Roxana Myers

Roxana Myers
United States Department of Agriculture | USDA · Agricultural Research Service (ARS)

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26
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Publications

Publications (26)
Article
Okinawan sweet potato, Ipomoea batatas, is an important food staple and export crop for the Island of Hawaii. Cylas formicarius elegantulus, sweet potato weevil, is a major quarantine pest that causes severe destruction to the crop. Root malformation and a bitter taste occur when larvae feed and tunnel within the storage root. Off-grade roots are o...
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Rotylenchulus reniformis is an agricultural pest of economic and regulatory importance. The effects of irradiation on second-stage juveniles (J2) of R. reniformis were evaluated in a glasshouse bioassay. Nematodes were irradiated in an aqueous solution in plastic tubes within cored Ipomoea batatas roots. Radiation treatment doses ranged from 100 to...
Article
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Anthurium amnicola Dressler possesses a number of desirable and novel ornamental traits such as a purple-colored upright spathe, profuse flowering, and floral scent, some of which have been introgressed into modern Anthurium cultivars. As a first step in identifying genes associated with these traits, the transcriptome from root, leaf, spathe, and...
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The coffee berry borer (CBB), Hypothenemus hampei, is the most significant insect pest of coffee worldwide. Since CBB was detected in Puerto Rico in 2007 and Hawaii in 2010, coffee growers from these islands are facing increased costs, reduced coffee quality, and increased pest management challenges. Here, we outline the CBB situation, and summariz...
Article
Edible ginger and turmeric roots are important agricultural commodities for the State of Hawaii. Bacterial wilt, Ralstonia solanacearum, and root-knot nematodes, Meloidogyne spp., are major factors hindering optimum production. An evaluation of tolerance and resistance to M. incognita was undertaken with 22 commercially grown cultivars. Curcuma lon...
Article
A survey of entomopathogenic nematodes (EPN) in the Hawaiian Islands recovered Heterorhabditis indica and previously undescribed Heterorhabditis species. Using Galleria bait, morbid larvae were recovered from 187 of the 275 samples collected within 100 m of the ocean. Entomopathogenic nematodes were recovered from 21% of morbid larvae. Heterorhabdi...
Article
Predatory behavior of a nematode is usually determined through gut content observation or prey delimitation counts. In this experiment, Mononchus and Neoactinolaimus predation of Rotylenchulus reniformis or Meloidogyne incognita was determined using a PCR-based nematode gut content analysis. Soil samples naturally infested with Mononchus were place...
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Nuclear and chloroplast genetic markers have been extensively used for plant identification and molecular taxonomy studies. The efficacy of genetic markers to be used as DNA barcodes is under constant evaluation and improvement with identification of new barcodes that provide greater resolution and efficiency of amplification for specific species g...
Article
Basil oil, Ocimum basilicum L., is a volatile plant essential oil that is known to have insecticidal activity against stored product pests such as rice weevil, Sitophilus oryzae (L.). Basil oil was diluted in acetone and applied to a sponge held inside a tea strainer for fumigations in containers with and without rice. Basil oil fumigation (3 ml, 1...
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A protocol for molecular gut analysis of nematodes was developed to determine if predatory and omnivorous nematodes from five different guilds prey on Rotylenchulus reniformis, Meloidogyne incognita, and Radopholus similis. Mononchoides, Mononchus, Neoactinolaimus, Mesodorylaimus, and Aporcelaimellus were collected from Hawaii agroecosystems. Gut c...
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2013. Detection of plant-parasitic nematode DNA in the gut of predatory and omnivorous nematodes. Nematropica 43:44-48. A protocol for molecular gut analysis of nematodes was developed to determine if predatory and omnivorous nematodes from five different guilds prey on Rotylenchulus reniformis, Meloidogyne incognita, and Radopholus similis. Mononc...
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Key message There are multiple publications on Anthurium transformation, yet a commercial product has not been achieved. This may be due to use of non-optimum promoters here we address this problem. Abstract Different promoters and tissue types were evaluated for transient β-glucuronidase (GUS) expression in Anthurium andraeanum Hort. ‘Marian Seefu...
Article
Evidence is provided here that Steinernema carpocapsae, an entomopathogenic nematode, has the ability to both enter fresh coffee berries, and kill different life stages of the coffee berry borer (Hypothenemus hampei) both in the laboratory and field. In Hawai'i, CBB was reported in 2010, and currently importation of all commercial nematode species...
Article
In Hawaii, reniform nematode, Rotylenchulus reniformis, damages many agricultural crops and disrupts plant trade because of the regulations imposed against this pest by the state of California and other national and international markets. Nurseries in Hawaii must follow strict certification requirements to export potted plants to these markets wher...
Article
A continuous hot water drench treatment was evaluated for disinfesting potted dracaena of reniform nematodes, Rotylenchulus reniformis. Modifications were made to a hot water shower container to allow the delivery of a continuous stream of hot water directly to the media and roots of infested plants. Reniform nematodes were successfully eliminated...
Article
Meloidogyne konaensis causes severe damage to the root systems of Coffea arabica cv. Typica 'Guatemala' grown in Kona, Hawaii. Farmers currently employ grafting of the nematode tolerant C. liberica var. dewevrei 'Fukunaga' to C. arabica cv. Typica scions. Greenhouse experiments confirmed C. liberica 's tolerance to M. konaensis and M. hapla infecti...
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Palmarosa (Cymbopogon martini), lemongrass (C. citratus), and eucalyptus (Eucalyptus globulus) oils were investigated for their effects on Ralstonia solanacearum race 4 and their potential use as biofumigants for reducing bacterial wilt disease of edible ginger (Zingiber officinale). Three concentrations of the oils (0.04, 0.07, and 0.14% vol/vol)...

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