Roya Pournejati

Roya Pournejati
Shiraz University · Department of Biology

PhD in Cell and Molecular Biology

About

12
Publications
2,196
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
90
Citations
Introduction
In my Ph.D. project, I worked on “natural product” from microorganism mainly focused on finding novel bioactive metabolites from marine bacteria. After conducting a local marine bacterial isolation from the Persian Gulf and screening them for bioactive natural products, we chose two bacterial isolates belong to Bacillus velezensis and Microbacterium sp. for further analysis. I succeeded to purify, characterize and identify four cytotoxic secondary metabolites and one antibacterial compound.

Publications

Publications (12)
Article
In the present work, a novel series of monocyclic 1H‐phenanthro[9,10‐d]imidazole β‐lactam hybrids has been synthesized in yields varying from 70–95 % by a stereoselective [2+2]‐cyclocondensation (Staudinger reaction). The newly‐synthesized compounds and their imine intermediates were characterized by IR, 1H NMR, 13C NMR and elemental analyses. The...
Article
In the present paper, we investigated the effect of pure, Ag- deposited, coated, and doped self-organized titanium dioxide (TiO2) nanotubes (NTs) on the growth process of cancer cells, considering the effects of geometry, crystallinity, toxicity, and photocatalytic activity of TiO2 -NTs (TNTs). The TNTs were fabricated by anodizing titanium foil. T...
Article
Full-text available
Increasing concerns about the emergence of antibiotic-resistant bacteria have drawn much attention for research efforts to introduce new types of antibacterial compounds among scientists. In the present report, 24 bacterial strains isolated from the coastal areas of the Persian Gulf in Iran were investigated in order to assess their potentials to p...
Article
Increasing concerns about the emergence of antibiotic-resistant bacteria have drawn much attention for research efforts to introduce new types of antibacterial compounds among scientists. In the present report, 24 bacterial strains isolated from the coastal areas of the Persian Gulf in Iran were investigated in order to assess their potentials to p...
Article
Phthalates are widely used in polymer science and have potential toxicity related to their chemical structures. However, lots of evidence indicate that phthalate derivatives are undoubtedly produced as secondary metabolites by organisms, including plants, animals, and microorganisms. In the present study, Bacillus velezensis strain RP137 was cultur...
Article
Full-text available
Screening of bioactive compounds with potential binding affinity to DNA as one of the target molecules in fighting against cancer cells has gained the attention of many scientists. Finding such compounds in the cellular content of microorganisms, especially marine bacteria as valuable and rich natural resources, is of great importance. Microbacteri...
Article
Background: Isolation, introduction and producing bioactive compounds from bacteria, especially marine bacteria, is an attractive research area. One of the main challenges of using these metabolites as drug and their industrialization is the optimization of production conditions. Methods: In the present study, the response surface methodology wa...
Article
Full-text available
Given antibiotic resistance in pathogens, finding antibiotics from new sources is always a topic of interest to scientists. In the present study, among various isolates from the Persian Gulf coastal area, the strain RP137 was selected as producer of antibacterial compound. Morphological and biochemical studies along with 16S rDNA sequencing showed...
Article
Full-text available
This study describes our investigations on the synthesis, molecular modeling, and biological capabilities of a new β‐lactam containing the anticancer agent, amonafide. This is the first time that amonafide has been applied to a β‐lactam synthesis, which involved five steps from naphthalic anhydride. The β‐lactam was structurally characterized by FT...
Article
Highly diastereoselective synthesis of some novel benzothiazole-substituted β-lactam hybrids was achieved starting from (benzo[d]thiazol-2-yl)phenol as an available precursor. This is the first time (benzo[d]thiazol-2-yl)phenoxyacetic acid has been used as ketene source in synthesizing monocyclic 2-azetidinones. These compounds were evaluated for t...
Article
Two water-soluble mono-nuclear macrocyclic lanthanum(III) complexes of 2,6-diformyl-4-methylphenol with 1,3-diamino-2-propanol (C1) or 1,3-propylenediamine (C2) were synthesized and characterized by UV–Vis, FT-IR, ¹³C and ¹H NMR spectroscopy and elemental analysis. C1 complex was structurally characterized by single-crystal X-ray diffraction, which...

Questions

Questions (2)
Question
I want to perform an c18 coloumn quality control to find out whether my coulmn works. however I have no Idea of which chemical should I choose to run . I wanna use water: methanol as mobile phase.
Question
I attempted to purify my aimed compound with column chromatography (silica gel 60) but I failed. Although I wash the column for like a mount with n-hexan - EtOAc in different proportions (data from TLC shows that by the time we used ethyl acetate as mobile phase, the desired compound move with Rf = 0.7), at the end my compound was sticked to the the silica and I could not wash it down. What would you suggest me to do for the purification?

Network

Cited By

Projects

Projects (2)
Project
We conducted a gram positive bacterial isolates library originated from Persian gulf shoreline and start a project of screening for novel bio-active metabolites e.g. Antibacterial, anti inflammatory, antioxidant, DNA nucleas and cytotoxicity potentials. the optimal goal is to characterized bio-active metabolite produced by local microorganisms. until now our lab succeeded to purify, characterize and identify four cytotoxic secondary metabolites and one antibacterial compound. Among them was a novel indole derivative compound which induces toxicity through DNA damage.