Ru Zhang

Ru Zhang
Northwestern Polytechnical University | NWPU · School of Ecology and Environment

PHD

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23
Publications
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613
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Publications

Publications (23)
Article
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Swallowtail butterflies (Papilionidae) are a historically significant butterfly group due to their colorful wing patterns, extensive morphological diversity, and phylogenetically important position as a sister group to all other butterflies and have been widely studied regarding ecological adaption, phylogeny, genetics, and evolution. Notably, they...
Article
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Ruminants comprise a highly successful group of mammals with striking morphological innovations, including the presence of a rumen. Many studies have shown that species-specific or lineage-specific genes (referred to as new genes) play important roles in phenotypic evolution. In this study, we identified 1064 ruminant-specific genes based on the ne...
Article
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Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing numbe...
Article
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The nearly complete mitochondrial genome (mitogenome) of Xenos cf. moutoni, one twisted-wing parasite on wasp Vespa velutina from Southwest of China, is described in this study. The total length of this mitogenome is 16,717 bp, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and an incomplet...
Article
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Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive. Especially, the genic basis of luciferin (D-luciferin) biosynthesis and light patterns is largely unknown. Here, we present the high-quality reference genomes of two fireflies Lamprigera yunnana (10...
Article
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Artificially improving traits of cultivated alfalfa (Medicago sativa L.), one of the most important forage crops, is challenging due to the lack of a reference genome and an efficient genome editing protocol, which mainly result from its autotetraploidy and self-incompatibility. Here, we generate an allele-aware chromosome-level genome assembly for...
Article
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The leaf resemblance of Kallima (Nymphalidae) butterflies is an important ecological adaptive mechanism that increases survival. However, the genetic mechanism underlying ecological adaptation remains unclear owing to a dearth of genomic information. Herein, we determined the karyotype (n = 31) of the dead‐leaf butterfly Kallima inachus, and genera...
Article
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Butterflies have been of great interest to naturalists for centuries, and the study of butterflies has been an integral part of ecology and evolution ever since Darwin proposed his theory of natural selection in 1859. There are > 18 000 butterfly species worldwide, showing great diversity in morphological traits and ecological niches. Compared with...
Article
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Background Papilio bianor Cramer, 1777 (commonly known as the Chinese peacock butterfly) (Insecta, Lepidoptera, Papilionidae) is a widely distributed swallowtail butterfly with a wide number of geographic populations ranging from the southeast of Russia to China, Japan, India, Vietnam, Myanmar, and Thailand. Its wing color consists of both pigmenta...
Article
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asparagus bean (Vigna. unguiculata ssp. sesquipedialis), known for its very long and tender green pods, is an important vegetable crop broadly grown in the developing asian countries. In this study, we reported a 632.8 Mb assembly (549.81 Mb non-N size) of asparagus bean based on the whole genome shotgun sequencing strategy. We also generated a lin...
Article
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The ruminants are one of the most successful mammalian lineages, exhibiting morphological and habitat diversity and containing several key livestock species. To better understand their evolution, we generated and analyzed de novo assembled genomes of 44 ruminant species, representing all six Ruminantia families. We used these genomes to create a ti...
Article
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Lamprigera (Lampyridae) is a small genus with only 17 species distributing in Asian countries. Its larviform females and alate males can produce continuously strong yellow‐green light at night. However, no luciferase gene was reported for this genus and its subfamily‐level phylogenetic position still remains uncertain. Here, we cloned the luciferas...
Data
Data S2 The NtPDS sequences of Figure 4 (Experiment 1) and Figure S15 (Experiment 2 and 3).
Data
Data S4 The NtPDS sequences of Figure 7 (Exp2R1) and Table 4.
Data
Data S1 The NtPDS sequences of Figure 3 (Experiment 1) and Table 2.
Data
Data S3 The sequences of Zea mays single‐cell ZmIPK genes in Figure 5.
Data
Figure S1 Protoplast transfection of various species. Figure S2 Construction of pCAMBIA1300‐OsU3‐Cas9. Figure S3 Targeted mutagenesis of Bambusa oldhamii protoplasts. Figure S4 The putative mutated BoPDS PCR products from Bambusa oldhamii protoplasts were cloned and validated by sequencing. Figure S5 Targeted mutagenesis of Setaria italica prot...
Data
Table S1. Protocol for protoplast isolation and PEG transformation of different Poaceae species. Table S2 Protocol for protoplast isolation and PEG transformation of different Brassicaceae species. Table S3 Protocol for protoplast isolation and PEG transformation of different Solanaceae species.
Article
Full-text available
Rice yield is an important and complex agronomic trait controlled by multiple genes. In recent decades, dozens of yield-associated genes in rice have been cloned, many of which can increase production in the form of loss or degeneration of function. However, mutations occurring randomly under natural conditions have provided very limited genetic re...
Article
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Plant protoplasts are useful for assessing the efficiency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR Associated Protein 9 (Cas9) mutagenesis. We improved the process of protoplast isolation and transfection of several plant species. We also developed a method to isolate and regenerate single mutagenized Nicotianna...

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Projects (5)