Rubén AlmadaCentro de Estudios Avanzados en Fruticultura (CEAF) · Genómica Funcional
Rubén Almada
Doctor of Philosophy
About
37
Publications
6,582
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
421
Citations
Citations since 2017
Introduction
Additional affiliations
January 2010 - November 2015
Centro de Estudios Avanzados en Fruticultura (CEAF)
Position
- Principal Investigator
Publications
Publications (37)
Hexokinases (HXKs) and fructokinases (FRKs) are the only two families of enzymes in plants that have been identified as able to phosphorylate Glucose (Glc) and Fructose (Fru). Glc can only be phosphorylated in plants by HXKs, while Fru can be phosphorylated by either HXKs or FRKs. The various subcellular localizations of HXKs in plants indicate tha...
We used an Open Top Chamber (OTCs) with passive heating for increasing the ambient temperature (around 3-4°C increase) around the adult sweet cherry trees during flowering induction and flower organogenesis (summer months). We analyzed the effect(s) of high temperature stress on the flower development.
We used an Open Top Chamber (OTCs) with passive heating for increasing the ambient temperature (around 3-4°C increase) around the adult sweet cherry trees during flowering induction and flower organogenesis (summer months). We analyzed the effect(s) of high temperature stress on the flower development.
We used Open Top Chambers (OTCs) with passive heating for increasing the ambient temperature ( 3-4°C increase) around the adult sweet cherry trees during flowering induction and flower organogenesis (summer months). We analyzed the effect(s) of high temperature stress on the flower development.
Prunus rootstock belonging to subgenera Amygdalus (peach), Prunus (plum) and Cerasus (cherry) are either from the same species as the scion or another one. The number of inter-species (including inter-subgenera) hybrids has increased as a result of broadening the genetic basis for stress (biotic and abiotic) resistance/tolerance. Identifying genes...
In sweet cherry trees, flowering is commercially important because the flowers, after fertilization, will generate the fruits. In P. avium, the flowering induction and flower organogensis are the first developmental steps towards flower formation and they occur within specialized organs known as floral buds during the summer, nine months before blo...
Genotyping-by-Sequencing (GBS) was applied in a set of 53 diploid Prunus rootstocks and five scion cultivars from three subgenera (Amygdalus, Prunus and Cerasus) for genome-wide SNP identification and to assess genetic diversity of both Chilean and Spanish germplasm collections. A group of 45,382 high quality SNPs (MAF >0.05; missing data <5%) were...
Prunus rootstock belonging to subgenera Amygdalus (peach), Prunus (plum) and Cerasus (cherry) are either from the same species as the scion or another one. The number of inter-species (including inter-subgenera) hybrids have increased as a result of efforts to broaden the genetic basis for biotic and abiotic resistance/tolerance. Identifying genes...
Arbuscular mycorrhizal fungi (AMF) form a mutualistic relationship with 71% of the known plant species. This relationship called arbuscular mycorrhiza (AM), positively and directly influences plants and AMF fitness and have an indirect effect on diversity and community composition of both integrant of the association. The Chilean matorral is a biod...
Modern plant breeding utilizes a high number of molecular markers for segregation analysis, gene mapping and marker discovery to facilitate marker-assisted selection (MAS). Genotyping-by-sequencing (GBS) is a cost effective next-generation sequencing technology that provides a platform for detection of a high number of SNPs in a large sample pool....
In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue cul...
Maqui (Aristotelia chilensis [Molina] Stunz) is a small dioecious tree native to South America with edible fruit characterized by very high antioxidant capacity and anthocyanin content. To preserve maqui as a genetic resource it is essential to study its genetic diversity. However, the complete genome is unknown and only a few gene sequences are av...
List of genotypes from maqui (A. chilensis) used and their location
(PDF)
Total identified SSR markers from maqui (A. chilensis) by MIcroSAtellite (MISA) tool with its respective primer pairs
(XLSX)
Frequency distribution of microsatellite loci of maqui (A. chilensis) by motif length
(PDF)
Putative genes found in partial sequences of maqui (A. chilensis) predicted by Blast2go software
(XLSX)
Putative anthocyanidin-related genes found in partial sequences of maqui (A. chilensis) predicted by Blast2go software
(PDF)
Characteristics of 50 putative nuclear microsatellite loci developed for maqui (A. chilensis).
For each locus, the forward and reverse primer sequences, repeat motif and annealing temperature when run individually (Ta) are shown.
(PDF)
Distribution of identified SSRs from maqui (A. chilensis) using MISA software according to SSR motif type and repeat number
(PDF)
The aim of this study was to amplify the second intron of S-RNase from Prunus genotypes used as rootstocks from the germplasm collection at CEAF, to identify S-alleles and to determine the S-genotype of some diploid rootstocks, as a first approach to establish a methodology for further studies.
Root hypoxia in fruit trees affects growth, vegetative development, and reproductive development, which is reflected in low productivity, poor fruit quality, and premature decay of trees. Using Illumina Hiseq2000, we performed transcriptome analysis of roots from two different rootstocks, ‘Mariana 2624’ and ‘Mazzard F12/1,’ which are tolerant and s...
Root hypoxia produced by flooding or over-irrigation limits stone fruit tree development, particularly in orchards established on soils with restricted drainage. To overcome this problem, stone fruit trees are usually grafted on rootstocks (species or hybrid of the Prunus L. genus) with different degrees of tolerance to root hypoxia. However, the m...
A cytotaxonomical description of Cyclopogon (Spiranthinae, Orchidaceae) is carried out through a deep karyotype analysis of four species from NE Argentina. Distinctive karyotype parameters concerning the chromosomes number, morphology, size and symmetry and the genome size associate to each taxon. Cyclopogon calophyllus (2n = 2x =28; 18m + 10sm), C...
Stone fruit (Prunus L.) production in Chile covers ≈ 43,000 ha and includes a wide variety of soils and climates requiring a large diversity of rootstocks. The most commercially important rootstock cultivars are 26 genotypes from three different taxonomic groups belonging to the subgenera Amygdalus (L.) Benth. Hook. (peach group), Prunus Focke [= P...
Wild and cultivated Fragaria chiloensis ssp. chiloensis (Fcc) plants were collected at different locations in southern Chile in order to determine the current viral status of this native strawberry. The following aphidborne viruses (ABVs): Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV) and...
Grapevine sexual reproduction involves a seasonal separation between inflorescence primordia (flowering induction) and flower development. We hypothesized that a repression mechanism implicating epigenetic changes could play a role in the seasonal separation of these two developmental processes in grapevine. Therefore, the expression of five grapev...
The center of diversity of Argentinean orchids is in the northeast region of the country. Chromosome numbers and karyotype features of 43 species belonging to 28 genera are presented here. Five chromosome records are the first ones at the genus level; these taxa are Aspidogyne kuckzinskii (2n = 42), Eurystyles actinosophila (2n = 56), Skeptrostachy...
Two previously uncharacterized Vitis vinifera CONSTANS-like genes (VvCO, VvCOL1), which are predicted to encode proteins with homology to members of the Arabidopis CONSTANS family, were identified. Under controlled conditions, both genes show a diurnal expression pattern with peak at dawn. During grapevine bud development, VvCOL1 is mainly expresse...
Karyotypes of 18 populations belonging to eight of the southernmost species of South American Crotalaria were examined. All had x = 8, except C. incana which had x = 7. Diploid, tetraploid and octoploid species were found. The octoploid is a new record and is the highest ploidy level detected so far in the genus. Species within the same ploidy leve...
In this study, mitotic and meiotic counts are informed for 10 species that belong to 5 out of the 7 sections represented in Argentina. The chromosome numbers of E. coronatum (n=8) and E. pristis (2n = 16) constitute the first counts for the species, chromosome numbers that differ from previous counts are given for some species, and counts for other...
Projects
Projects (4)
Since there are few studies on floral biology in sweet cherry, this project aims to deep insight into the bud organogenesis (by means of histology studies), molecular (by means of floral bud transcriptome, gene expression patterns and gene promoter functional analyses) and hormonal regulation (by means of the determination of phytohormone profiles of floral buds and exogenous application of hormones) of flowering induction and floral bud development in P. avium.
To deep insight into the histological, molecular and hormonal regulation of flowering induction and floral bud development in P. avium trees under high temperature.
To describe, for the very first time, patterns of diversity and distribution of AMF present in the soil and in the roots of plants of a sclerophyll forest in the central zone of Chile and how those ecological patterns are affected by host plant species, soil physicochemical factors and seasons of the year. For this aim I use classical morphological and molecular approaches of AMF to describe diversity.
