Rosario Gil

Rosario Gil
University of Valencia | UV · Department of Genetics

PhD in Pharmacy

About

81
Publications
11,015
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4,037
Citations
Citations since 2017
19 Research Items
1244 Citations
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2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Introduction
My research interests focus on functional genomics of endosymbiotic bacteria from insects, minimal genomes, and the evolution of metabolism in bacteria and synthetic biology.
Additional affiliations
May 2018 - present
University of Valencia
Position
  • Coordinator of the Academic Comittee of the PhD Programme in Biodiversity and Evolutionary Biology
March 2017 - July 2020
University of Valencia/CSIC
Position
  • PI at the Scientific Program for Evolutionary Systems Biology of Symbionts
May 2008 - present
Universitat de València
Position
  • Professor
Description
  • Genetics (Degree in Biology, 2nd year); Molecular Genetics (Degree in Biotechnology, 3rd year); Omic technologies (Master in Bioinformatics/Master in Research in Molecular Biology, Cellular Biology and Genetics)

Publications

Publications (81)
Chapter
Aedes albopictus was first reported in Valencia (Eastern Spain) in 2015. Once its establishment was confirmed, several mosquito management actions were applied in order to minimize its impact, including routinely larvicide treatments in public city catch basins, attendance of citizen complaints linked to species activity, private areas' inspections...
Article
Full-text available
The current theoretical proposals of minimal genomes have not attempted to outline the essential machinery for proper translation in cells. Here, we present a proposal of a minimal translation machinery based on (1) a comparative analysis of bacterial genomes of insects' endosymbionts using a machine learning classification algorithm, (2) the empir...
Article
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Mutualistic stable symbioses are widespread in all groups of eukaryotes, especially in insects, where symbionts have played an essential role in their evolution. Many insects live in obligate relationship with different ecto- and endosymbiotic bacteria, which are needed to maintain their hosts’ fitness in their natural environment, to the point of...
Article
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Blattella germanica presents a very complex symbiotic system, involving the following two kinds of symbionts: the endosymbiont Blattabacterium and the gut microbiota. Although the role of the endosymbiont has been fully elucidated, the function of the gut microbiota remains unclear. The study of the gut microbiota will benefit from the availability...
Article
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Background The study of gene essentiality is fundamental to understand the basic principles of life, as well as for applications in many fields. In recent decades, dozens of sets of essential genes have been determined using different experimental and bioinformatics approaches, and this information has been useful for genome reduction of model orga...
Article
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Cockroaches are intriguing animals with two coexisting symbiotic systems, an endosymbiont in the fat body, involved in nitrogen metabolism, and a gut microbiome whose diversity, complexity, role, and developmental dynamics have not been fully elucidated. In this work, we present a metagenomic approach to study Blattella germanica populations not tr...
Article
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Defining the essential gene components for a system to be considered alive is a crucial step toward the synthesis of artificial life. Fifteen years ago, Gil and coworkers proposed the core of a putative minimal bacterial genome, which would provide the capability to achieve metabolic homeostasis, reproduce, and evolve to a bacterium in an ideally c...
Article
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Settled on the foundations laid by zoologists and embryologists more than a century ago, the study of symbiosis between prokaryotes and eukaryotes is an expanding field. In this review, we present several models of insect–bacteria symbioses that allow for the detangling of most known features of this distinctive way of living, using a combination o...
Article
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Aphids (Hemiptera: Aphididae) can harbor two types of bacterial symbionts. In addition to the obligate endosymbiont Buchnera aphidicola Munson, Baumann and Kinsey 1991 (Enterobacteriales: Enterobacteriaceae), several facultative symbiotic bacteria, called secondary (S) symbionts, have been identified among many important pest aphid species. To dete...
Chapter
Symbioses involving prokaryotes living in close relationship with eukaryotic cells have been widely studied from a genomic perspective, especially in the case of insects. In the process toward host accommodation, symbionts undergo major genetic and phenotypic changes that can be detected in comparison with free-living relatives. But, as expected, s...
Article
Full-text available
Many insect species maintain mutualistic relationships with endosymbiotic bacteria. In contrast to their free-living relatives, horizontal gene transfer (HGT) has traditionally been considered rare in long-term endosymbionts. Nevertheless, meta-omics exploration of certain symbiotic models has unveiled an increasing number of bacteria-bacteria and...
Article
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Many insects rely on bacterial endosymbionts to obtain nutrients that are scarce in their highly specialized diets. The most surprising example corresponds to the endosymbiotic system found in mealybugs from subfamily Pseudococcinae in which two bacteria, the betaproteobacterium /`Candidatus Tremblaya princeps/' and a gammaproteobacterium, maintain...
Article
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Buchnera aphidicola is the primary endosymbiont of aphids with which it maintains an obligate mutualistic symbiotic relationship. Insects also maintain facultative symbiotic relationships with secondary symbionts, and Serratia symbiotica is the most common in aphids. The presence of both symbionts in aphids of the subfamily Lachninae has been widel...
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cis-Encoded antisense RNAs (asRNAs) are widespread along bacterial transcriptomes. However, the role of most of these RNAs remains unknown, and there is an ongoing discussion as to what extent these transcripts are the result of transcriptional noise. We show, by comparative transcriptomics of 20 bacterial species and one chloroplast, that the numb...
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The notion of minimal life has sparked the interest of scientists in different fields, ranging from the origin-of-life research to biotechnology-oriented synthetic biology. Whether the interest is focused on the emergence of protocells out of prebiotic systems or the design of a cell chassis ready to incorporate new devices and functions, proposing...
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The evolution of eukaryotic organisms is often strongly influenced by microbial symbionts that confer novel traits to their hosts. Here we describe the intracellular Enterobacteriaceae symbiont of the invasive ant Cardiocondyla obscurior, 'Candidatus Westeberhardia cardiocondylae'. Upon metamorphosis, Westeberhardia is found in gut-associated bacte...
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Many insect species establish mutualistic symbiosis with intracellular bacteria that complement their unbalanced diets. The betaproteobacterium " Candidatus Tremblaya " maintains an ancient symbiosis with mealybugs (Hemiptera: Pseudococcidae), which are classified in subfamilies Phenacoccinae and Pseudococcinae. Most Phenacoccinae mealybugs have "...
Chapter
All known living beings are made of cells, each one of which stores in its genome all of the information required for its correct functioning. The advent of high-throughput sequencing technologies and improvements in bioinformatics tools have allowed the complete sequencing, functional analysis and comparison of thousands of genomes from different...
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Intracellular bacterial supply of essential amino acids is common among sap-feeding insects, thus complementing the scarcity of nitrogenous compounds in plant phloem. This is also the role of the two mealybug endosymbiotic systems whose genomes have been sequenced. In the nested endosymbiotic system from Planococcus citri (Pseudococcinae), "Candida...
Article
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Symbiotic associations between animals and microbes are ubiquitous in nature, with an estimated 15% of all insect species harboring intracellular bacterial symbionts. Most bacterial symbionts share many genomic features including small genomes, nucleotide composition bias, high coding density and a paucity of mobile DNA, consistent with long-term h...
Article
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Many insects maintain intracellular mutualistic symbiosis with a wide range of bacteria which are considered essential for their survival (primary or P-endosymbiont) and typically suffer drastic genome degradation. Progressive loss of P-endosymbiont metabolic capabilities could lead to the recruitment of co-existent facultative endosymbiont (second...
Data
Many insects maintain intracellular mutualistic symbiosis with a wide range of bacteria which are considered essential for their survival (primary or P-endosymbiont) and typically suffer drastic genome degradation. Progressive loss of P-endosymbiont metabolic capabilities could lead to the recruitment of co-existent facultative endosymbiont (second...
Article
Full-text available
Background In all branches of life there are plenty of symbiotic associations. Insects are particularly well suited to establishing intracellular symbiosis with bacteria, providing them with metabolic capabilities they lack. Essential primary endosymbionts can coexist with facultative secondary symbionts which can, eventually, establish metabolic c...
Conference Paper
Full-text available
Introducción La simbiosis mutualista entre bacterias e insectos está muy extendida en la naturaleza y parece ser clave del éxito evolutivo de este grupo de organismos. La γ-proteobacteria Buchnera aphidicola es el endosimbionte primario de casi todas las especies de pulgones [1, 2], con quienes mantiene una relación mutualista obligada de carácter...
Article
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Although bacterial genomes have been traditionally viewed as being very compact, with relatively low amounts of repetitive and non-coding DNA, this view has dramatically changed in recent years. The increase of available complete bacterial genomes has revealed that many species present abundant repetitive DNA (i.e., insertion sequences, prophages o...
Article
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The sequence of the genome of “Candidatus Tremblaya princeps” strain PCVAL, the primary endosymbiont of the citrus mealybug Planococcus citri, has been determined. “Ca. Tremblaya princeps” presents an unusual nested endosymbiosis and harbors a gammaproteobacterial symbiont within its cytoplasm in all analyzed mealybugs. The genome sequence reveals...
Article
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Since the establishment of the symbiosis between the ancestor of modern aphids and their primary endosymbiont, Buchnera aphidicola, insects and bacteria have coevolved. Due to this parallel evolution, the analysis of bacterial genomic features constitutes a useful tool to understand their evolutionary history. Here we report, based on data from B....
Chapter
Full-text available
Symbioses involving prokaryotes living in close relationship with eukaryotic cells have been widely studied from a genomic perspective, especially in the case of insects. In the process toward host accommodation, symbionts experience major genetic and phenotypic changes that can be detected in comparison with free-living relatives. But, as expected...
Article
Full-text available
Engineering a cell with the minimum number of genes, functions or components required for living in a defined environment has become one of the promises of system biology. Theoretical as well as experimental approaches have been undertaken in order to identify essential genes and functions for different model organisms. These experiments have shown...
Article
Recent technical and conceptual advances in the biological sciences opened the possibility of the construction of newly designed cells. In this paper we review the state of the art of cell engineering in the context of genome research, paying particular attention to what we can learn on naturally reduced genomes from either symbiotic or free living...
Article
A model to explain the evolutionary history of animal-bacteria obligatory mutualistic symbiosis is presented. Dispensability of genes and genetic isolation are key factors in the reduction process of these bacterial genomes. Major steps in such genome reductive evolution, leading towards primary endosimbiosis, and the possibility of complementation...
Article
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Tots els organismes vius coneguts estan formats per cèl·lules, i cadascuna d'aquestes cèl· lules emmagatzema la informació necessària per al funcionament en el seu genoma. La possibilitat d'estudiar i comparar els genomes complets de diversos organismes ens permet definir un conjunt d'informació genètica essencial per al manteniment d'una cèl·lula...
Chapter
This chapter explores the potential of peptic nucleic acids (PNAs) as prebiotic and abiotic genetic material. It first presents basic PNA chemistry and illustrates its close similarity with protein. PNA replication and catalysis are also discussed. The chapter concludes by suggesting that PNA is of eminent interest for the synthesis of protocells w...
Article
Genome reduction is a general process that has been studied in numerous symbiotic bacteria associated with insects. We investigated the last stages of genome degradation in Blochmannia floridanus, a mutualistic bacterial endosymbiont of the ant Camponotus floridanus. We determined the tempo (rates of insertion and deletion) and mode (size and numbe...
Article
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Bacteria that establish an obligate intracellular relationship with eukaryotic hosts undergo an evolutionary genomic reductive process. Recent studies have shown an increase in the number of mobile elements in the first stage of the adaptive process towards intracellular life, although these elements are absent in ancient endosymbionts. Here, the g...
Article
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Our understanding of prokaryote-eukaryote symbioses as a source of evolutionary innovation has been rapidly increased by the advent of genomics, which has made possible the biological study of uncultivable endosymbionts. Genomics is allowing the dissection of the evolutionary process that starts with host invasion then progresses from facultative t...
Article
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By integrating data from comparative genomics and large-scale deletion studies, we previously proposed a minimal gene set comprising 206 protein-coding genes. To evaluate the consistency of the metabolism encoded by such a minimal genome, we have carried out a series of computational analyses. Firstly, the topology of the minimal metabolism was com...
Data
Full-text available
Alignment of CRP_058 of C.rudii (dnaG, DNA primase) and its orthologs in E.coli (eco), B. aphidicola from the aphids Acyrtosiphon pisum (buc), Baizongia pistacea (bab), Schizaphis graminum (bas), and Cinara cedri (bcc). The protein in C. ruddii is extensively degraded, and has partial or completely lost two domains that are critical for functionali...
Data
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Schematic representation of amino acid biosynthetic pathways in E. coli (blue arrows), B. aphidicola BAp (green arrows), and C. rudii (red arrows). Only incomplete pathways in C. rudii are shown. Hatched arrows in C. rudii indicate genes that are present in its genome but are greatly degraded. These genes probably have lost their original functiona...
Data
Table summarizing the results of the reannotation of the Carsonella rudii's genome, as described in the main text.
Article
Full-text available
Bacterial symbioses are widespread among insects. The early establishment of such symbiotic associations has probably been one of the key factors for the evolutionary success of insects, since it may have allowed access to novel ecological niches and to new imbalanced food resources, such as plant sap or blood. Several genomes of bacterial endosymb...
Article
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Intracellular bacteria are characterized by genome reduction. The 422,434-base pair genome of Buchnera aphidicola BCc, primary endosymbiont of the aphid Cinara cedri, is approximately 200 kilobases smaller than the previously sequenced B. aphidicola genomes. B. aphidicola BCc has lost most metabolic functions, including the ability to synthesize th...
Article
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Buchnera aphidicola, the primary endosymbiont of aphids, has undergone important genomic and biochemical changes as an adaptation to intracellular life. The most important structural changes include a drastic genome reduction and the amplification of genes encoding key enzymes for the biosynthesis of amino acids by their translocation to plasmids....
Article
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INTRODUCTION Even the simplest unicellular organisms on Earth display an amazing degree of complexity. The question is whether such complexity is a necessary attribute of cellular life or whether, instead, cellular life could also be possible with a much smaller number of molecular components, in the form of what has been called a minimal cell (Lui...
Article
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The study of three genomes of the aphid endosymbiont Buchnera aphidicola has revealed an extraordinary stasis: conservation of gene order and genetic composition of the chromosome, while the chromosome size and number of genes has reduced. The reduction in genome size appears to be ongoing since some lineages we now know to have even smaller chromo...
Article
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Preliminary analysis of two selected genomic regions of Buchnera aphidicola BCc, the primary endosymbiont of the cedar aphid Cinara cedri, has revealed a number of interesting features when compared with the corresponding homologous regions of the three B. aphidicola genomes previously sequenced, that are associated with different aphid species. Bo...
Article
The development of molecular techniques for the study of uncultured bacteria allowed the extensive study of the widespread association between insects and intracellular symbiotic bacteria. Most of the bacterial endosymbionts involved in such associations are gamma-proteobacteria, closely related to Escherichia coli. In recent years, five genomes fr...
Article
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The availability of a large number of complete genome sequences raises the question of how many genes are essential for cellular life. Trying to reconstruct the core of the protein-coding gene set for a hypothetical minimal bacterial cell, we have performed a computational comparative analysis of eight bacterial genomes. Six of the analyzed genomes...
Article
Blochmannia spp. are intracellular endosymbionts of carpener ants (Camponotus spp.). They are localized in specialized cells. the bacteriocytes, intercalated within the midgut tissue of the animals and the oocytes of queens and workers. They are maternally transmitted resulting in a co-speciation of the bacteria and their host animals. The Blochman...
Article
Full-text available
Bacterial symbioses are widespread among insects, probably being one of the key factors of their evolutionary success. We present the complete genome sequence of Blochmannia floridanus, the primary endosymbiont of carpenter ants. Although these ants feed on a complex diet, this symbiosis very likely has a nutritional basis: Blochmannia is able to s...
Article
Full-text available
Bacterial symbioses are widespread among insects, probably being one of the key factors of their evolutionary success. We present the complete genome sequence of Blochmannia floridanus, the primary endosymbiont of carpenter ants. Although these ants feed on a complex diet, this symbiosis very likely has a nutritional basis: Blochmannia is able to s...
Article
Many bacteria, especially proteobacteria, have established a symbiotic association with insects. Insects confer to their partners a stable environment, while the bacteria provide the host with new metabolic capabilities, complementing the insect nutritional requirements and contributing to the exploitation of new ecological niches. The analyses of...
Article
We have characterized industrial yeast strains used in the brewing, baking, and winemaking industries for the presence or absence of cytoplasmic single-stranded 20S and 23S RNAs. Furthermore, the variation of intracellular concentrations of these replicons in brewing and laboratory strains under nutritional stress conditions was determined. Our res...
Article
Full-text available
Buchnera is a mutualistic intracellular symbiont of aphids. Their association began about 200 million years ago, with host and symbiont lineages evolving in parallel since that time. During this coevolutionary process, Buchnera has experienced a dramatic decrease of genome size, retaining only essential genes for its specialized lifestyle. Previous...
Article
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Saccharomyces cerevisiae strains frequently exhibit rather specific phenotypic features needed for adaptation to a special environment. Wine yeast strains are able to ferment musts, for example, while other industrial or laboratory strains fail to do so. The genetic differences that characterize wine yeast strains are poorly understood, however. As...
Article
The 2818 amino acids of neurofibromin, the product of the human NF1 gene, include a 230 amino acid Ras-GAP related domain (GRD). Functions which may be associated with the rest of the protein remain unknown. However, many NF1 mutations in neurofibromatosis 1 patients are found downstream of the GRD, suggesting that the C-terminal region of the prot...
Article
Dysregulation of Wnt–β-catenin signaling disrupts axis formation in vertebrate embryos and underlies multiple human malignancies. The adenomatous polyposis coli (APC) protein, axin, and glycogen synthase kinase 3β form a Wnt-regulated signaling complex that mediates the phosphorylation-dependent degradation of β-catenin. A protein phosphatase 2A (P...