Rongxia Liu

University of Vienna | UniWien · Department of Pharmacognosy

Poster presented at EMBO Conference on Cellular Signaling & Molecular Medicine 25‐29 May 2012, Cavtat‐Dubrovnik, Croatia. This poster is published under a CC-BY license: http://dx.doi.org/10.6084/m9.figshare.1101323

Cytotoxicity determination upon prolonged exposure and in the presence of higher concentrations of plumericin. HEK293/NF-κB-luc cells were treated as indicated in Figure 1A and Figure 1B with solvent vehicle (0.1% DMSO), different concentrations of plumericin, or digitonin (200 μg·mL-1) as positive control. LDH enzyme activity was determined 24 h a...

Comparison of the effects of plumericin, parthenolide, and the H. sucuuba extract on NF-κB activity in the presence and absence of TNF-α stimulation. HEK293/NF-κBluc cells were pretreated for 30 min with H. sucuuba extract (60 μg·mL-1), plumericin (5 μμ), parthenolide (5 μμ), or solvent vehicle (DMSO 0.1%), and stimulated with 2 ng·mL-1 TNF-α or tr...

Plumericin and the other investigated compounds do not affect the basal expression of the endothelial adhesion molecules VCAM-1, ICAM-1, and E-selectin in the absence of TNF-α stimulation. HUVECtert cells were treated with 10 μμ plumericin, parthenolide, plumieridin, allamandicin, plumeridoid C or 10 ng·mL-1 TNF-α for 14 h (VCAM-1, ICAM-1) or 5 h (...

Effect of reference inhibitors on the investigated phosphorylation sites of JNK, p38, and ERK1/2. HUVECtert cells were pre-incubated for 30 min with SP600125 (50 μμ), forskolin (20 μμ), U0126 (10 μμ) or solvent vehicle (DMSO, 0.1% or 0.2%, respectively), prior to stimulation with TNF-α (10 ng·mL-1) for 10 min.Western blot analysis was performed for...

Among all isolated compounds only plumericin is a potent inhibitor of TNF-α-induced NF-κB activation. HEK293/NF-κB-luc cells were pretreated for 30 min with the indicated compounds (10 μMeach) or solvent vehicle (DMSO 0.1%), and stimulated with 2 ng·mL-1 TNF-α for 4 h. The data represent mean ± SEM (n = 3; *P < 0.05, **P < 0.01, n.s. not significan...

Plumericin but not the other structurally related compounds isolated from H. sucuuba inhibits TNF-α-induced cell surface expression of the endothelial adhesion molecules VCAM-1, ICAM-1, and E-selectin. HUVECtert cells were pretreated with 10 μμ plumericin, parthenolide, plumieridin, allamandicin, plumeridoid C or solvent vehicle (DMSO 0.1%) as cont...

The transcription factor nuclear factor kappa B (NF-κB) orchestrates many pro-inflammatory signals and its inhibition is considered a promising strategy to combat inflammation. Here we report the characterization of the natural product plumericin as a highly potent inhibitor of the NF-κB pathway with a novel chemical scaffold, which was isolated vi...

Seven new triterpenoids, namely heteroclitalactones G-M (1-7), were isolated from the ethanol extract of the stems of Kadsura heteroclita. Structures of these compounds were characterized by extensive 1D and 2D NMR spectroscopic analyses.

Four major triterpenoids (ganoderic acids C(2), B, K and H) in rat plasma after oral administration of G. lucidum extract were analyzed quantitatively by high-performance liquid chromatography (HPLC). Plasma samples taken from rats were acidified with hydrochloric acid and extracted with dichloromethane-ethyl acetate (90:10). The chromatographic se...

Microbial metabolism of evodiamine by Penicillium janthinellum AS 3.510 was investigated. Two metabolites were isolated from the broth and their structures were identified as 10-hydroxyevodiamine and 11-hydroxyevodiamine, by means of their IR, MS and NMR spectral data. Both of the metabolites were proved to be new compounds and showed strong cytoto...

Phytochemical investigations of the stem of Kadsura heteroclita (Roxb) Craib (Schizandraceae) resulted in the isolation and structure elucidation of six new triterpenoidal compounds named heteroclitalactones A-E (1-5) as well as heteroclic acid (6) and heteroclitalactone F (7), which was isolated for the first time from a natural source, and the si...

Two new C(18) dibenzocyclooctadiene lignans named heteroclitalignans A and B (1 and 3), one new C(19) homolignan named heteroclitalignan C(4) and one new natural product named heteroclitalignan D(2), together with the four known lignans kadsurarin (5), kadsulignan E (6), d-epigalbacin, and meso-dihydroguaiaretic acid were isolated from the stems of...

The stem of Kadsura heteroclita is a traditional Chinese medicine with a variety of biological activities. For efficient monitoring of the quality of the herb, a simple, rapid, and accurate HPLC method has been developed for simultaneous determination of five cyclolanostane triterpenoid compounds (schisanlactone E, nigranoic acid, schisandronic aci...

Flavonoids are the main active constituents of Dalbergia odorifera. The excretion of the major flavonoids in rat urine after oral administration of D. odorifera extract was investigated by HPLC-UV and HPLC-MS methods. Utilizing the HPLC-MS technique, 18 flavonoids, including five isoflavones, four isoflavanones, four neoflavones, two flavanones, tw...

The major flavonoids in rat serum after oral administration of Dalbergia odorifera extract were analyzed qualitatively and quantitatively by high performance liquid chromatography (HPLC) and its coupling to mass spectrometry (HPLC-MS). Utilizing HPLC-MS technique, 18 flavonoids including five isoflavones, four isoflavanones, four neoflavones, two f...

A reversed phase high performance liquid chromatography method was established for the simultaneous determination of eight major constituents, namely gallic acid, paeoniflorin sulfonate, catechin, albiflorin, paeoniflorin, benzoic acid, pentagalloylglucose and benzoylpaeoniflorin in red and white peony roots, the two commonly used traditional Chine...

A method incorporating high-performance liquid chromatography (HPLC) with electrospray ionization and tandem mass spectrometry, with parallel analysis by HPLC with UV detection using a diode-array detector, was developed for the qualitative characterization of flavonoids in D. odorifera. Twenty-three flavonoids, including six isoflavones, six neofl...