About
12
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Introduction
Graduated in 2016 at the University of Naples in Medical Biotechnologies with an experimental thesis in immunology, performed at the Institute of Protein Biochemistry,CNR. In 2021 she received her PhD in Biology and she is now a PostDoc at the Institute of Biosciences and Bioresources (IBBR - UOS Naples) of the CNR. Her research interests include the characterization of novel O6-DNA-alkyltranferases as thermostable protein-tags and their applications in biotechnological fields.
Current institution
Education
January 2018 - May 2021
March 2014 - December 2016
October 2010 - March 2014
Publications
Publications (12)
Self-labelling protein tags (SLPs) are resourceful tools that revolutionized sensor imaging, having the versatile ability of being genetically fused with any protein of interest and undergoing activation with alternative probes specifically designed for each variant (namely, SNAP-tag, CLIP-tag and Halo-tag). Commercially available SLPs are highly u...
The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage activity of Cas12a enzymes using hairpin DNA sequences as FRET-based reporters. We discover faster rate of trans-cleavage activity of Ca...
In the past decade, a powerful biotechnological tool for specific in vivo and in vitro labeling of proteins of interest, the SNAP-tag technology, has been proposed as a valid alternative to the classic protein-tags. This self-labeling protein-tag is an engineered variant of the human O6-alkylguanine-DNA-alkyltransferases (hMGMT) enzyme, which speci...
We present a new class of DNA‐based nanoswitches, named folding‐upon‐repair DNA nanoswitches, that upon enzymatic repair could undergo a conformational change mechanism leading to a change in fluorescent signal. The nanoswitches are suitable substrates for different methyltransferases and allow the screening of novel potential methyltransferase inh...
We present a new class of DNA‐based nanoswitches that, upon enzymatic repair, could undergo a conformational change mechanism leading to a change in fluorescent signal. Such folding‐upon‐repair DNA nanoswitches are synthetic DNA sequences containing O⁶‐methyl‐guanine (O⁶‐MeG) nucleobases and labelled with a fluorophore/quencher optical pair. The na...
SNAP-tag® is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates. Although commercially available or ad hoc produced, their synthesis and purification are necessary, increasing time and costs. To address this limitation, here we suggest a revision of this methodology, by performing a che...
The genome of living cells is continuously exposed to endogenous and exogenous attacks, and this is particularly amplified at high temperatures. Alkylating agents cause DNA damage, leading to mutations and cell death; for this reason, they also play a central role in chemotherapy treatments. A class of enzymes known as AGTs (alkylguanine-DNA-alkylt...
The specific labelling of proteins in recent years has made use of self-labelling proteins, such as the SNAP-tag® and the Halotag®. These enzymes, by their nature or suitably engineered, have the ability to specifically react with their respective substrates, but covalently retaining a part of them in the catalytic site upon reaction. This led to t...
Carbonic anhydrases (CAs, EC 4.2.1.1) are a superfamily of ubiquitous metalloenzymes present in all living organisms on the planet. They are classified into seven genetically distinct families and catalyse the hydration reaction of carbon dioxide to bicarbonate and protons, as well as the opposite reaction. CAs were proposed to be used for biotechn...
The use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of proteins on the outer membrane of Gram-negative bacteria. These strategies have the clear advantage of unifying in a one-step the production, the purification and the...
