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April 1988 - September 2021
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Publications (146)
Ethnopharmacological relevance:
Herbal medicinal products based on ginkgo leaf refined dry extract (GBE) are an European development from the Eastern Asia traditionally used species Ginkgo biloba L. Nowadays, ginkgo products have increased the presence in the market, mainly as dietary supplements. Its adulteration with rutin and quercetin or herba...
In Valencia Region (Spain), some wild and cultivated sages are used for medicinal purposes. Among them, Salvia officinalis subsp. lavandulifolia (SL) is widely employed and known for production of Spanish sage oil and herbal products. Nevertheless, it shares the market with S. blancoana subsp. mariolensis (SB) and, to a lesser extent, with their hy...
The chemical composition of leaf essential oils from 11 species of Piper from Panama was analyzed by a combination GC-FID and GC-MS procedures. Six of them had sesquiterpene hydrocarbons as major constituents, three were characterized by monoterpene hydrocarbons, one by a diterpene, and one by a phenylpropanoid, dillapiole. The main components iden...
Fifteen essential oils, four essential oil fractions and three pure compounds (thymol, carvacrol and eugenol), characterized by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), were investigated for biological and non-biological antioxidant activity. Clove oil and eugenol showed strong DPPH (2,2-diphenyl-1-picrylhydrazyl) f...
The chemical composition of the leaf essential oils obtained by hydrodistillation of 6 Piper sp.(Piperaceae) from Panama were analyzed by a combination of GC-FID and GC-MS using two capillary columns of different stationary phases (Supelcowax™10 and methylsilicone SE-30). Identification of constituents was achieved by means of their GC retention in...
Objectives
This study aimed to evaluate the possible antioxidant activity of Tween-20 and Tween-80, two amphipathic nonionic surfactants commonly used as solubilizers and stabilizers, whose pharmacological effects have been ignored to a large degree.Methods
Antioxidant activity was investigated in vitro measuring the scavenging activity on the stab...
The aim of the present study was to investigate the in vitro activity of fifteen essential oils, four essential oil fractions and three pure compounds (thymol, carvacrol and eugenol) on phagocytosis by human neutrophils and on complement system. Samples were characterized by GC and GC-MS. Most of the oils (nutmeg, clove, niaouli, tea tree, bay laur...
A single dose of eugenol dissolved in refined sunflower oil, was administered by oral gavage, at a dose of 1.5 mmol/Kg (246 mg/Kg) to the treated group (n=3) of chickens. Two control groups (n=3) were included in the experiment, one without any treatment and the other administered with refined sunflower oil. Faeces were collected at 4, 24, 29 and 4...
We are most grateful to the authors for their generous and timely response in spite of their research and academic responsibilities.
Due to the increase of the incidence of fungal infections in humans and the limitations of the available antimycotic drugs, among which the emergence of resistant strains, there is a need for the discovery of new antifungal agents. Plants, especially those used in Traditional Medicine, linked to an unmatched chemical diversity, either as pure compo...
The antifungal activity of hexane, dichloromethane, methanol and aqueous extracts from the rhizome and root of Ferula hermonis was assayed in vitro by the agar disk diffusion method against a panel of human opportunistic and pathogenic fungi. Among them, the hexane and dichloromethane extracts showed the highest activity particularly against the de...
The essential oil from the leaves of Siparuna thecaphora was analyzed by GC and GC/MS. Forty-six components were identified representing approximately 60% of the oil, among which oxygenated sesquiterpenes (27%), sesquiterpene hydrocarbons (18%) and linear oxygenated compounds (11%) were the major groups of constituents, with spathulenol 9.4%), 2-tr...
Myeloperoxidase (MPO) is a key component of innate immune defence, linked to inflammatory diseases. In the present
work, the effect on MPO activity has been studied for 15 essential oils (EO) (bay laurel, cajeput, clove, coriander, ginger,
juniper, lemon, lemon grass, niaouli, nutmeg, rosemary, red thyme, Spanish oregano, tarragon and tea tree) and...
Peak overlap is an important cause of erroneous quantification in HPTLC, and color differences of zones are not usually studied. A new adaptive algorithm was developed based on the assumption that the zone due to each component resembles the shape of a gaussian distribution. The chromatogram is the sum of the different gaussian peaks, and the algor...
Ziziphora aragonensis Pau and Micromeria fruticosa (L.) Ducre (Lamiaceae) are usually grown in Spanish home gardens for medicinal purposes. They are commonly known as "poleos" and traditionally used as teas for gastrointestinal disorders and colds. While the chemical composition of the essential oil of M. fruticosa has been widely analyzed, there a...
The essential oils from aerial parts of nine populations of Zuccagnia punctata Cav. (Fabaceae) were obtained by hydrodistillation and analyzed by GC–FID, GC–MS and 13C NMR. A total of 80 constituents, mainly oxygenated monoterpenes, were identified representing from 79.0 to 95.2% of the total oils which showed different composition patterns. The un...
This contribution was funded by a grant obtained in 2010 from the Institute for Catalan Studies for the study ‘Chemical characterization of the main sages used as a spice or for medicinal purposes in the Valencia region.’ The authors are grateful to the Institute for Catalan Studies for financial support through the Borsa d’estudi Paisos Catalans....
In this study, the antiprotozoal activity of the sesquiterpene lactone psilostachyin C was investigated. This natural compound was isolated from Ambrosia scabra by bioassay-guided fractionation and was identified by spectroscopic techniques. Psilostachyin C exerted in vitro trypanocidal activity against Trypanosoma cruzi epimastigotes, trypomastigo...
Ivy leaf is an herbal drug used since ancient times for medicinal purposes. Its extracts are widely used in the treatment of respiratory conditions, mainly due to its expectorant and spasmolytic activities. The active principles are pentacyclic triterpene saponins, the major one being hederacoside C, a bidesmosidic glycoside that in the body is tra...
In a previous work, the essential oil from the dried root of Ferula hermonis Boiss. (Umbelliferae), whose major constituents were alpha-pinene (43.3%), alpha-bisabolol (11.1%) and the unusual acetylene 3,5-nonadiyne (4.4%), showed moderate antifungal activity against Trichophyton mentagrophytes [1]. With the aim of characterizing the active constit...
Acalypha guatemalensis Pax & Hoffm. (Euphorbiaceae), a plant native from Guatemala and Honduras, is used in folk medicine to treat protozoal infections, gastrointestinal and venereal diseases, rheumatism, cancer and for several other purposes [1]. In a previous work, the dichloromethane extract from dried leaves showed an inhibitory effect on class...
The essential oil from fresh leaves of Plinia cerrocampanensis Barrie (Myrtaceae), obtained by hydrodistillation, was analysed by GC-FID and GC-MS. Forty components, representing more than 91% of the oil, were identified. Oxygenated sesquiterpenes represented the main fraction with alpha-bisabolol (42.8%) as the major constituent, making this plant...
The composition and biological activity of the essential oils from leaves, fruits, stems and bark of Protium confusum are reported for the first time. Forty-six to sixty-three constituents were identified ranging from 73.8% to 98.5% of the samples.
Limonene (60.2%) was the main component in the fruit oil, whereas spathulenol (19.3%), β-caryophyllen...
Cordia verbenacea DC (Borraginaceae) is a native plant of the Brazilian coasts, used in folk medicine to treat arthritis, wounds and contusions.The essential oil of the leaves has shown anti-inflammatory and anti-allergic activities, and it is used in a topically applied herbal medicinal product (Acheflan®) for the treatment of chronic tendonitis,...
Lepechinia meyenii (Walp.) Epling (Lamiaceae), locally known as „pacha salvia“ or „puna salvia“, is a stoloniferous perennial herb which grows in high altitudes in Peru, Bolivia and Argentina. In the communities from the high Andes of Peru (Ayacucho), it is used to treat respiratory ailments, cough and bacterial infections. It is also used to flavo...
Nitric oxide (NO) plays a key role in the production of reactive nitrogen species (RNS), which have cytotoxic properties against pathogenic microbes and, at the same time, can damage host tissues [1]. Some essential oils have antioxidant properties and their consumption can influence immune cell functions [2,3]. In order to widen our knowledge of t...
Bioassay-guided fractionation of the organic extract of Ambrosia tenuifolia Sprengel (Asteraceae) led to the isolation of two bioactive sesquiterpene lactones with significant trypanocidal and leishmanicidal
activities. By spectroscopic methods (1H- and 13C-nuclear magnetic resonance, distortionless enhancement by polarization transfer, correlated...
The analysis of the essential oil from rhizome and roots of Ferula hermonis Boiss. (Apiaceae) by GC–FID, GC–MS and 13C NMR allowed the identification of 79 constituents, more than 90% of the oil, the major one being α-pinene (43.3%), followed by α-bisabolol (11.1%) and the unusual acetylenic compound 3,5-nonadiyne (4.4%).
The antifungal activity o...
The tea tree oil is the essential oil obtained from the leaves and twinglets of Melaleuca alternifolia. Its major constituent is terpinen-4-ol. This compound is considered the main responsible, together with other constituents, such as α-terpinene, γ-terpinene and α-terpineol, of the antibacterial, antifungal, antiprotozoary against trichomonas, an...
The essential oil from leaves and twigs of Luma chequen from Peru, obtained by steam distillation, was analysed by a combination of GC–FID, GC–MS and 13C-NMR. Forty constituents (93.6% of the total sample) were identified. Monoterpenes constituted the main fraction (87.7%), the major ones being α-pinene (57.3%) and 1,8-cineole (7.5%), whereas sesqu...
The essential oil from leaves of Lippia chiapasensis obtained by hydrodistillation was analyzed by GC and GC/MS. From the MS data and retention indices, 96.0% of the oil consisting of 142 components could be identified. The essential oil was characterized by a high percentage of oxygenated monoterpenes (mainly carbonylic), the most important being...
Ferula hermonis Boiss. (Umbelliferae) grows above 6000 feet in the mountains between Lebanon and Syria. Its root, locally known as Zallouh root, is used in infusion as an aphrodisiac as it enhances male sexual behaviour. In order to search for new antifungal agents, in this work we assayed the antifungal activity of its extracts.
The essential oil obtained by hydrodistillation from the leaves of Litsea guatemalensis from Guatemala was analysed by GC–FID and GC–MS; 74 constituents (97.3% of the total sample) were identified. Oxygenated monoterpenoids constituted the main fraction (72.2%), the major ones being 1,8-cineole (26.8%), α-terpineol (14.5%) and linalool (10.8%), whe...
The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC–MS and 13C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), β-elemene (20.0%), β-caryophyllene (8.8%) and β-bou...
The essential oil of Ageratum conyzoides from S. Tomé and Príncipe was investigated for the first time. The oil was obtained by hydrodistillation of the aerial parts, and subsequently analyzed by GC, GC/MS and C-NMR. The oil was characterized by the presence of high percentages of precocene I (34.4%) and β-caryophyllene (24.6%), as well as small am...
The bioassay-guided fractionation of the antifungal dichloromethane extract from the roots of Vernonanthura tweedieana (Baker) H. Rob. (Asteraceae), using an agar overlay bioautographic method, allowed the isolation of one active sesquiterpene (1), identified as 6-cinnamoyloxy-1-hydroxyeudesm-4-en-3-one. Minimal inhibitory concentrations (MIC) and...
The chemical compositions of the essential oils from the leaves of Piper aduncum from Panama and Bolivia were investigated by GC-FID, GC-MS and 13C-NMR. In total, 55 components were identified representing 99.3 and 80.3% of each sample, respectively. The oil from Panama was characterized by a high percentage of sesquiterpene hydrocarbons (38.9%), th...
La Fitoterapia es una parte de la terapéutica, cuyo desarrollo racional requiere disponer de medicamentos a base de plantas, cuya calidad, seguridad y eficacia estén garantizadas, teniendo en cuenta las especiales características de las drogas vegetales y extractos. La calidad, que es el parámetro en que se basa la reproducibilidad de la seguridad...
The essential oil of aerial parts of Salvia guaranitica, grown in a small-scale experimental field in Uruguay, was analysed by GC, GC–MS and 13 C-NMR; 49 constituents (86.6% of the total sample) were identified. The major components were found to be sesquiterpene hydrocarbons: germacrene D (25.0%), β β β β β-elemene (20.0%), β β β β β-caryophyllene...
Phytotherapy is a part of Therapeutics, the rational development of which needs herbal medicinal products (HMP) with guaranteed quality, safety ad efficacy, taking in account the special characteristics of herbal drugs and extracts. Quality is the basis for the reproducibility of safety and efficacy, and it must be controlled since the early steps...
The qualitative and quantitative composition of the essential oil from aerial parts of Eupatorium buniifolium Hooker et Arnott (Asteraceae) has been investigated for the first time. The essential oils were obtained by steam distillation of leaves and analysed by GC-MS; 44 components were identified. Monoterpene and sesquiterpene hydrocarbons, espec...
The chemical composition of the essential oils from leaves and stems of Aristolochia gibertii Hooker collected in Paraguay was investigated by GC and GC/MS. The composition of both oils consisted mainly of sesquiterpenes and a small percentage of monoterpenes; 46 constituents being identified in total. The major component was bicyclogermacrene (26....
A biotype of clary sage (Salvia sclarea L.) growing in Uruguay was evaluated for its suitability to be cultivated in Uruguay. Essential oils from the inflorescences and leaves of plant harvested at full flowering and early seed ripeness stages were obtained by steam distillation and characterized by GC and GC–MS (quadrupole). Twenty-seven components...
The essential oil from leaves of Eugenia acapulcensis Steud. obtained by hydrodistillation was analyzed by GC and GC/MS. From the MS data and retention indices obtained 56.7% of the oil consisting of 47 components could be identified. Eighty-five percent of the analyzed oil was comprised of sesquiterpenes, either hydrocarbons or oxygenated, among w...
The composition of the essential oil from leaves of Lippia myriocephala Schltdl. et Cham. from Costa Rica was analyzed by capillary GC/FID and GC/MS. Fifty-nine compounds were identified corresponding to about 92% of the oil. The major components were β-caryophyllene (16.1%), germacrene D (11.2%), β-cubebene (8.1%), geranylacetone (7.3%) and α-copa...
The composition of the essential oils from leaves of Lepechinia mutica, Satureja glabrata, Minthostachys mollis and Myrcianthes rhopaloides, four species used in traditional medicine in Loja (Ecuador), was investigated by GC–FID and GC–MS. Monoterpene hydrocarbons were the main group of constituents in the essential oil of L. mutica, especially β-p...
The essential oil of Cedrela odorata L. bark from S. Tomé and Príncipe, where this plant is used in traditional medicine mainly for the treatment of malaria as well as a febrifuge and against diabetes, was investigated for the first time. The oil was obtained by hydrodistillation of the bark, and subsequently analyzed by GC, GC/MS and C-NMR. Thirty...
The immunomodulatory activity of the latex from Croton lechleri (sangre de drago) was determined by in vitro assays. Classical (CP) and alternative (AP) complement pathways activities were determined in human serum. Intracellular generation of reactive oxygen species (ROS) by human polymorphonuclear leukocytes (PMNs) and monocytes, and phagocytosis...
Essential oils obtained by hydrodistillation from the leaves and flower spikes of Piper friedrichsthalii from Costa Rica and Panama, and leaves of P. pseudolindenii from Costa Rica were analysed by GC–FID, GC–MS and 13C-NMR. Monoterpenoids constituted the main fraction of the oils of P. friedrichsthalii from Costa Rica, particularly α-pinene, camphe...
The essential oil of Eryngium foetidum leaves from S. Tomé e Príncipe was investigated for the first time. The oils were obtained by hydrodistillation of the leaves, from two different sites, and subsequently analyzed by GC, GC/MS and C-NMR. The oils were characterized by the presence of small amounts of monoterpene hydrocarbons and sesquiterpenoid...
The composition and the antimicrobial activity of the bark oil of Santiria trimera (Oliv.) Aubrév., a plant widely used by the traditional healers in S. Tomé and Príncipe, especially for wound healing, are reported for the first time. The analysis of the essential oil was carried out by GC and GC-MS. The oil contains a high content of monoterpenes,...
Essential oils obtained by hydrodistillation from leaves of Mentha pulegium L. and Mentha rotundifolia (L.) Huds. from Uruguay were analysed by GC-FID and GC-MS. Oxygen-containing monoterpenes were the main group of constituents in both oils. Pulegone, isomenthone and menthone were the major components in the oil of M. pulegium, whereas piperitenon...
A fucoarabinogalactan (CU-1), the main component of the gum exudate of the medicinal plant Croton urucurana, has been isolated by precipitation and subsequent dialysis and finally purified by ion-exchange chromatography. The estimated average molecular weight of CU-1 by gel permeation chromatography was approximately 2.48 x 10(6) Da. CU-1 was found...
The composition of the essential oils from leaves and seeds ofBlepharocalyx tweediei and B. gigantea, two closely related taxa from Argentina, was determined by capillary GC, GC/MS and C-NMR. The main compounds in the oils of B. tweediei were α-and β-pinene, 1,8-cineole and limonene, linalool being also a major constituent of the oil from leaves. T...
Volatile constituents of the essential oils from leaves and inflorescences of Solidago chilensis Meyen were analyzed by GC-FID, GC-MS and 13C-NMR and thirty-six different compounds were identified. Pumiloxide, an unusual labdane diterpene, was found to be one of the major components in both oils (15.3 % and 12.3 %, respectively). Other important co...
The essential oils obtained from the aerial parts of Origanum scabrum and Origaum microphyllum, both endemic species in Greece, were analyzed by means of GC and GC-MS. Forty-eight constituents were identified, representing 98.59 and 98.66% of the oils, respectively. Carvacrol, terpinen-4-ol, linalool, sabinene,R-terpinene, andγ-terpinene were found...
The composition of the essential oil from leaves of Zanthoxylum procerum Donn. Sm. from Costa Rica, was analyzed by capillary GC/FID and GC/MS. Seventy-eight compounds were identified corresponding to ca. 97% of the oil. The major components were the monoterpenoids β-phellandrene (30.0%), α-pinene (8.5%), myrcene (7.1%), α-phellandrene (6.1%), limo...
Activity-guided fractionation of the dichloromethane extract from leaves of Piper fulvescens, using an agar overlay bioautographic method, led to the isolation of three antifungal neolignans identified as conocarpan, eupomatenoid 5 and eupomatenoid 6. The minimal inhibitory concentration of these three neolignans against five fungi strains were det...
The essential oil composition of three Zingiberaceae widely used as medicinal aromatic plants from S. Tomé and Príncipe: Aframomum danielli (Hook. f.) K. Schum., Curcuma longa L. and Zingiber officinale Rosc. was studied. Two samples of the essential oils from fruit of A. danielli and from rhizomes of the other two species, were obtained by hydrodi...
The essential oil of aerial parts of Lippia alba (Mill.) N. E. Brown cultivated in a small-scale experimental area was analysed by GC and GC–MS (quadrupole). Twenty-seven components were identified, corresponding to 93% of the total oil. The enantiomeric distribution of α-pinene, sabinene, limonene and linalool was studied by multidimensional GC–GC...
Essential oils obtained by hydrodistillation from leaves and spikes of Piper lanceaefolium H.B.K. of Costa Rica were analysed by GC-FID, GC-MS and 13C-NMR methods. Main constituents found in the oil from leaves were sesquiterpene hydrocarbons — especially β-caryophyllene and germacrene D — and phenylpropanoids, of which elemicin and parsley apiol w...
The antifungal activity of aqueous, dichloromethane and methanol extracts from 14 Paraguayan plants used in traditional medicine for the treatment of skin diseases was assayed in vitro by the agar disk diffusion method against 11 fungal strains comprising several filamentous fungi and yeasts. Among them, the dichloromethane extracts of Acanthosperm...
The essential oil from the leaves of the aromatic aniseed smelling plant Piper fulvescens, obtained by hydrodistillation, was investigated by GC and GC-MS, as well as by 13C-NMR after fractionation of the oil by column chromatography. A total of 37 constituents, representing 78.2% of the oil were identified. The main compounds were trans-anethole (...
The composition and the antimicrobial activity of the bark oil of Croton stellulifer, an endemic and rare species of these islands (S. Tomé and Príncipe) are reported. Analysis was carried out by GC, GC/MS and 13C-NMR. The major constituents were alpha-phellandrene (15.4-18.6%), p-cymene (14.4-17.7%), linalool (12.0-12.6%) and alpha-pinene (8.1-9.1...
The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed...
The composition of the essential oils of four populations of Thymus villosus subsp. lusitanicus (Boiss.) Coutinho from Portugal was investigated by GC and GC-MS. To study the chemical polymorphism the results obtained from GC analyses of the volatile oils from individual plants from four populations were submited to Principal Component and Cluster...
The composition of the essential oils of four populations of Thymus lotocephalus G. López
and R. Morales and one population of T. x mourae Paiva and Salgueiro, two endemic taxa from Portugal, was investigated mainly by GC and GC-MS. T. x mourae is a natural hybrid between T. lotocephalus and T. mastichina (L.) L. subsp. donyanae R. Morales, which e...
Essential oils obtained by hydrodistillation from leaves and fruits of Hedyosmum mexicanum Cordemoy, leaves of H. bonplandianum Kunth and leaves of H. costaricensis C.E. Wood from Costa Rica were analyzed by GC-FID and GC-MS. GC-IR and 13C-NMR analysis were performed to improve the identification of some compounds. Monoterpene hydrocarbons, especia...
The composition of essential oil of Satureja viminea L. was analyzed by GC and GC/MS. More than 40 compounds were identified corresponding to ca. 94% of the oil. The major components were p-menth-3-en-8-ol (40.0%), pulegone (35.3%) and p-mentha-3,8-diene (5.2%).
Micropropagation of L. dentata L. through shoot-tips (ca. 5 mm) was achieved successfully. Micropropagated plantlets were cultured without plant regulators
in the culture medium (control) or in media containing 0.1 mg l−1 of 6-benzyladenine (BA) and/or indole-3-butyric acid (IBA). These plantlets were examined for their essential oil content
and co...
The composition and the antimicrobial activity of the essential oil from the leaves of Peumus boldus is investigated. Analyses of the oil obtained by hydrodistillation were carried out by GC and GC-MS using columns of two different stationary phases. Fractionation of the essential oil by column chromatography on silica gel was performed to improve...
The essential oils of OCIMUM CANUM, O. GRATISSIMUM and O. MINIMUM grown in S. Tomé, where they are used in Traditional Medicine mainly as febrifuge and for the treatment of respiratory diseases, were investigated. The essential oils were obtained from the aerial parts by hydrodistillation and subsequently analysed by GC, GC-MS and (13)C-NMR. (13)C-...
Aqueous, ethanol and chloroform extracts from Corrigiliola telephiifolia, Echinops spinosus, Kundmannia sicula, Tamarindus indica and Zygophyllum gaetulum were evaluated for antiinflammatory properties in mice (ear oedema induced by arachidonic acid) and rats (subplantar oedema induced by carrageenan) after topical or i.p. administration, respectiv...
The essential oils from Piper capense, P. nigrum, P. guineense and P. umbellatum from S. Tomé e Prı́ncipe were investigated for the first time. They were analysed by GC, GC-mass spectrometry and 13C NMR. Monoterpene hydrocarbons were the main group of constituents in three of the samples (P. capense, P. nigrum and P. umbellatum), whereas for the ot...
Dichloromethane and methanol extracts of 19 Latin American plants, most of them selected on the basis of traditional medicine reports, were subjected to screening for antifungal activity, using the agar disk diffusion assay against several fungi. Of the extracts tested, those of Andira inermis, Andira surinamensis, Bixa orellana, Blepharocalyx twee...
Aqueous (AE), ethanol (EE) and dichloromethane (DE) extracts from the whole plant of Anthurium cerrocampanense Croat, endemic to Panama, were assayed for antiinflammatory activity by carrageenan-induced rat paw edema and croton oil-induced mouse ear edema tests. The extracts were administered i.p. and topically, respectively. The best antiinflammat...
The chemical composition of the leaf essential oils of Piper arboreum, P. fimbriulatum and P. obliquum were analyzed by a combination of GC-FID, GC-mass spectrometry and 13C NMR methods. The main constituents found in the oil of P. arboreum were δ-cadinene, α-copaene and β-pinene; in the oil of P. fimbriulatum, β-caryophyllene and the oxygenated mo...
The volatile oils from roots, stems and leaves of Aristolochia elegans Mast., collected at different stages of development, were obtained by hydrodistillation and analysed by GC and GC-mass spectrometry. All the samples of essential oils were found to be rich in sesquiterpenes. Sesquiterpene hydrocarbons, in particular β-caryophyllene, isocaryophyl...
The composition and chemical polymorphism of the essential oils of several populations of Thymus mastichina subsp. mastichina, T. mastichina subsp. donyanae and T. albicans, which belong to the Section
Mastichina of the genus Thymus, were investigated by GC and GC-MS. AII representative population samples of these three taxa were characterized by t...
The composition and variability of the essential oils of several populations of Thymus caespititius from Portugal were investigated by GC, GC-mass spectrometry and 13C NMR. All samples from NW Portugal were characterized by their high α-terpineol content, while the main components in the oil sample from Pico island (Azores archipelago) were carvacr...
The composition of the essential oils of seven populations of Thymus camphoratus from Portugal and their infraspecific variability were investigated by GC, GC-mass spectrometry and 13C NMR. The results obtained from GC analyses of the volatile oils from individual plants were submitted to Principal Component and Chemometric Cluster analyses. 1,8-Ci...
The composition of the essential oils of six populations of Thymus villosus L. ssp. villosus, an endemic species of Portugal belonging to the section Pseudothymbra Bentham of the genus Thymus (Lamiaceae), was investigated mainly by GC and GC-MS. In order to study the infraspecific variability, the results obtained by the analysis of individual plan...
The chemical composition of the essential oil of Aristolochia melanoglossa Speg. from Argentina was investigated by GC and GC/MS. The oil consisted mainly of sesquiterpenes and a small percentage of monoterpenes. A total of 26 compounds were identified. The major components found in the oil were spathulenol (17.75%), β-caryophyllene (17.70%) and ca...
The leaf oils of Satureja odora (Gris.) Epl. And S. parvifolia (Phil.) Epl. (Lamiaceae), two endemic species of Argentina, were investigated by GC and GC/MS. Both species showed great difference in their composition, the main compounds being pulegone (61.5%) in S. odora and carvacrol (34.0%), carvacryl acetate (14.7%), p-cymene (14.0%) and γ-terpin...
The essential oils of two samples of Satureja boliviana (Benth.) Briq. collected in Peru and Bolivia, were obtained by hydrodistillation, and analyzed by capillary GC and GC/MS. The major constituents of the Peruvian oil were isomenthone (29.0%), pulegone (12.6%) and menthone (12.6%). Main constituents of the Bolivian oil were isomenthone (27.0%),...