Rosanne Healy

University of Florida | UF · Department of Plant Pathology

Ectomycorrhizal fungi in the genus Tuber form hypogeous fruiting bodies called truffles. Many Tuber species are highly prized due to their edible and aromatic ascomata. Historically, there has been attention on cultivating and selling European truffle species, but there is growing interest in cultivating, wild-harvesting, and selling species of tru...

Cistaceae are shrubs, subshrubs and herbs that often occur in stressful, fire-prone or disturbed environments and form ectomycorrhizal (ECM) associations with symbiotic fungi. Although some Cistaceae are long-lived shrubs that grow to significant size, others are herbaceous annuals or short-lived plants. Thus, Cistaceae are atypical ECM hosts that...

Presentation and illustration of a new species of the genus Paragalactinia, P. pseudomichelii, collected in Canada and the USA, based on morphological and molecular characters. An updated key to the genus Paragalactinia is also provided

Pezizales are a diverse and economically important order of fungi. They are common in the environment, having epigeous form, such as morels and hypogeous, forms called truffles. The mature ascospores of most epigeous Pezizales are forcibly discharged through an opening at the ascus apex created with the lifting of the operculum, a lid-like structur...

Amylascus is a genus of ectomycorrhizal truffles within Pezizaceae that is known from Australia and contains only two described species, A. herbertianus and A. tasmanicus . Species of Amylascus are closely related to truffles ( Pachyphlodes , Luteoamylascus ) and cup fungi ( Plicariella ) from the Northern Hemisphere. Here we reevaluate the species...

In this 9th contribution to the Fungal Systematics and Evolution series published by Sydowia, the authors formally describe 12 species: Bipolaris chusqueae from Chile (Pleosporales); Cortinarius anomalosimilis and C. brunneoviscidus from Canada and the USA, Inocybe nigroumbonata from Pakistan, Mycena amoena from the Netherlands, Tricholoma imbricat...

Species in the genus Tuber are ascomycetous fungi that produce hypogeous fruiting bodies commonly called truffles. These fungi are ecologically relevant owing to the ectomycorrhizal symbiosis they establish with plants. One of the most speciose lineages within Tuber is the Rufum clade, which is widely distributed throughout Asia, Europe, and North...

Species of the genus Phaeohelotium (Leotiomycetes: Helotiaceae) are cup fungi that grow on decaying wood, leaves, litter, and directly on soil. Northern Hemisphere species are primarily found on litter and wood, whereas in the Southern Hemisphere the genus includes a mix of saprotrophs as well as taxa that grow on soil in association with ectomycor...

The occurrence and distribution of morels in Nothofagaceae forests of South America are addressed based on our field studies in Southern Chile and Argentina. Both ascomata and mitosporic colonies were collected. In addition, ascomata were procured from commercial harvesters. A four-gene (ITS, RPB1, RPB2, TEF1-α) and ITS phylogeny assigned these Mor...

The occurrence and distribution of morels in Nothofagaceae forests of South America is addressed based on our field studies in Southern Chile and Argentina. Both ascomata and mitosporic colonies were collected and ascomata were procured from commercial harvesters. A four-gene (ITS, RPB1 , RPB2 , TEF1-α ) and ITS phylogeny assigned these Morchella a...

In this study we review recent collections and historical records of epigeous members of the Pezizales formerly placed in the large, heterogenous genus Peziza from temperate southern South America. Recent analyses using molecular phylogenetic methods allow placement of these species in several previously described genera in recognition of the heter...

The ectomycorrhizal truffle genus Tuber is widespread and diverse. Recent sampling of ascomata, ectomycorrhizal root tips, and environmental sequences has resulted in the identification of many Tuber species that cannot be assigned to described species and require formal description. Using morphological and molecular phylogenetic analysis, we descr...

The boletoid genera Butyriboletus and Exsudoporus have recently been suggested by some researchers to constitute a single genus, and Exsudoporus was merged into Butyriboletus as a later synonym. However, no convincing arguments have yet provided significant evidence for this congeneric placement. In this study, we analyze material from Exsudoporus...

Fungal endophytes live asymptomatically within plants and are widespread inhabitants of leaves and other organs (Wilson, 1995). Similarly, endolichenic fungi live asymptomatically within lichens, occurring in healthy lichen thalli worldwide (Arnold et al., 2009). Endophytes and endolichenic fungi are ecologically similar, living in symbiosis with e...

Among many convergently evolved sequestrate fungal genera in Boletaceae ( Boletales , Basidiomycota ), the genus Octaviania is the most diverse. We recently collected many specimens of Octaviania subg. Octaviania, including several undescribed taxa, from Japan and the Americas. Here we describe two new species in subgenus Octaviania , O. tenuipes a...

Orbilia xanthostigma, with golden yellow to yellow-orange apothecia and O. leucostigma, with white to very pale rose-lilaceous apothecia, were described by E.M. Fries over 200 years ago. Each of the two taxa, which are not easy to interpret because type material is lacking, was proposed in the past as lectotype of the genus Orbilia. In the here pre...

Pachyphlodes is a lineage of ectomycorrhizal, hypogeous, sequestrate ascomycete fungi native to temperate and subtropical forests in the Northern Hemisphere. Pachyphlodes species form ectomycorrhizae mainly with Fagales hosts. Here we describe two new species of Pachyphlodes , P. brunnea , and P. coalescens , based on morphological and phylogenetic...

Fungi in the Nidulariaceae, otherwise known as ‘bird’s nest fungi’, are among the least studied groups of Agaricomycetes (Basidiomycota). Bird’s nest fungi are globally distributed and typically grow on woody debris or animal dung as saprotrophs. This group of fungi is morphologically diverse with ca. 200 described species. Phylogenetic relationshi...

Among many convergently evolved sequestrate fungal genera in Boletaceae ( Boletales , Basidiomycota ), the genus Octaviania is the most diverse. We recently collected many specimens of Octaviania subgenus Octaviania , including several undescribed taxa, from Japan and the Americas. Here we describe two new species in subgenus Octaviania , O. tenuip...

Molecular phylogenetic analyses have addressed the systematic position of several major Northern Hemisphere lineages of Pezizales but the taxa of the Southern Hemisphere remain understudied. This study focuses on the molecular systematics and taxonomy of Southern Hemisphere species currently treated in the genera Underwoodia and Gymnohydnotrya . Sp...

A second series of species of the genus Tricharina is reviewed based on morphological and phy-logenetic data. The examination of Tricharina cretea in the sense of Thind & Waraitch resulted in its description as a new species, published herein as T. indica. T. hiemalis, T. japonica, and T. striispora are described and illustrated with new collection...

The genus Hygrophorus is poorly studied from Asia. From Pakistan, only one species has been reported so far. Two new species in the genus have been collected from Himalayan oak forests of Pakistan. Hygrophorus alboflavescens (section Pudorini, subgenus Colorati) is characterised by its pure white, centrally depressed pileus, occurrence of white sti...

Molecular investigations into two truffle-like fungi within the Pyronemataceae (Pezizales, Ascomycota) revealed hidden diversity that required further clarification of generic and species boundaries of the hypogeous, ptychothecial members of this family. Here we describe Terracavicola echinospora gen. et. sp. nov. from mexico and we transfer Pachyp...

Ruhlandiella is a genus of exothecial, ectomycorrhizal fungi in the order Pezizales. Ascomata of exothecial fungi typically lack a peridium and are covered with a hymenial layer instead. Ruhlandiella species have nonoperculate asci and highly ornamented ascospores. The genus was first described by Hennings in 1903 to include the single species, R....

Hortiboletus kohistanensis is described as a new species from the moist temperate and subalpine oak forests of Pakistan after detailed morphological and phylogenetic analyses as well as comparison with related taxa. This oak-associated species is morphologically distinguished from allied taxa by an areolate pileus surface and whitish stipe base. It...

Novel species of fungi described in this study include those from various countries as follows: Angola, Gnomoniopsis angolensis and Pseudopithomyces angolensis on unknown host plants. Australia, Dothiora corym­ biae on Corymbia citriodora, Neoeucasphaeria eucalypti (incl. Neoeucasphaeria gen. nov.) on Eucalyptus sp., Fumagopsis stellae on Eucalyptu...

Truffles are sequestrate hypogeous fungi, and most form ectomycorrhizal (ECM) associations with trees. Truffles belonging to the genus Tuber (Pezizales, Ascomycota), “true truffles,” associate with diverse plant hosts, including economically important species such as pecan (Carya illinoinensis). Morphological and phylogenetic studies delimited seve...

A multigene phylogenetic analysis of Genabea, Myrmecocystis and related genera in Pyronemataceae was conducted to establish genetically supported generic limits. The nuc rDNA 28S gene, translation elongation factor 1-α (TEF1) and RNA polymerase II second largest subunit gene (RPB2) significantly supported the monophyly of several distinct genera of...

Tuber aztecorum sp. nov., a truffle species from Mexico belonging to the Maculatum clade (Tuberaceae, Pezizales). Gonzalo Guevara-Guerrero, Gregory Bonito, Matthew E. Smith, Rosanne Healy, Arthur C. Grupe II, Efrén Cázares, Michael A. Castellano, James M. Trappe. A new species of truffle, T.aztecorum , is described from central Mexico. Tuberazt...

Pachyphlodes (Pezizaceae) is a genus of truffle-like fungi that is distributed across the Northern Hemisphere. These fungi form ectomycorrhizae primarily with trees in the Fagaceae family, and occasionally with other host plants. The genus Plicariella (= Scabropezia) is phylogenetically inferred as an ally of, or within, the Pachyphlodes lineage. D...

Tricharina is one of the most difficult genera of Pezizales because it is hard to distinguish morphologically among species. To provide a more robust taxonomy, new investigations on the genus were conducted, both morphologically and phylogenetically. This study focuses on the four key species of the genus: T. ascophanoides, T. gilva, T. ochroleuca...

Among fungi isolated from healthy root mycobiomes of Populus, we discovered a new endorrhizal fungal species belonging to the rust lineage Pucciniomycotina, described here as Atractiella rhizophila. We characterized this species by transmission electron microscopy (TEM), phylogenetic analysis, and plant bioassay experiments. Phylogenetic sequence a...

Truffle species within the /tarzetta-geopyxis lineage share smooth, globose, hyaline spores, but differ in the amount of convolution of hymenia in ascomata. The relationships among truffle species in this lineage have historically been confused. Phylogenetic analyses of the ITS and 28S nuclear ribosomal DNA from recently collected members of the /t...

A species of Pseudotricharina, similar in sequence and morphology to the type species P. intermedia, is described from a soil bank in a Nothofagus forest of the Andes Mountains of Argentina. This is only the second species of Pseudotricharina to be described and the first known from the Southern Hemisphere.

Following the change that eliminated dual naming of sexual and asexual morphs of fungi, generic names of Pezizomycetes have been evaluated to determine which of the competing names should be recommended for use. Evaluation is based on congruence of type species to determine if the names are congeneric and which name is most commonly cited as well a...

During a study comparing the ectomycorrhizal root communities in a native forest with those at the Arnold Arboretum in Massachusetts (USA), the European species Tuber borchii was detected on the roots of a native red oak in the arboretum over two successive years. Since T. borchii is an economically important edible truffle native to Europe, we con...

The Tuber rufum clade is one of the most speciose of clades in the Tuberaceae and is found in the Northern Hemisphere primarily in Asia, Europe, and North America. The species are ectomycorrhizal with angiosperms in neutral to high pH soils. The Rufum clade consists of an estimated 43 species, of which only 18 have been described. Molecular data su...

This paper documents the colonization of the roots of Celastrus orbiculatus by endomycorrhizal fungi in field-collected specimens in New England. The presence of endomycorrhizal fungi in the roots of C. orbiculatus in its introduced range may assist this species in its successful colonization of new habitats.

The new taxon Luteoamylascus aculeatus described in this article is proposed to accommodate two collections of a hypogeous ascomycete from central Spain, characterized by a tomentose yellowish peridium, labyrinth-like gleba filled with whitish hyphae, and intensely reacting amyloid asci. ITS, 28S, and RPB2 data suggest that this new taxon is an ind...

A Dacryopinax species that was cultured in Costa Rica and fruited in the laboratory provided DNA for the first sequenced genome for the Dacrymycetes. Here we characterize the isolate morphologically and cytologically and name it D. primogenitus Molecular sequences from the nuclear large subunit gene and internal transcribed spacer indicated that it...

• The earliest eukaryotes were likely flagellates with a centriole that nucleates the centrosome, the microtubule-organizing center (MTOC) for nuclear division. The MTOC in higher fungi, which lack flagella, is the spindle pole body (SPB). Can we detect stages in centrosome evolution leading to the diversity of SPB forms observed in terrestrial fun...

Detailed description and illustrations of Trichophaea flavobrunnea (Richon) Priou, Perić, Van Vooren & Hairaud comb. nov., rare species, characterized by relatively large apothecia, by the outer surface and the hairs sometimes covered with a gel, by smooth ascospores and its habitat on needles litter of Juniperus or Cupressus. This taxon is highlig...

Neolecta represents the earliest derived extant ascomycete lineage (Taphrinomycotina) to produce ascomata. For this reason the genus has been of interest with regard to ascoma evolution in ascomycetes. However, the evidence is equivocal regarding whether the Neolecta ascoma is homologous or analogous to ascomata produced in the later derived ascomy...

Neolecta represents the earliest derived extant ascomycete lineage (Taphrinomycotina) to produce ascomata. For this reason the genus has been of interest with regard to ascoma evolution in ascomycetes. However, the evidence is equivocal regarding whether the Neolecta ascoma is homologous or analogous to ascomata produced in the later derived ascomy...

Truffles have evolved from epigeous (aboveground) ancestors in nearly every major lineage of fleshy fungi. Because accelerated rates of morphological evolution accompany the transition to the truffle form, closely related epigeous ancestors remain unknown for most truffle lineages. This is the case for the quintessential truffle genus Tuber, which...

Economically important Tuber species determined by 2009 market prices (USD) in the USA. (XLS)

Collection information and GenBank accession information for taxa sampled. (XLS)

Primers used in this study. (XLS)

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Fungal mitospores may function as dispersal units and/ or spermatia and thus play a role in distribution and/or mating of species that produce them. Mitospore production in ectomycorrhizal (EcM) Pezizales is rarely reported, but here we document mitospore production by a high diversity of EcM Pezizales on three continents, in both hemispheres. We s...

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##