Rosario Linacero

• PhD
• Professor at Complutense University of Madrid

The labelled of pistachio on food products is mandatory and, as a consequence, the development of suitable analytical methodologies to detect this nut in processed foods is advisable. In this work, two different qPCR assays to detect pistachio, SYBR®Green and locked nucleic acid (LNA) probes, are tested and compared. Pis v allergen coding sequences...

Food processing can alter protein biochemical properties, impacting immunoreactivity and allergenicity. A key feature of food allergens is their resistance to enzymatic digestion, particularly by pepsin and trypsin. This study compares the digestomes of raw and heat- and/or pressure-treated peanuts, hazelnuts, pistachios and cashews using the INFOG...

Food processing can modify protein biochemical properties altering its immunoreactivity and allergenicity. Resistance to enzymatic digestion is one of the main characteristics of food allergens, specially to pepsin and trypsin. In this work, the aim is to compare the digestomes of raw and heat and /or pressured treated peanuts, hazelnut, pistachio...

This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridiz...

Understanding the bacteria associated with nitrification and denitrification is crucial for comprehending the processes that lead to nitrous oxide emissions in agricultural greenhouse soils. Therefore, it is important to determine their abundance and expression to gain insight into these processes. The aim of this study was to explore the bacterial...

Understanding the denitrification process in agriculture is key to preventing nitrogen (N) losses and reducing emissions of the greenhouse gas nitrous oxide (N2O) to the atmosphere. Since the type of fertilization influences this process, in this study we evaluated the comparative effect of conventional (CON) versus ecological intensification (EI)...

Plant tissue culture is a source of genetic changes that are referred to as somaclonal variation. Somaclonal variation as a cause of variability has advantages and disadvantages. It is undesirable when in vitro culture is used as a microprogation tool to clone genetically identical plants. However, as a source of new intraspecific variation, it can...

Epigenetic changes induced in in vitro culture affect gene expression in a more or less permanent way, and in some cases are transmitted to the progeny. These changes affect chromatin structure and include changes in DNA methylation, histone modifications and the expression of small RNAs involved in DNA methylation (RNA-directed DNA methylation; Rd...

Hazelnut, pistachio and cashew are tree nuts with health benefits but also with allergenic properties being prevalent food allergens in Europe. The allergic characteristics of these tree nuts after processing combining heat, pressure and enzymatic digestion were analyzed through in vitro (Western blot and ELISA) and in vivo test (Prick‐Prick). In t...

Among food allergens, peanut is one of the most critical. This study evaluates peanut allergenic features after the combination of heat, pressure, and enzymatic digestion under sonication, by immunodetection using serum IgE of sensitized patients and mass-spectroscopy. In the studied population, there was a predominance of patients with sensitizati...

Nuts are considered healthy foods due to their high content of nutritional compounds with functional properties. However, the list of the most allergenic foods includes tree nuts, and their presence must be indicated on food labels. Most nut allergens are seed storage proteins, pathogenesis-related (PR) proteins, profilins and lipid transfer protei...

Peanut (Arachis hypogaea) contains allergenic proteins, which make it harmful to the sensitised population. The presence of peanut in foods must be indicated on label, to prevent accidental consumption by allergic population. In this work, we use chloroplast markers for specific detection of peanut by real-time PCR (Polymerase Chain Reaction), in o...

Peanut (Arachis hypogaea) contains allergenic proteins, which make it harmful to the sensitised population. The presence of peanut in foods must be indicated on label, to prevent accidental consumption by allergic population.. In this work, we use chloroplast markers for specifically detection of peanut by real-time PCR, in order to increase the as...

Nuts confer many health benefits due to of their high content of vitamins and 10 antioxidants and they are increasingly consumed in the last years. Currently, tree nuts are included 11 in the list with the most allergenic ingredients, and its presence must be indicated in label foods. 12 Most nut allergens are seed storage proteins, lipid transfer...

Peanut (Arachis hypogaea) contains allergenic proteins, which make it harmful to the sensitive population. The presence of peanut in foods must be indicated on label, to prevent accidental consumption by allergenic population. The development of suitable analytical methodologies to detect this allergen in processed foods is advisable. Real Time PCR...

Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-Time P...

Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitised population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-time P...

Tree nuts show nutritional properties and human health benefits. However, they contain allergenic proteins, which make them harmful to the sensitive population. The presence of tree nuts on food labelling is mandatory and, consequently, the development of suitable analytical methodologies to detect nuts in processed foods is advisable. Real-time PC...

Pistachio and cashew contain allergenic proteins, which causes them to be removed from the diet of allergic people. Previous studies have demonstrated that food processing (thermal and non-thermal) can produce structural and/or conformational changes in proteins by altering their allergenic capacity. In this study, the influence of instant controll...

Pistachio and cashew contain allergenic proteins, which causes them to be removed from the diet of allergic people. Former evidences have demonstrated that food processing (thermal and non-thermal) can produce structural and/or conformational changes in proteins by altering their allergenic capacity. In this study, the influence of Instant Controll...

Tree nuts confer many health benefits due to their high content of vitamins and antioxidants, and they are increasingly consumed in the last few years. Food processing is an important industrial tool to modify allergenic properties of foods, in addition to ensuring safety and enhancing organoleptic characteristics. The effect of high pressure, with...

Chestnut is gaining importance as food allergen, but specific detection method has not been performed so far. For the first time, the development of a real-time PCR assay for chestnut allergen detection in processed foodstuff is described. Initially, three sets of novel primers specific for allergen-encoding genes (Cas s 5, Cas s 9 and Cas s TLP) w...

Pistachio and cashew contain allergenic proteins, which causes them to be removed from the diet of allergic people. Former evidences have demonstrated that food processing (thermal and non-thermal) can produce structural and/or conformational changes in proteins by altering their allergenic capacity. In this study, the influence of Instant Controll...

Nuts are a rich source of nutritional compounds with beneficial effect on health. The objective of this study was to evaluate the effect of heat and/or pressure processing on protein and phenolic compounds (anthocyanins, flavonols, tartaric esters and total phenols) content, antioxidant activity and functional properties of pistachio, cashew and ch...

Currently, food allergies are an important health concern worldwide. The presence of undeclared allergenic ingredients or the presence of traces of allergens due to accidental contamination during food processing poses a great health risk to sensitized individuals. Therefore, reliable analytical methods are required to detect and identify allergeni...

Predicting the coping responses of tree species to climate change is, in many cases, limited by a lack of fundamental genetic insights, such as detailed knowledge of their genomes. Relict tree species may serve as models to assess the general mechanisms and patterns of forest tree responses to the changing climate and to explore the adaptive capaci...

Thermal processing can modify the structure and function of food proteins and may alter their allergenicity. This work aimed to elucidate the influence of moist thermal treatments on the IgE-reactivity of cashew and pistachio. IgE-western blot and IgE-ELISA were complemented by Skin Prick Testing (SPT) and mediator release assay to determine the Ig...

Cashew (Anacardium occidentale) nut can trigger serious reactions in allergic patients, including anaphylaxis and death. Labelling the presence of cashew nuts in food products is mandatory and consequently, sensitive and specific analytical methods must be developed. In this study, Ana o allergen coding sequences have been sequenced in several cash...

Cashew and pistachio allergies are considered a serious health problem. Previous studies have shown that thermal processing, pressurization and enzymatic hydrolysis may reduce the allergenic properties of food by changing the protein structure. This study assesses the allergenic properties of cashew and pistachio after thermal treatment (boiling an...

A novel electrochemical disposable nucleic acid biosensor for simple, rapid and specific detection of adulterations with horsemeat is reported in this work. The biosensing platform involves immobilization of a 40-mer RNA probe specific for a characteristic fragment of the mitochondrial DNA D-loop region of horse onto the surface of magnetic microca...

A disposable amperometric sensor using magnetic microcarriers has been designed and implemented to be used in combination with the so called Express PCR to detect the presence of hazelnut traces in foodstuffs through the detection of Cor a 9 allergen coding sequence. The developed procedure involves the use of streptavidin-modified magnetic microbe...

Abies pinsapo Boiss. is a relict conifer species that can be found in five cores in the world, three of them in southern Spain and the other two in northern Morocco. The Spanish Fir populations are now threatened by changes in temperatures and precipitations that have been happening in the Mediterranean basin in recent years.To protect this species...

The aim of this study was to investigate changes in walnut allergenicity after processing treatments by in vitro techniques and physiologically relevant assays. The allergenicity of walnuts subjected to high hydrostatic pressure and thermal/pressure treatments was evaluated by IgE-immunoblot and antibodies against walnut major allergen Jug r 4. The...

The aim of this work was to develop and analytically validate a quantitative RT-PCR method, using novel primer sets designed on Pru du 1, Pru du 3, Pru du 4 and Pru du 6 allergen-coding sequences, and contrast the sensitivity and specificity of these probes. The temperature and/or pressure processing influence on the ability to detect these almond...

Food allergies to hazelnut represent an important health problem in industrialized countries because of their high prevalence and severity. Food allergenicity can be changed by several processing procedures since food proteins may undergo modifications which could alter immunoreactivity. High-hydrostatic pressure (HHP) is an emerging processing tec...

The eukaryotic recombinases RAD51 and DMC1 are essential for DNA strand-exchange between homologous chromosomes during meiosis. RAD51 is also expressed during mitosis, and mediates homologous recombination (HR) between sister chromatids. It has been suggested that DMC1 might be involved in the switch from intersister chromatid recombination in soma...

In vitro regenerated plants of rye, Secale cereale L., Ail,s and Merced cultivars, were studied to verify if genetic and/or epigenetic changes were promoted by in vitro conditions. Inter-simple sequence repeat (ISSR) fingerprints on HpaII/MspI-digested and uncut DNA were generated. DNA digested with methylation-sensitive isoschizomers revealed epig...

Regenerated plants obtained from immature embryo-derived calluses of four cultivars of rye, as well as their progeny, were cytologically analyzed. Chromosome number modified plants were found among the regenerants. The progeny of apparently normal diploid regenerated plants was tested and in some cases chromosomally abnormal plants appeared. Equall...

Albino plants have been observed among regenerated plants from immature embryo calluses in four cultivars of rye. The frequency of albino plants was very similar between the plants regenerated from embryogenic (8.78%) or organogenic (12.06%) cultures. However, these frequencies varied widely between cultivars (from 0 to 23.46%). On the other hand,...

Plant species have developed in the course of evolution through a series of mechanisms which optimize their relationships with the environments in which they live. As plants are sessile organisms, they cannot avoid extreme stress situations which can be deleterious. Although the word stress is well known, it has proven to be a very elusive concept,...

Somaclonal variation has been intensively studied by using various molecular markers, however, very little information has been published concerning the nature of the changes and the mutational events involved in the appearance of the marker pattern modifications. Albino plants appeared with a high frequency among the regenerated plants of rye. Whe...

The study of two variable amplicons of rye indicates that RYS1, a mobile element, is activated during tissue culture. We propose that RYS1 could be a foldback (FB) transposon. The FB transposons have been rarely reported in plants; RYS1 is the first described in rye and also the first active plant FB transposon reported. Preferential integration po...

In vitro regeneration of four Begonia genotypes, B. semperflorens, B. rex, B.elatior, and hybrid of Begonia with unknown parents Tiger was carried out starting from leaf and petiole segments as explants. Five Murashige and Skoog''s derived media were tested, three of them supplemented with -naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), and...

Two long primers of 19 (F17) and 20 (F13) nucleotides, respectively,were used in polymerase chain reactions to amplify DNA from differentcultivated barley accessions. These primers can distinguish closely relatedvarieties and, with a unique primer, all the barley accessions analysedshowed a characteristic fingerprint. Sixty per cent and 76% of thef...

We studied the genetic variation generated during in vitro culture of rye (Secale cereale). We analysed the progenies of 4 generations of the plants regenerated from immature embryo cultures and found that rye is a species with a high rate of somaclonal variation. When RAPDs were performed, with DNA from in vitro regenerated plants, variations in t...

On Nov 30, 2004 this sequence version replaced gi:28789511.

We have examined the expression of four genes during somatic embryogenesis in two cultivars of sugarcane, Saccharum officinarum, one drought resistant (JA-605) and the other one sensitive (C-8751), as well as in embryogenic and nonembryogenic tissues treated with abscisic acid (ABA) or after drought stress. Three of the cDNAs probed, from plasmids...

RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences. From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one...

Three random translational -glucuronidase (gus) gene fusions were previously obtained in Arabidopsis thaliana, using Agrobacterium-mediated transfer of a gus coding sequence without promoter and ATG initiation site. These were analysed by IPCR amplification of the sequence upstream of gus and nucleotide sequence analysis. In one instance, the gus s...

Several methods for the extraction of plant DNA have been reported. The protocol described here is a modification of the method developed by Dellaporta et al. (1). This method is generally applicable and has been successfully used on a broad range of tissues, fresh or dried, including calli from many species. The DNA produced is of a moderately hig...

Quantification of DNA is a very important step in many procedures where it is necessary to know the amount of DNA that is present when carrying out restriction digests or performing different techniques such as PCR and RAPDs. There are several methods for quantifying DNA, the most widespread being: (i) the comparison of an aliquot of the extracted...

A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and c...

In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. D...

We studied the genetic variation generated during in vitro culture of rye Secale cereale L.. We analysed the progenies of four generations of the plants regenerated from immature embryo cultures and we found that rye is a species with a high rate of somaclonal variation. When RAPDs were performed, with DNA from in vitro regenerated plants, variatio...

The genus Secale includes 14 species of perennials and annuals, but only one species, Secale cereale L., is extensively cultivated and has an important role in human and animal diets.

We have obtained one plant regenerated from rye tissue culture which showed a high percentage of polyembryonic seeds in its progeny. The mutation inducing the development of extra embryos is also influencing erroneous cell division, mitosis and meiosis. The genetic analysis indicated that the aptitude for polyembryonic seed formation is a heritable...

We studied the genetic variation generated during in vitro culture of rye Secale cereale L. We analyzed the progenies of four generations of the plants regenerated from immature embryo cultures. A high frequency of mutant plants was observed, 50.75%, this frequency was genotype dependent. Other characteristics typical of somaclonal variation were a...

The progeny of polyembryonic Secale cereale L., was used to study the in vitro response of the immature embryos. The formation of embryogenic calli was very high, and this response and its distribution was statistically different to that shown by the normal regenerated plants and the original population. This behaviour seems to be related to a gene...

Immature inflorescences of four cultivars of rye were cultured on Murashige and Skoog medium (MS) with different concentration of 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic calluses were obtained in most cases. However the frequency varied in regard to inflorescence length, 2,4-D concentration and donor cultivar. Evidence is provided that...

Leaf explants of rye (Secale cereale L.), obtained from 3–4-week-old plants growing in aseptic conditions were cultured on the Murashige and Skoog medium (MS) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D). Embryogenic calluses from which plantlets could be obtained were formed in most of the cases.