Rostislav Zemek

Rostislav Zemek
Biology centre CAS České Budějovice · Biochemistry and Physiology

PhD

About

109
Publications
16,886
Reads
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1,525
Citations
Citations since 2017
36 Research Items
498 Citations
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
Additional affiliations
January 2011 - present
Institute of Entomology ASCR
Position
  • Principal Investigator
April 1995 - April 1996
University of Copenhagen
Position
  • PostDoc Position

Publications

Publications (109)
Article
Full-text available
The Colorado potato beetle, Leptinotarsa decemlineata, has developed resistance to most registered pesticides and has become one of the most difficult insect pests to control. Development of new biopesticides targeting this pest might solve the resistance problem and contribute to sustainable crop production. Laboratory experiments were conducted t...
Article
Full-text available
Entomopathogenic nematodes and fungi are globally distributed soil organisms that are frequently used as bioagents in biological control and integrated pest management. Many studies have demonstrated that the combination of biocontrol agents can increase their efficacy against target hosts. In our study, we focused on another potential benefit of t...
Article
Full-text available
The bulb mite, Rhizoglyphus robini, is a serious pest of garlic, onion and other crops. The mite is usually found in association with dangerous fungal pathogens such as Fusarium spp. Control of this pest has relied upon the use of synthetic acaricides but chemical control of the bulb mite is difficult because it is able to develop resistance quickl...
Article
Full-text available
Red raspberry, Rubus idaeus, is known to be infested by at least six species of eriophyid mites. Among them, the raspberry leaf and bud mite, Phyllocoptes gracilis, (Figure 1, A–B) is the only known vector of a raspberry virus, namely the raspberry leaf blotch virus (RLBV) (Dong et al. 2016; McGavin et al. 2012; Tan et al. 2022). Raspberry leaf blo...
Chapter
Full-text available
Mites of the genus Rhizoglyphus (Acari: Acaridae) are serious pests of plants belonging to the orders Liliales and Asparagales such as onions, garlic, lilies, and tulips. Their control by synthetic pesticides is becoming problematic as a result of resistance development in these mites and environmental and health issues. New pest control methods th...
Article
Full-text available
Mites of the genus Rhizoglyphus (Acari: Acaridae) are serious pests of plants belonging to the orders Liliales and Asparagales such as onions, garlic, lilies, and tulips. Their control by synthetic pesticides is becoming problematic as a result of resistance development in these mites and environmental and health issues. New pest control methods th...
Article
Full-text available
The horse chestnut leafminer, Cameraria ohridella, Deschka and Dimi ́c, is a moth that has invaded most of Europe since it was first recorded in Macedonia near Lake Ohrid in 1985. It attacks horse chestnut trees and causes aesthetic and vitality problems. The parasitism rate, other mortality rates, and parasitoid structure were studied during a fiv...
Patent
Full-text available
An insecticidal and acaricidal additive for a plant substrate. The essence of the technical solution is that the additive consists of a mixture of at least one strain of the entomopathogenic fungus of the genus Isaria (synonym Paecilomyces) at a concentration ranging from 10E5 to 10E7 infectious particles per 1 ml of carrier substrate and at least...
Article
Full-text available
The estimated global production of raspberry from year 2016 to 2020 averaged 846,515 tons. The most common cultivated Rubus spp. is European red raspberry (Rubus idaeus L. subsp. idaeus). Often cultivated for its high nutritional value, the red raspberry (Rubus idaeus) is susceptible to multiple viruses that lead to yield loss. These viruses are tr...
Article
Bulb crops are attacked by various soil-dwelling pests and pathogens. Entomopathogenic (EPFs) and mycoparasitic fungi (MPFs) which are distributed in natural and agricultural soils worldwide can play an important role as natural enemies of bulb pests. The species richness and density of these fungi in onion and garlic fields have not been investiga...
Article
Full-text available
The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious, widely distributed pest of potato and other crops. This pest is able to defoliate the host plant and cause severe yield loss. Moreover, the pest quickly becomes resistant to many chemical pesticides. Therefore, the development of novel biopesticides targeting this pest is ur...
Cover Page
Full-text available
https://www.mdpi.com/journal/forests/special_issues/Woody_Plants_Organisms
Article
Full-text available
The horse chestnut leaf miner Cameraria ohridella (Lepidoptera: Gracillariidae) is an invasive pest of horse chestnut Aesculus hippocastanum (Sapindales: Sapindaceae) and has spread through Europe since 1985. Horse chestnut leaf blotch is a fungal disease caused by Guignardia aesculi (Botryosphaeriales: Botryosphaeriaceae) that also seriously damag...
Article
Roztoči rodu Rhizoglyphus, tedy kořenohubové, jsou celosvětově rozšíření škůdci cibule, česneku a mnoha okrasných a jiných rostlin, proti kterým prakticky neexistují dostupné metody cílené biologické ochrany. Jedním z cílů naší studie v plném rozsahu publikované v časopise Biological Control bylo zhodnotit, jaký vliv mají aplikace entomopatogenních...
Article
Entomopatogenní hlístice čeledí Steienernematidae a Heterorhabditidae, stejně jako entomopatogenní houby řádu Hypocreales, jsou široce rozšířené půdní organismy, běžně užívané jako bioagens v ochraně rostlin. Nejedna studie již ukázala, že kombinace různých bioagens může vést ke zvýšení účinnosti proti cílovým organismům. V naší studii jsme se s oh...
Preprint
Full-text available
The horse chestnut leaf miner Cameraria ohridella (Lepidoptera: Gracillariidae) is an invasive pest of horse chestnut and has spread through Europe since 1985. The horse chestnut leaf blotch Guignardia aesculi (Botryosphaeriales: Botryosphaeriaceae) is a fungal disease that also seriously damages horse chestnut trees in Europe. The interaction betw...
Article
Full-text available
Buxus sp. is an important native and ornamental tree in Europe threatened by a serious invasive pest Cydalima perspectalis. The larvae of this moth are able to defoliate box trees and cause their death. The development of novel biopesticides targeting this pest might help protect Buxus trees grown wildly or in city parks. Laboratory experiments wer...
Conference Paper
Full-text available
The bulb mite, Rhizoglyphus robini, is serious pest of garlic, onion and many other crops. Control of this pest has relied upon the use of synthetic acaricides and insecticides, but this option is now limited mainly due to withdrawal of some chemical pesticides. Thus, alternative control methods, e. g. biological control, need to be developed and i...
Cover Page
Full-text available
https://www.mdpi.com/journal/forests/special_issues/Pest_Pathogen_Urban_Tree
Article
The adipokinetic hormones (AKHs) are known to be involved in insect immunity, thus their role in the cockroach Periplaneta americana infected with the entomopathogenic fungus Isaria fumosorosea was examined in this study. The application of I. fumosorosea resulted in a significant increase in both Akh gene expression and AKH peptide levels. Further...
Article
Bulb mites of the genus Rhizoglyphus are cosmopolitan pests of onion, garlic and ornamental plants. Despite the growing awareness against the use of pesticides, growers continue to use insecticides as the main control method. Thus, the search for environmentally safe alternative control methods, such as effective biocontrol agents, is of paramount...
Conference Paper
Full-text available
Convenient ecological alternative to broad-spectrum chemical pesticides is the utilization of natural enemies, like predators, parasitoids and microorganisms. A substantial number of microbial biopesticides based on entomopathogenic fungi have been developed worldwide since 1960s. Beauveria bassiana (Balsamo-Crivelli) Vuillemin, Metarhizium anisopl...
Article
Full-text available
The sweet cherry Prunus avium L. and sour cherry Prunus cerasus L. are considered important fruit trees, providing valuable and delicious fruit worldwide. We compared the susceptibility of 10 cultivars of sweet cherry and 5 genotypes of sour cherry to Bryobia rubrioculus Scheuten feeding by manually counting brown spots and by measuring damaged lea...
Article
Full-text available
Horse chestnut, Aesculus hippocastanum L. (Sapindaceae), is a large deciduous tree native to a small area in the Pindus Mountain mixed forests and the Balkan mixed forests of Southeast Europe. It was introduced to most countries in Europe mainly for ornamental purposes. The aim of the present study was to assess the occurrence, species composition...
Conference Paper
Full-text available
The Colorado potato beetle, Leptinotarsa decemlineata, is the most widespread and best-known insect pest that causes great economic losses especially on potatoes. As a result of the intensive use of insecticides this species has gradually developed resistance to most pesticides and its regulation is thus currently very difficult. In addition to the...
Conference Paper
Full-text available
Entomopathogenic fungus Isaria fumosorosea WIZE (Hypocreales: Cordycipitaceae) is among the most common species used in biological control. The aim of this study was to find the most suitable media for both surface and submerged productions of spores of the strain CCM 8367 of I. fumosorosea isolated in the Czech Republic from the horse chestnut lea...
Conference Paper
Full-text available
In the present study the screening of entomopathogenic nematodes in the fields of onion and garlic specialized farms in the Czech Republic and Israel was performed and more than thirty strains of Steinernema and Heterorhabditis nematodes were isolated. Selected strains were tested for infectivity and pathogenicity against garlic and onion pest mite...
Poster
Full-text available
A number of biopesticides based on entomopathogenic fungi have been developed since the 1960s. While the efficacy of these products against target pests has been documented in many studies, less is known about their effects on beneficial organisms. The objective of the present contribution is to assess non-target impacts of Isaria fumosorosea, a wo...
Conference Paper
Full-text available
This proceedings contains papers dealing with issues affecting biological control, particularly pertaining to the use of parasitoids and predators as biological control agents. This includes all approaches to biological control: conservation, augmentation, and importation of natural enemy species for the control of arthropod targets, as well as oth...
Article
Full-text available
The horse chestnut leaf miner Cameraria ohridella Deschka & Dimic (Lepidoptera: Gracillariidae) was first recorded in Europe near lake Ohrid in the former Yugoslav Republic of Macedonia in 1985 since when it has spread throughout the whole of Europe. The reasons for its spread are well documented. The aim of this study was to assess the population...
Poster
Full-text available
Bulb mites of the genus Rhizoglyphus are among the most serious pests of garlic and onion but also tulips, hyacinths and other plants. Females of these soil dwelling mites lay their eggs into the underground parts of many plants and their larvae altogether with the adult stages cause damage to plant tissues that are, consequently, more sensitive to...
Article
Full-text available
An increasing amount of attention has been devoted to studying the impact of non-native plant and animal species on native species. In this paper, we examined the antipredator response of naïve European brown frog (Rana temporaria Linnaeus, 1758) tadpoles to water-borne chemical cues from invasive red-eared slider turtles (Trachemys scripta elegans...
Conference Paper
Full-text available
Microbial biopesticides targeting the two-spotted spider mite, Tetranychus urticae, might become an alternative to broad-spectrum chemicals and thus contribute to sustainable crop production. The aim of this paper was to assess the efficacy of entomopathogenic fungus Isaria fumosorosea (syn. Paecilomyces fumosoroseus) spray applications against T....
Article
Full-text available
The ultrastructural and biochemical characteristics were studied in active and diapausing pupae of the horse chestnut leaf miner, Cameraria ohridella, collected in summer. The active pupae contained developing internal organs, while those in diapause mostly numerous non-differentiated cells packed with stored nutrients. These nutrients occurred in...
Conference Paper
Background/Question/Methods Knowledge of the flight period of insects can be of great importance to develop models that accurately forecast insect phenology. The prediction is even more crucial for species overwintering as adults, as observed in some microlepidopteran pests. Adults emerging in the spring can be expected to arise shortly after tem...
Conference Paper
Tomato leaf miner, Tuta absoluta, is an invasive pest in Europe causing serious damage mainly in greenhouses. The aim of our study was to assess the strain CCM 8367 of entomopathogenic fungus Isaria fumosorosea (syn. Paecilomyces fumosoroseus) against this pest. The strain originated from the horse chestnut leaf miner, Cameraria ohridella, and is d...
Patent
Full-text available
Strain of entomopathogenic fungus Isaria fumosorosea CCM 8367 (CCEFO.011.PFR) deposited in collection CCM (Czech Collection of Microorganisms) in Brno, applicable for biological control of substrates infested by insect and mite pests. Infested woods, plants, etc. are treated with blastospores or conidiospores of this strain.
Article
Full-text available
Laboratory experiments were conducted to assess the efficiency of the new strain CCM 8367 of Isaria fumosorosea on different stages of the Egyptian cotton leafworm, Spodoptera littoralis (Boisd.), including soil treatments with pre-pupal and pupal stages. Treatments of larval instars showed a high susceptibility with 3rd, 5th and last instars to su...
Conference Paper
Full-text available
A simple shake-and wash technique for monitoring mites was developed. A faunal survey documented twenty-five species of phytoseiids. Typhlodromus pyri was abundant only in some of commercial sprayed orchards and vineyards, or was absent. The residual leaf-dip tests indicated considerable differences in susceptibility of T. pyri strains to azinphos-...
Article
Full-text available
Isaria fumosorosea (syn. Paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. We evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of Egyptian cotton leafworm, Spodoptera littoralis. The first strain was Apopka...
Conference Paper
Full-text available
Laboratory experiments were carried out to assess the life-table parameters of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae) on leaves of four maize varieties: (1) transgenic Bt maize variety MON 88017 expressing Cry3Bb1 for control of Diabrotica spp., and protein CP4 EPSPS for a toleration of glyphosate-based herbicides,...
Presentation
Full-text available
Laboratory experiments were carried out to assess the life-table parameters of the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae) on leaves of four maize varieties: (1) transgenic Bt maize variety MON 88017 expressing Cry3Bb1 for control of Diabrotica spp., and protein CP4 EPSPS for a toleration of glyphosate-based herbicides,...
Chapter
Full-text available
The firebug Pyrrhocoris apterus (Heteroptera: Pyrrhocoridae) is a polyphage whose European populations mainly feed on the seeds of trees (Tilia cordata, T. platyphylos, Robinia pseudoacacia) or plants such as mallows (Malvaceae). It is frequently found on the ground near host trees or at the base of their trunks. During summer, however, we observed...
Article
The RP HPLC and LC/MS QTOF analyses of the methanolic CNS extract from isopod crustacean the woodlouse, Porcellio scaber revealed a presence of the red pigment-concentrating hormone (Panbo-RPCH) in this species. It has been shown that this neuropeptide plays a role in mobilization of energy stores: topical treatments of P. scaber individuals by Pan...
Article
Full-text available
An X-ray micro-radiographic system based on the Medipix2 semiconductor pixel detector for dynamic high spatial resolution and for high contrast imaging has been developed. Our system is based on a micro-focus and nano-focus X-ray tube and the hybrid single-photon counting silicon pixel detector Medipix2 (matrix 256×256 sq. pixels of 55 μm pitch). T...
Article
Full-text available
The response of Cycloneda limbifer Say (Coleoptera: Coccinellidae) larvae to conspecific larval tracks was investigated with the assistance of the automatic video tracking system EthoVision. Single larvae were monitored for 1h in an arena with a choice of two substrates, one clean and one contaminated with the tracks of conspecific larvae. Fourth i...
Article
Although the toxins of Bacillus thuringiensis Berliner (Bt) are frequently used to control lepidopteran pests, the tolerance or resistance of some lepidopteran moth strains may limit Bt applications. In this study, insecticidal cocktails consisting of sublethal doses of Cry1Ab toxin and additive compounds were tested for their suppressive effect on...
Article
In the present paper we studied the effects of five biogenic amines - norepinephrine, dopamine, octopamine, serotonin and histamine - on the locomotory activity and mobilization of lipids in the adult females of the firebug, Pyrrhocoris apterus (L.). We tested the hypothesis (1) whether the stimulation of walking activity in the bugs injected with...
Article
Full-text available
Reproducing females of the firebug, Pyrrhocoris apterus (Linnaeus) (Heteroptera: Pyrrhocoridae), reared under long-day (LD 18:6h) photocycle (long-day females), and diapausing females reared under short-day (LD 12:12h) conditions (short-day females) were analysed for temporal patterns of feeding, drinking and gut digestive enzyme activities. In lon...
Article
Full-text available
The role of herbivore-induced volatile substances in prey-finding by phytoseiid mites has been repeatedly documented using an olfactometer. The objective of the present paper is to test the hypothesis that movement by Phytoseiulus persimilis is affected by these volatiles even on plants. Two series of laboratory experiments were carried out. In the...
Article
Full-text available
The aerial transport of tiny arthropods including wing-less species has been well documented. The initiation of aerial dispersal in most instances is not a passive phenomenon, but involves identifiable behaviors in response to specific stimuli. Here we describe a construction of wind tunnel suitable for close-up observation of micro-arthropods beha...
Article
Full-text available
In the present study we tested whether the walking activity of macropterous females of the flightless wing-polymorphic bug Pyrrhocoris apterus (L.) can be stimulated by its native adipokinetic hormone Peram-CAH-II and the crustacean red pigment-concentrating hormone (Panbo-RPCH), and the effectiveness of the latter hormone im a lipid mobilization a...
Article
Current theory on trait-mediated interactions in tri-trophic food chains shows that antipredator behaviour of the middle species can cause similar indirect effects giving rise to trophic cascades as mediated by den- sity changes. In this article, the effect of predation risk in a tri-trophic food chain (bean plantetwo-spotted spider mite preyepreda...
Article
Full-text available
The horse chestnut leaf-miner, Cameraria ohridella Deschka et Dimic (Lepidoptera, Gracillariidae) is a serious invasive pest of Aesculus hippocastanum in Europe. The larvae of this species feed on leaf parenchyma and can reduce the tree growth. We studied the impact of parasitoids on C. ohridella in the Czech Republic and also searched for entomopa...
Article
Full-text available
The influence of female and male cues on the male searching behavior of the sheep tick, Ixodes ricinus (Acari: Ixodidae), was studied under constant laboratory conditions by means of a computerized video tracking system. Males were released into an open glass arena in which one half had been exposed for 1 h to 5 freely walking unfed females or five...
Article
Full-text available
The article describes a new method to assess the impact of the two-spotted spider mite, Tetranychus urticae Koch, on host plants. The method is based on measurement of the leaf area damaged using a computerized image analysis technique. Injured leaves were scanned on a common flat bed color scanner to obtain grey scale images. MATHEMATICA software...
Article
Full-text available
Abstract The present study tested whether the pattern of feeding activity in the firebug Pyrrhocoris apterus (L.) is sex- and wing morph-related, diurnal or nocturnal, as well as whether the feeding rhythm persists in constant darkness. Temporal patterns of feeding activity are analysed in macropterous and brachypterous adults reared under long-day...
Article
Full-text available
The effect of snowdrop lectin (Galanthus nivalis agglutinin: GNA) expression on host-plant preference was examined with a two-choice disc test. Discrimination between a transgenic potato [Solanum tuberosum (Solanaceae)] capable of synthesizing GNA, and an isogenic cultivar, was studied under laboratory conditions using the glasshouse potato aphidAu...
Article
Full-text available
  Prey consumption by Typhlodromus pyri Scheuten was studied in the presence and absence of apple powdery mildew, Podosphaera leucotricha (Ell. and Everh.) under constant laboratory conditions. Eggs of Tetranychus urticae Koch were offered to predatory mites as a prey. Seven densities ranging from five to 100 T. urticae eggs per arena were used. Mi...
Article
Full-text available
The behavior of the two-spotted spider mite, Tetranychus urticae Koch and the predatory mite Phytoseiulus persimilis A.-H. was investigated in laboratory experiments with transgenic Bt-eggplants, Solanum melongena L., producing the Cry3Bb toxin and corresponding isogenic, non-transformed eggplants. In bitrophic experiments, dual-choice disc tests w...