolds of rhBMP-2 + coral, collagen + rhBMP-2 + coral and MSCs+collagen+rhBMP-2+coral were fabricated. Forty N...

To observe the osteogenesis of sodium calcium phosphate/beta-tricalcium phosphate scaffold loaded with marrow stromal cells in a nude mice subcutaneous model. To transform the sintered bovine cancellous bone into NaCaPO(4)/beta-TCP biphasic calcium phosphate ceramic by physical and chemical methods. Bone marrow stromal cells (BMSCs) were obtained b...

To evaluate the long-term effects of rhBMP-2 and rhbFGF on alkaline phosphatase(ALP) of rabbit bone mesenchymal stem cells by using them alone, associatedly and sequentially. Under cell culture technique, ALP kits were used to evaluate ALP level of bone mesenchymal stem cells so as to reflect differentiation of the cells on the 2nd, 5th, 7th, and 1...

[Abstract] Objectives: To evaluate the effect of ultrasound-guided intralesional injection of Pingyangmycin on treatment of venous malformation in cervical-facial region. Methods: Seventy-five patients suffering from venous malformation, including 54 patients in the facial region, 8 patients in the submental region, 10 patients in the submandible r...