Rosa A Maldonado

Rosa A Maldonado
University of Texas at El Paso | UTEP · Department of Biological Sciences

Doctor in Biological Sciences

About

55
Publications
4,417
Reads
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851
Citations
Additional affiliations
October 2004 - present
University of Texas at El Paso
Position
  • Professor (Associate)
Education
January 1997 - May 1999
University of Dundee
Field of study
  • Molecular Parasitology
June 1992 - August 1996
Federal University of Rio de Janeiro
Field of study
  • Biology - Molecular Parasitology
March 1982 - June 1989
Central University of Venezuela
Field of study
  • Biology

Publications

Publications (55)
Article
Full-text available
Introduction Chagas disease (CD) affects ~7 million people worldwide. Benznidazole (BZN) and nifurtimox (NFX) are the only approved drugs for CD chemotherapy. Although both drugs are highly effective in acute and paediatric infections, their efficacy in adults with chronic CD (CCD) is lower and variable. Moreover, the high incidence of adverse even...
Article
Chagas disease is a parasitic illness caused by Trypanosoma cruzi. This is an intracellular parasite that can cause cardiomyopathy, megaesophagus and megacolon in the chronic phase of the disease. One of the characteristics is sudden death due to heart failure. In this study, a 15-month-old, intact female bat-eared fox (Otocyon megalotis) kept in a...
Article
Full-text available
The causative agent of Chagas disease, Trypanosoma cruzi , is transmitted by triatomine vectors. The insect is endemic in the Americas, including the United States, where epidemiological studies are limited, particularly in the Southwestern region. Here, we have determined the prevalence of T . cruzi in triatomines, and feral cats and dogs, and wil...
Article
Full-text available
Leishmania major is the causative agent of cutaneous leishmaniasis (CL). No human vaccine is available for CL and current drug regimens present several drawbacks such as emerging resistance, severe toxicity, medium effectiveness, and/or high cost. Thus, the need for better treatment options against CL is a priority. In the present study, we validat...
Article
Full-text available
Chagas disease (ChD), caused by the hemoflagellate parasite Trypanosoma cruzi, affects six to seven million people in Latin America. Lately, it has become an emerging public health concern in nonendemic regions such as North America and Europe. There is no prophylactic or therapeutic vaccine as yet, and current chemotherapy is rather toxic and has...
Chapter
Chagas disease (ChD), caused by the protozoan parasite Trypanosoma cruzi, affects millions of people worldwide. Chemotherapy is restricted to two drugs, which are partially effective and may cause severe side effects, leading to cessation of treatment in a significant number of patients. Currently, there are no biomarkers to assess therapeutic effi...
Article
Full-text available
Leishmania major (L. major) is a protozoan parasite that causes cutaneous leishmaniasis. About 12 million people are currently infected with an annual incidence of 1.3 million cases. The purpose of this study was to synthesize a small library of novel thiophene derivatives, and evaluate its parasitic activity, and potential mechanism of action (MOA...
Article
Full-text available
Background: Protozoan parasites from the genus Leishmania cause broad clinical manifestations known as leishmaniases, which affect millions of people worldwide. Cutaneous leishmaniasis (CL), caused by L. major, is one the most common forms of the disease in the Old World. There is no preventive or therapeutic human vaccine available for L. major C...
Data
Levels of IL-6 and IL-17 in the mouse sera following immunization with NGP5B or NGP5B+CpG. IL-6 and IL-17 were analyzed in the sera of immunized mice three weeks after last immunization (B3), prior to challenge with L. major-luc (day 0, Fig 4A), as described in Materials and Methods. No significant difference was observed between NGP5B or NGP5B+CpG...
Article
In previous studies we reported a novel series of organometallic compounds, RuII complexed with clotrimazole, displaying potent trypanosomatid activity with unnoticeable toxicity toward normal mammalian cells. In view of the promising activity of Ru-clotrimazole complexes against Leishmania major (L. major), the present work sought to investigate t...
Article
Full-text available
Background: Trypanosoma cruzi causes Chagas disease, an endemic and debilitating illness in Latin America. Lately, owing to extensive population movements, this neglected tropical disease has become a global health concern. The two clinically available drugs for the chemotherapy of Chagas disease have rather high toxicity and limited efficacy in t...
Data
T. cruzi myristoylated proteins are not susceptible to base treatment. In-gel western blot of Epi lysate was treated (+ NaOH) or not (- NaOH) with 0.2 M NaOH in methanol to remove any base-labile hydroxy- or thioester-linked myristic acid azide, followed by “click” reaction with biotin alkyne. IRDye 800CW streptavidin was used to detect myristoylat...
Data
DDD compounds do not affect protein translation. Fluorographs of PVDF membranes of [35S]-methionine-labeled lysates from Epi, ICA, and TCT forms treated or not with 10 μM of the compound 1, 5, or 8. (TIF)
Data
Anti-LmNMT and anti-TbNMT recognize TcNMT. Immunofluorescence microscopy of Epi stained with anti-LmNMT and anti-TbNMT (red), co-stained with DAPI (blue) to reveal positions of the nucleus and kinetoplast (blue). Scale bar, 5 μm. (TIF)
Data
DDD compounds inhibit intracellular proliferation of T. cruzi. The multiparametric data obtained on a cell-by-cell basis by HCI was analyzed to determine several parameters associated to infection of host cells by T. cruzi. (A and B) Percentage of cells infected with at least one parasite (percentage of infected cells). (C and D) Percentage of cell...
Data
DDD compound 2 is not trypanocidal against purified intracellular amastigotes. Total number of cells in each well was counted by HCI to evaluate the cytotoxicity of the compound 2 against purified ICA forms. C, controls: Untreated, DMSO, and H2O2. (TIF)
Data
DDD compounds are toxic against mammalian cells only at high concentrations. Total number of cells in each well was counted by HCI to evaluate the cytotoxicity of the compounds 1, 5, and 8 against U2OS cells. C, controls: Untreated, DMSO, and H2O2. (TIF)
Article
Leishmania major (L. major) is a protozoan parasite causal agent of Leishmaniasis. It is estimated that 12 million people are currently infected and around 2 million infections occur each year. Current treatments suffer of high toxicity for the patient, low efficacy toward the parasite, high cost, and are losing effectiveness due to parasite resist...
Article
Chagas disease, caused by the parasite Trypanosoma cruzi, is an emerging infectious disease in the United States. In our study, 24 out of 39 triatomines, from the specie Triatoma rubida, were infected with Trypanosoma cruzi. Additionally, only the genotype TcI was characterized among the parasite specimens. Improved knowledge of local epidemiology...
Article
Full-text available
Long-term exposure to arsenic has been linked to cancer in different organs and tissues, including skin. Here, non-malignant human keratinocytes (HaCaT) were exposed to arsenic and its effects on microRNAs (miRNAs; miR) expression were analyzed via miRCURY LNA array analyses. A total of 30 miRNAs were found differentially expressed in arsenic-treat...
Article
In this study we assessed the antileishmanial activity of 126 α,β-unsaturated ketones. The compounds NC901, NC884, and NC2459 showed high leishmanicidal activity for both the extracellular (EC50 = 456 nM, 1122 nM, and 20 nM, respectively), and intracellular forms of Leishmania major propagated in macrophages (EC50 = 1870 nM, 937 nM, and 625 nM, res...
Article
Chagas disease, caused by Trypanosoma cruzi, is responsible for producing significant morbidity and mortality throughout Latin America. The disease has recently become a public health concern to nonendemic regions like the U.S. and Europe. Currently there are no fully effective drugs or vaccine available to treat the disease. The mucin-associated s...
Article
Full-text available
In our ongoing search for new metal-based chemotherapeutic agents against leishmaniasis and Chagas disease, six new ruthenium-ketoconazole (KTZ) complexes have been synthesized and characterized, including two octahedral coordination complexes-cis,fac-[Ru(II)Cl2(DMSO)3(KTZ)] (1) and cis-[Ru(II)Cl2(bipy)(DMSO)(KTZ)] (2) (where DMSO is dimethyl sulfo...
Article
Full-text available
The production of viable cysts by Giardia is essential for its survival in the environment and for spreading the infection via contaminated food and water. The hallmark of cyst production (also known as encystation) is the biogenesis of encystation-specific vesicles (ESVs) that transport cyst wall proteins to the plasma membrane of the trophozoite...
Conference Paper
Currently, only one drug is available for the treatment of Leishmaniasis. For this reason, there is an urgent need to improve the chemotherapeutic methods against Leishmaniasis. Azoles derivatives such as clotrimazole (CTZ) have been found to exert their therapeutic effect through a mechanism involving the inhibition of the biosynthesis of sterols...
Article
Eight new ruthenium complexes of clotrimazole (CTZ) with high antiparasitic activity have been synthesized, cis,fac-[Ru(II)Cl(2)(DMSO)(3)(CTZ)] (1), cis,cis,trans-[Ru(II)Cl(2)(DMSO)(2)(CTZ)(2)] (2), Na[Ru(III)Cl(4)(DMSO)(CTZ)] (3), Na[trans-Ru(III)Cl(4)(CTZ)(2)] (4), [Ru(II)(η(6)-p-cymene)Cl(2)(CTZ)] (5), [Ru(II)(η(6)-p-cymene)(bipy)(CTZ)][BF(4)](2...
Conference Paper
Leishmania major is the ethological agent of cutaneous leishmaniasis (CL) that affects millions of people worldwide. Currently, there are limited medications to treat parasites especially since new drug resistant strains have been discovered. Therefore, the developments of new drug therapies are necessary. The pyruvate phosphate dikinase (PPDK) wor...
Conference Paper
Trypanosoma cruzi is the etiological agent that causes Chagas’ disease. It currently affects 11-16 million people in Latin America. It is an emerging disease throughout the world because of immigration and lack of screening. There is an urgent need to develop a vaccine to be able to control this parasitic infection. In our study we have used a muci...
Conference Paper
Currently, there is only one commercial drug available against Trypanosoma cruzi. This parasite affects millions in Latin America and is an emergent concern in the U.S. New drug-resistant strains clearly point out the urgent need for new drugs. N-myristoyltransferase catalyzes the attachment of myristic acid many eukaryotic proteins. This ubiquitou...
Article
Trypanosoma cruzi affects millions of people worldwide. This unicellular eukaryotic organism is the causative agent of Chagas’ disease. This blood-borne pathogen is transmitted to humans by the hemophagous triatomine or kissing bug. The high toxicity of existing treatments and the lack of vaccines clearly demonstrate the demand for new drugs to tre...
Article
Full-text available
Cationic steroid antibiotics (CSAs), or ceragenins, are amphiphilic compounds consisting of a cholic acid backbone that is attached to several cationic amines. In this study, we tested the hypothesis that CSAs possess antiparasitic activities with minimal to no effects on mammalian cells, and thus could be used as potential therapeutic agents again...
Conference Paper
The protozoan trypanosome Leishmania major is an obligate intracellular parasite that is the causative agent for cutaneous leishmaniasis. This disease is endemic is many regions such as Asia, Africa, South America, Latin America, and with the constant deployment of U.S. troops to endemic areas it has now become a major concern for the U.S. as well....
Article
Arthropods display different mechanisms to protect themselves against infections, among which antimicrobial peptides (AMPs) play an important role, acting directly against invader pathogens. We have detected several factors with inhibitory activity against Candida albicans and Micrococcus luteus on the surface and in homogenate of eggs of the tick...
Article
Full-text available
Chagas disease, caused by Trypanosoma cruzi, is a widespread infection in Latin America. Currently, only 2 partially effective and highly toxic drugs, i.e., benznidazole and nifurtimox, are available for the treatment of this disease, and several efforts are underway in the search for better chemotherapeutic agents. Here, we have determined the try...
Article
Full-text available
Although encystation (cyst formation) is important for the survival of Giardia lamblia outside its human host, the molecular events that prompt encystation have not been fully elucidated. Here, we demonstrate that sphingolipids (SLs), which are important for the growth and differentiation of many eukaryotes, play key roles in giardial encystation....
Article
Gomesin is an 18-residue cysteine-rich antimicrobial peptide produced by hemocytes of the spider Acanthoscurria gomesiana. In the present study, the antifungal properties of gomesin against Cryptococcus neoformans, the etiologic agent of cryptococcosis, were evaluated. Gomesin bound to the cell surface of cryptococci, which resulted in cell death a...
Article
Full-text available
Trypanosoma cruzi lipids contain a high content of unsaturated fatty acids, primarily oleic acid (C18:1) and linoleic acid (C18:2). Previous data suggest that this parasite is able to convert oleic acid into linoleic acid; humans are not able to do this. Presently, we show that T. cruzi has a gene with high similarity to the delta12 (omega6)-oleate...
Article
Full-text available
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA,...
Article
Full-text available
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA,...
Article
We have cloned and characterised a gene that encodes a putative pyruvate phosphate dikinase (PPDK) from Trypanosoma cruzi, an enzyme that catalyses the reversible conversion of phosphoenolpyruvate to pyruvate. PPDK is absent in mammalian cells, but has been found in a wide variety of other organisms, including plants and bacteria. In T. cruzi, two...
Article
The 24 kDa flagellar calcium binding protein (FCaBP) of the protozoan Trypanosoma cruzi is a calcium-acyl switch protein. FCaBP is modified by the addition of myristate and palmitate at its amino terminal segment and both modifications are required for calcium-modulated flagellar membrane association. FCaBP has four sequence motifs for potential ca...
Article
A full-length cDNA encoding the 24-kDa flagellar Ca(2+)-binding protein (FCaBP) of the Dm28c clone of Trypanosoma cruzi was cloned and characterized. Comparison of the deduced amino acid sequence with those of the FCaBPs of other T. cruzi strains revealed greater than 97% sequence conservation. FCaBP-like genes are found in Trypanosoma conorhini, T...
Article
Full-text available
We report the effects of ketoconazole and the bistriazole ICI 195,739 acting alone or in combination with the allylamine terbinafine (Lamisil) on murine models of Chagas' disease. Mice infected with 10(5) Trypanosoma (Schizotrypanum) cruzi blood trypomastigotes and treated orally with 30 mg of ketoconazole per kg of body weight per day for 7 days,...
Article
Full-text available
We have studied the antiproliferative effects of mevinolin (lovastatin), an inhibitor of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase, on the protozoan parasite Trypanosoma (Schizotrypanum) cruzi and its ability to potentiate the action of specific ergosterol biosynthesis inhibitors, such as ketoconazole and terbinafine, both in vitro and in vi...
Article
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Garamond} span.s1 {font: 12.0px 'Times New Roman'} span.s2 {font: 7.0px Garamond} Cutaneous leishmaniasis is a vector-borne disease caused by Leishmania major affecting millions of people throughout the world. It is an emerging concern in the United States due to military establishments in endemic...

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