Rowena F Stern

Rowena F Stern
Marine Biological Association of the UK | MBA · CPR survey

PhD

About

52
Publications
14,532
Reads
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1,574
Citations
Additional affiliations
March 2006 - February 2009
University of British Columbia - Vancouver
Position
  • PostDoc Position
Description
  • Diversity of various microalgae groups- mainly on ecologically important dinoflagellates, using DNA barcoding. In collaboration with many culture collections a large barcode database was created to identify unknown environmental species.

Publications

Publications (52)
Article
First routinely deployed in 1931 the Continuous Plankton Recorder (CPR) technology has established the most extensive, marine biological sampling programme in the world. With more than 90 years of sampling, over a total of 8 million nautical miles covered and 500 000 curated samples, the CPR survey provides a gold mine of information available to m...
Chapter
In the last 10 years there has been an explosion of new taxa (from species to phyla) observed using DNA-based identification tools. These tools are useful for identifying morphologically challenging and “hidden” taxa to complement light microscopy observations. Frequently, DNA tools, such as metabarcoding are used to identify whole communities of t...
Article
Full-text available
The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated...
Preprint
Warming, loss of sea icea and changes in ocean currents in the Arctic has led to biochemical changes in pelagic systems that propagate into, and disrupt the Arctic food web. The responses of plankton to environmental variability is critical in understanding how climate change may shape the structure of pelagic ecosystems in the Arctic. To further t...
Article
There is a large diversity of eukaryotic symbionts of copepods, dominated by epizootic protists such as ciliates, and metazoan parasites. Eukaryotic endoparasites, copepod-associated bacteria, and viruses are less well known, partly due to technical limitations. However, new molecular techniques, combined with a range of other approaches, provide a...
Article
Full-text available
Did you know that microbes, too small for the human eye to see, far outnumber and outweigh all animals? Microbes that live in the Arctic carry out a surprising variety of roles recycling food. Despite the cold temperatures, Arctic waters are nutrient rich, which allows a type of microbe called single-celled algae to grow in huge numbers. Only cold-...
Article
Full-text available
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
Full-text available
Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genet...
Poster
Full-text available
This study presents the first metabarcoding results from pilot studies investigating the marine prokaryotic and fungal communities at the Scottish Coastal Observatory monitoring site at Stonehaven.
Article
Several species of the marine diatom Pseudo-nitzschia can produce the neurotoxin domoic acid that is responsible for the seafood-borne illness amnesic shellfish poisoning in humans, marine wildlife mortalities and prolonged closures of fisheries resulting in economic losses to coastal communities. Since the year 2000, Pseudo-nitzschia species have...
Article
Full-text available
Protists (microbial eukaryotes) are diverse, major components of marine ecosystems, and are fundamental to ecosystem services. In the last 10 years, molecular studies have highlighted substantial novel diversity in marine systems including sequences with no taxonomic context. At the same time, many known protists remain without a DNA identity. Sinc...
Article
Two monospecific genera of marine benthic dinoflagellates, Adenoides and Pseudadenoides, have unusual thecal tabulation patterns (lack of cingular plates in the former; and no precingular plates and a complete posterior intercalary plate series in the latter) and are thus difficult to place within a phylogenetic framework. Although both genera shar...
Article
Taxonomic information provides a crucial understanding of the most basic component of biodiversity – which organisms are present in a region or ecosystem. Taxonomy, however, is a discipline in decline, at times perceived as ‘obsolete’ due to technical advances in science, and with fewer trained taxonomists and analysts emerging each year to replace...
Article
Full-text available
Scientific debate on whether or not the recent increase in reports of jellyfish outbreaks represents a true rise in their abundance has outlined a lack of reliable records of Cnidaria and Ctenophora. Here we describe different jellyfish data sets produced within the EU programme EURO-BASIN. These data were assembled with the aim of creating an impr...
Article
Full-text available
Photosynthetic eukaryotes have a critical role as the main producers in most ecosystems of the biosphere. The ongoing environmental metabarcoding revolution opens the perspective for holistic eco-systems biological studies of these organisms, in particular the unicellular microalgae that often lack distinctive morphological characters and have comp...
Article
Full-text available
Scientific debate on whether or not the recent increase in reports of jellyfish outbreaks represents a true rise in their abundance has outlined a lack of reliable records of Cnidaria and Ctenophora. Here we describe different jellyfish data sets produced within the EU programme EURO-BASIN. These data were assembled with the aim of creating an impr...
Article
The phylogeny of ligulate and sulfuric-acid containing species of Desmarestia, occurring worldwide from polar to temperate regions, was revised using a multi-genic and polyphasic approach. Sequence data, gametophyte characteristics and sporophyte morphology support reducing a total of 16 taxa to four different species. (1) D. herbacea, containing b...
Data
Full-text available
Group-specific barcodes for selected genera representing all eukaryotic supergroups (in brackets, number of corresponding sequences in the GenBank). NM, nucleomorph origin. Variable regions used in 18S and 28S genes are indicated in some cases. (PDF)
Data
Full-text available
Number of catalogued morphospecies and V4 18S rDNA OTU-97% among the 60 main eukaryotic lineages. (PDF)
Article
Full-text available
A group of protist experts proposes a two-step DNA barcoding approach, comprising a universal eukaryotic pre-barcode followed by group-specific barcodes, to unveil the hidden biodiversity of microbial eukaryotes.
Article
Full-text available
Background: DNA barcoding offers an efficient way to determine species identification and to measure biodiversity. For dinoflagellates, an ancient alveolate group of about 2000 described extant species, DNA barcoding studies have revealed large amounts of unrecognized species diversity, most of which is not represented in culture collections. To d...
Data
Neighbour-joining phylogenetic analysis of Alexandrium ITS DNA barcode groups from culture collections in this study and from GenBank as per Fig. 3, with tree labels. Barcode groups are represented by vertical lines. (TIF)
Data
Neighbour-joining phylogenetic analysis of Symbiodinium ITS DNA barcode groups from culture collections in this study and from GenBank as per Fig. 3, with tree labels. Barcode groups are represented by vertical lines. (TIF)
Data
Identification of Cultivated and Genbank deposited Dinoflagellate Strains in this Study Using COI barcodes. PWD cut-off of Species-O.T.U is 0.24% or less, Genera 0.T.U is 1.4% or less. Strain Synonyms are indicated in brackets. Identification of COI barcode are explained in the materials and methods. Cross in brackets indicates a strain is over the...
Data
Neighbor Joining Cluster Analysis of Uncorrected PWD from Culture Collections and Marine Environmental COI Barcodes as in Figure 3, with Strain Names. (0.75 MB TIF)
Data
Neighbor Joining Cluster Analysis of Uncorrected PWD from All Culture Collection COI Barcodes as in Figure 2, with Strain Names. (1.58 MB TIF)
Data
Principle Coordinate separation of DNA Barcodes from Cultured and Environmental Dinoflagellates. PCoA using the first three principle coordinates (labeled all-st-1, 2 or 3) of all environmental barcodes and culture collection strains, colour-coded in a similar manner to Fig. 3, except that cultured strains are shown in black. Most cultured strains...
Data
A comparison of dinoflagellate PWD values from four barcode studies. A: ITS, this study; B: ITS [20]; C: COB [12]; D: COI [11] and COI Prorocentrum (intraspecies only [58]). PWD were calculated by TVM_G model for COB, and uncorrected p-distances for all other barcodes. NA = data not available. Note the number of Symbiodinium taxa were recorded diff...
Data
Within group average calculations for seven species to determine presence of paralogues in COI barcodes. Five clones were sequenced per strain, except for CCMP1746 (3 clones) showing that clonal variation in all but one strain was less than 0.2%. Clones of CCMP421 revealed almost 10 times as much diversity compared to that of other dinoflagellates,...
Article
Full-text available
Background: Dinoflagellates are an ecologically important group of protists with important functions as primary producers, coral symbionts and in toxic red tides. Although widely studied, the natural diversity of dinoflagellates is not well known. DNA barcoding has been utilized successfully for many protist groups. We used this approach to system...
Article
Chlorarachniophytes are a small group of marine photosynthetic protists. They are best known as examples of an intermediate stage of secondary endosymbiosis: their plastids are derived from green algae and retain a highly reduced nucleus, called a nucleomorph, between the inner and outer pairs of membranes. Chlorarachniophytes can be challenging to...
Article
Full-text available
Matrix metalloproteinase 3 (MMP3), is over expressed in the wall of abdominal aortic aneurysms (AAA), while inactivation of the gene expressing this enzyme is associated with reduced aneurysm formation in an experimental model. The 5A allele of the 5A/6A polymorphism in the promoter region of the MMP3 gene is associated with enhanced MMP3 expressio...
Article
Fuhrmann syndrome and the Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome are considered to be distinct limb-malformation disorders characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia in humans. In families with these syndromes, we found homozygous missense mutations in the dorsoventral-patterning gene WNT7A and co...
Article
Individuals born of consanguineous union have segments of their genomes that are homozygous as a result of inheriting identical ancestral genomic segments through both parents. One consequence of this is an increased incidence of recessive disease within these sibships. Theoretical calculations predict that 6% (1/16) of the genome of a child of fir...
Article
Full-text available
The recent development of multiplex ligation-dependent probe amplification (MLPA) has provided an efficient and reliable assay for dosage screening of multiple loci in a single reaction. However, a drawback to this method is the time-consuming process of generating a probe set by cloning in single-stranded bacteriophage vectors. We have developed a...

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